BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0109.0027.p (324 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3408 [O] COG0492 Thioredoxin reductase 451 e-127 BS_yumC [O] COG0492 Thioredoxin reductase 418 e-117 lin2489 [O] COG0492 Thioredoxin reductase 407 e-114 SP1563 [O] COG0492 Thioredoxin reductase 322 4e-88 BS_ycgT [O] COG0492 Thioredoxin reductase 299 3e-81 >BH3408 [O] COG0492 Thioredoxin reductase Length = 346 Score = 451 bits (1161), Expect = e-127, Method: Compositional matrix adjust. Identities = 18/323 (5%), Positives = 59/323 (18%), Gaps = 1/323 (0%) Query: 1 MSDLLIIGGGPAGMFAAFYGGMRQASVTLIESMPQLGGQLAALYPEKYIYDVAGFPKVTG 60 L + G + ++ G L + + +T Sbjct: 7 PHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTE 66 Query: 61 QELVDNLSRQMELFQSDIRLEEKVVSLKKQDERHFVITTDKAEYHSKAVIITAGVGAFEP 120 + + L++ + I + S D+ + Sbjct: 67 TYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYL 126 Query: 121 RRLELPEAARFEKANLHYFVSDLNAFKGKKVLISGGGDSAVDWALMLEPIAEQVTLIHRR 180 ++ R S ++ +L + + A + I Sbjct: 127 DLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGV 186 Query: 181 DKFRAHEHSVENLMASKVNVITPSEITGLHGE-EFITKVTLSHIKTKETQELEVDSVIVN 239 ++ + + +++ + + Sbjct: 187 MRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYL 246 Query: 240 FGFVSSLGPIAEWGIDIESNSIVVDSRMETSIPGIFAAGDITTYPGKLKLIAVGFGEAPT 299 G + + + + + G + F + Sbjct: 247 NGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTL 306 Query: 300 AVNNAKVYIDPEAKLSPGHSSNL 322 + + S Sbjct: 307 LGATTMDQLKTNIESLHLELSED 329 >BS_yumC [O] COG0492 Thioredoxin reductase Length = 346 Score = 418 bits (1075), Expect = e-117, Method: Compositional matrix adjust. Identities = 21/320 (6%), Positives = 56/320 (17%) Query: 3 DLLIIGGGPAGMFAAFYGGMRQASVTLIESMPQLGGQLAALYPEKYIYDVAGFPKVTGQE 62 L + G + ++ G L + + +T Sbjct: 9 SSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETY 68 Query: 63 LVDNLSRQMELFQSDIRLEEKVVSLKKQDERHFVITTDKAEYHSKAVIITAGVGAFEPRR 122 + + L++ + I + S D+ + Sbjct: 69 VGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDL 128 Query: 123 LELPEAARFEKANLHYFVSDLNAFKGKKVLISGGGDSAVDWALMLEPIAEQVTLIHRRDK 182 ++ R S ++ +L + + A + I + Sbjct: 129 YQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMR 188 Query: 183 FRAHEHSVENLMASKVNVITPSEITGLHGEEFITKVTLSHIKTKETQELEVDSVIVNFGF 242 + + + + Sbjct: 189 YLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNG 248 Query: 243 VSSLGPIAEWGIDIESNSIVVDSRMETSIPGIFAAGDITTYPGKLKLIAVGFGEAPTAVN 302 G S + + I + L + A T + Sbjct: 249 AKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLG 308 Query: 303 NAKVYIDPEAKLSPGHSSNL 322 + S + Sbjct: 309 ATTMDQLKTNIESLHLELSE 328 >lin2489 [O] COG0492 Thioredoxin reductase Length = 346 Score = 407 bits (1047), Expect = e-114, Method: Compositional matrix adjust. Identities = 19/323 (5%), Positives = 60/323 (18%), Gaps = 5/323 (1%) Query: 3 DLLIIGGGPAGMFAAFYGGMRQASVTLIESMPQLGGQLAALYPEKYIYDVAGFPKVTGQE 62 L + G + ++ G L + + +T Sbjct: 9 SSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETY 68 Query: 63 LVDNLSRQMELFQSDIRLEEKVVSLKKQDERHFVITTDKAEYHSKAVIITAGVGAFEPRR 122 + + L++ + I + S D+ + Sbjct: 69 VGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDL 128 Query: 123 LELPEAARFEKANLHYFVSDLNAFKGKKVLISGGGDSAVDWALMLEPIAEQVTLIHRRDK 182 ++ R S ++ +L + + A + I + Sbjct: 129 YQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMR 188 Query: 183 FRAHEHSVENLMASKVNVIT---PSEITGLHGEEFITKVTLSHIKTKETQELEVDSVIVN 239 + + + E +++ + + Sbjct: 189 YLHLADKHDLPRIVTIQNPYSLLNRSFEV-GLAE-VSQYEGVELLAYSCLGFGTLTGKYL 246 Query: 240 FGFVSSLGPIAEWGIDIESNSIVVDSRMETSIPGIFAAGDITTYPGKLKLIAVGFGEAPT 299 G + + + + + G + F + Sbjct: 247 NGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTL 306 Query: 300 AVNNAKVYIDPEAKLSPGHSSNL 322 + + S Sbjct: 307 LGATTMDQLKTNIESLHLELSED 329 >SP1563 [O] COG0492 Thioredoxin reductase Length = 346 Score = 322 bits (826), Expect = 4e-88, Method: Compositional matrix adjust. Identities = 29/323 (8%), Positives = 55/323 (17%), Gaps = 6/323 (1%) Query: 1 MSDLLIIGGGPAGMFAAFYGGMRQASVTLIESMPQLGGQLAALYPEKYIYDVAGFPKVTG 60 G + + + Q L +Y V P+ G Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 61 QELVDNLSRQMELFQSDIRLEEKVVSLKKQDERHFVITTDKAEYHSKAVIITAGVGAFEP 120 + + + + VS ++ K + Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNN--DKGIRPDQALDRKNIREALHDSLKRL 121 Query: 121 RRLELPEAARFEKANLHYFVSDLNAFKGKKVLISGGGDSAVDWALMLEPIAEQVTLIHRR 180 + L AL A Sbjct: 122 QTDYLDLYQVHW--PQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAG-KIRYIGV 178 Query: 181 DKFRAHEHSVENLMASKVNVITPSEITGLHGE-EFITKVTLSHIKTKETQELEVDSVIVN 239 A +A K ++ I + +V L+ + E EL S + Sbjct: 179 SNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGF 238 Query: 240 FGFVSSLGPIAEWGIDIESNSIVVDSRMETSIPGIFAAGDITTYPGKLKLIAVGFGEAPT 299 A+ + AA L + Sbjct: 239 GTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRR 298 Query: 300 AVNNAKVYIDPEAKLSPGHSSNL 322 A + + Sbjct: 299 QPFVASTLLGATTMDQLKTNIES 321 >BS_ycgT [O] COG0492 Thioredoxin reductase Length = 346 Score = 299 bits (766), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 19/323 (5%), Positives = 53/323 (16%), Gaps = 2/323 (0%) Query: 3 DLLIIGGGPAGMFAAFYGGMRQASVTLIESMPQLGGQLAALYPEKYIYDVAGFPKVTGQE 62 L + G + ++ G L + + +T Sbjct: 9 SSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETY 68 Query: 63 LVDNLSRQMELFQSDIRLEEKVVSLKKQDERHFVITTDKAEYH-SKAVIITAGVGAFEPR 121 + + L++ + I + S D+ + + + Sbjct: 69 VGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDL 128 Query: 122 RLELPEAARFEKANLHYFVSDLNAFKGKKVLISGGGDSAVDWALMLEPIAEQVTLIHRRD 181 + +A + + T Sbjct: 129 YQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMR 188 Query: 182 KFRAHEHSVENLMASKVNVITPSEITGLHG-EEFITKVTLSHIKTKETQELEVDSVIVNF 240 + + + N + + G E + + + +N Sbjct: 189 YLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNG 248 Query: 241 GFVSSLGPIAEWGIDIESNSIVVDSRMETSIPGIFAAGDITTYPGKLKLIAVGFGEAPTA 300 + S + D Sbjct: 249 AKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLG 308 Query: 301 VNNAKVYIDPEAKLSPGHSSNLK 323 L S ++ Sbjct: 309 ATTMDQLKTNIESLHLELSEDVL 331 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,335,453 Number of extensions: 955931 Number of successful extensions: 4597 Number of sequences better than 1.0e-05: 101 Number of HSP's gapped: 4494 Number of HSP's successfully gapped: 101 Length of query: 324 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 217 Effective length of database: 26,037,693 Effective search space: 5650179381 Effective search space used: 5650179381 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)