BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0109.0005.p (381 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MJ1282.1 [NOU] COG1989 Type II secretory pathway, prepilin signa... 50 6e-06 MJ1282 [R] COG1800 Predicted transglutaminase-like proteases 50 6e-06 >MJ1282.1 [NOU] COG1989 Type II secretory pathway, prepilin signal peptidase PulO and related peptidases Length = 673 Score = 50.1 bits (118), Expect = 6e-06, Method: Compositional matrix adjust. Identities = 4/86 (4%), Positives = 7/86 (8%), Gaps = 4/86 (4%) Query: 239 ISPDIVGAAADIAGQAKGEEDKA--RRLYDWIGSRIAYDYGKAENYEQNRI-WHE-QTPQ 294 + L R + + Q Sbjct: 167 CYGLYDAHLKACNVLDFDDLILLPTLLLQANEEVRKRWQNKIRYLLVDEYQDTNTSQYEL 226 Query: 295 DTFDTRLGVCIDYARLYAVMARSQGL 320 S Sbjct: 227 VKLLVGSRARFTVVGDDDQSIYSWRG 252 >MJ1282 [R] COG1800 Predicted transglutaminase-like proteases Length = 252 Score = 50.1 bits (118), Expect = 6e-06, Method: Compositional matrix adjust. Identities = 8/86 (9%), Positives = 19/86 (22%), Gaps = 4/86 (4%) Query: 239 ISPDIVGAAADIAGQAKGEEDKA--RRLYDWIGSRIAYDYGKAENYEQNRI-WHE-QTPQ 294 G DI + + + + + EQ Q + Sbjct: 167 DILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLE 226 Query: 295 DTFDTRLGVCIDYARLYAVMARSQGL 320 + + + I A + + Sbjct: 227 EQYPLQENGQILLAFPRLFIVARRME 252 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.134 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,144,865 Number of extensions: 784020 Number of successful extensions: 1394 Number of sequences better than 1.0e-05: 2 Number of HSP's gapped: 1402 Number of HSP's successfully gapped: 2 Length of query: 381 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 273 Effective length of database: 25,922,359 Effective search space: 7076804007 Effective search space used: 7076804007 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)