BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0124.0011.p (374 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA5215 [E] COG0404 Glycine cleavage system T protein (aminomethy... 249 4e-66 VNG1606G [E] COG0404 Glycine cleavage system T protein (aminomet... 236 6e-62 RSc3293 [E] COG0404 Glycine cleavage system T protein (aminometh... 235 8e-62 ZgcvT [E] COG0404 Glycine cleavage system T protein (aminomethyl... 229 5e-60 >PA5215 [E] COG0404 Glycine cleavage system T protein (aminomethyltransferase) Length = 360 Score = 249 bits (637), Expect = 4e-66, Method: Compositional matrix adjust. Identities = 159/371 (42%), Positives = 216/371 (58%), Gaps = 24/371 (6%) Query: 6 RTPFYDLYSAYAESRCIDFGGWELPVQFTGIVKEHEAVRRQAGLFDVSHMGEFMVSGSGS 65 RTP Y+L+ A ++ +DFGGW++P+ + V+EH VRR G+FDVSHM V+G + Sbjct: 4 RTPLYELHVALG-AKMVDFGGWDMPLHYGSQVEEHHQVRRDCGVFDVSHMTVVDVAGEQA 62 Query: 66 EAFLQLMTTNDVSRL-QDGGAQYTLMLYPTGGVVDDLLVYRLGEERYMLVVNASNIDKDF 124 A+LQ + NDV+RL + G A Y+ ML GGVVDDL+VY L E Y +VVNAS DKD Sbjct: 63 TAYLQHLLANDVARLGETGKALYSAMLNEEGGVVDDLIVY-LTEHGYRVVVNASTRDKDI 121 Query: 125 EWLQEHLTDEFSGVTLTNVSDETLLLALQGPLAETILAEVTS----APIAELAPFHFIER 180 W+Q F V L D +LA+QGP A +E+ S A I EL PF Sbjct: 122 AWMQAQAAG-FK-VDLQERGD-LAMLAIQGPNARVHSSELVSPARAALIRELKPFQGRAE 178 Query: 181 AVVCGVEVLLSRTGYTGEDGFELYAPQDTAAVLWNGLLAAGAPHGLTPAGLGARDTLRFE 240 + ++RTGYTGEDG E+ P A N L+ A G++PAGLGARDTLR E Sbjct: 179 G-----DWFIARTGYTGEDGLEIMLPAAEAPGFLNELVGA----GISPAGLGARDTLRLE 229 Query: 241 AKLPLYGQELSADITPLEAGVQFFVKLDKAG--FIGRDALLKQKESGLPRRLVGLEMIDR 298 A L LYGQ++ ++PL A + + V + F+GR AL QK +G +LVGL + +R Sbjct: 230 AGLNLYGQDMDESVSPLAANMGWTVAWEPVARDFVGRRALEAQKAAGDQPKLVGLVLEER 289 Query: 299 GIPRSHYPVYADGVKIGEVTTGTQSPTLKRNLGLALLDAAYTETGTEVYVEIRGKQLKAA 358 G+ R+H V G+ GE+T+G+ SPTL +++ LA + AA TG VEIRGK Sbjct: 290 GVLRAHQVVRVAGIGEGEITSGSFSPTLNKSIALARVPAA---TGDRAEVEIRGKWYPVR 346 Query: 359 VVKTPFYKKSQ 369 VV+ F + + Sbjct: 347 VVQPSFVRHGK 357 >VNG1606G [E] COG0404 Glycine cleavage system T protein (aminomethyltransferase) Length = 387 Score = 236 bits (601), Expect = 6e-62, Method: Compositional matrix adjust. Identities = 144/371 (38%), Positives = 202/371 (54%), Gaps = 17/371 (4%) Query: 4 LKRTPFYDLYSAYAESRCIDFGGWELPVQFTGIVKEHEAVRRQAGLFDVSHMGEFMVSGS 63 L+ +P + + S +FGGW +PV F GI EH AVR AG+FDVSHMGE VSG Sbjct: 27 LRTSPLHGRHEDRGAS-FTEFGGWNMPVDFDGIQAEHAAVREAAGIFDVSHMGEIEVSGP 85 Query: 64 GSEAFLQLMTTNDVSRLQDGGAQYTLMLYPTGGVVDDLLVYRLGEE---RYMLVVNASNI 120 +E +Q +TTNDVSRL G AQY + G ++DD +VYR + ++ V NA + Sbjct: 86 DAERLMQRLTTNDVSRLDPGDAQYAAITDDDGIMIDDTVVYRTPADWPGAFLFVPNAGHD 145 Query: 121 DKDFEWLQEHLTDEFSGVTLTNVSDETLLLALQGPLAETILAEVTSAPIAELAPFHFIER 180 F+ +H ++ NV+ + ++A+QGP A ++A + +L F Sbjct: 146 AAAFDRWTDHRDAHDLDASVDNVTTDYGMVAVQGPDAPDLVAARAGDGVHDLGRFEAATV 205 Query: 181 AVVCGVEVLLSRTGYTGEDGFELYAPQDTAAVLWNGLLAAGAPHGLTPAGLGARDTLRFE 240 V GVE L++ TGYTGE G E+ P D A +W+ + + P GLGARDTLR E Sbjct: 206 G-VAGVECLVANTGYTGEAGVEIVFPADGAGAVWDAIA-----NDCQPCGLGARDTLRME 259 Query: 241 AKLPLYGQELSAD---ITPLEAGVQFFVKLDKAGFIGRDALLKQKESGLPRRLVGLEMID 297 L GQ+ + TP EAG+ F V ++GF+GRDAL ++ VGL + + Sbjct: 260 HGFLLSGQDFDPEENPRTPFEAGIGFAVAP-ESGFVGRDALADTDSP--EQQFVGLTLDE 316 Query: 298 RGIPRSHYPVYAD-GVKIGEVTTGTQSPTLKRNLGLALLDAAYTETGTEVYVEIRGKQLK 356 RG+PR Y V G +IG VT+GT SPTL +GL +D+A+ GT V V IRG + Sbjct: 317 RGVPRHGYAVTTPAGDEIGTVTSGTMSPTLGEPIGLGYVDSAHAADGTTVAVRIRGTDKQ 376 Query: 357 AAVVKTPFYKK 367 A + PF + Sbjct: 377 ATITTPPFLDQ 387 >RSc3293 [E] COG0404 Glycine cleavage system T protein (aminomethyltransferase) Length = 375 Score = 235 bits (600), Expect = 8e-62, Method: Compositional matrix adjust. Identities = 153/382 (40%), Positives = 219/382 (57%), Gaps = 25/382 (6%) Query: 4 LKRTPFYDLYSAYAESRCIDFGGWELPVQFTGIVKEHEAVRRQAGLFDVSHMGEFMVSGS 63 L+ TP ++ + +R +DFGGW++PV + ++EH AVRR AG+FDVSHM ++G+ Sbjct: 3 LQHTPLNAIHRSLG-ARMVDFGGWDMPVNYGSQIEEHHAVRRDAGIFDVSHMCVVDLTGA 61 Query: 64 GSEAFLQLMTTNDVSRLQD-GGAQYTLMLYPTGGVVDDLLVYRLGEERYMLVVNASNIDK 122 FL+ + N++ +LQ G A YT ML P GGV+DDL+VY E+ + LVVNA Sbjct: 62 RVRDFLRGLLANNIDKLQTPGKALYTCMLNPKGGVIDDLIVYFFREDWFRLVVNAGTAPT 121 Query: 123 DFEWLQEHLTDEFSGVTLT------NVSDETL-LLALQGPLAETILAEV---TSAPIAEL 172 D EW+ +GV +T N E L ++A+QGP A T A L Sbjct: 122 DIEWITAQNAAAGTGVAITPRRSDNNAGAEPLGIVAVQGPNARAKAYAALPGTQAVGEAL 181 Query: 173 APFHFIERAVVCGV-EVLLSRTGYTGEDGFELYAPQDTAAVLWNGLLAAGAPHGLTPAGL 231 PF+ A + GV E++++RTGYTGEDGFEL P A +W LL A G+ P GL Sbjct: 182 KPFN-AGFATIDGVGEIMVARTGYTGEDGFELVVPAAQIAGVWERLLQA----GVRPCGL 236 Query: 232 GARDTLRFEAKLPLYGQELSADITPLEAGVQFFVKL-DKAGFIGRDALLKQKESGLPRRL 290 GARDTLR EA + LYGQ++ ++PL+AG+ + V L + F G+ AL +G + Sbjct: 237 GARDTLRLEAGMNLYGQDMDEHVSPLDAGLAWTVDLQSERDFNGKAAL---AANGQRDQF 293 Query: 291 VGLEMIDR-GIPRSHYPVYADGVKIGEVTTGTQSPTLKRNLGLALLDAAYTETGTEVYVE 349 VGL + D+ G+ R+H V GE+T+GT SP+L +++ LA L G +V VE Sbjct: 294 VGLLLRDKGGVLRAHQKVIT-AAGDGEITSGTFSPSLSQSIALARL-PKDVAIGADVQVE 351 Query: 350 IRGKQLKAAVVKTPFYKKSQGV 371 IR ++L A VVK PF + + + Sbjct: 352 IRDRRLTATVVKLPFVRNGKAL 373 >ZgcvT [E] COG0404 Glycine cleavage system T protein (aminomethyltransferase) Length = 364 Score = 229 bits (584), Expect = 5e-60, Method: Compositional matrix adjust. Identities = 139/378 (36%), Positives = 217/378 (57%), Gaps = 28/378 (7%) Query: 5 KRTPFYDLYSAYAESRCIDFGGWELPVQFTGIVKEHEAVRRQAGLFDVSHMGEFMVSGSG 64 ++TP Y+ ++ +R +DF GW +P+ + + EH VR AG+FDVSHM + GS Sbjct: 3 QQTPLYEQHT-LCGARMVDFHGWMMPLHYGSQIDEHHEVRTDAGMFDVSHMTIVDLRGSR 61 Query: 65 SEAFLQLMTTNDVSRL-QDGGAQYTLMLYPTGGVVDDLLVYRLGEERYMLVVNASNIDKD 123 + FL+ + NDV++L + G A Y+ ML +GGV+DDL+VY E+ + LVVN++ +KD Sbjct: 62 TREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVYYFTEDFFRLVVNSATREKD 121 Query: 124 FEWLQEHLTDEFSGVTLTNVSDETLLLALQGPLAE----TILAEVTSAPIAELAPFHFIE 179 W+ +H E G+ +T V D+ ++A+QGP A+ T+ + + + PF ++ Sbjct: 122 LSWITQHA--EPFGIEIT-VRDDLSMIAVQGPNAQAKAATLFNDAQRQAVEGMKPFFGVQ 178 Query: 180 RAVVCGVEVLLSRTGYTGEDGFELYAPQDTAAVLWNGLLAAGAPHGLTPAGLGARDTLRF 239 ++ ++ TGYTGE G+E+ P + AA W L+ G+ P GLGARDTLR Sbjct: 179 AG-----DLFIATTGYTGEAGYEIALPNEKAADFWRALVEG----GVKPCGLGARDTLRL 229 Query: 240 EAKLPLYGQELSADITPLEAGVQFFVKLDKA--GFIGRDALLKQKESGLPRRLVGLEMID 297 EA + LYGQE+ I+PL A + + + + A FIGR+AL Q+E G +LVGL M + Sbjct: 230 EAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREHG-TEKLVGLVMTE 288 Query: 298 RGIPRSHYPVY---ADGVKI-GEVTTGTQSPTLKRNLGLALLDAAYTETGTEVYVEIRGK 353 +G+ R+ PV A G + G +T+GT SPTL ++ LA + ET V+IR + Sbjct: 289 KGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPKGIGETAI---VQIRNR 345 Query: 354 QLKAAVVKTPFYKKSQGV 371 ++ V K F + + V Sbjct: 346 EMPVKVTKPVFVRNGKAV 363 Score = 226 bits (575), Expect = 6e-59, Method: Compositional matrix adjust. Identities = 139/378 (36%), Positives = 217/378 (57%), Gaps = 28/378 (7%) Query: 5 KRTPFYDLYSAYAESRCIDFGGWELPVQFTGIVKEHEAVRRQAGLFDVSHMGEFMVSGSG 64 ++TP Y+ ++ +R +DF GW +P+ + + EH VR AG+FDVSHM + GS Sbjct: 3 QQTPLYEQHT-LCGARMVDFHGWMMPLHYGSQIDEHHEVRTDAGMFDVSHMTIVDLRGSR 61 Query: 65 SEAFLQLMTTNDVSRL-QDGGAQYTLMLYPTGGVVDDLLVYRLGEERYMLVVNASNIDKD 123 + FL+ + NDV++L + G A Y+ ML +GGV+DDL+VY E+ + LVVN++ +KD Sbjct: 62 TREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVYYFTEDFFRLVVNSATREKD 121 Query: 124 FEWLQEHLTDEFSGVTLTNVSDETLLLALQGPLAE----TILAEVTSAPIAELAPFHFIE 179 W+ +H E G+ +T V D+ ++A+QGP A+ T+ + + + PF ++ Sbjct: 122 LSWITQHA--EPFGIEIT-VRDDLSMIAVQGPNAQAKAATLFNDAQRQAVEGMKPFFGVQ 178 Query: 180 RAVVCGVEVLLSRTGYTGEDGFELYAPQDTAAVLWNGLLAAGAPHGLTPAGLGARDTLRF 239 ++ ++ TGYTGE G+E+ P + AA W L+ G+ P GLGARDTLR Sbjct: 179 AG-----DLFIATTGYTGEAGYEIALPNEKAADFWRALVEG----GVKPCGLGARDTLRL 229 Query: 240 EAKLPLYGQELSADITPLEAGVQFFVKLDKA--GFIGRDALLKQKESGLPRRLVGLEMID 297 EA + LYGQE+ I+PL A + + + + A FIGR+AL Q+E G +LVGL M + Sbjct: 230 EAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREHG-TEKLVGLVMTE 288 Query: 298 RGIPRSHYPVY---ADGVKI-GEVTTGTQSPTLKRNLGLALLDAAYTETGTEVYVEIRGK 353 +G+ R+ PV A G + G +T+GT SPTL ++ LA + ET V+IR + Sbjct: 289 KGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPKGIGETAI---VQIRNR 345 Query: 354 QLKAAVVKTPFYKKSQGV 371 ++ V K F + + V Sbjct: 346 EMPVKVTKPVFVRNGKAV 363 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.137 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,187,396 Number of extensions: 1221706 Number of successful extensions: 1951 Number of sequences better than 1.0e-05: 35 Number of HSP's gapped: 1835 Number of HSP's successfully gapped: 35 Length of query: 374 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 266 Effective length of database: 25,922,359 Effective search space: 6895347494 Effective search space used: 6895347494 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)