BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0117.0003.p (1848 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yfkN_2 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodieste... 327 1e-88 mlr3017_1 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodieste... 306 3e-82 AGl2132 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiestera... 287 1e-76 SMc04018 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiester... 286 2e-76 DRA0018 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiestera... 279 4e-74 >BS_yfkN_2 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 481 Score = 327 bits (837), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 204/511 (39%), Positives = 290/511 (56%), Gaps = 66/511 (12%) Query: 865 FNLRVLHTNDTHGHLENITKRTSAISSER--TGNAVLLDAGDVFSGTLYFTQFKGQADIE 922 ++L V+HTNDTH HL++ +R + I+ R T + +LLDAGDVFSG LYFT++ G AD++ Sbjct: 16 WDLTVMHTNDTHAHLDDAARRMTKINEVRSETNHNILLDAGDVFSGDLYFTKWNGLADLK 75 Query: 923 FMNNIGYDAMTFGNHEFDLNAKEPEVLKNFVT---------------AAKFPFTSANIDF 967 MN +GYDAMTFGNHEFD K P VL +F++ A +FP SAN+D Sbjct: 76 MMNMMGYDAMTFGNHEFD---KGPTVLSDFLSGNSATVDPANRYHFEAPEFPIVSANVDV 132 Query: 968 TTKGSELAELYHDAIGMLETDETKSTAKDGHIYPSVIKDVYGEKVGIFGLTTEDTVGLSS 1027 + + +L T K+ I+P ++ DV GEKV +FGLTTEDT SS Sbjct: 133 SNE-PKLKSFVKKPQTF-----TAGEKKEAGIHPYILLDVDGEKVAVFGLTTEDTATTSS 186 Query: 1028 PGEQISFKDHVESAKKTV-AMLEAQGINKIIAVTHLGYTVDQELAKAVPGIDIIVGGHSH 1086 PG+ I F D E+A+ TV A+ E + +NKIIA+TH+G+ D ELAK V GID+I+GGH+H Sbjct: 187 PGKSIVFNDAFETAQNTVKAIQEEEKVNKIIALTHIGHNRDLELAKKVKGIDLIIGGHTH 246 Query: 1087 TKIDNPPAPIVNAGTGKNVLIVQTGEYSQFLGELNVTFDKDGEIL--AYNGKLLDVNLFG 1144 T +D +VN + ++ Q EY QFLG ++V FD+ G + N +L ++ Sbjct: 247 TLVD--KMEVVN--NEEPTIVAQAKEYGQFLGRVDVAFDEKGVVQTDKSNLSVLPIDEHT 302 Query: 1145 EDS-TAKKLLAPYDAKLAIVRNTVVGYSDVDLYTNRMIDGKSVRVVRQEETPIGNMIADS 1203 E++ AK+ L + +L V+N VGY+DV L DG+ VR +ET +GN IAD Sbjct: 303 EENPEAKQELDQFKNELEDVKNEKVGYTDVAL------DGQREH-VRTKETNLGNFIADG 355 Query: 1204 IAEKVRELMPNFVSASDLASIKGVVAIQNGGGIRAAIDKGDITMGEVLTTLPFGNSLAAL 1263 + K +E + +AI NGGGIRA IDKGDIT+GEVL +PFGN+L Sbjct: 356 MLAKAKE------------AAGARIAITNGGGIRAGIDKGDITLGEVLNVMPFGNTLYVA 403 Query: 1264 KVTGAEIIXXXXXXXXXXXXDQGRFAQVSGMKYTYDSTKKPEIVDSLTGQVTQAGERIVS 1323 +TG +I G F QV+G++YT+ KP G R++ Sbjct: 404 DLTGKQIKEALEQGLSNVENGGGAFPQVAGIEYTFTLNNKP-------------GHRVLE 450 Query: 1324 VEIRQTDGSYLPIDPNAYYLLSTNSFMAGGG 1354 V+I +G + I+ + Y ++TN+F+ GG Sbjct: 451 VKIESPNGDKVAINTDDTYRVATNNFVGAGG 481 >mlr3017_1 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 656 Score = 306 bits (783), Expect = 3e-82, Method: Compositional matrix adjust. Identities = 200/576 (34%), Positives = 305/576 (52%), Gaps = 103/576 (17%) Query: 864 NFNLRVLHTNDTHGHLENITKRTS---------------------AISSERTG----NAV 898 ++ L +LH ND H +E K S AI+ ER N + Sbjct: 25 DYTLNILHFNDWHSRIEGNNKYESTCSADEETKGECIGGAGRLITAIAQERKKLEGQNVL 84 Query: 899 LLDAGDVFSGTLYFTQFKGQADIEFMNNIGYDAMTFGNHEFDLNAKEPEVLKNFVTAAKF 958 LL+AGD F G+L++T +KG + EF+N + DA+T GNHEFD L ++ AKF Sbjct: 85 LLNAGDSFQGSLFYTTYKGTVEEEFLNQMKPDAVTLGNHEFD---DGESALVPYLDKAKF 141 Query: 959 PFTSANIDFTTKGSELAELYHDAIGMLETDETKSTAKDGHIYPSVIKDVYGEKVGIFGLT 1018 P SAN+ + D++ + G I PS++ +V G+K+GI G Sbjct: 142 PIVSANV-------------------MPNDKSGAA---GKIKPSIVVEVGGQKIGIVGAV 179 Query: 1019 TEDTVGLSSPGEQISFKDHVESAKKTVAMLEAQGINKIIAVTHLGYTVDQELAKAVPGID 1078 T DT L+SPG I+ D VES V L+AQG+NKIIAVTH+GY ++++ +PG+D Sbjct: 180 TNDTPELASPGPNIAIADDVESITAEVEKLKAQGVNKIIAVTHIGYNRERDIIAKIPGVD 239 Query: 1079 IIVGGHSHTKIDN-------PPAPIVNAGTGKNVLIVQTGEYSQFLGELNVTFDKDGEIL 1131 ++VGGHSHT + N P +V+ G V +VQ YS++LGE V FD +G + Sbjct: 240 VVVGGHSHTLLSNTDPKAAGPYPTMVDNPGGYKVPVVQAASYSKYLGEFKVVFDDNGVVK 299 Query: 1132 AYNGK--LLDVNLFGEDST---AKKLLAPYDAKLAIVRNTVVGYSDVDLYTNRMIDGKSV 1186 + +G LD ++ + + K+L AP +A ++N V T +IDG S Sbjct: 300 SASGDPIFLDKSITPDPAVLARIKELGAPIEA----LKNKEVA------ETTDVIDG-SR 348 Query: 1187 RVVRQEETPIGNMIADSIAEKVRELMPNFVSASDLASIKGV-VAIQNGGGIRAAIDKGDI 1245 R +E +GN+++D+I ++V+ +GV + I NGGG+RA+IDKG + Sbjct: 349 ENCRAKECEMGNLVSDAILDRVKG--------------QGVEIVISNGGGLRASIDKGTV 394 Query: 1246 TMGEVLTTLPFGNSLAALKVTGAEIIXXXXXXXXXXXXDQGRFAQVSGMKYTYDSTKKPE 1305 TMGEVLT LPF N+LA +++G +++ GRF QV+G+KY++D + P Sbjct: 395 TMGEVLTVLPFQNTLATFQISGKDLVAGLESGLSQIEDGAGRFPQVAGLKYSFDKSVAP- 453 Query: 1306 IVDSLTGQVTQAGERIVSVEIRQTDGSYLPIDPNAYYLLSTNSFMAGGGDFYRALAGAKA 1365 AG R+ SVE+ + +G++ PI P+ YL++TN+++ GGD Y+ A Sbjct: 454 ----------NAG-RVKSVEVME-NGAWTPIKPDKDYLVATNNYVRQGGDGYKVFAERAK 501 Query: 1366 DGRYYELGLPDFEVLLSYLKLHHPINSKVEGRITDL 1401 + Y GL +V+ YL H P K++GRIT++ Sbjct: 502 NAYDYGPGLE--QVVADYLGAHRPYTPKLDGRITEI 535 >AGl2132 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 60 Score = 287 bits (734), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 0/579 (0%), Positives = 0/579 (0%), Gaps = 108/579 (18%) Query: 864 NFNLRVLHTNDTHGHLENITKRTSAISSERTG---------------------------N 896 + ++ + R Sbjct: 21 PELVDLVMATPLVHAGAVTKTLGVTPQAARRI 80 Query: 897 AVLLDAGDVFSGTLYFTQFKGQADIEFMNNIGYDAMTFGNHEFDLNAKEPEVLKNFVTAA 956 Sbjct: 81 --- 137 Query: 957 KFPFTSANIDFTTKGSELAELYHDAIGMLETDETKSTAKDGHIYPSVIKDVYGEKVGIFG 1016 Sbjct: 138 ---------------------- 175 Query: 1017 LTTEDTVGLSSPGEQISFKDHVESAKKTVAMLEAQGINKIIAVTHLGYTVDQELAKAVPG 1076 Sbjct: 176 235 Query: 1077 IDIIVGGHSHTKIDN-------PPAPIVNAGTGKNVLIVQTGEYSQFLGELNVTFDKDGE 1129 Sbjct: 236 295 Query: 1130 ILAYNGK--LLDVNLFGEDSTAKK---LLAPYDAKLAIVRNTVVGYSDVDLYTNRMIDGK 1184 Sbjct: 296 --------- 346 Query: 1185 SVRVVRQEETPIGNMIADSIAEKVRELMPNFVSASDLASIKGV-VAIQNGGGIRAAIDKG 1243 Sbjct: 347 ---------------- 390 Query: 1244 DITMGEVLTTLPFGNSLAALKVTGAEIIXXXXXXXXXXXXDQGRFAQVSGMKYTYDSTKK 1303 Sbjct: 391 450 Query: 1304 PEIVDSLTGQVTQAGERIVSVEIRQTDGSYLPIDPNAYYLLSTNSFMAGGGDFYRALAGA 1363 Sbjct: 451 -------------- 496 Query: 1364 KADGRYYELGLPDFE-VLLSYLKLHHPINSKVEGRITDL 1401 Sbjct: 497 --- 532 >SMc04018 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 628 Score = 286 bits (733), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 192/576 (33%), Positives = 292/576 (50%), Gaps = 104/576 (18%) Query: 864 NFNLRVLHTNDTHGHLENITKRTSAISSERTG-------------------------NAV 898 ++ L +LH ND H +E+I K S S+E G N + Sbjct: 25 DYELNILHINDLHSRIESINKFDSTCSAEEEGKNECFGGVARLKTLIDQKRQELTGKNVL 84 Query: 899 LLDAGDVFSGTLYFTQFKGQADIEFMNNIGYDAMTFGNHEFDLNAKEPEVLKNFVTAAKF 958 LL+AGD F G+L+FT +KG + EF+N + +DAMT GNHEFD + + L +F+ F Sbjct: 85 LLNAGDNFQGSLFFTTYKGATEAEFLNLMKFDAMTVGNHEFD---EAEDGLASFLDKVAF 141 Query: 959 PFTSANIDFTTKGSELAELYHDAIGMLETDETKSTAKDGHIYPSVIKDVYGEKVGIFGLT 1018 P +AN+ + S++ + I PS++ DV G+K+GI G Sbjct: 142 PVVTANV-LPSHKSKIGD---------------------RIKPSIVLDVGGQKIGIVGAV 179 Query: 1019 TEDTVGLSSPGEQISFKDHVESAKKTVAMLEAQGINKIIAVTHLGYTVDQELAKAVPGID 1078 DT LSS G I + V + + ++ QG+NKIIA+TH+GY D +P +D Sbjct: 180 ANDTPELSSVGPDILIGEDVATITSAIEEVKKQGVNKIIALTHVGYPRDLAAIAKIPDVD 239 Query: 1079 IIVGGHSHTKIDN-------PPAPIVNAGTGKNVLIVQTGEYSQFLGELNVTFDKDGEIL 1131 ++VGGHSH+ + N P +V+ G V +VQ G YS++LG+L VTFD G + Sbjct: 240 VVVGGHSHSLLSNTDEKAEGPYPTMVDNPGGYKVPVVQAGSYSKYLGDLVVTFDDSGVVK 299 Query: 1132 AYNGK--LLDVNLFGEDST---AKKLLAPYDAKLAIVRNTVVGYSDVDLYTNRMIDGKSV 1186 A G L+D ++ +++ K+L P + +R+ V+ T IDG S Sbjct: 300 AAKGDPILVDSSVKPDEAVVARVKELAKPIEE----LRSKVIA------KTEAPIDG-SR 348 Query: 1187 RVVRQEETPIGNMIADSIAEKVRELMPNFVSASDLASIKGV-VAIQNGGGIRAAIDKGDI 1245 R +E +G+++AD++ ++V+ +GV VAI NGGG+RA+ID GD+ Sbjct: 349 ETCRAKECEMGSLVADAMLDRVKG--------------QGVTVAITNGGGLRASIDGGDV 394 Query: 1246 TMGEVLTTLPFGNSLAALKVTGAEIIXXXXXXXXXXXXDQGRFAQVSGMKYTYDSTKKPE 1305 TMGEV+T LPF N+L+ ++ G++I GRF QV+G+KY++D +K Sbjct: 395 TMGEVITVLPFQNTLSTFQLKGSDIRAALENGLSQVEEGGGRFPQVAGLKYSFDRSKP-- 452 Query: 1306 IVDSLTGQVTQAGERIVSVEIRQTDGSYLPIDPNAYYLLSTNSFMAGGGDFYRALAGAKA 1365 AG R+VSVE+++ D ++ +DP Y L +N+FM GGGD Y Sbjct: 453 -----------AGSRLVSVEVKEGD-AFAALDPEKTYSLVSNNFMRGGGDGYAVFKDKGE 500 Query: 1366 DGRYYELGLPDFEVLLSYLKLHHPINSKVEGRITDL 1401 + Y GL VL YL H P +GRI ++ Sbjct: 501 NAYDYGPGLE--TVLADYLAAHQPFKPYTDGRIAEV 534 >DRA0018 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 524 Score = 279 bits (713), Expect = 4e-74, Method: Compositional matrix adjust. Identities = 191/555 (34%), Positives = 277/555 (49%), Gaps = 92/555 (16%) Query: 869 VLHTNDTHGHLE--NITKRTSAISSERTG----------NAVLLDAGDVFSGTLYFTQFK 916 +LHT+D HGHLE + + T S +T N ++L GD F GTL+F ++ Sbjct: 29 ILHTDDLHGHLEPTKVGQNTYGGYSRQTTLVKQFTASDPNPLVLSGGDTFQGTLFFNLYQ 88 Query: 917 GQADIEFMNNIGYDAMTFGNHEFDLNAKEPEVLKNFVTAAKFPFTSANIDFTTKGSELAE 976 G AD+ FMN Y AM GNHEFD P L F A FP +AN+D +T+ Sbjct: 89 GLADVLFMNYQAYQAMAVGNHEFD---NGPAALARFAQKANFPLLAANLDVSTE-----P 140 Query: 977 LYHDAIGMLETDETKSTAKDGHIYPSVIKDVYGEKVGIFGLTTEDTVGLSSPGEQISFKD 1036 L D + P + V GEK+G+ G T D +SSPG + + Sbjct: 141 LLKDLV-----------------KPYAVLSVGGEKIGVIGAVTPDLPTISSPGPNVKMLE 183 Query: 1037 HVESAKKTVAMLEAQGINKIIAVTHLGYTVDQELAKAVPGIDIIVGGHSHTKI------D 1090 + S K + L AQGINKI+ V+HLGYTV+Q++A VPGID+IVGGHSHT + D Sbjct: 184 LMTSLKNSADQLRAQGINKIVLVSHLGYTVEQQVAATVPGIDVIVGGHSHTLLGTFDNKD 243 Query: 1091 NPPAP-----IVNAGTGKNVLIVQTGEYSQFLGELNVTFDKDGEILAYNGKLLDVNL-FG 1144 PP+ +V G L+V E+ + LG L VTFD G + A+ G + V Sbjct: 244 FPPSEGPYPTVVQNPDGNKTLLVAAWEWGKVLGRLKVTFDDAGAVTAWEGNPIPVTSDIA 303 Query: 1145 EDSTAKKLLAPYDAKLAIVRNTVVGYSDVDLYTNRMIDGKSVRVVRQEETPIGNMIADSI 1204 ED TA++++ +A + G T +DG S VVR+ E+ + N++AD+ Sbjct: 304 EDDTARRMIGTLSVPIANRASRSWG------TTASALDG-SREVVRRRESTMANVLADA- 355 Query: 1205 AEKVRELMPNFVSASDLASIKGVVAIQNGGGIRAAIDKGDITMGEVLTTLPFGNSLAALK 1264 L+ + ++LA + NGGG+RA+ID+G +T E +T PFGN+L + Sbjct: 356 -----ALLAGQNAGAELALV-------NGGGVRASIDQGPVTFEEAITVQPFGNTLTVIT 403 Query: 1265 VTGAEIIXXXXXXXXXXXXDQGRFAQVS-GMKYTYDSTKKPEIVDSLTGQVTQAGERIVS 1323 +TGA++ ++G+F VS GM YT+D TK AG R+ + Sbjct: 404 LTGAQLKAALEYGVATWSENKGQFLHVSRGMSYTFDPTKP-------------AGSRVSA 450 Query: 1324 VEIRQTDGSYLPIDPNAYYLLSTNSFMAGGGDFYRALAGAKADGRYYELGLPDFEVLLSY 1383 V + P+D Y ++TN+F A GGD + D E G D +VL++Y Sbjct: 451 VTLNGQ-----PLDDARAYKVATNNFTASGGDGFTMF----KDAPKLETGTLDVDVLVNY 501 Query: 1384 LKLHHPINSKVEGRI 1398 LK + +N++ EGRI Sbjct: 502 LKANPTLNAQPEGRI 516 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.131 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 126,858,881 Number of extensions: 5474023 Number of successful extensions: 9611 Number of sequences better than 1.0e-05: 50 Number of HSP's gapped: 9441 Number of HSP's successfully gapped: 56 Length of query: 1848 Length of database: 38,378,431 Length adjustment: 121 Effective length of query: 1727 Effective length of database: 24,423,017 Effective search space: 42178550359 Effective search space used: 42178550359 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 123 (52.0 bits)