BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0106.0010.p (185 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMb21003 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene te... 196 2e-50 AGc4197 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 196 2e-50 Cgl2071 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 147 1e-35 SA0327 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetr... 130 1e-30 Cgl1604 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 86 2e-17 >SMb21003 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 196 bits (498), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 12/178 (6%), Positives = 30/178 (16%), Gaps = 1/178 (0%) Query: 1 VELGISTFVETTPDAFTGETMSHAXXXXXXXXXXXXADQVGLDVYGVGEHHRPDFXXXXX 60 ++ G D + + Sbjct: 1 MQYHRIPHSSLEVST-LGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRP 59 Query: 61 XXXXXXXXXLTSRIRLTSAVMILSSADPVRVFQDFATLDGLSDGRAEIMVGRGSFTESFP 120 + + + + L A V D + R + +S Sbjct: 60 ETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLK 119 Query: 121 LFGCDLKDYETLFEEKLDLLMKLQQSERVSWEGRHRPAIHNLGIYPRPVQQPLPVWIA 178 D D + + + + + L + +I Sbjct: 120 RLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIG 177 >AGc4197 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 196 bits (497), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 7/178 (3%), Positives = 26/178 (14%) Query: 1 VELGISTFVETTPDAFTGETMSHAXXXXXXXXXXXXADQVGLDVYGVGEHHRPDFXXXXX 60 ++ TM+ V + + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 61 XXXXXXXXXLTSRIRLTSAVMILSSADPVRVFQDFATLDGLSDGRAEIMVGRGSFTESFP 120 + S ++ ++ ++ + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 121 LFGCDLKDYETLFEEKLDLLMKLQQSERVSWEGRHRPAIHNLGIYPRPVQQPLPVWIA 178 L L Y+ + ++ + + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGV 178 >Cgl2071 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 147 bits (370), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 17/182 (9%), Positives = 31/182 (17%), Gaps = 6/182 (3%) Query: 1 VELGISTFVETTPDAFTGETMSHAXXXXXXXXXXXXADQVGLDVYGVGEHHRPDFXXXXX 60 + S+ +T T A G+++ V E + Sbjct: 4 HRIPHSSLEVSTLG-LGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 61 XXXXXXXXXLTSRIRLTSAVMILSSADPVRVFQDFATLDGLSDGRAEIMVGRGSFTESFP 120 ++ ++I S D + R I + Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQ 122 Query: 121 LFGCDLKDYETLFEEKLDLLMKLQQSER----VSWEGRHRPAIHNLGIYPRPVQQPLPVW 176 DL VS + Sbjct: 123 TDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGK-IRYIGVSNE 181 Query: 177 IA 178 A Sbjct: 182 TA 183 >SA0327 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 130 bits (327), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 5/178 (2%), Positives = 16/178 (8%) Query: 1 VELGISTFVETTPDAFTGETMSHAXXXXXXXXXXXXADQVGLDVYGVGEHHRPDFXXXXX 60 + T + + V E Sbjct: 12 EVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGN 71 Query: 61 XXXXXXXXXLTSRIRLTSAVMILSSADPVRVFQDFATLDGLSDGRAEIMVGRGSFTESFP 120 S + + + + Sbjct: 72 WLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQV 131 Query: 121 LFGCDLKDYETLFEEKLDLLMKLQQSERVSWEGRHRPAIHNLGIYPRPVQQPLPVWIA 178 + + + + V Sbjct: 132 HWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRY 189 >Cgl1604 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 86.3 bits (212), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 7/177 (3%), Positives = 27/177 (15%), Gaps = 1/177 (0%) Query: 1 VELGISTFVETTPDAFTGETMSHAXXXXXXXXXXXXADQVGLDVYGVGEHHRPDFXXXXX 60 ++ TM+ V + + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 61 XXXXXXXXXLTSRIRLTSAVMILSSADPVRVFQDFATLDGLSDGRAEIMVGRGSFTESFP 120 + S ++ ++ ++ + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 121 LFGCDLKDYETLFEEKLDLLMKLQQSERVSWEGRHRPAIHNLGIYPRPVQQPLP-VW 176 L L Y+ + ++ + + + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIG 177 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.138 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,932,093 Number of extensions: 400138 Number of successful extensions: 453 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 451 Number of HSP's successfully gapped: 10 Length of query: 185 Length of database: 38,378,431 Length adjustment: 100 Effective length of query: 85 Effective length of database: 26,845,031 Effective search space: 2281827635 Effective search space used: 2281827635 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 112 (47.8 bits)