BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0124.0012.p
         (130 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SA0760 [E] COG0509 Glycine cleavage system H protein (lipoate-bi...   124   3e-29
STM0212 [GEPR] COG0477 Permeases of the major facilitator superf...   108   2e-24
aq_1108 [E] COG0509 Glycine cleavage system H protein (lipoate-b...   107   5e-24
PA5214 [E] COG0509 Glycine cleavage system H protein (lipoate-bi...   104   3e-23
SSO0920 [E] COG0509 Glycine cleavage system H protein (lipoate-b...   102   1e-22

>SA0760 [E] COG0509 Glycine cleavage system H protein (lipoate-binding)
          Length = 126

 Score =  124 bits (311), Expect = 3e-29,   Method: Compositional matrix adjust.
 Identities = 65/127 (51%), Positives = 84/127 (66%), Gaps = 3/127 (2%)

Query: 4   VLDNLLYSEEHEWAQQGEGRVVRIGITDHAQHLLGDIVFVEFPEVGAAISAGDSVGSIES 63
           V + L YS+EHEW +  EG V  IGIT++AQ  LGDIVFVE PE    I+ GD+ GS+ES
Sbjct: 3   VPNELKYSKEHEWVKV-EGNVATIGITEYAQSELGDIVFVELPETDDEINEGDTFGSVES 61

Query: 64  VKTVSELYSPVSGTVTKINDTLEASPELINDQPYSGGWIFELEISGEFXXXXXXXXXXXX 123
           VKTVSELY+P+SG V ++N+ LE SPE +N+ PY   W+ ++EIS E             
Sbjct: 62  VKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVKVEISDE--SQLEALLTAEK 119

Query: 124 YRELIGE 130
           Y E+IGE
Sbjct: 120 YSEMIGE 126


>STM0212 [GEPR] COG0477 Permeases of the major facilitator superfamily
          Length = 433

 Score =  108 bits (269), Expect = 2e-24,   Method: Compositional matrix adjust.
 Identities = 12/101 (11%), Positives = 20/101 (19%), Gaps = 1/101 (0%)

Query: 10  YSEEHEWAQQGEGRVVRIGITDHAQHLLGDIVFVEFPEVGAAISAGDSVGSIESVKTVSE 69
            +               I   D   + L   +         A     ++ S  S      
Sbjct: 6   NATIRRVVVS-AWIGNSIEYYDFLLYGLASALVFGPLFFPGASPLTATLSSFASFGVGFI 64

Query: 70  LYSPVSGTVTKINDTLEASPELINDQPYSGGWIFELEISGE 110
                +       DTL     L+      G   F +     
Sbjct: 65  SRPLGALFFGNRGDTLGRKNTLLITLGGMGAVTFLIGCLPS 105


>aq_1108 [E] COG0509 Glycine cleavage system H protein (lipoate-binding)
          Length = 171

 Score =  107 bits (267), Expect = 5e-24,   Method: Compositional matrix adjust.
 Identities = 48/99 (48%), Positives = 70/99 (70%), Gaps = 1/99 (1%)

Query: 10  YSEEHEWAQQGEGRVVRIGITDHAQHLLGDIVFVEFPEVGAAISAGDSVGSIESVKTVSE 69
           Y++EHEW +   G    +GITD+AQ  LGDIV+V+ PE G  + AGD++ +IESVK V+ 
Sbjct: 17  YTKEHEWVRVKNG-FAEVGITDYAQKQLGDIVYVDLPEKGKEVDAGDTLANIESVKNVAP 75

Query: 70  LYSPVSGTVTKINDTLEASPELINDQPYSGGWIFELEIS 108
           +Y+PV+GTV ++N+ L+  P +IND PY  GWI  +E+ 
Sbjct: 76  VYAPVTGTVVEVNEDLKDEPGIINDDPYEAGWIAVIEMK 114


>PA5214 [E] COG0509 Glycine cleavage system H protein (lipoate-binding)
          Length = 129

 Score =  104 bits (260), Expect = 3e-23,   Method: Compositional matrix adjust.
 Identities = 49/108 (45%), Positives = 70/108 (64%), Gaps = 1/108 (0%)

Query: 1   MSEVLDNLLYSEEHEWAQQGEGRVVRIGITDHAQHLLGDIVFVEFPEVGAAISAGDSVGS 60
           MS +   L Y++ HEWA+      V +GI+DHAQ  LGD+VF+E PE+G  ++AG   G 
Sbjct: 1   MSNIPAELRYAQSHEWARLEADGSVTVGISDHAQEALGDVVFIELPELGKTLAAGQEAGV 60

Query: 61  IESVKTVSELYSPVSGTVTKINDTLEASPELINDQPYSGGWIFELEIS 108
           +ESVK  S++YSP+ G V  IN+ L  +PE +N+ PY   W F+L+ S
Sbjct: 61  VESVKAASDIYSPIGGEVIAINEALADTPEDVNNDPYV-SWFFKLKPS 107


>SSO0920 [E] COG0509 Glycine cleavage system H protein (lipoate-binding)
          Length = 137

 Score =  102 bits (255), Expect = 1e-22,   Method: Compositional matrix adjust.
 Identities = 49/105 (46%), Positives = 69/105 (65%)

Query: 4   VLDNLLYSEEHEWAQQGEGRVVRIGITDHAQHLLGDIVFVEFPEVGAAISAGDSVGSIES 63
           VL + LY+E  EW       V  IGITD+AQ  L DIV +E P++   + AG+SVG IES
Sbjct: 11  VLTDRLYTETDEWVVLSNDNVAVIGITDYAQKKLRDIVGIELPQLQKEVKAGESVGVIES 70

Query: 64  VKTVSELYSPVSGTVTKINDTLEASPELINDQPYSGGWIFELEIS 108
           VK  ++++SP+SG + ++N+ L   PE+IN  PY  GWIF+L+ S
Sbjct: 71  VKAAADIFSPLSGIIVEVNNKLLEHPEIINKDPYGEGWIFKLKAS 115


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.313    0.134    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 9,264,279
Number of extensions: 320721
Number of successful extensions: 574
Number of sequences better than 1.0e-05: 13
Number of HSP's gapped: 572
Number of HSP's successfully gapped: 13
Length of query: 130
Length of database: 38,378,431
Length adjustment: 94
Effective length of query: 36
Effective length of database: 27,537,035
Effective search space: 991333260
Effective search space used: 991333260
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 109 (46.6 bits)