BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0124.0005.p (386 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPy0746 [V] COG0842 ABC-type multidrug transport system, permeas... 107 2e-23 CAC0243 [V] COG0842 ABC-type multidrug transport system, permeas... 105 2e-22 BS_yfiN [V] COG0842 ABC-type multidrug transport system, permeas... 104 3e-22 RSc0165 [V] COG0842 ABC-type multidrug transport system, permeas... 92 1e-18 BS_yfiM [V] COG0842 ABC-type multidrug transport system, permeas... 82 2e-15 >SPy0746 [V] COG0842 ABC-type multidrug transport system, permease component Length = 372 Score = 107 bits (268), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 70/304 (23%), Positives = 136/304 (44%), Gaps = 12/304 (3%) Query: 56 DTGAAGRHLLAELENTGDYKLIARNDEAALKEGIIQQEGAAGISIPAEYTAQLAHGQQPE 115 D + +A+L+ Y + + + + + + A ++I ++ ++ G+ + Sbjct: 52 DNSQISKQFIAQLKQNKKYDIFTKIKKEHIDHYLQDKSLEAVLTIDKGFSDKVLQGKSQK 111 Query: 116 LSVYELRASETSILVKMKVNTIAGEMLDTARTVNAVNGGAGDAEAKLGEILKQAEQHNVG 175 L++ + SE + VK + N +L+ + V G D IL++ +Q N Sbjct: 112 LNIRSIANSEITEWVKAQTN----YLLENYNIIGDVALGNEDT---FNRILQKNQQLNYD 164 Query: 176 S---TRTDYDLYPRQTLGVITGLTLMFLMGLVTSTVSLIMDDRKGRTMMRMFSAPVRSYE 232 T TD + TG L+ ++G + S I+ D+ + R+ + + + Sbjct: 165 VKQVTLTDRS-RSKAVSSTTTGFLLILMLGSTSVIYSGILADKSSQLYHRLMLSNLSRFR 223 Query: 233 IALGNFLGSCMVGILQIAVVLTLGKWVLRYDYEVPMYLYFLVLAAFMLVSMGIASTVAGL 292 L + +QI ++L+L K V + VP L ++ F L+++G + + Sbjct: 224 YMLSYVCVGFVAFTIQIVIMLSLLK-VFNISFFVPTSLLLIIFFLFSLLAIGFGLLIGAI 282 Query: 293 VRNPGNAGMLNALILTPTCMLGGCFWPISVMPDYMQKAANFVPQKWAIQAVDIAAAGGSW 352 +N + L LI+ PT ML GC WP+S+ P YMQ +PQ W + A+ I +GG+ Sbjct: 283 TQNSQQSSQLANLIVMPTSMLAGCLWPLSITPSYMQAIGKLLPQNWVLSAIAIFQSGGTL 342 Query: 353 NELW 356 ++ W Sbjct: 343 SQAW 346 >CAC0243 [V] COG0842 ABC-type multidrug transport system, permease component Length = 370 Score = 105 bits (261), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 87/356 (24%), Positives = 162/356 (45%), Gaps = 17/356 (4%) Query: 5 LAIAWNMVKRTIASRKGMLTYILLPSIVIAAIISVTGGMEGDKPTVLYSNMDTGAAGRHL 64 + I +N +KR + ++ L P ++ I + G TV + D + Sbjct: 1 MTIFFNNIKRIFRKKANIIVMFLFP--ILFISIISSISSGGSGFTVGVVDNDNTKLTSMI 58 Query: 65 LAELENTGDYKLIARNDEAALKEGIIQQEGAAGISIPAEYTAQLAHGQQPE-LSVYELRA 123 ++ G K++ + D + + II ++ I IP T + + E + +Y ++ Sbjct: 59 KERIKANGTIKIVNKKD---ISDYIIDKKIDMAIVIPKGLTKNIIGNSKGENIEIYGIKG 115 Query: 124 SETSILVKMKVNTIAGEMLDTARTVNAVNGGAGDAEAKLGEILKQAEQHN-VGSTR-TDY 181 + ++ VN+ + A+ GD + K +K ++ N V T+ T+ Sbjct: 116 TSNDSSMEYYVNSFVNAAQNIAK------AAKGD-KTKFYNGVKNYQKGNFVSETKYTNG 168 Query: 182 DLYPRQTLGVITGLTLMFLMGLVTSTVSLIMDDRKGRTMMRMFSAPVRSYEIALGNFLGS 241 + ++T G + ++ L T +LI+ D+K RMF++ V + +G+ Sbjct: 169 EREKQKTSTTSIGFLIASIIYLSTMITTLILVDKKDGVYNRMFASGVNRFRYIVGSMFSF 228 Query: 242 CMVGILQIAVVLTLGKWVLRYDY-EVPMYLYFLVLAAFMLVSMGIASTVAGLVRNPGNAG 300 V ++QIA V + K+V++ D P+ LY LVL F + + + ++ ++ A Sbjct: 229 VAVNLIQIAGVFLVMKYVVKIDLGPSPIKLY-LVLVVFGIACVSLGVLISNRCKDLKQAN 287 Query: 301 MLNALILTPTCMLGGCFWPISVMPDYMQKAANFVPQKWAIQAVDIAAAGGSWNELW 356 + LI TP MLGGC+WP VM +Q+ +FVP WA+ AVD GGS + ++ Sbjct: 288 SMTVLISTPVAMLGGCYWPKEVMGSTLQRIGDFVPSTWAMNAVDKIINGGSLSSIY 343 >BS_yfiN [V] COG0842 ABC-type multidrug transport system, permease component Length = 385 Score = 104 bits (259), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 93/368 (25%), Positives = 153/368 (41%), Gaps = 29/368 (7%) Query: 1 MNKILAIAWNMVKRTIASRKGMLTYILLPSIVIAAIISVTGGMEGDKPTVLYSNMDTGAA 60 M KILAI + + L P ++ S+ G + DK + + D Sbjct: 1 MKKILAICGIELSLIFKKPQNYLIMFAAPLLLTFVFGSMLSGND-DKVRLAIVDQDDTIL 59 Query: 61 GRHLLAELENTGDYKLIARNDEAALKEGIIQQEGAAGISIPAEYTAQLAHGQQPELSV-Y 119 +H + +L+ D + E+ E + Q++ A I I + QL G+ PEL + Sbjct: 60 SQHYIRQLKAHDDMYVFENMSESKASEKLKQKKIAGIIVISRSFQTQLEKGKHPELIFRH 119 Query: 120 ELRASETSILVKMKVNTIAGEMLDTARTVNAVNGGAGDAEAKLGEILKQAEQHNVGSTRT 179 SE ++ + + +A T+N A A GE K A + + Sbjct: 120 GPELSEAPMVKQYAESALA--------TLNIQVTAAKTASQTAGENWKAA--YKTVFAKK 169 Query: 180 DYDLYP---RQTLG-------------VITGLTLMFLMGLVTSTVSLIMDDRKGRTMMRM 223 D+ P RQTL G +++F+M + I++ RK R+ Sbjct: 170 HEDIVPAVTRQTLSDKKEGAEASDTASRAAGFSILFVMLTMMGAAGTILEARKNGVWSRL 229 Query: 224 FSAPVRSYEIALGNFLGSCMVGILQIAVVLTLGKWVLRYDYEVPMYLYFLVLAAFMLVSM 283 +A V EI G L ++G +Q ++L W+ ++ P + LV + F+L + Sbjct: 230 LTASVSRAEIGAGYVLSFFVIGWIQFGILLLSTHWLFGINWGNPAAVIVLV-SLFLLTVV 288 Query: 284 GIASTVAGLVRNPGNAGMLNALILTPTCMLGGCFWPISVMPDYMQKAANFVPQKWAIQAV 343 GI +A VR P L + TCM+ G +WPI + P +MQ A F+PQKWA+ + Sbjct: 289 GIGLMIAANVRTPEQQLAFGNLFVIATCMVSGMYWPIDIEPKFMQSIAEFLPQKWAMSGL 348 Query: 344 DIAAAGGS 351 A G+ Sbjct: 349 TEIIANGA 356 >RSc0165 [V] COG0842 ABC-type multidrug transport system, permease component Length = 381 Score = 92.0 bits (227), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 84/358 (23%), Positives = 164/358 (45%), Gaps = 26/358 (7%) Query: 9 WNMV-KRTIASRKGMLTYILLPSIVIAAIISVTGGMEGDK---PTVLYSNMDTGAAGRHL 64 W++V K + R+ +T+ ++ I I ++ + D PT + + D R Sbjct: 14 WSIVLKEFLQLRRDRVTFAMMIGIPIMQLLMFGFAINTDPKHLPTGVIAA-DRSEFTRSF 72 Query: 65 LAELENTGDYKLIAR-NDEAALKEGIIQQEGAAGISIPAEYTAQLAHGQQPELSVYELRA 123 LA L NT ++L DEAA ++ + + +SIP ++T +L G++P L V E A Sbjct: 73 LASLRNTDYFRLDETLPDEAAGRQALAEGRLQFVVSIPPDFTRRLVRGERPALLV-EADA 131 Query: 124 SETSI--LVKMKVNTIAGEML--DTARTVNAVNGGAGDAEAKLGEILKQAEQHNVGSTRT 179 ++ S L V +A ++ D + + GG E ++ + +N T Sbjct: 132 TDPSATGLALASVTQLAQGVVSKDMKGVLAPLAGGQPPFEVRVHRL------YNPEGI-T 184 Query: 180 DYDLYPRQTLGVITGLTLMFLMGLVTSTVSLIMDDRKGRTMMRMFSAPVRSYEIALGNFL 239 Y++ P +GVI +T++ + GL + +R+ TM + + PVR E+ G + Sbjct: 185 QYNIIP-GLMGVILTMTMVMMTGLAMT------RERERGTMENLLATPVRPLEVMTGKIV 237 Query: 240 GSCMVGILQIAVVLTLGKWVLRYDYEVPMYLYFLVLAAFMLVSMGIASTVAGLVRNPGNA 299 +G++Q++++L +++ + ++ +L F++ S+ + T++ L +N A Sbjct: 238 PYIFIGLVQVSIILLAARFIFHVPFVGSAWMVYLAALLFIVASLTVGITLSSLAQNQLQA 297 Query: 300 GMLNALILTPTCMLGGCFWPISVMPDYMQKAANFVPQKWAIQAVD-IAAAGGSWNELW 356 L P+ +L G +P + MP + Q + +P + + I G W ELW Sbjct: 298 TQLTFFYFLPSILLSGFMFPFAGMPKWAQVIGDVLPMTYFHRLTRGILLKGNGWVELW 355 >BS_yfiM [V] COG0842 ABC-type multidrug transport system, permease component Length = 90 Score = 82.0 bits (201), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 0/383 (0%), Positives = 0/383 (0%), Gaps = 34/383 (8%) Query: 1 MNKILAIAWNMVKRTIASRKGMLTYILLPSIVI----AAIISVT-GGMEGDKPTVLYSNM 55 M + + IAS + + + L+ + Sbjct: 1 MANDSWSGQDKAQHFIASAMLSAAGNEYSQHQGMSRDRSAMFGLMFSVSLGASKELWDSR 60 Query: 56 DTGAAGRHLLAE-LENTGDYKLIARNDEAALKEGIIQQEGAAGISIPAEYTA-------Q 107 G+ Sbjct: 61 PEGSGWSWKDLAW 120 Query: 108 LAHGQQPELSVYELRASETSILVKMK-VNTIAGEMLDTARTVNAVNGGAGDA-------- 158 Sbjct: 121 -- 178 Query: 159 ---EAKLGEILKQAEQHNVGSTRTDYDLYPRQTLGVITGLTLMFLMGLVTSTVSLIMDDR 215 Sbjct: 179 ------- 231 Query: 216 KGRTMMRMFSAPVRSYEIALGNFLGSCMVGILQIAVVLTLGKWVLRYDYEVPMYLYFLVL 275 Sbjct: 232 291 Query: 276 AAFMLVSMGIASTVAGLVRNPGNAGMLNALILTPTCMLGGCFWPISVMPDYMQKAANFVP 335 Sbjct: 292 351 Query: 336 QKWAIQAVDIAAAGGSWNELWLP 358 Sbjct: 352 374 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.135 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,963,612 Number of extensions: 1122139 Number of successful extensions: 2306 Number of sequences better than 1.0e-05: 31 Number of HSP's gapped: 2334 Number of HSP's successfully gapped: 31 Length of query: 386 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 278 Effective length of database: 25,922,359 Effective search space: 7206415802 Effective search space used: 7206415802 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 117 (49.7 bits)