BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0109.0021.p (368 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3411 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and relate... 487 e-137 aq_648 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and relate... 343 3e-94 DR0366 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and relate... 340 3e-93 Cj1368 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and relate... 272 6e-73 AF0388 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and relate... 266 5e-71 >BH3411 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes Length = 364 Score = 487 bits (1253), Expect = e-137, Method: Compositional matrix adjust. Identities = 220/359 (61%), Positives = 295/359 (82%) Query: 9 TDARMANIIDKVRGGERLNLEDGVYLYGSNDLLTIGQLANEVNQRKNGNKVYFIENMSLY 68 TD ++ I +KV GERL +EDG+YLY ++DLL + QLAN+VN KNG+ VYFIENM + Sbjct: 6 TDTKLQEIQEKVEHGERLTIEDGLYLYETSDLLGVAQLANKVNSEKNGDHVYFIENMYIN 65 Query: 69 FTNVCEAHCAFCNFRKDDGEEGAYTLSGQEMVQYVEQHIHPGVREFHIVGGHNDKVPFQY 128 TNVCEA+C+FC F++ G+EGAYT+ + +++YVE+ + +REFHIVGGHN VPF Y Sbjct: 66 PTNVCEANCSFCGFKRKPGQEGAYTMDSETLLRYVEERWNDNIREFHIVGGHNTDVPFDY 125 Query: 129 YVDSLKALNEAFPQVTLKAYTAAEIDFFTRISGLSIREVLEELRAAGLQSLTGGGAEILS 188 Y+D+++ L + FPQVT+KAYT AEI+FF+R++G+S++EVLEEL AGL +L GGGAEIL+ Sbjct: 126 YLDTIRTLKKNFPQVTVKAYTGAEIEFFSRLAGISMKEVLEELIKAGLDTLPGGGAEILT 185 Query: 189 DQYRKKMRVNKANVEEYLEVHRTAHQLGMKTHTTMLYGSIESHEDRVRHMLQIRDLQDET 248 ++YR KM +KA+ +++LE H AH LG+KTH TMLYGSIES+E+R+ HM ++R LQD+T Sbjct: 186 ERYRAKMSPDKASTDQWLEAHEIAHGLGLKTHATMLYGSIESNEERLIHMDRVRQLQDKT 245 Query: 249 NGFMVFIPLSMQPKNKNAGIMRRNSAYEDLKTIAISRLMLDNFDHIKAYFINIGPQLTQV 308 NG+MVFIPL+MQP++ AG+ RR SA++D++TIAISRLMLDN DHIKAY+INIG QLTQ+ Sbjct: 246 NGYMVFIPLAMQPRSVKAGLKRRTSAFDDMRTIAISRLMLDNIDHIKAYWINIGVQLTQM 305 Query: 309 ALSFGASDVHGTILKERISHAAGALTPEGLTREELIWLVKGAGKIPVERDTFYNEIKVY 367 AL+FG+ D+HGT+++ERISH+ GALT +G+TR+ELI L+K A K PVERDTFYN IK Y Sbjct: 306 ALTFGSDDIHGTLIEERISHSVGALTSQGITRDELIHLIKSANKTPVERDTFYNIIKSY 364 >aq_648 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes Length = 371 Score = 343 bits (880), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 176/368 (47%), Positives = 241/368 (65%), Gaps = 1/368 (0%) Query: 1 MSTLITPQTDARMANIIDKVRGGERLNLEDGVYLYGSNDLLTIGQLANEVNQRKNGNKVY 60 + T+ D I DKV +R+N E+ +YLY SN+L +G LA +N++KNG Y Sbjct: 5 VETVFQTVRDKEFLRIADKVLNEKRINQEEALYLYRSNELTALGLLAEYINRKKNGMYAY 64 Query: 61 FIENMSLYFTNVCEAHCAFCNFRKDDGEEGAYTLSGQEMVQYVEQHIHPGVREFHIVGGH 120 FI N + TNVC C FC F + AY +S +E+++ VE+ G RE HIVGG Sbjct: 65 FIVNRQINPTNVCMYQCDFCAFGVKKSDPKAYEMSMEEILKKVEELYLMGGREVHIVGGI 124 Query: 121 NDKVPFQYYVDSLKALNEAFPQVTLKAYTAAEIDFFTRISGLSIREVLEELRAAGLQSLT 180 + YV ++ + + FP++ +KA+TA EI +ISG S EVL EL+ AGL +L Sbjct: 125 PSYWDYDKYVSIVREIKKRFPEIIIKAWTAVEIHHMAKISGKSYEEVLSELKEAGLDALP 184 Query: 181 GGGAEILSDQYRKKMRVNKANVEEYLEVHRTAHQLGMKTHTTMLYGSIESHEDRVRHMLQ 240 GGGAEI S++ RK + KAN EEYLEVHR AH+LG+ ++ TMLYG +E+ E+RV HM Sbjct: 185 GGGAEIFSERVRKIIAPYKANAEEYLEVHRVAHKLGIPSNATMLYGHVETLEERVEHMEM 244 Query: 241 IRDLQDETNGFMVFIPLSMQPKNKNAGIMRRNSAYEDLKTIAISRLMLDNFDHIKAYFIN 300 +R LQDET GF VFIPL+ P+ G R S+ +DLKTIAISR+ LDNFDHIKAY++ Sbjct: 245 LRRLQDETGGFQVFIPLAYWPEGTRLG-GTRTSSVDDLKTIAISRIYLDNFDHIKAYWVT 303 Query: 301 IGPQLTQVALSFGASDVHGTILKERISHAAGALTPEGLTREELIWLVKGAGKIPVERDTF 360 +G ++ QVAL+FGA D+ GTI +E+I HAAG + G + ++L+ L+K AGK P ERDTF Sbjct: 304 LGEKVAQVALNFGADDLDGTIQEEKIVHAAGTKSAYGHSVDKLVNLIKKAGKTPAERDTF 363 Query: 361 YNEIKVYE 368 Y ++VY+ Sbjct: 364 YRVVRVYD 371 >DR0366 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes Length = 376 Score = 340 bits (871), Expect = 3e-93, Method: Compositional matrix adjust. Identities = 158/362 (43%), Positives = 247/362 (68%), Gaps = 4/362 (1%) Query: 10 DARMANIIDKVRGGERLNLEDGVYLYGSNDLLTIGQLANEVNQRKNGNKVYFIENMSLYF 69 D +A I++KV G+RL ++G+ L+ + DL T+ +LA+ ++ +G+KV+F+ +M L F Sbjct: 6 DPGLAPIVEKVEAGQRLTFDEGMQLFHTRDLNTLMRLADLQKRQLHGDKVFFVHSMRLEF 65 Query: 70 TNVCEAHCAFCNFRKDDGEEGAYTLSGQEMVQYVEQHIHPGVREFHIVGGHNDKVPFQYY 129 TN+C C FC F GEE A+ E+V V + PG+ E H+ GH+ ++YY Sbjct: 66 TNICYVGCTFCAFAARKGEERAWDYDPDEVVAQVGRRYLPGITELHMSSGHHPNHKWEYY 125 Query: 130 VDSLKALNEAFPQVTLKAYTAAEIDFFTRISGLSIREVLEELRAAGLQSLTGGGAEILSD 189 ++ L EA+P + +KA+TAAEI+ ++IS REVL EL+AAGL ++ GGGAEI +D Sbjct: 126 PAMVQKLREAYPDLQVKAFTAAEIEHLSKISKKPTREVLAELQAAGLSAMPGGGAEIFAD 185 Query: 190 QYRKKMRVNKANVEEYLEVHRTAHQLGMKTHTTMLYGSIESHEDRVRHMLQIRDLQDETN 249 + RK++ NK E++L++HR AH+LGM+T+ TMLYG IE+ E+R+ HM ++R+LQD+T Sbjct: 186 RVRKQVAKNKVKAEKWLQIHREAHELGMRTNATMLYGHIETLEERLDHMHRLRELQDQTG 245 Query: 250 GFMVFIPLSMQPKN----KNAGIMRRNSAYEDLKTIAISRLMLDNFDHIKAYFINIGPQL 305 GF FIPL+ QP +N G + +DL+ +A++R+ LDNF HIK Y++ IG +L Sbjct: 246 GFHAFIPLAFQPLGNTLAQNLGKTEYTTGLDDLRNLAVARIYLDNFPHIKGYWVMIGSEL 305 Query: 306 TQVALSFGASDVHGTILKERISHAAGALTPEGLTREELIWLVKGAGKIPVERDTFYNEIK 365 TQV+L +G SD+ GTI +E I+HAAGA +P L++ ++ +++ AG++PV RD +YNE++ Sbjct: 306 TQVSLDWGVSDIDGTIQEEHIAHAAGATSPMALSQAGMVKMIQSAGRVPVLRDAYYNELE 365 Query: 366 VY 367 V+ Sbjct: 366 VF 367 >Cj1368 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes Length = 354 Score = 272 bits (696), Expect = 6e-73, Method: Compositional matrix adjust. Identities = 142/355 (40%), Positives = 212/355 (59%), Gaps = 1/355 (0%) Query: 13 MANIIDKVRGGERLNLEDGVYLYGSNDLLTIGQLANEVNQRKNGNKVYFIENMSLYFTNV 72 M N+I K+ ERLN E+ L+ DL T+G+ A++ + +G KVYF N + TN+ Sbjct: 1 MKNLITKLENEERLNQEEANALWDL-DLFTLGKYAHKKRTKLHGKKVYFNINRHINPTNI 59 Query: 73 CEAHCAFCNFRKDDGEEGAYTLSGQEMVQYVEQHIHPGVREFHIVGGHNDKVPFQYYVDS 132 C C FC F Y ++ +E+++ V++ + +E HIV HN +Q+Y++ Sbjct: 60 CADTCKFCAFSAHRKNPNPYLMTHEEIMKIVDETVKRETKEVHIVSAHNKDTTWQWYLEI 119 Query: 133 LKALNEAFPQVTLKAYTAAEIDFFTRISGLSIREVLEELRAAGLQSLTGGGAEILSDQYR 192 K + E +P + +KA TAAEIDF R +S EV+E++ G+ S+ GGGAEI ++ R Sbjct: 120 FKMIKEKYPNLHVKAMTAAEIDFLHRRFKMSYEEVIEKMLEYGVDSMPGGGAEIFDEEVR 179 Query: 193 KKMRVNKANVEEYLEVHRTAHQLGMKTHTTMLYGSIESHEDRVRHMLQIRDLQDETNGFM 252 KK+ K + E +L++H+ H+ +++ TML+G IE R+ HML++RDLQD+T GF Sbjct: 180 KKICHGKVSSENWLKIHKLWHEKSKQSNATMLFGHIEERHHRIDHMLRLRDLQDQTGGFN 239 Query: 253 VFIPLSMQPKNKNAGIMRRNSAYEDLKTIAISRLMLDNFDHIKAYFINIGPQLTQVALSF 312 FIPL Q + + E LKTIAISRL+LDN +IKAY+ + L VA F Sbjct: 240 AFIPLVWQRDKSFIQTDKIMDSEEILKTIAISRLVLDNIKNIKAYWATMTLNLAMVAQEF 299 Query: 313 GASDVHGTILKERISHAAGALTPEGLTREELIWLVKGAGKIPVERDTFYNEIKVY 367 GA+D+ GTI KE I A GA + +G + + I ++K + IPVERD+ YNE+K Y Sbjct: 300 GANDLDGTIEKESIQSAGGAKSAKGTSLKTFIDMIKTSNLIPVERDSLYNELKTY 354 >AF0388 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes Length = 306 Score = 266 bits (679), Expect = 5e-71, Method: Compositional matrix adjust. Identities = 130/309 (42%), Positives = 203/309 (65%), Gaps = 8/309 (2%) Query: 61 FIENMSLYFTNVCEAHCAFCNFRKDDGEEGAYTLSGQEMVQYVEQHIHPGVREFHIVGGH 120 F+ N + +T++C A C C F +G Y LS +E+++ V + G+ E HIVGGH Sbjct: 3 FVVNRHINYTDICVAKCPLCAFNNREG----YLLSPEEILRKVREV--DGITEVHIVGGH 56 Query: 121 NDKVPFQYYVDSLKALNEAFPQVTLKAYTAAEIDFFTRISGLSIREVLEELRAAGLQSLT 180 N +V +Y+ + + + P+V +KA TA E+ ++ + +SIRE L L+ AGLQ++ Sbjct: 57 NPEVGVEYFEEVFRRIKREKPEVVIKALTATEVYYYAKKEKMSIREFLSRLKDAGLQAMP 116 Query: 181 GGGAEILSDQYRKKMRVNKANVEEYLEVHRTAHQLGMKTHTTMLYGSIESHEDRVRHMLQ 240 GGGAEIL D R+K+ NK + +E+L V + AH+LG+K++ TML+G +E R H+ + Sbjct: 117 GGGAEILVDSVRRKISPNKCSSDEWLRVMKIAHELGIKSNATMLFGHVEEIRHRAIHLYR 176 Query: 241 IRDLQDETNGFMVFIPLSMQPKNKNAG--IMRRNSAYEDLKTIAISRLMLDNFDHIKAYF 298 +R LQ++T GF+ FIPL P+N G + + + + LKTIA+SR+ LDNF ++AY+ Sbjct: 177 LRKLQEKTGGFVSFIPLVYHPENNPLGKIVREKTNPVDILKTIAVSRIALDNFRSVRAYW 236 Query: 299 INIGPQLTQVALSFGASDVHGTILKERISHAAGALTPEGLTREELIWLVKGAGKIPVERD 358 + +G +LT+VAL FGA+D+ GT+++E+++HAAGA TP GLT E L+ + + AGK +RD Sbjct: 237 VMLGEKLTEVALRFGANDIDGTVVEEKVTHAAGAETPAGLTVERLVEICRSAGKRVGQRD 296 Query: 359 TFYNEIKVY 367 TF N +K Y Sbjct: 297 TFCNILKWY 305 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,568,220 Number of extensions: 1125910 Number of successful extensions: 2254 Number of sequences better than 1.0e-05: 39 Number of HSP's gapped: 2179 Number of HSP's successfully gapped: 39 Length of query: 368 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 260 Effective length of database: 25,922,359 Effective search space: 6739813340 Effective search space used: 6739813340 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)