BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0117.0008.p
         (267 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DR0599 [V] COG2746 Aminoglycoside N3'-acetyltransferase               272   3e-73
DR2034 [V] COG2746 Aminoglycoside N3'-acetyltransferase                72   8e-13

>DR0599 [V] COG2746 Aminoglycoside N3'-acetyltransferase
          Length = 279

 Score =  272 bits (696), Expect = 3e-73,   Method: Compositional matrix adjust.
 Identities = 143/251 (56%), Positives = 177/251 (70%), Gaps = 6/251 (2%)

Query: 10  TVESLAADFRKLGVQEGMTLLLHSSFKSLGQWVAGGPVAVILALEQVLGDEGTLVMPTQS 69
           T  SLAAD R LGVQ G  LL+HSS   LG WVAGG VAVI AL+ VL  +GTLVMPT S
Sbjct: 21  TRASLAADLRSLGVQAGDVLLVHSSLSRLG-WVAGGAVAVIQALQDVLTPQGTLVMPTHS 79

Query: 70  SDLTDPSGWSNPPVPEAWWQGIREHMPAYDPDLTLLRGMGVIPETFRKQKGVKRSSHPIS 129
           SDLTDP+GW +PPVPEAWW+ IR  +PA+DP LT  RGMG IPE FR   GV+RS HP S
Sbjct: 80  SDLTDPAGWLSPPVPEAWWEVIRAELPAFDPALTPTRGMGRIPELFRTWPGVRRSDHPHS 139

Query: 130 SFAAWGRHRDEIIDGHALEYAFGEQSPLARIYDLKGSVLLLGIDSLSNTSLHLAEYRAEY 189
           SFAAWGRH + +  GHAL ++ GE SPLARIYDL G VLLLG +   NT+LHLAE RA  
Sbjct: 140 SFAAWGRHAEFVTAGHALAFSLGEGSPLARIYDLNGRVLLLGTE--RNTNLHLAEVRAGV 197

Query: 190 AGKQEVTAGAPMLVDGARQWVEFRDYNWNSDDFAAIGRDFGQETGQITYGKVAAAEAQLI 249
             +  V    P++VDG RQW+ F + +++++ FA +   F +  G +  G V +A+A+L+
Sbjct: 198 --RPTVAVSGPVMVDGERQWLTFDEPDYDAEAFAPVKAAF-EAAGGVVRGTVGSAQARLM 254

Query: 250 PQREIVDYAVK 260
            QRE+VD+AV 
Sbjct: 255 SQRELVDFAVN 265


>DR2034 [V] COG2746 Aminoglycoside N3'-acetyltransferase
          Length = 258

 Score = 72.0 bits (175), Expect = 8e-13,   Method: Compositional matrix adjust.
 Identities = 69/260 (26%), Positives = 113/260 (43%), Gaps = 41/260 (15%)

Query: 9   ITVESLAADFRKLGVQEGMTLLLHSSFKSLGQWVAGGPVAVILALEQVLGDEGTLVMP-- 66
           +T   L      LG+     +++H+S +S GQ + GG   V+  LE+      TLV P  
Sbjct: 1   MTAADLDRGLDSLGLDGTQNVIVHASLRSFGQ-LEGGARTVVDELER---RTSTLVAPAF 56

Query: 67  TQSSDLTDPSGWSNPPVPEAWWQGIREHMPAYDPDLTLLRGMGVIPETFRKQKGVKRSSH 126
           T ++ L+ PS     PV             +++ D  + R +G +P+   ++   +RS H
Sbjct: 57  TYATLLSRPSS----PV-----------RASFNRDSRVSRDIGRVPQEMVERAAARRSFH 101

Query: 127 PISSFAAWGRHRDEIIDGHALEYAFGEQSPLARIYDLKGSVLLLGIDSLSNTSLHLAEYR 186
           P  SF A G+  + +     L+  +    P+  +YDL G  LL+G+D  SNT++H  E+ 
Sbjct: 102 PALSFVALGQEAERVTAAQTLDSPY---QPIGALYDLDGYALLMGVDFGSNTTVHYGEH- 157

Query: 187 AEYAGKQEVTAGAPMLVDGARQWVEFRDYNWNSDDFAAIGRDFGQETGQI--TYGKVAAA 244
                     AG P+L     +WV   D   +   F     DF      +     +V  A
Sbjct: 158 ---------LAGMPLLT----RWVPL-DGQVSPTAFPNCSADFANLEPYVRGREVQVGPA 203

Query: 245 EAQLIPQREIVDYAVKWLEQ 264
             +L   R++VD  V+ L  
Sbjct: 204 TLRLYRVRDLVDETVRLLSH 223


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.317    0.135    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 21,863,012
Number of extensions: 948746
Number of successful extensions: 1535
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 1529
Number of HSP's successfully gapped: 2
Length of query: 267
Length of database: 38,378,431
Length adjustment: 105
Effective length of query: 162
Effective length of database: 26,268,361
Effective search space: 4255474482
Effective search space used: 4255474482
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 115 (48.9 bits)