BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0117.0002.p (431 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0021 [J] COG0172 Seryl-tRNA synthetase 256 5e-68 STM1379 [E] COG0531 Amino acid transporters 245 1e-64 AGc3129 [J] COG0172 Seryl-tRNA synthetase 238 1e-62 CAC0017 [J] COG0172 Seryl-tRNA synthetase 216 5e-56 SPAC29A4.15 [J] COG0172 Seryl-tRNA synthetase 205 1e-52 >CAC0021 [J] COG0172 Seryl-tRNA synthetase Length = 346 Score = 256 bits (654), Expect = 5e-68, Method: Compositional matrix adjust. Identities = 0/424 (0%), Positives = 0/424 (0%), Gaps = 13/424 (3%) Query: 1 MLDMNWIRDNEELMRNTAVWKKVDFPL---EDLLEWDDKXXXXXXXXXXXXXXXNGLTKE 57 M + E+ + + D E Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 58 VERLLRQXXXXXXXXXXXQVREINAHLTKLEADLTEAEQRCSELLLLAPNPVSADTPIGA 117 + L RE +K+ ++ L + Sbjct: 61 TQGL--TETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSL 118 Query: 118 DDSGNVELRRNGTLPDFGFTARDHVELGELHSIIDIPRGVKAGGPRSYVLKGAGLYLHLA 177 L T + + + + Sbjct: 119 KRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGV 178 Query: 178 VQRLALDVLAER-GFTVMDVPVIVRPEALERTGFFPGGMDQTYEL-NGEKRWLAGTSEVS 235 A V+ D+P IV + + Sbjct: 179 SNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYE--GVELLAYSCL 236 Query: 236 LVSLYSDEIVEAE-VPLRLAGMSACFRREVGSAGRDVRGLYRVHQFSKIEQVVICKNDPA 294 + + + + + F R G + Y A Sbjct: 237 GFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMA--LA 294 Query: 295 ESEQMLQEILANAEHILQLLELPYRVVAVCSGDMALKTHKQYDIETWMPSRGAYGETHSA 354 Sbjct: 295 354 Query: 355 SNLLDFQARRSGIRF-RDKDGRLQYCHTLNNTAVATPRILIPLLENHQQEDGSIYIPKAL 413 Sbjct: 355 414 Query: 414 RKYM 417 Sbjct: 415 418 >STM1379 [E] COG0531 Amino acid transporters Length = 447 Score = 245 bits (625), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 28/421 (6%), Positives = 74/421 (17%), Gaps = 8/421 (1%) Query: 1 MLDMNWIRDNEELMRNTAVWKKVDFP--LEDLLEWDDKXXXXXXXXXXXXXXXNGLTKEV 58 M + + + ++ V+ P L ++ Sbjct: 1 MKKVKKLSLTDLVLYG-LVFMVPIAPVSLYGVVYNLSHGMVALVYIIGAIAMFFTAYSYS 59 Query: 59 ERLLRQXXXXXXXXXXXQVREINAHLTKLEADLTEAEQRCSELLLLAPNPVSADTPIGAD 118 L + + + +L V A P Sbjct: 60 TLSQHISSSGSAYAYAGVCINPAVGFLTGWILLLDYLLFPTLVAVLGGVAVHAILPQIPV 119 Query: 119 DSGNVELRRNGTLPDFGFTARDHVELGELHSIIDIPRGVKAGGPRSYVLKGAGLYLHLAV 178 + GT ++ + L I + +L + Sbjct: 120 WVWPLIYVAIGTGVNYLGIQQTAKFDKLLVFIQLGILAIFVLLIVRLMLLDSSQITLSFR 179 Query: 179 QRLALDV-LAERGFTVMDVPVIVRPEALERTGFFPGGMDQTYELNGEKRWLAGTSEVSLV 237 T + V + + ++ + V + Sbjct: 180 PFFDSQWFTPGLIATAISVAALNFLGFDAISTLSEESEGGGRAVSKATLLALVLATVLFI 239 Query: 238 SLYSDEIVE-AEVPLRLAGMSACFRREVGSAGRDVRGLYRVHQFSKIEQVVICKNDPAES 296 + + V G + + L + + Sbjct: 240 IVVAFAAFATGNVDRFAEGNATNEAFFTIAGNVGGIWLKVAFSIIVAFVCAVGN--IITA 297 Query: 297 EQMLQEILANAEHILQLLELPYRVVAVCSGDMALKTHKQYDIETWMPSRGAYGETHSASN 356 + + +L + L V E+ S Sbjct: 298 QTAVSRVLFSMGRDRMLPAFLAHVHTTRKTPDYAILFTGGVTLLLSYLFSGKIESISTLV 357 Query: 357 LLDFQA-RRSGIRFRDKDGRLQYCHTLNNTAVATPRILIPLLENHQQEDGSIYIPKALRK 415 + A I+ ++ + + +I+ Sbjct: 358 NFGALFAFFVVNLCVFILFNFRMKAQRRIFAHVISPIMGMIVIGYVCLNMNIHALILGIS 417 Query: 416 Y 416 + Sbjct: 418 W 418 >AGc3129 [J] COG0172 Seryl-tRNA synthetase Length = 346 Score = 238 bits (608), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 0/422 (0%), Positives = 0/422 (0%), Gaps = 6/422 (1%) Query: 1 MLDMNWIRDNEELMRNTAVWKKVDFPLEDLLEWDDKXXXXXXXXXXXXXXXNGLTKEVER 60 + + D Sbjct: 9 SSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETY 68 Query: 61 LLRQXXXXXXXXXXXQVREINAHLTKLEADLTEAEQRCSELLLLAPNPVSADTPIGADDS 120 + +++ + + + + + A + D Sbjct: 69 VGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDL 128 Query: 121 GNVELRRNGTLPDFGFTARDHVELGELHSIIDIPRGVKAGGPRSYVLKGAGLYLHLAVQR 180 V + T + + + + G V R Sbjct: 129 YQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMR 188 Query: 181 LALDV-LAERGFTVMDVPVIVRPEALERTGFFPGGMDQTYELNGEKRWLAGTSEVSLVSL 239 + V G + EL L + Sbjct: 189 YLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELL-AYSCLGFGTLTGKYLN 247 Query: 240 YSDEIVEAE-VPLRLAGMSACFRREVGSAGRDVRGLYRVHQFSKIEQVVICKNDPAESEQ 298 + + R S ++ +A D+ + + V + A + Sbjct: 248 GAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLL 307 Query: 299 MLQEILANAEHILQLLELPYRVVAVCSGDMALKTHKQYDIETWMPSRGAYGETHSASNLL 358 Sbjct: 308 -- 365 Query: 359 DFQARRSGIRFRDKDGR-LQYCHTLNNTAVATPRILIPLLENHQQEDGSIYIPKALRKYM 417 Sbjct: 366 425 Query: 418 DG 419 Sbjct: 426 427 >CAC0017 [J] COG0172 Seryl-tRNA synthetase Length = 346 Score = 216 bits (550), Expect = 5e-56, Method: Compositional matrix adjust. Identities = 0/425 (0%), Positives = 0/425 (0%), Gaps = 13/425 (3%) Query: 1 MLDMNWIRDNEELMRNTAVWKKVD-FPLEDLLEWDDKXXXXXXXXXXXXXXXNGLTKEVE 59 M + E+ + + Sbjct: 1 MQYHRIPHSSLEVSTL-GLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRP 59 Query: 60 RLLRQXXXXXXXXXXXQVREINAHLTKLEADLTEAEQRCSELLLLAPNPVSADTPIGADD 119 + + + A + + + Sbjct: 60 ETQGLTETYVGNWLA-KHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSL 118 Query: 120 SGNVELRRNGTLPDFGFTARDHVELGELHSIIDIPRGVKAGGPRSYVLKGAGLYLHLAVQ 179 + +S D V + + + Sbjct: 119 KRLQ-TDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIG 177 Query: 180 RLALDVLAERGFTVMDVPVIVRPEALERTGFFPGGMDQTYEL--NGE---KRWLAGTSEV 234 + + + + + L + LA + Sbjct: 178 VSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLG 237 Query: 235 SLVSLYSDEIV-EAEVPLRLAGMSACFRREVGSAGRDVRGLYRVHQFSKIEQVVICKNDP 293 + + + + + Sbjct: 238 FGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALA--F 295 Query: 294 AESEQMLQEILANAEHILQLLELPYRVVAVCSGDMALKTHKQYDIETWMPSRGAYGETHS 353 Sbjct: 296 355 Query: 354 ASNLLDFQARRSGIRF-RDKDGRLQYCHTLNNTAVATPRILIPLLENHQQEDGSIYIPKA 412 Sbjct: 356 415 Query: 413 LRKYM 417 Sbjct: 416 420 >SPAC29A4.15 [J] COG0172 Seryl-tRNA synthetase Length = 346 Score = 205 bits (521), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 0/355 (0%), Positives = 0/355 (0%), Gaps = 19/355 (5%) Query: 84 LTKLEADLTEAEQ-RCSELLLLAP---NPVSADTPIGADDSGNVELRR---NGTLPDFGF 136 + + + + + + ++L + + Sbjct: 83 IIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFG 142 Query: 137 TARDHVELGELHSIIDIPRGVKAGGPRSYVLKGAGLYLHLAVQRLALDVLAERGFTVMDV 196 + + + + + + + Sbjct: 143 KLGYSWTDSAPAVS-LLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRI 201 Query: 197 PVIVRPEALERTGFFPGGMDQTYEL--NGEKRWLAGTSEVSLVSLYSDEIVEA---EVPL 251 I P +L F G + + G ++ L + A Sbjct: 202 VTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSR 261 Query: 252 RLAGMSACFRREVGSAGRDVRGLYRVHQFSKIEQVVICKNDPAESEQMLQEILANAEHIL 311 ++ V + R + V S + + + + Sbjct: 262 FTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRR--QPFVASTLLGATTMDQLKTNI 319 Query: 312 QLLELPYRVVAVCSGDMALKTHKQYDIETWMPSRGAYGETHSASNLLDFQARRSGIRFRD 371 Sbjct: 320 379 Query: 372 K---DGRLQYCHTLNNTAVATPRILIPLLENHQQEDGSIYIPKALRKYMDGQEFL 423 Sbjct: 380 - 433 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,337,492 Number of extensions: 1310972 Number of successful extensions: 11553 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 11518 Number of HSP's successfully gapped: 8 Length of query: 431 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 322 Effective length of database: 25,807,025 Effective search space: 8309862050 Effective search space used: 8309862050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)