BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0109.0008.p (267 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0497 [R] COG0561 Predicted hydrolases of the HAD superfamily 234 1e-61 BS_ycsE [R] COG0561 Predicted hydrolases of the HAD superfamily 215 4e-56 CAC0698 [R] COG0561 Predicted hydrolases of the HAD superfamily 142 5e-34 SP0923 [R] COG0561 Predicted hydrolases of the HAD superfamily 121 1e-27 FN0391 [R] COG0561 Predicted hydrolases of the HAD superfamily 105 5e-23 >BH0497 [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 247 Score = 234 bits (596), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 117/244 (47%), Positives = 167/244 (68%), Gaps = 1/244 (0%) Query: 6 MIAKYRLLALDMDGTLLNDEQQITPKTVEWIKKAMDAGVHVCLSTGRTSRGAMPYAEQLG 65 M Y+++ALDMDGTLL+ QI+ I++A + GVHV LSTGR+ + Y E L Sbjct: 1 MGGPYKIIALDMDGTLLSSNHQISEGNRRAIEEARNHGVHVILSTGRSFMTSKEYMESLA 60 Query: 66 LETPMIMVNGSEVWRTPSELYRRSLLDVTLVKTMHEIAEEYGTWFWACSVDEVYNRDNWD 125 L++ I VNGSE+W T EL R +L ++TM ++ E++ T FWA S ++V+ R + Sbjct: 61 LDSYHITVNGSEIWHTSGELIERQMLHGQHIETMWKLKEQHNTTFWAVSTEKVW-RGEFP 119 Query: 126 GDIEGREWLKFGYSTEDDDIRHKLLMRLQDLGGLEITNSSPTNLEINPLGINKASGIREV 185 +I EWLKFGY + +IR ++ LQ LE+TNSSPTNLEIN +G++KA + V Sbjct: 120 ENIHEHEWLKFGYDVPNQEIREQIEKELQAHSELEVTNSSPTNLEINAMGVSKAKALATV 179 Query: 186 CKLLGLEMSQVVAVGDSLNDLAAIQQSGFGVAMGNAQEVVKQEADAVVATNNDDGIAEVI 245 C+ LGL M+ V+A+GDSLNDLA I+++G GVAMGNAQE+VK+ AD V TN++DG+A+ I Sbjct: 180 CERLGLTMANVLAMGDSLNDLAMIEEAGCGVAMGNAQEIVKEAADWVTGTNDEDGVAQAI 239 Query: 246 QKYI 249 +K++ Sbjct: 240 RKFV 243 >BS_ycsE [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 249 Score = 215 bits (548), Expect = 4e-56, Method: Compositional matrix adjust. Identities = 111/239 (46%), Positives = 162/239 (67%), Gaps = 1/239 (0%) Query: 11 RLLALDMDGTLLNDEQQITPKTVEWIKKAMDAGVHVCLSTGRTSRGAMPYAEQLGLETPM 70 +L+A+DMDGTLLNDEQ I+ + + I++A D GV+V +STGRT AE L L + + Sbjct: 11 KLIAIDMDGTLLNDEQLISDENRKAIREAEDKGVYVVISTGRTLMTCRELAESLKLSSFL 70 Query: 71 IMVNGSEVWRTPSELYRRSLLDVTLVKTMHEIAEEYGTWFWACSVDEVYNRDNWDGDIEG 130 I NGSE+W + L R LL ++ M ++ ++ T FWA +V++V+ R + +I Sbjct: 71 ITANGSEIWDSNFNLVERKLLHTDHIQMMWDLRNKHNTNFWASTVNKVW-RGEFPENITD 129 Query: 131 REWLKFGYSTEDDDIRHKLLMRLQDLGGLEITNSSPTNLEINPLGINKASGIREVCKLLG 190 EWLKFG+ EDDDIR+++L L+ EITNSSPTN+E+N LGINKA+ + LG Sbjct: 130 HEWLKFGFDIEDDDIRNEVLEELRKTKSSEITNSSPTNIEVNALGINKAAAPCQGYGKLG 189 Query: 191 LEMSQVVAVGDSLNDLAAIQQSGFGVAMGNAQEVVKQEADAVVATNNDDGIAEVIQKYI 249 M V+A+GDSLND+A I+++G GVAMGNAQ++VK+ AD + TN +DG+A+ I+ ++ Sbjct: 190 FTMENVMAMGDSLNDIAMIKEAGLGVAMGNAQDIVKETADYITDTNIEDGVAKAIRHWV 248 >CAC0698 [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 276 Score = 142 bits (359), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 79/271 (29%), Positives = 145/271 (53%), Gaps = 30/271 (11%) Query: 9 KYRLLALDMDGTLLNDEQQITPKTVEWIKKAMDAGVHVCLSTGRTSRGAMPYAEQLGLET 68 KY+L+ +DMDGT+L+DE++I+ + +E +K+A DAGV + + TGR A+ Y++ LG++ Sbjct: 2 KYKLICVDMDGTVLDDEKKISNENIEAMKRAHDAGVKIAICTGRLFASALAYSDILGVKA 61 Query: 69 PMIMVNGSEVWRTPSE-LYRRSLLDVTLVKTMHEIAEEYGTWFWACSVDEV--------- 118 P+I NG + ++ + S L K ++ I E+Y + + + V Sbjct: 62 PLIASNGGYIREKDNDNVIYESYLKNEEAKDIYHIIEKYNVSMFFNTYNTVISNKEFEEN 121 Query: 119 YNRDNWDGDIEGREWLKFGYSTE------------------DDDIRHKLLMR--LQDLGG 158 Y + ++ ++ + +K Y D D+ L+R ++ L Sbjct: 122 YTYNKFNDNLPKEKRVKLVYPKNMDKFLEENVGEILKCICIDKDVEKLKLLRKEIESLNR 181 Query: 159 LEITNSSPTNLEINPLGINKASGIREVCKLLGLEMSQVVAVGDSLNDLAAIQQSGFGVAM 218 ++ +S N EI P G++K ++ + ++ +V+ +GD+ NDL+ I+ +G G+AM Sbjct: 182 FDVVSSGEFNFEIMPKGVSKGKAVKMLAAFYNIKQDEVICIGDNENDLSMIKCAGIGIAM 241 Query: 219 GNAQEVVKQEADAVVATNNDDGIAEVIQKYI 249 GNA++ +KQ AD V TNN+ G+A+ I K+I Sbjct: 242 GNAEDSIKQIADYVTDTNNNSGVAKAIDKFI 272 >SP0923 [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 269 Score = 121 bits (303), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 82/270 (30%), Positives = 137/270 (50%), Gaps = 35/270 (12%) Query: 11 RLLALDMDGTLLNDEQQITPKTVEWIKKAMDAGVHVCLSTGRTSRGAMPYAEQLGLETP- 69 +LLALDMDGTLLN+ ++I + I KA++ GV + L TGR G +PY ++L L+ Sbjct: 3 KLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGRPLFGVLPYYKKLELDLQN 62 Query: 70 --MIMVNGSEVWRT------------PSE------LYRRSLLDVTLVKTMH--------- 100 +I+ NG +T P++ L +S + +TL H Sbjct: 63 EYIIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKSDVQLTLFDESHYFVLGDKPN 122 Query: 101 EIAEEYGTWFWACSVDEVYNRDNWDGDIEGREWLKFGYSTEDDDIRHKLLMRL-QDLGGL 159 ++ E ++ + E+ + G + + G + DD + L Q G+ Sbjct: 123 QVIENDAKLVFS-DLTEISLEEATSGKFRMFQGMFLGTKEQTDDFEQRFAEELCQRFSGV 181 Query: 160 EITNSSPTNLEINPLGINKASGIREVCKLLGLEMSQVVAVGDSLNDLAAIQQSGFGVAMG 219 S P E PLG KA+ + + ++L ++ S+++A+GD+ ND+ +Q +G G+AMG Sbjct: 182 ---RSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAMGDANNDIEMLQFAGLGIAMG 238 Query: 220 NAQEVVKQEADAVVATNNDDGIAEVIQKYI 249 NA + +K ADAV ++N +DG+A I+KYI Sbjct: 239 NASDYIKSLADAVTSSNEEDGVARAIEKYI 268 >FN0391 [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 267 Score = 105 bits (263), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 83/258 (32%), Positives = 124/258 (48%), Gaps = 25/258 (9%) Query: 9 KYRLLALDMDGTLLNDEQQITPKTVEWIKKAMDAGVHVCLSTGRTSRGAMPYAEQLGLET 68 KY+L+ DMDGTLL +I+ T + IKK D GV ++TGR A Y + L L++ Sbjct: 2 KYKLVVCDMDGTLLTSNHKISDHTADVIKKIEDNGVKFMIATGRPYLDARYYRDSLKLKS 61 Query: 69 PMIMVNGSE----------VWRTPSELYRRSLLDVTLVKTMHEIAEEYGTWFWACSVDEV 118 +I NG+ + P E +R LL + K +H W ++ + Sbjct: 62 FLITSNGARAHDEDNNPIVIENIPKEFVKR-LLAYNVGKDIHRNIYLNDDWIIEYEIEGL 120 Query: 119 ----------YNRDNWDGDIEGREWLKFGYSTEDDDIRH-KLLMRLQDLGGLEITNSSPT 167 +N DN + E E K + +D+DI + + M + L IT SSP Sbjct: 121 VEFHKESGYRFNIDNLNK-YENEEAAKVFFLGKDEDIENLEKNMEKEFQNDLSITISSPF 179 Query: 168 NLEINPLGINKASGIREVCKLLGLEMSQVVAVGDSLNDLAAIQQSGFGVAMGNA-QEVVK 226 LE G+NKA +++V KLL +E +V+A GDS+ND + G MGNA Q ++ Sbjct: 180 CLEFMKKGVNKAETLKKVLKLLNIEPEEVIAFGDSMNDYEMLSLVGKPFIMGNANQRLID 239 Query: 227 QEADA-VVATNNDDGIAE 243 + VV NN+DGI + Sbjct: 240 ALPNVEVVGNNNEDGIGK 257 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.133 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,200,078 Number of extensions: 776958 Number of successful extensions: 3916 Number of sequences better than 1.0e-05: 124 Number of HSP's gapped: 3871 Number of HSP's successfully gapped: 160 Length of query: 267 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 162 Effective length of database: 26,268,361 Effective search space: 4255474482 Effective search space used: 4255474482 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)