BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0124.0002.p (387 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yfiJ [T] COG4585 Signal transduction histidine kinase 70 8e-12 CAC0864 [T] COG4585 Signal transduction histidine kinase 69 1e-11 CAC2940 [T] COG4585 Signal transduction histidine kinase 69 2e-11 BH0784 [T] COG4585 Signal transduction histidine kinase 68 2e-11 CAC1454 [T] COG4585 Signal transduction histidine kinase 60 7e-09 >BS_yfiJ [T] COG4585 Signal transduction histidine kinase Length = 400 Score = 69.7 bits (169), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 56/230 (24%), Positives = 106/230 (46%), Gaps = 10/230 (4%) Query: 110 ALSGSEPQVRVVMNLTFLLSAALNKLLMRSGRGRVDTLFL-YDELRKKHFELEEARSSLL 168 A+ G+E + + ++TF++ + L+R DT + EL + H L A L Sbjct: 119 AMFGNEFVIDHLTSMTFVVFCGVVGKLIRKLLDAQDTAKQQFQELTESHLALSAAHQELH 178 Query: 169 QFTXXXXXXXXXXXXXXXXXXLHDDIGHR----LIRVKMMTEAAIHTLPHSQETGMEMMN 224 + +HD +GH+ L++++++ E + ET Sbjct: 179 LYAKQVEELTAIYERNRMAREIHDTVGHKMTALLVQLQLLREWQKRDSQKADETVGVCET 238 Query: 225 QIRDQLAASMDDMRAAVRRINYGSQLEGAYALDRLLEELGRDTGIETSYQVHGMPYPLYP 284 R+ ++DD+R +VR + + +L +L E+ ++ G+ T + V G P + Sbjct: 239 LARE----ALDDVRLSVRTLQTENDPSLIESLKQLTEDFCKNAGVTTEFAVSGDPAIIPL 294 Query: 285 SVRVTLYKNAQEALTNAIRHGRATAIWINVSFSEQEVAMEVGNNGQ-RPE 333 S+ TL + QEALTNA RHG A A I ++ + +++ + ++G+ PE Sbjct: 295 SLHPTLIRTVQEALTNAKRHGGAAACSIQLACTTDSISLVIKDDGKGNPE 344 >CAC0864 [T] COG4585 Signal transduction histidine kinase Length = 392 Score = 68.9 bits (167), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 56/241 (23%), Positives = 101/241 (41%), Gaps = 10/241 (4%) Query: 149 LYDELRKKHFELEEARSSLLQFTXXXXXXXXXXXXXXXXXXLHDDIGHRLIRVKMMTEAA 208 LYD LR +L++A L + +HD +GH L + + A Sbjct: 152 LYDRLRISEEKLKKANRDLEMYASSVEELTLLRERNRISREIHDSVGHSLSTMVIQLGAI 211 Query: 209 IHTLPHSQETGMEMMNQIRDQLAASMDDMRAAVRRIN--YGSQLEGAYALDRLLEELGRD 266 + + E + +R S+D++RAAVR I + EG +++ L+ + Sbjct: 212 EKIIRKAPEKAELLTKNLRKFTQKSLDEVRAAVRAIKPKEFEEYEGILSIEELISNFKKM 271 Query: 267 TGIETSYQVHGMPYPLYPSVRVTLYKNAQEALTNAIRHGRATAIWINVSFSEQEVAMEVG 326 I+ + L LY+ QE L+N+ RHGRA+ + + ++FSE+ + + + Sbjct: 272 ANIDVRLAFTKEKWSLNSDQSFVLYRIVQEFLSNSARHGRASRVDVIMAFSEKNLLVTLK 331 Query: 327 NNGQRPEENPXXXXXXXXXXXXXXXAERVHLIGGTLELQLEPEFAVITRVALHSQPDKLP 386 +NGQ + ERV +GG E + P ++ L ++ +KL Sbjct: 332 DNGQGCQS-------IIEGVGMKSMRERVKELGGYFEYKTAPYEGFTVKIEL-AKREKLK 383 Query: 387 L 387 + Sbjct: 384 V 384 >CAC2940 [T] COG4585 Signal transduction histidine kinase Length = 402 Score = 68.6 bits (166), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 47/223 (21%), Positives = 98/223 (43%), Gaps = 9/223 (4%) Query: 149 LYDELRKKHFELEEARSSLLQFTXXXXXXXXXXXXXXXXXXLHDDIGHRLIRVKMMTEAA 208 LYD LR+ +L A L +++ +HD +GH L V M +A Sbjct: 165 LYDRLRESEEKLRGAYEKLERYSETVEELSILRERNRISREIHDSVGHTLSAVLMQLQAL 224 Query: 209 IHTLPHSQETGMEMMNQIRDQLAASMDDMRAAVRRINYGS--QLEGAYALDRLLEELGRD 266 ++ + +E ++ + ++ + ++++R VR + + EG +A++ ++ + + Sbjct: 225 LYLIKSDKEESLKTVEEMTGFVRGGIENVRRTVRELKPTDFDRFEGVFAIEEMIRKYKKL 284 Query: 267 TGIETSYQVHGMPYPLYPSVRVTLYKNAQEALTNAIRHGRATAIWINVSFSEQEVAMEVG 326 T + + + L Y+ QEAL NAIRHG+A+ + +++ F++ + + + Sbjct: 285 TSADIRFILSKEKRSLSSDESFISYRVVQEALNNAIRHGKASLVEVSMQFNQDRLYIRIK 344 Query: 327 NNGQRPEENPXXXXXXXXXXXXXXXAERVHLIGGTLELQLEPE 369 +NG + ERV +GG +E E + Sbjct: 345 DNGVGTD-------GIKKGFGMTGMEERVKKLGGNIEFYSERD 380 >BH0784 [T] COG4585 Signal transduction histidine kinase Length = 393 Score = 68.2 bits (165), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 40/143 (27%), Positives = 74/143 (51%), Gaps = 3/143 (2%) Query: 190 LHDDIGHRLIRVKMMTEAAIHTLPHSQ-ETGMEMMNQIRDQLAASMDDMRAAVRRINYG- 247 +HD IGH L + E+ L H + E E + + ++ + + ++R +V+ ++ Sbjct: 191 IHDTIGHTLTTAIVGLESVKRLLQHQRTEEAFEKLTKAQEHIKKGLAEVRRSVKTLHEKP 250 Query: 248 SQLEGAYALDRLLEELGRDTGIETSYQVHGMPYPLYPSVRVTLYKNAQEALTNAIRHGRA 307 S L+ +L+ L +E TG+ + +P L P +TL++ QE LTN IRHG++ Sbjct: 251 SFLQFVPSLEALAKETSESTGVSIHCHIGDLP-KLTPEQELTLFRAFQEGLTNGIRHGQS 309 Query: 308 TAIWINVSFSEQEVAMEVGNNGQ 330 T + + E+ + E+ +NGQ Sbjct: 310 TEFFFTLEAKEERIWFELIDNGQ 332 >CAC1454 [T] COG4585 Signal transduction histidine kinase Length = 441 Score = 59.7 bits (143), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 45/194 (23%), Positives = 90/194 (46%), Gaps = 19/194 (9%) Query: 190 LHDDIGHRLIRVKMMTEAAIHTLPHSQETGMEMMNQIRDQLAASMDDMRAAVRRIN---- 245 +HD +GH L + + +A + T+ S+E + +N I D ++ D+R +V+++ Sbjct: 252 IHDTLGHALTGISVGIDACLTTIDVSKELTKKQLNIIADVAREAIKDVRRSVKKLRPDAL 311 Query: 246 YGSQLEGAYALDRLLEELGRDTGIETSYQVHGMPYPLYPSVRVTLYKNAQEALTNAIRHG 305 S L+ A L+ ++ + + T + + T+Y+ QE +TN+IRHG Sbjct: 312 ENSSLQDA--LNNMISRISKATKTKVFLNANIKLLKFGHDEEDTIYRIIQEGITNSIRHG 369 Query: 306 RATAIWINVSFSEQEVAMEVGNNGQ---RPEENPXXXXXXXXXXXXXXXAERVHLIGGTL 362 +A I+I++ ++++ + + +NG+ EE ERV L+ GT+ Sbjct: 370 QAEEIYIDMIRRDKQLNITIRDNGKGCANVEEG----------FGLRHIEERVKLLNGTV 419 Query: 363 ELQLEPEFAVITRV 376 E F + + Sbjct: 420 EYDGSDSFLITVNI 433 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.137 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,118,545 Number of extensions: 733300 Number of successful extensions: 1604 Number of sequences better than 1.0e-05: 14 Number of HSP's gapped: 1611 Number of HSP's successfully gapped: 14 Length of query: 387 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 279 Effective length of database: 25,922,359 Effective search space: 7232338161 Effective search space used: 7232338161 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 117 (49.7 bits)