BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0118.0007.p (548 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value VNG0816G_2 [G] COG3325 Chitinase 223 8e-58 VCA0027_1 [G] COG3325 Chitinase 206 9e-53 VNG0818C_2 [G] COG3325 Chitinase 184 4e-46 VC1073_2 [G] COG3325 Chitinase 161 3e-39 VCA0700_2 [G] COG3325 Chitinase 155 2e-37 >VNG0816G_2 [G] COG3325 Chitinase Length = 370 Score = 223 bits (567), Expect = 8e-58, Method: Compositional matrix adjust. Identities = 142/403 (35%), Positives = 211/403 (52%), Gaps = 46/403 (11%) Query: 44 KLVGYYASWAAYGRSYNVTDIDPGKMNVINYAFADICWNGVHGNPDPTGPNPVTWTCQNE 103 ++VGYY WA + R Y DI K+ +NYAF + +G V + +N Sbjct: 1 RVVGYYMQWAQWDRDYFPGDIPLDKVTHVNYAFLTVREDGA-----------VDYIQENA 49 Query: 104 QGQTISVPNGTVVLGDPWIDAQKSFGDDK-WDDPIKGNLKQLWKLKEKNPNLKTVISIGG 162 + + KS+ D +DD + +P + SIGG Sbjct: 50 AMRVLE---------------PKSWHDHTGFDDLVD------------DPETSFLFSIGG 82 Query: 163 WTWSNRFSDVAATAATREVFANSAVDFIRKYHMDGVDLDWEYPVSGGLAGNSYRPEDKQN 222 W S FS+ A + A+RE FA++A++ +R ++ DG+D+DWEYP GG +GN R DKQ Sbjct: 83 WNDSTYFSNAAQSQASRERFADTAIEIMRTHNFDGLDIDWEYPGGGGNSGNVVRDGDKQR 142 Query: 223 YVLLLQKIREKLNAAGTADGKTYLLTIASGASPAYVQNNNLSGIAAIVDWINIMTYDFNG 282 Y LLQ +REKL+ A DGK Y LT A A P + + A +D++N+MTYD++G Sbjct: 143 YTELLQTVREKLDVAEDEDGKRYQLTTALSADPEKNTGLDHAANAEALDFLNVMTYDYHG 202 Query: 283 SWNTTTGHNSPLYYDPAAASSGLTDPENFNIDKAVTSYLANGVPANKLVLGLPFYGRGWG 342 ++N T H +PLY A S + + F +D +++ +L +L LGLPFYGR +G Sbjct: 203 AFNDYTNHQAPLYGTEADPSP---NADEFYVDASMSFWLDTAFDPAQLSLGLPFYGRSFG 259 Query: 343 G-APGTGNGQYQVSAGISSTGTWEKGN--YDFWDLEANYINKNGYTRYWNNTAKVPYLYN 399 A NG YQ G + GTW + N ++WD+ N + Y +W++TAKVP+LY+ Sbjct: 260 NVASSDNNGLYQPFDG-TPDGTWGQDNGIKEYWDITQNLAPSSDYETFWDDTAKVPWLYS 318 Query: 400 PTTQTFISYDDAESIGYKTSYIKTKGLAGAMFWETSGDRNKTL 442 P+ +SYD S+ KT+Y + G MFW SGD+N+ L Sbjct: 319 PSKNVLVSYDSPRSVEAKTAYAAQHDIGGMMFWTFSGDKNEVL 361 >VCA0027_1 [G] COG3325 Chitinase Length = 594 Score = 206 bits (523), Expect = 9e-53, Method: Compositional matrix adjust. Identities = 143/427 (33%), Positives = 202/427 (47%), Gaps = 42/427 (9%) Query: 48 YYASWAAYGRSYNVTDIDPGKMNVINYAFADICWNGVHGNPDPTGPNPVTWTCQNEQGQT 107 Y+ W+ Y R + V +I + I Y F IC G + + G N + Q Sbjct: 164 YFVEWSIYDRKFTVDNIPGQNLTHILYGFIPIC--GPNESLKSVGGN----SFNALQTAC 217 Query: 108 ISVPNGTVVLGDPWIDAQKSF--GDDKWDDPIKGNLKQLWKLKEKNPNLKTVISIGGWTW 165 VP+ VV+ DPW QKSF ++ PIKGN L LK+ P+LK + SIGGWT Sbjct: 218 KGVPDFEVVIHDPWAAYQKSFPQAGHQYSSPIKGNYAMLMALKKTYPDLKIIPSIGGWTL 277 Query: 166 SNRFSDVAATAATREVFANSAVDFIRKYHM-DGVDLDWEYPVSGGLAGNSYRP-EDKQNY 223 S+ F A R+VF S F++ + DGVD+DWEYP GG A + P +D Y Sbjct: 278 SDPFFSFT-DKAKRDVFVASVKRFLKTWKFYDGVDIDWEYPGGGGQAADLGDPVKDGPAY 336 Query: 224 VLLLQKIREKLNAAGTADGKTYLLTIASGASPAYVQNNNLSGIAAIVDWINIMTYDFNGS 283 V L+ ++R L+ G+ Y LT A G +++ N +D+I MTYDF G Sbjct: 337 VALMAELRAMLDELEAETGRKYELTSAIGVGHDKIEDVNYGQAVQYMDYIFAMTYDFYGG 396 Query: 284 WNTTTGHNSPLY---YDPAAASSGLTDPEN--------FNIDKAVTSYLANGVPANKLVL 332 WN GH + LY + G EN + D + LA GVP +KLV+ Sbjct: 397 WNNVLGHQTALYCGSFMRPGQCDGKGVDENGEPYKGPAYTTDNGIQLLLAQGVPPSKLVV 456 Query: 333 GLPFYGRGWGGA------------PGTGNGQYQVSAGISSTGTWEKGNYDF-----WDLE 375 G YGRGW G G GNG+ + G ++ G WE G D+ + L Sbjct: 457 GAAMYGRGWEGVTPASLKDPNDPMTGVGNGKLK---GTTAQGVWEAGVIDYKGVKNFMLG 513 Query: 376 ANYINKNGYTRYWNNTAKVPYLYNPTTQTFISYDDAESIGYKTSYIKTKGLAGAMFWETS 435 AN NG+ ++ A+ P+++N TT +++DD S+ K +Y++ GLAG WE Sbjct: 514 ANKTGVNGFEYGYDEQAEAPWVWNRTTGQLVTFDDDRSVKAKGAYVRNLGLAGLFSWEID 573 Query: 436 GDRNKTL 442 D L Sbjct: 574 ADNGDIL 580 >VNG0818C_2 [G] COG3325 Chitinase Length = 403 Score = 184 bits (466), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 140/442 (31%), Positives = 201/442 (45%), Gaps = 83/442 (18%) Query: 43 YKLVGYYASWAAYGR-SYNVTDIDPGKMNVINYAFADICWNGVHGNPDPTGPNPVTWTCQ 101 +K+VGYY SW + D+ ++ + YAF D+ Sbjct: 4 FKVVGYYPSWKGTDDYDFYPADVPFDQVTDVLYAFLDVQ--------------------- 42 Query: 102 NEQGQTISVPNGTVVLGDPWIDAQKSFGDDKWDDPIKGNLKQLWKLKEKNPNLKTVISIG 161 P+GTVVL D +D + +LKQ ++ + + +SIG Sbjct: 43 ---------PDGTVVLPDSDVDHESLLAS-------FASLKQ-----DRAADTRLKLSIG 81 Query: 162 GWTWSNRFSDVAATAATREVFANSAVDFIRKYHMDGVDLDWEYPVSGGLAGNSYRPEDKQ 221 GW S F D AA A+RE FA +AVD +R Y DG+D+DWE+P + Sbjct: 82 GWGLSPGFEDAAADQASRERFATTAVDLMRTYDFDGIDVDWEHPGPRRGKCECGSAQGPA 141 Query: 222 NYVLLLQKIREKLNAAGTADGKTYLLTIASGASPAYVQNNNLSGIAAIVDWINIMTYDFN 281 N+V LL+ +R++LN A DG+TY L++A+G S N +AA+VD I +M YDF Sbjct: 142 NHVALLETVRDRLNDAEVEDGRTYDLSVANGGSDWNAALINHREVAAVVDDIYMMAYDFT 201 Query: 282 GSWNTTTGHNSPLY-YDPAAASSGLTDPENFNIDKAVT------------SYLANGVPAN 328 G W+ T G N+P+Y P SG D + + ++ +T + +G P + Sbjct: 202 GVWHGTAGLNAPIYGTPPDYPPSG--DAQQYTLETTLTIWKEQGYWVDWMEWEDHGDPVD 259 Query: 329 K---LVLGLPFYGRGWGGAPG-----------TGNGQYQVSAGISSTGTWEK-------- 366 LVLG+PFYGRG G G+ +YQ + GTW Sbjct: 260 DPGTLVLGMPFYGRGCNVENGIWDTFSLSEWQQGDPKYQND--VIPPGTWNDLRGPDDAN 317 Query: 367 -GNYDFWDLEANYINKNGYTRYWNNTAKVPYLYNPTTQTFISYDDAESIGYKTSYIKTKG 425 G +D+ DL ANY + +T+ N VPYL+N + FISYDD SI K + Sbjct: 318 TGAFDYGDLAANYEGADAWTKQRNEQGGVPYLWNDSEGVFISYDDPTSIAAKVELAVAED 377 Query: 426 LAGAMFWETSGDRNKTLQTKLN 447 L G M WE S D + TL +N Sbjct: 378 LGGVMIWELSQDYDGTLLETIN 399 >VC1073_2 [G] COG3325 Chitinase Length = 446 Score = 161 bits (407), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 131/473 (27%), Positives = 213/473 (45%), Gaps = 107/473 (22%) Query: 45 LVGYYASWAAYGR----SYNVTDIDPGKMNVINYAFADICWNGVHGNPDPTGPNPVTWTC 100 ++GYY SW G+ +Y D+ K+ INYAFA I + D + +TW Sbjct: 1 IIGYYTSWRT-GKNGLPAYLAGDLPWEKLTHINYAFASINKSDFSMQVDDSA-TKMTWE- 57 Query: 101 QNEQGQTISVPNGTVVLGDPWIDAQKSFGDDKWDDPIKGNLKQLWKLKEKNPNLKTVISI 160 +VP + DP + P +G+ L K K++ P++KT+IS+ Sbjct: 58 --------NVPGAEM---DPSL-------------PYQGHFNLLSKFKKQYPDVKTLISV 93 Query: 161 GGWTWSNRF----SDVAATAATRE---VFANSAVDFIRKYHMDGVDLDWEYPVSGGLAGN 213 GGW + F +D+A+ + E F SAVDFIR+Y DGVD+D+EYP S +GN Sbjct: 94 GGWAETGGFYPMTTDLASCSVNMEGIKAFNKSAVDFIRQYDFDGVDIDYEYPSSMKDSGN 153 Query: 214 SYRPEDK--------QNYVLLLQKIREKLNAAGTADGKTYLLTIASGASPAYVQNNNLSG 265 E NY++++ ++R+ L+ AG DG+ Y+LTIAS +S ++ Sbjct: 154 PVDFEQSNKCRGQLWDNYMVMMTELRKALDKAGEEDGRRYMLTIASPSSAYLLRGMQDFA 213 Query: 266 IAAIVDWINIMTYDFNGSWNTTTGHNSPLY---YDPAAASSGLTDPENFN----IDKAVT 318 + ++D++NIM+YD +G+WN G + L+ D A G+ + +++A T Sbjct: 214 MQDVLDYVNIMSYDLHGTWNEFVGPQAALFDDGKDAELAKWGVYTTAEYQGIGYLNQAWT 273 Query: 319 SYLANGV-PANKLVLGLPFYGRGWGGAPGTGNG---------QYQVSAGISSTGTWEKGN 368 + G +++ +G+P+Y RGW G G G Q G + G +G Sbjct: 274 HHFFRGAFKPSQINMGIPYYTRGWQGVSGGDKGLWGRAVEPNQSSCPEGTTVCGWGAEGT 333 Query: 369 YDFW-DLEAN-------------------------------------------YINKNGY 384 + W D++AN ++ + Y Sbjct: 334 DNIWHDVDANGNEIKAGVVPMWHAMNLMHAEKLGIDGMPSYGPAWGMDPNNPKHLIEGKY 393 Query: 385 TRYWNNTAKVPYLYNPTTQTFISYDDAESIGYKTSYIKTKGLAGAMFWETSGD 437 R W+ + +L+N T + F+S +D +S+ K YI GL G M WE +GD Sbjct: 394 ERVWSQELQTAWLWNDTKKVFLSIEDKDSLKPKLDYIVDNGLGGMMIWEMAGD 446 >VCA0700_2 [G] COG3325 Chitinase Length = 474 Score = 155 bits (391), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 143/486 (29%), Positives = 209/486 (43%), Gaps = 121/486 (24%) Query: 44 KLVGYYASWAAYG---RSYNVTDIDPGKMNVINYAFADICWNGVHGNPDPTGPNPVTWTC 100 +++GY+ SW + +Y V DI ++ INYAF I +G D PN Sbjct: 18 RVIGYFTSWRSGDDPQAAYLVKDIPWEQLTHINYAFVSIGSDGKVNVGDVNDPN------ 71 Query: 101 QNEQGQTISVPNGTVVLGDPWIDAQKSFGDDKWDDPIKGNLKQLWKLKEKNPNLKTVISI 160 N V P ++ + G KG+ L K+K +KT+ISI Sbjct: 72 -----------NAAVGKEWPGVEIDPTLG-------FKGHFGALATYKQKY-GVKTLISI 112 Query: 161 GGWTWS--------NRFSDV-----------AATAATREVFANSAVDFIRKYHMDGVDLD 201 GGW + NR +D + A E FA SAV+ IRKY DGVD+D Sbjct: 113 GGWAETGGHFDANGNRVADGGFYTMTTNADGSINHAAIEKFAASAVEMIRKYKFDGVDID 172 Query: 202 WEYPVSGGLAGNSYRPEDK-----------QNYVLLLQKIREKLNAAGTADGKTYLLTIA 250 +EYP S AGN P+DK +Y L++ +REKL+ A DG Y+LTIA Sbjct: 173 YEYPTSMAGAGN---PDDKGFMEPRRAYLWASYQELMRVLREKLDQASAQDGHHYMLTIA 229 Query: 251 SGASPAYVQNNNLSGIAAIVDWINIMTYDFNGSWNTTTGHNSPLY---YDPAAASSGLTD 307 + +S ++ + +D++NIM+YD +G+WN GHN+ LY D A + Sbjct: 230 APSSGYLLRGMETFDVTKYLDYVNIMSYDLHGAWNDHVGHNAALYDTGKDSELAQWNVYG 289 Query: 308 PENF------NIDKAVTSYLANGVPANKLVLGLPFYGRGWGG------------------ 343 + N D A Y +PA ++ +G+P+Y RGW G Sbjct: 290 TAQYGGIGYLNTDWAY-HYFRGSMPAGRINIGVPYYTRGWQGVTGGENGLWGRAALPNQN 348 Query: 344 --APGTGNGQ---------------YQVSA-----GISSTGTWEKGNYD--FWDLEANYI 379 APGTG G+ + V+A G S W N + W A Sbjct: 349 LCAPGTGEGEKNNCGHGATGIDNMWHDVNAAGDEMGAGSNPMWHAKNLEKGIWGSYAAAY 408 Query: 380 NKN--------GYTRYWNNTAKVPYLYNPTTQTFISYDDAESIGYKTSYIKTKGLAGAMF 431 + Y R +++ A P+L+N + F+S +D +SI K Y+ K + G MF Sbjct: 409 KLDPTTTPLIGTYVRNYDSVAVAPWLWNAEKKVFLSTEDKQSIDVKADYVIDKEIGGIMF 468 Query: 432 WETSGD 437 WE +GD Sbjct: 469 WELAGD 474 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.134 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 41,030,232 Number of extensions: 1783429 Number of successful extensions: 2969 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 2952 Number of HSP's successfully gapped: 8 Length of query: 548 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 437 Effective length of database: 25,576,357 Effective search space: 11176868009 Effective search space used: 11176868009 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 118 (50.1 bits)