BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0105.0011.p (508 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin1658 [GEPR] COG0477 Permeases of the major facilitator superf... 415 e-116 BS_lmrB [GEPR] COG0477 Permeases of the major facilitator superf... 363 e-100 lin0519 [GEPR] COG0477 Permeases of the major facilitator superf... 331 2e-90 L132251 [GEPR] COG0477 Permeases of the major facilitator superf... 243 6e-64 BH0554 [GEPR] COG0477 Permeases of the major facilitator superfa... 183 5e-46 >lin1658 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 489 Score = 415 bits (1067), Expect = e-116, Method: Compositional matrix adjust. Identities = 209/478 (43%), Positives = 306/478 (64%), Gaps = 1/478 (0%) Query: 16 EAISSSVKKGPILFIMILGAFLATLNQTVMSVATPELMHDFNITAATAQWLTTGYMLVNG 75 +A S+SVK+ IL +M++GAF+ LNQT+M+VA P +M DF ITA+ QWL+TG+MLVNG Sbjct: 2 KAASTSVKRNGILIVMLMGAFVTILNQTLMNVALPSIMKDFGITASQGQWLSTGFMLVNG 61 Query: 76 VLIPITAFLMQRFTTRELFQSSMFIFLAGTVVSALATSFPILLTGRMIQAAGAGIIMPLL 135 V+IP+TAFL++RFTTR+L+ +M F GT + AT + +L+ GRM+QA GAGI+MPLL Sbjct: 62 VMIPMTAFLIERFTTRQLYLFAMITFAIGTAIGGFATDYTMLIAGRMVQAIGAGIVMPLL 121 Query: 136 TNVILTLFPREKRGAAMGMVGLAIIFAPAIGPTLAGYILEHYSWETMFYGMLPLTAIVII 195 T V+L LFP E+RG AMG++GLA+ FAPAIGPTL+G+I++ Y W +F+ ++P + I+ Sbjct: 122 TVVVLNLFPMERRGRAMGLIGLAMNFAPAIGPTLSGWIVQQYDWRNLFFIIIPFAILDIV 181 Query: 196 CGFIYLKNVAERSYPKIDLASVILSTVGFGTLLYGFSRAASAGWSSAEVIATLAAGVLSL 255 LKNV +R++PK+D+ VI+STVGFG+LL GFS A W + +V + G++ L Sbjct: 182 VAIFLLKNVGKRTFPKLDILGVIMSTVGFGSLLLGFSNAGDHDWLTWKVAGFIILGLVVL 241 Query: 256 GLFTWRQLASSSPLLDLRVFKYNMFSLTTIINIAVTIIMYADMMLLPLYLQNARGYTAME 315 G+F Q ++ +PLL+ +VFKY F+LTT I+ V + ++ M+LLP++LQ RG++ +E Sbjct: 242 GIFIRYQSSNKAPLLNFKVFKYPTFALTTAISFFVVMGLFGGMLLLPIFLQTVRGFSPLE 301 Query: 316 SXXXXXXXXXXXXXXXXVTGKLFDRFGAKWXXXXXXXXXXXXXXSFVNLTDSTNYTYLVL 375 S VTG +FDRFGAK+ F NL +ST T++++ Sbjct: 302 SGLVLLPGALVTAILSPVTGVMFDRFGAKYLSLVGLIIMAGSTFMFTNLDESTTLTFIII 361 Query: 376 MSTGRRIGMALLLMPIQTLGLNQLPSKLNAHGTAISNTIRQVAGAVGTSLLVTVMTSRTT 435 + T R GMA+++MP+QT LN LP KL +HG+A+ NT+RQVAG++GT+ L+TVM S++ Sbjct: 362 VQTIRSAGMAMVMMPLQTAALNSLPLKLASHGSAMFNTMRQVAGSIGTAALITVM-SKSA 420 Query: 436 THLXXXXXXXXXXXXSKEHMIMEASIQGINDAYXXXXXXXXXXXXXAFFIKRVGQASD 493 +K + I GI A+ A FI++ A D Sbjct: 421 ASFADKLGPADVIGKTKTEVANHVLIHGIETAFLVAGILSVVACILALFIQKNRSAMD 478 >BS_lmrB [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 479 Score = 363 bits (932), Expect = e-100, Method: Compositional matrix adjust. Identities = 191/478 (39%), Positives = 286/478 (59%), Gaps = 8/478 (1%) Query: 13 NKTEAISSSVKKGPILFIMILGAFLATLNQTVMSVATPELMHDFNITAATAQWLTTGYML 72 T S K PI+ ++L F+ ++T +++A +LM + NITAAT QWLTTGY+L Sbjct: 4 ETTAKASQQYKVMPIMISLLLAGFIGMFSETALNIALTDLMKELNITAATVQWLTTGYLL 63 Query: 73 VNGVLIPITAFLMQRFTTRELFQSSMFIFLAGTVVSALATSFPILLTGRMIQAAGAGIIM 132 V G+L+P++ L+Q FTTR+LF S+ + GT ++ALA SF LL R++QA G G+++ Sbjct: 64 VLGILVPVSGLLLQWFTTRQLFTVSLIFSILGTFIAALAPSFSFLLAARIVQALGTGLLL 123 Query: 133 PLLTNVILTLFPREKRGAAMGMVGLAIIFAPAIGPTLAGYILEHYSWETMFYGMLPLTAI 192 PL+ N IL +FP KRGAAMG +GL I+FAPAIGPT +G +LEH +W +F+ LP + Sbjct: 124 PLMFNTILVIFPPHKRGAAMGTIGLVIMFAPAIGPTFSGLVLEHLNWHWIFWISLPFLVL 183 Query: 193 VIICGFIYLKNVAERSYPKIDLASVILSTVGFGTLLYGFSRA--ASAGWSSAEVIATLAA 250 ++ G Y++NV+E + PKID+ S+ILST+GFG +++GFS A S GWSS VI +L Sbjct: 184 ALVFGIAYMQNVSETTKPKIDVLSIILSTIGFGGIVFGFSNAGEGSGGWSSPTVIVSLIV 243 Query: 251 GVLSLGLFTWRQLASSSPLLDLRVFKYNMFSLTTIINIAVTIIMYADMMLLPLYLQNARG 310 GV+ L LF+ RQL P+++LR FKY MF L I+ +++ + M+LLP+YLQ Sbjct: 244 GVVGLILFSIRQLTMKQPMMNLRAFKYPMFILGVIMVFICMMVILSSMLLLPMYLQGGLV 303 Query: 311 YTAMESXXXXXXXXXXXXXXXXVTGKLFDRFGAKWXXXXXXXXXXXXXXSFVNLTDSTNY 370 TA S VTG+LFD++G KW F N+T ++ Sbjct: 304 LTAFASGLVLLPGGILNGFMSPVTGRLFDKYGPKWLVIPGFVIVTVVLWFFSNVTTTSTA 363 Query: 371 TYLVLMSTGRRIGMALLLMPIQTLGLNQLPSKLNAHGTAISNTIRQVAGAVGTSLLVTVM 430 ++++ T IG+++++MP QT GLNQLP + GTAI NT++Q+AGA+GT++ V++M Sbjct: 364 VLIIILHTCLMIGISMIMMPAQTNGLNQLPREFYPDGTAIMNTLQQMAGAIGTAVAVSIM 423 Query: 431 TSRTTTHLXXXXXXXXXXXXSKEHMIMEASIQGINDAYXXXXXXXXXXXXXAFFIKRV 488 + ++ + +I +A G+ A+ AFF+KRV Sbjct: 424 AAGQHDYM------STVKNPADPAVIPQALTAGVQHAFVFAMIVAIIGLIGAFFMKRV 475 >lin0519 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 471 Score = 331 bits (848), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 172/468 (36%), Positives = 283/468 (60%), Gaps = 7/468 (1%) Query: 20 SSVKKGPILFIMILGAFLATLNQTVMSVATPELMHDFNITAATAQWLTTGYMLVNGVLIP 79 ++ PI+ ++ F+ ++T +++A +L+ F+I++AT QWLTTGY+L G+L+P Sbjct: 9 QKIRPIPIIASFLMAGFIGLFSETALNMALSDLIQVFDISSATVQWLTTGYLLTLGILVP 68 Query: 80 ITAFLMQRFTTRELFQSSMFIFLAGTVVSALATSFPILLTGRMIQAAGAGIIMPLLTNVI 139 I+ L+Q FTTR LF +++ +AGT+++AL+ +F +L+ GR++QA G +++PL+ N I Sbjct: 69 ISGLLLQWFTTRGLFFTAVSFSIAGTLIAALSPTFAMLMIGRVVQAVGTALLLPLMFNTI 128 Query: 140 LTLFPREKRGAAMGMVGLAIIFAPAIGPTLAGYILEHYSWETMFYGMLPLTAIVIICGFI 199 L +FP KRG+AMGM+GL I+FAPA+GPT++G ILE+ +W +F+ LP I ++ G Sbjct: 129 LLIFPEHKRGSAMGMIGLVIMFAPAVGPTISGLILENLTWNWIFWISLPFLIIALLFGMK 188 Query: 200 YLKNVAERSYPKIDLASVILSTVGFGTLLYGFSRAASAGWSSAEVIATLAAGVLSLGLFT 259 +++NV+ + PKID+ S+ILST+GFG +++ FS A GW SA V+ ++ G ++LGLF Sbjct: 189 FMQNVSVVTKPKIDILSIILSTLGFGGVVFAFSSAGENGWGSATVLVSIIVGGIALGLFV 248 Query: 260 WRQLASSSPLLDLRVFKYNMFSLTTIINIAVTIIMYADMMLLPLYLQNARGYTAMESXXX 319 WRQL PL+DL+VFKY MF+L I+ +++ + M+LLPLYLQN+ A + Sbjct: 249 WRQLTMEKPLMDLKVFKYPMFTLGLILVFISFMMILSTMILLPLYLQNSLALAAFSAGLV 308 Query: 320 XXXXXXXXXXXXXVTGKLFDRFGAKWXXXXXXXXXXXXXXSFVNLTDSTNYTYLVLMSTG 379 TG+LFD +G + + T+ ++++ + Sbjct: 309 LLPGGVLNGLMSPFTGRLFDAYGPRALVIPGFIVAVIALFFLTRIEVGTSALTIIVLHSV 368 Query: 380 RRIGMALLLMPIQTLGLNQLPSKLNAHGTAISNTIRQVAGAVGTSLLVTVMTSRTTTHLX 439 IG+++++MP QT GLNQLP KL GTAI NT++QV+GA+GT++ +T+M++ ++ Sbjct: 369 LMIGISMVMMPAQTNGLNQLPPKLYPDGTAIMNTLQQVSGAIGTAVAITIMSAGQKAYM- 427 Query: 440 XXXXXXXXXXXSKEHMIMEASIQGINDAYXXXXXXXXXXXXXAFFIKR 487 E MI + GI +A+ + FI++ Sbjct: 428 -----ETAQGVGPEQMIASLT-AGIQNAFVFGLIMACIGLLCSLFIRK 469 >L132251 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 514 Score = 243 bits (620), Expect = 6e-64, Method: Compositional matrix adjust. Identities = 153/473 (32%), Positives = 244/473 (51%), Gaps = 14/473 (2%) Query: 27 ILFIMILGAFLATLNQTVMSVATPELMHDFNITAATAQWLTTGYMLVNGVLIPITAFLMQ 86 +L ++++G F A L QT + A P LM+DFNI+ +TAQ TT ++L NG+++PI+A+L Sbjct: 23 MLILILVGTFGAMLMQTSLGTAIPTLMNDFNISMSTAQQATTWFLLANGIMVPISAYLAT 82 Query: 87 RFTTRELFQSSMFIFLAGTVV--SALATSFPILLTGRMIQAAGAGIIMPLLTNVILTLFP 144 +F TR L+ ++ G V SA +S+ + L GR++QA GI MPL+ ++ +FP Sbjct: 83 KFPTRWLYVTAYATLFIGMFVAYSAPTSSWWVFLAGRILQAIAVGITMPLMQVALVNMFP 142 Query: 145 REKRGAAMGMVGLAIIFAPAIGPTLAGYILEHY----------SWETMFYGMLPLTAIVI 194 ++ GA MG+ G+ ++ APA+GPT AG+++ H SW T+F + + +V Sbjct: 143 AKQMGAIMGVAGVVLVLAPALGPTFAGWLITHKFNLIFWTFAASWRTIFLIPMIVVGVVF 202 Query: 195 ICGFIYLKNVAERSYPKIDLASVILSTVGFGTLLYGFSRAASAGWSSAE-VIATLAAGVL 253 + +++V K+D SV+LS VGFG L+GF+ A+ GW VIA + G++ Sbjct: 203 LISIFAMRDVIPNRPMKLDFLSVLLSVVGFGAFLWGFTNVATDGWGKFNTVIAPIVGGLI 262 Query: 254 SLGLFTWRQLASSSPLLDLRVFKYNMFSLTTIINIAVTIIMYADMMLLPLYLQNARGYTA 313 + LF RQL P LD+RVFK F+L T+ T+ M M+LPLYLQ+ G +A Sbjct: 263 IIFLFGLRQLKMDDPFLDIRVFKIRQFTLVTLALALSTMAMMGVEMMLPLYLQDVHGLSA 322 Query: 314 MESXXXXXXXXXXXXXXXXVTGKLFDRFGAKWXXXXXXXXXXXXXXSFVNLTDSTNYTYL 373 S + G ++DR G+K F+ T +T + Sbjct: 323 FNSGLVLLPGSLMMAVVAPLAGGVYDRIGSKRLAVIGFLVLALGTIPFMFFTATTPDHFD 382 Query: 374 VLMSTGRRIGMALLLMPIQTLGLNQLPSKLNAHGTAISNTIRQVAGAVGTSLLVTVMTSR 433 L+ R +G+ +++MP+ T +N +P A TA +NT RQVA +V +LL +V + Sbjct: 383 TLLYGLRMVGVGMVMMPLTTSAMNAVPVNEVAQATASNNTARQVASSVVVALLSSVAQNV 442 Query: 434 TTTHLXXXXXXXXXXXXSKEHMIMEASIQGINDAYXXXXXXXXXXXXXAFFIK 486 T ++ I EA +QG + ++ A F+K Sbjct: 443 ITNNMPASSLKSTNPLQFANKAI-EAMLQGYHASFAIGLVFAIIGIGVALFLK 494 >BH0554 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 474 Score = 183 bits (465), Expect = 5e-46, Method: Compositional matrix adjust. Identities = 127/475 (26%), Positives = 224/475 (47%), Gaps = 15/475 (3%) Query: 23 KKGPILFIMILGAFLATLNQTVMSVATPELMHDFNITAATAQWLTTGYMLVNGVLIPITA 82 KK ++ ++ G+F LN ++++ A P+LM+ F A W+ T +M+ G+ +P+T Sbjct: 6 KKWMVVCAVLFGSFTMILNNSMLNPAIPQLMNVFEADAVATGWVITIFMVAMGMTMPLTG 65 Query: 83 FLMQRFTTRELFQSSMFIFLAGTVVSALATSFPILLTGRMIQAAGAGIIMPLLTNVILTL 142 +L + +E + + IF+ G+++ AL+ + P L+ R +Q G G++MPL +I Sbjct: 66 YLGDKIGKKEAYILGLGIFVLGSLLGALSWNLPSLIVFRGLQGIGGGVMMPLSMTLIFDA 125 Query: 143 FPREKRGAAMGMVGLAIIFAPAIGPTLAGYILEHYSWETMFYGMLPLTAIVIICGFIYLK 202 FPR +RG A G+ G+A + AP IGPTL G+I+E +W+ +F +P + II IYL Sbjct: 126 FPRNERGLATGVWGVASMMAPTIGPTLGGFIVETSNWKYLFLVNIPFGLLGIIAAVIYLP 185 Query: 203 NVAERSYPKIDLASVILSTVGFGTLLYGFSRAASAGWSSAEVIATLAAGVLSLGLFTWRQ 262 +A K+D + T G G++L F R + E I + + +L L+ + Q Sbjct: 186 KIARSGQIKLDRWGFLFVTAGVGSILLAFGRMSDLT-HLTEWINGVLLLLGALCLYVFVQ 244 Query: 263 LA--SSSPLLDLRVFKYNMFSLTTIINIAVTIIMYADMMLLPLYLQNARGYTAMESXXXX 320 + + PLLDL +F+ +SL+ + +I ++A + L+PL LQ Y + + Sbjct: 245 VEKRAEQPLLDLSLFRIPAYSLSIWVAGISSIGLFAGIFLVPLLLQQVYDYGPIMTGLVF 304 Query: 321 XXXXXXXXXXXXVTGKLFDRFGAKWXXXXXXXXXXXXXXSFVNLTDSTNYTYLVLMSTGR 380 + G++ D+ G + L T Y+ R Sbjct: 305 LPSALFTGLTMSIGGRMLDKRGPSGIMTAGMMIAAVGTFALGYLHLETGLWYIFAWMAIR 364 Query: 381 RIGMALLLMPIQTLGLNQLPSKLNAHGTAISNTIRQVAGAVGTSLLVTVMTSRTTTHLXX 440 +GM L MP T G+N +P L + G+A++N +RQ++ A G + R Sbjct: 365 GVGMGLTTMPATTTGMNAIPEGLISRGSAMNNVLRQMSSAFGIVFISVFFEVRRGQ---- 420 Query: 441 XXXXXXXXXXSKEHMIMEASIQGINDAYXXXXXXXXXXXXXAFFIKRVGQASDQE 495 S E EA++Q IN+ + A+++++ + +QE Sbjct: 421 ----LALVSTSFE----EATLQAINEGFFIVGFLTALSIPAAYWLEKKAKQGEQE 467 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,638,350 Number of extensions: 991081 Number of successful extensions: 11179 Number of sequences better than 1.0e-05: 101 Number of HSP's gapped: 11123 Number of HSP's successfully gapped: 101 Length of query: 508 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 397 Effective length of database: 25,576,357 Effective search space: 10153813729 Effective search space used: 10153813729 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 118 (50.1 bits)