BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0109.0010.p (542 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC3213 [R] COG2244 Membrane protein involved in the export of O... 145 1e-34 BH3277 [R] COG2244 Membrane protein involved in the export of O-... 144 5e-34 BS_yabM [R] COG2244 Membrane protein involved in the export of O... 128 2e-29 SA1575 [R] COG2244 Membrane protein involved in the export of O-... 126 8e-29 lin0247 [R] COG2244 Membrane protein involved in the export of O... 120 4e-27 >CAC3213 [R] COG2244 Membrane protein involved in the export of O-antigen and teichoic acid Length = 432 Score = 145 bits (367), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 0/455 (0%), Positives = 0/455 (0%), Gaps = 20/455 (4%) Query: 4 KKESFVKGTXXXXXXXXXXXXXXXXQRVPLEHLFNDIGNASFTQANNVYLMLLPLATAGI 63 + + V + ++ G Sbjct: 2 QVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGFRKGKVPMNIVAQRY-GA 60 Query: 64 PSTLSKMVSERYALNRPHEAQQVYRAALIFAAVVGVLMSTILYIAAPYYAEYSKVPESTX 123 + ++ A V G + + + Y +V Sbjct: 61 SVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGEDFTYSVEFEVYPEVELQGL 120 Query: 124 XXXXXXXXX-XXFPMIAMMRGYFQGRNNMMAGGISQIIEQIARVSTAILLAFILLRQGYS 182 + +E RV+ + Sbjct: 121 EAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEAEDRVTIDFTGSVDGEEFEGG 180 Query: 183 NTWMAAGASFGSVLGSIGAFGVMLYYAVKLRRSEEQKALYDSSQARIPLLKIYKDIFKLS 242 + + EE + K + Sbjct: 181 --KASDFVLAMGQGRM-IPGFEDGI--KGHKAGEEFTIDVTFPEEY----HAENLKGKAA 231 Query: 243 IPIVLSSVTVPVVNFIDTSFIVP--LLSGQIGREAATQALGIFGSRAQSVAGIPPVLSIA 300 + + E + R A V S A Sbjct: 232 KFAINLKKVEERELPEL-TAEFIKRFGVEDGSVEGLRAEVRKNMERELKSAIRNRVKSQA 290 Query: 301 LSTSLIPVISAAYARREEAHLKRQITLALRISILTGTPIVLSLVVAAYSVNGLLF-SSLD 359 + + A ++ + A + + L Sbjct: 291 IEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPRELFEEQAKRRVVVGLL 350 Query: 360 GSGIVAMLTLGTIFQITMMTTNSILLGMGKARISMYYV-LAGLLVKFGSNFLLS-RLFGI 417 ++ L + + + + + L+ N L + Sbjct: 351 LGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNKELMDNMRNVALEEQAVEA 410 Query: 418 YGIIAATALCFIVITLLNLRMLKTIVPFEILGKRW 452 Sbjct: 411 --- 442 >BH3277 [R] COG2244 Membrane protein involved in the export of O-antigen and teichoic acid Length = 432 Score = 144 bits (362), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 0/434 (0%), Positives = 0/434 (0%), Gaps = 14/434 (3%) Query: 31 VPLEHLFNDIGNASFTQANNVYLMLLPLATAGIPSTLSKMVSERYALNRPHEAQQVYRAA 90 + V + ++ +++ A Sbjct: 29 KSELVNVAKKVRIDGFRKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAG 88 Query: 91 LIFAAVVGVLMSTILYIAAPYYA-------EYSKVPESTXXXXXXXXXXXXFPMIAMMRG 143 + + + + + Sbjct: 89 APTYVPGEYKLGEDFTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQ 148 Query: 144 YFQGRNNMMAGGISQIIEQIARVSTAILLAFILLRQGYSNTWMAAG-ASFGSVLGSIGAF 202 + ++ F + A Sbjct: 149 ATWKEKDGAVEAEDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAG 208 Query: 203 GVMLYYAVKLRRSEEQKALYDSSQARIPLLKIYKDIFKLSIPIVLSSVTVPVVNFIDTSF 262 + +++ I L K+ + + V + Sbjct: 209 EEFTIDVTFPEEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRA 268 Query: 263 IVPLLSGQIGREAATQALGIFGSRAQSVAGIPPVLSIALSTSLIPVISAAYARREEAHLK 322 V + + A + A V + + + + + A R + Sbjct: 269 EVRKNMERELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQ 328 Query: 323 RQITLALRISILTGTPIVLSLVVAAYSVNG-LLFSSLDGSGIVAMLTLGTI-FQITMMTT 380 +V+ L++ L G++ ++ Sbjct: 329 ALELPRELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLI-EEMASAYEDPKEVIEF 387 Query: 381 NSILL-GMGKARISMYYVLAGLLVKFGSNFLLSRLFGIYGIIAATALCFIVITLLNLRML 439 S Sbjct: 388 YSKNKE 447 Query: 440 KTI--VPFEILGKR 451 Sbjct: 448 461 >BS_yabM [R] COG2244 Membrane protein involved in the export of O-antigen and teichoic acid Length = 432 Score = 128 bits (322), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 23/390 (5%), Positives = 62/390 (15%), Gaps = 8/390 (2%) Query: 30 RVPLEHLFNDIGNASFTQANNVYLMLLPLATAGIPSTLSKMVSERYALNRPHEAQQVYRA 89 V + + + + +S+ + + A Sbjct: 34 NVAKKVRIDGFRKGKVPMNIVAQRYGASVRQDVLGDLMSRNFID-AIIKEKIN---PAGA 89 Query: 90 ALIFAAVVGVLMSTILYIAAPYYAEYSKVPESTXXXXXXXXXXXXFPMIAMMRGYFQGRN 149 + + Y E + M+ + + Sbjct: 90 PTYVPGEYKLGEDFTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQA 149 Query: 150 NMMAGGISQIIEQIARVSTAILLAFILLRQGYSNTWMAAGASFGSVLGSIGAFGVMLYYA 209 + E + + G ++ ++ A + G Sbjct: 150 TWKEKDGAVEAEDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGE 209 Query: 210 VKLRRSEEQKALYDSSQARIPLLKIYKDIFKLSIPIVLSSVTVPVVNFIDTSFIVPLLSG 269 + + + I + + Sbjct: 210 EFTIDVTFPE---EYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGL 266 Query: 270 QIGREAATQALGIFGSRAQSVAGIPPVLSIALSTSLIPVISAAYARREEAHLKRQITLAL 329 + + + V + + +P Q Sbjct: 267 RAEVRKNMERELKS-AIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGN 325 Query: 330 RISILTGTPIVLSLVVAAYSVNGLLFSSLDGSGIVAMLTLGTIFQITMMTTNSILLGMGK 389 L + V GLL + + + I M + Sbjct: 326 EKQALELPRELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVI 385 Query: 390 ARISMYYVLAGLLVKFGSNFLLSRLFGIYG 419 S L + Sbjct: 386 EFYSKNKELMDNMRNVALEEQAVEAVLAKA 415 >SA1575 [R] COG2244 Membrane protein involved in the export of O-antigen and teichoic acid Length = 432 Score = 126 bits (317), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 0/450 (0%), Positives = 0/450 (0%), Gaps = 44/450 (9%) Query: 31 VPLEHLFNDIGN-ASFTQANNVYLMLLPLATAGIPSTLSKMVSERYALNRPHEAQQVYRA 89 L ++ + + ++ + L ++S + E A Sbjct: 30 SELVNVAKKVRIDGFRKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGA 89 Query: 90 ALIFAAVVGVLMSTILYIAAPYYAEYS-----------KVPESTXXXXXXXXXXXXFPMI 138 + + Y E + E T Sbjct: 90 PTYVPGEYKLGEDFTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQA 149 Query: 139 AMMRGYFQGRNNMMAGGISQIIEQIARVSTAILLAFILLRQGYSNTWMAAG-ASFGSVLG 197 F+L Sbjct: 150 TWKEKDGAVEAEDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGE 209 Query: 198 SIGAFGVMLYYAVKLRRSEEQKALYDSSQARIPLLKIYKDIFKLSIPIVLSSVTVPVVNF 257 + + + + V + Sbjct: 210 EFTIDVTFPEEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAE 269 Query: 258 IDTSFIVPLLSGQIGREAATQALGI-----------FGSRAQSVAGIPPVLSIALSTSLI 306 + + A+ + A + L Sbjct: 270 VR----------KNMERELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAA 319 Query: 307 PVISAAYARREEAHLKRQITLALRISILTGTPIVLSLVVAAYSVNGLLFS---------- 356 + E + A R ++ + + + Sbjct: 320 QRFGGNEKQALELPRELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYE 379 Query: 357 SLDGSGIVAMLTLGTIFQITMMTTNSILLGMGKARISMYYVLAGLLVKFGSNFLLSRLFG 416 Sbjct: 380 439 Query: 417 IYGIIAATALCFIVITLLNLRMLKTIVPFE 446 Sbjct: 440 469 >lin0247 [R] COG2244 Membrane protein involved in the export of O-antigen and teichoic acid Length = 432 Score = 120 bits (302), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 0/423 (0%), Positives = 0/423 (0%), Gaps = 15/423 (3%) Query: 30 RVPLEHLFNDIGNASFTQANNVYLMLLPLATAGIPSTLSKMVSERYALNRPHEAQQVYRA 89 + I + + A+ M ++ + + Sbjct: 31 ELVNVAKKVRIDGFRKGKVPMNIVAQRYGASVRQDVLGDLMSRN--FIDAIIKEKINPAG 88 Query: 90 ALIFAAVVGVLMSTILYIAAPYYAEYSKVPESTXXXXXXXXXXXXFPMIAMMRGYFQGRN 149 A + L Y ++ + M + + Sbjct: 89 APTYVPGEYKLGEDFTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQ 148 Query: 150 NMMAGGISQIIEQIARVSTAIL---LAFILLRQGYSNTWMA-AGASFGSVLGSIGAFGVM 205 + + + G G Sbjct: 149 ATWKEKDGAVEAEDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIP---GFEDGIKGH 205 Query: 206 LYYAVKLRRSEE-QKALYDSSQARIPLLKIYKDIFKLSIPIVLSSVTVPVVNFIDTSFIV 264 ++ ++ + + + K L +T + Sbjct: 206 KAGEEFTIDVTFPEEYHAENLKGKA---AKFAINLKKVEERELPELTAEFIKRFGVEDGS 262 Query: 265 PLLSGQIGREAATQALGIFGSRAQSVAGIPPVLSIALSTSLIPVISAAYARREEAHLKRQ 324 R+ + L I ++ +I + +R Sbjct: 263 VEGLRAEVRKNMERELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRF 322 Query: 325 ITLALRISILTGTPIVLSLVVAAYSVNGLLFSSLDGSGIVAMLTLGTIFQITMMTTNSIL 384 + L L + + + Sbjct: 323 GGNEKQALELPRELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPK 382 Query: 385 LGMGKARISMYYVLAGLLVKF-GSNFLLSRLFGIYGIIAATALCFIVITLLNLRMLKTI- 442 + + + V Sbjct: 383 EVIEFYSKNKELMDNMRNVALE 442 Query: 443 VPF 445 Sbjct: 443 445 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.325 0.138 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,064,124 Number of extensions: 997232 Number of successful extensions: 4885 Number of sequences better than 1.0e-05: 30 Number of HSP's gapped: 4835 Number of HSP's successfully gapped: 30 Length of query: 542 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 431 Effective length of database: 25,576,357 Effective search space: 11023409867 Effective search space used: 11023409867 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 118 (50.1 bits)