BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0124.0021.p (388 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SA1549 [O] COG0265 Trypsin-like serine proteases, typically peri... 159 1e-38 CAC2433 [O] COG0265 Trypsin-like serine proteases, typically per... 156 6e-38 BS_yyxA [O] COG0265 Trypsin-like serine proteases, typically per... 154 3e-37 CAC3218 [O] COG0265 Trypsin-like serine proteases, typically per... 151 2e-36 STM0571_2 [K] COG3933 Transcriptional antiterminator 150 4e-36 >SA1549 [O] COG0265 Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain Length = 424 Score = 159 bits (401), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 107/324 (33%), Positives = 172/324 (53%), Gaps = 34/324 (10%) Query: 69 AVPQIIKAVSPSVVGIIGKFTGDPGSGPDDRYNLTH--------GSGIIVKADG---WII 117 V ++IK+VSP++VG+I S DD GSG+I + + +I+ Sbjct: 84 TVHEMIKSVSPTIVGVINM---QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIV 140 Query: 118 TNAHVIKGLKNAVVVTSDAKSYNITASYLDEVSDLALVKI-NAKFLKPARFANAADKIQV 176 TN HVI G V + K D V+D+A++KI N K +K +FAN++ K+Q Sbjct: 141 TNNHVIDGANEIRVQLHNKKQVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSS-KVQT 199 Query: 177 GEKVIAIGTPISFSLRNSATVGVVSGLNRAVDA-------AYRLIQSDTAINPGNSGGPL 229 G+ V A+G P+ NS T G++S R +DA ++Q+D AINPGNSGG L Sbjct: 200 GDSVFAMGNPLGLQFANSVTSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGAL 259 Query: 230 VSMKGEVLGVNSLKYAAVGVENMGFSIPADTVQYIMNQLFQYGEVKRPSLGL------EL 283 V + G ++G+NS+K AA VE +GF+IP++ V+ + QL ++G++ RPS+G+ ++ Sbjct: 260 VDINGNLVGINSMKIAATQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDI 319 Query: 284 EESWSAIVGLPAQDPLTVTKVVSAEAEKAGIAAGDVLYAIDGHRVASLVDINEW-FKGYK 342 E + +D + V K S + GD++ IDG ++ VD+ + ++ K Sbjct: 320 PEEEREQLHTDREDGIYVAKADS----DIDLKKGDIITEIDGKKIKDDVDLRSYLYENKK 375 Query: 343 PGTAVKLLMQHDGDIVTRKLVLGQ 366 PG +V + + DG K+ L Q Sbjct: 376 PGESVTVTVIRDGKTKEVKVKLKQ 399 >CAC2433 [O] COG0265 Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain Length = 433 Score = 156 bits (394), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 113/317 (35%), Positives = 166/317 (52%), Gaps = 32/317 (10%) Query: 70 VPQIIKAVSPSVVGIIGK-----------FTGDPGSGPDDRYNLTHGSGIIVKADGWIIT 118 V QI+K VSP+VVG+ K F G+G + + GSGII DG+I+T Sbjct: 124 VSQIVKKVSPAVVGVSTKTTVTQNDFDSFFGSSNGNGSSTQEGM--GSGIIFNNDGYILT 181 Query: 119 NAHVIKGLKNAVVVTSDAKSYNITASYLDEVSDLALVKINAKFLKP--ARFANAADKIQV 176 N HVIKG V+ ++ K + DE +D+A++K+ F P A ++A + V Sbjct: 182 NYHVIKGADKIAVILNNKKEVSAKVVNYDEANDIAVIKMTGSFTVPGVAELGSSAS-LNV 240 Query: 177 GEKVIAIGTPISFSLRNSATVGVVSGLNRAVDAA----YRLIQSDTAINPGNSGGPLVSM 232 G+ V+AIG P+ + T GVVS +NR V + IQ+D AINPGNSGGPLV+ Sbjct: 241 GDSVVAIGNPLGKEFLGTVTTGVVSAVNREVAVSEGQKQTYIQTDAAINPGNSGGPLVNS 300 Query: 233 KGEVLGVNSLKYAAVGVENMGFSIPADTVQYIMNQLFQYGEVKRPSL-----GLELEESW 287 G+V+G+NS K + GVE +GFSIP DTV+ + L +P L G +++S Sbjct: 301 FGQVVGINSAKISENGVEGIGFSIPIDTVKSKIQNL------SKPILMLGISGEAVDKST 354 Query: 288 SAIVGLPAQDPLTVTKVVSAEAEKAGIAAGDVLYAIDGHRVASLVDINEWFKGYKPGTAV 347 + +P + + S+ A+KAG+ GDV+ DG +V S DI+ + G V Sbjct: 355 AEQHNIPQGVYIEQIQDFSS-AQKAGMQVGDVITKFDGKKVTSTSDIDSIKSKHNSGDTV 413 Query: 348 KLLMQHDGDIVTRKLVL 364 ++ + D T L L Sbjct: 414 QVEVYRDDAYKTLSLKL 430 >BS_yyxA [O] COG0265 Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain Length = 400 Score = 154 bits (388), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 112/335 (33%), Positives = 175/335 (52%), Gaps = 44/335 (13%) Query: 68 DAVPQIIKAVSPSVVGIIG-------KFTGDPGSGPDDRYNLTHGSGIIVKAD---GWII 117 +AV +I+ +SP+VVG++ +G+ GSG SG+I K + +++ Sbjct: 76 NAVTKIVSNMSPAVVGVVNIQKSDIWGESGEAGSG----------SGVIYKKNDHSAYVV 125 Query: 118 TNAHVIKGLKNAVVVTSDAKSYNITASYLDEVSDLALVKINAKFLKP-ARFANAADKIQV 176 TN HVI+G + D + D++ DLA++++ + +K A F N+ DK++ Sbjct: 126 TNHHVIEGASQIEISLKDGSRVSADLVGSDQLMDLAVLRVKSDKIKAVADFGNS-DKVKS 184 Query: 177 GEKVIAIGTPISFSLRNSATVGVVSGLNRAV----------DAAYRLIQSDTAINPGNSG 226 GE VIAIG P+ S T GV+SG RA+ D ++Q+D AINPGNSG Sbjct: 185 GEPVIAIGNPLGLEFAGSVTQGVISGTERAIPVDSNGDGQPDWNAEVLQTDAAINPGNSG 244 Query: 227 GPLVSMKGEVLGVNSLKYAAVGVENMGFSIPADTVQYIMNQLFQYGEVKRPSLGLELEES 286 G L++M G+V+G+NS+K A VE +G SIP+ V ++ L +YG+VKRP LG+E+ +S Sbjct: 245 GALLNMDGKVIGINSMKIAESAVEGIGLSIPSKLVIPVIEDLERYGKVKRPFLGIEM-KS 303 Query: 287 WSAIVGLPAQDPLTVTKVVS-----------AEAEKAGIAAGDVLYAIDGHRVASLVDIN 335 S I + L + K V+ + A KAG+ DV+ DG++V +VD+ Sbjct: 304 LSDIASYHWDETLKLPKNVTNGAVVMGVDAFSPAGKAGLKELDVITEFDGYKVNDIVDLR 363 Query: 336 EWFKGYKPGTAVKLLMQHDGDIVTRKLVLGQGDPL 370 + K G VK+ G + + L D L Sbjct: 364 KRLYQKKVGDRVKVKFYRGGKEKSVDIKLSSADQL 398 >CAC3218 [O] COG0265 Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain Length = 387 Score = 151 bits (381), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 109/320 (34%), Positives = 180/320 (56%), Gaps = 28/320 (8%) Query: 62 NTAVTADAVPQIIKAVSPSVVGIIGKFTGDPGSGPDDRYNLTHGSGIIVKADGWIITNAH 121 N++V ++V ++ + V PS+VGII + D GS +++ T SGII K+DG+I+TN H Sbjct: 71 NSSVPTNSVNKVSEEVGPSIVGIIN--SNDAGSDGEEQ---TISSGIIFKSDGYIVTNYH 125 Query: 122 VIKGLKNAVVVTSDAKS-YNITASY--LDEVSDLALVKINAKFLKPARFANAADKIQVGE 178 +I G +V S+AK+ I AS D SD+A++K+N+ L A F +++ K++ G+ Sbjct: 126 LINGANKVLVRLSNAKAGKEIEASLVGFDSASDIAIIKVNSHNLPTAIFGDSS-KVRAGD 184 Query: 179 KVIAIGTPISFSLRNSATVGVVSGLNRAVD---------AAYRLIQSDTAINPGNSGGPL 229 IAIG+ + S T G+VS NR + ++Y+++Q+D +IN NSGG L Sbjct: 185 LAIAIGSSLGNEASGSVTAGIVSSANRNLKLQDDANTQGSSYKVLQTDASINQINSGGAL 244 Query: 230 VSMKGEVLGVNSLKYAA-VGVENMGFSIPADTVQYIMNQLFQYGEVKRPSLGLELEESWS 288 + KGEV+GVNS K + E MGF+I + V+ I++Q+ + G+V +P +G+ Sbjct: 245 CNEKGEVIGVNSSKIGSQYNSEGMGFAISINQVKDIIDQIMKNGKVIKPFVGI-----VG 299 Query: 289 AIVGLPAQDPLT---VTKVV-SAEAEKAGIAAGDVLYAIDGHRVASLVDINEWFKGYKPG 344 + + +QD + V +VV + A KAG+ D++ ++G R+ S DI K G Sbjct: 300 GDIKVRSQDNMKGVYVKEVVPGSGAAKAGLRPSDIILELNGQRILSTNDIGSIVSSSKIG 359 Query: 345 TAVKLLMQHDGDIVTRKLVL 364 V + +G IV ++ L Sbjct: 360 DKVPCKVNRNGKIVKIQITL 379 >STM0571_2 [K] COG3933 Transcriptional antiterminator Length = 449 Score = 150 bits (379), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 14/281 (4%), Positives = 36/281 (12%), Gaps = 17/281 (6%) Query: 98 DRYNLTHGSGIIVKADGWIITNAHVIKGLKNAVVVTSDAKSYNITASYL--DEVSDLALV 155 +I A V + + V Sbjct: 74 AAIVSNAIDATSGGKVKALIIAHGYSTASSIAGVANRLIGEKI--YHAMDMPMEVAFSDV 131 Query: 156 KINAKFLK-PARFANAADKIQVGEKVIAIGTPISFSLRNSATVGVVSGLNRAV----DAA 210 + I + + VV + + + Sbjct: 132 SRAIVDYLQHTDTRAGVMVLIDMGYTKEIADALLS--VIHGPLVVVDNVTTRLALNVASE 189 Query: 211 YR--LIQSDTAINPGNSGGPLVSMKGEVLGVNSLKYAAVGVENMGFSIPADTVQYIMNQL 268 A + + ++ Sbjct: 190 IALQKNIEQIAEEIVPLNQSRWDVFWPAQKKARALLVTCITGIGTAFKFKNLMEKSQLTD 249 Query: 269 FQYGEVKRPSLG---LELEESWSAIVGLPAQDPLTVTKVVS-AEAEKAGIAAGDVLYAID 324 F + + S + A ++ + + Sbjct: 250 FDINIIACEYTRLKNSRMAASLLNQYEVIAVVGTIDPQLAGVPWVGIEELLGEQGYAHLS 309 Query: 325 GHRVASLVDINEWFKGYKPGTAVKLLMQHDGDIVTRKLVLG 365 L D L + + Sbjct: 310 QLLSGYLNDKQIALINKNMVREFSLHNVVNSLTILNANKTI 350 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.133 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,563,923 Number of extensions: 1127902 Number of successful extensions: 2981 Number of sequences better than 1.0e-05: 94 Number of HSP's gapped: 2682 Number of HSP's successfully gapped: 94 Length of query: 388 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 280 Effective length of database: 25,922,359 Effective search space: 7258260520 Effective search space used: 7258260520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 117 (49.7 bits)