BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0105.0005.p (423 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Cgl2408 [G] COG1653 ABC-type sugar transport system, periplasmic... 95 2e-19 SP1897 [G] COG1653 ABC-type sugar transport system, periplasmic ... 94 4e-19 BS_yurO [G] COG1653 ABC-type sugar transport system, periplasmic... 85 3e-16 PH1214 [G] COG1653 ABC-type sugar transport system, periplasmic ... 84 6e-16 BS_msmE [G] COG1653 ABC-type sugar transport system, periplasmic... 70 1e-11 >Cgl2408 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 443 Score = 95.1 bits (235), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 105/421 (24%), Positives = 169/421 (40%), Gaps = 60/421 (14%) Query: 37 VHIYQFKVEISEALAKLKTEFEKEHPDIRLEIQSVGGGSDYGASLRAKFSSGDEPDIFTI 96 V+ FK E A ++ + E +++++ + GS Y +L+A+ + P +F + Sbjct: 34 VYFLNFKPEQDVAYQEIAKAY-TEETGVKVKVVTAASGS-YEQTLKAEIGKDEAPTLFQV 91 Query: 97 GGNNERDMWLEYLEDLSGEAWAK----DVKPLAAEQMTVNGKLYGMPMNLEGYGFIYNKD 152 G W +Y+ D+S AK D+ PL E +G++ G+P +EG+G IYN + Sbjct: 92 NGPAGFITWQDYMADMSDTEVAKQLTDDIPPLTTE----DGEVRGVPFAVEGFGIIYNDE 147 Query: 153 LFKQAGITQKPXXXXXXXXXXXXXXXXGITPFANGYQEWFVLGNHNVNVAFAQQPDPESF 212 +F + T E + G AFA Sbjct: 148 IFDKYIATSGAKIKSTDEITSYQKLKEVAEDMQAKKDELGIEG------AFAS------- 194 Query: 213 IAGLTDG-----SKHFAGDPVFSKWLD-------------------LLDLTVEYGN-KNP 247 LT G H A P++ ++ D L DL +E + Sbjct: 195 -TSLTSGEDWRWQTHLANAPIWQEYQDKGVEDTNEIEFSYNKEYKNLFDLYLENSTVEKS 253 Query: 248 LSTDYNTQVTMV--ASGEAAMMQQGNWTQGQIDGIDPDL----NLGILPMPIDDTPEDND 301 L+ +M A G+AAM+Q GNW QI ++ + LPM + E+ Sbjct: 254 LAPSKTVSDSMAEFAQGKAAMVQNGNWAWSQISETSGNVVKEDKIKFLPMYMGLPDEEKH 313 Query: 302 KLYVGVPSNWVVN-KFSPVKEEA-KVFLDWLVTSETGKKYITKEFQFIPALDSIQGTKED 359 + VG + VN + S V ++A K F+DWL TSE GK+++ K+ FI +S Sbjct: 314 GINVGTENYLGVNSEASEVDQQATKDFVDWLFTSEAGKEHVVKDLGFIAPFESYTAENTP 373 Query: 360 LGALGSEVLEYTDKDQALT--WNWSRLPN-GMPQELSSNIQGYLGGKLTKEGMLKEFQVN 416 L +V E T WN+ P+ + ++ Y GKL E ++K F N Sbjct: 374 NDPLSEQVAEAIANKDLTTYPWNFQYFPSQQFKDDFGQDLSQYASGKLKWEDVVKHFNDN 433 Query: 417 W 417 W Sbjct: 434 W 434 >SP1897 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 419 Score = 94.0 bits (232), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 91/364 (25%), Positives = 159/364 (43%), Gaps = 32/364 (8%) Query: 31 AGGIKTVHIYQFKVEISEALAKLKTEFEKEHPDIRLEIQSVGGGSDYGASLRAKFSSGDE 90 A G T+ + K E+++ L ++ +FEKE+P I++++ +V + G L+ + +GD Sbjct: 30 ADGTVTIEYFNQKKEMTKTLEEITRDFEKENPKIKVKVVNV---PNAGEVLKTRVLAGDV 86 Query: 91 PDIFTIGGNN-ERDMWLE--YLEDLSGEAWAKDVKPLAAEQMTVNGKLYGMPMNLEGYGF 147 PD+ I + E W + EDLS + + K VK AE+ VN K+Y +P YG Sbjct: 87 PDVVNIYPQSIELQEWAKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGI 146 Query: 148 IYNKDLFKQAGITQKPXXXXXXXXXXXXXXXXGITPFANGYQEWFVLGNHNVNVAFAQQP 207 YNKD F++ G+ + P G TPF + + L +N +AFA Sbjct: 147 YYNKDKFEELGL-KVPETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYN-QLAFA--- 201 Query: 208 DPESFIAGLTDGSKHFA---------GDPVFSKWLDLLDLTVEYGN--KNPLSTDYNTQV 256 + G + +++ DP+ + ++D+ G+ KN Y + Sbjct: 202 ---TATGGGKEANQYLRYSQPNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVI 258 Query: 257 TMVASGEAAMMQQGNWTQGQIDGIDPDLNLGILPMPIDDTPEDNDKLYVGVPS-NWVVNK 315 A G+ M G+W I+ P+ +G +P E L VG W ++ Sbjct: 259 GAFARGDVLMTPNGSWAITAINEQKPNFKIGTFMIP---GKEKGQSLTVGAGDLAWSISA 315 Query: 316 FSPVKEEAKVFLDWLVTSETGKKYITKEFQFIPALDSIQGTKEDLGALGSEVLEYTDKDQ 375 + +EA F++++ E +KY + A++ ++ ED G + EY D+ Sbjct: 316 TTKHPKEANAFVEYMTRPEVMQKYYDVDGS-PTAIEGVKQAGEDSPLAG--MTEYAFTDR 372 Query: 376 ALTW 379 L W Sbjct: 373 HLVW 376 >BS_yurO [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 422 Score = 84.7 bits (208), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 85/372 (22%), Positives = 158/372 (42%), Gaps = 28/372 (7%) Query: 56 EFEKEHPDIRLEIQSVGGGSDYGASLRAKFSSGDEPDIFT----------IGGNNERDMW 105 EFEK+HPDI ++I++V S Y ++ + PDI+ I GN D+ Sbjct: 55 EFEKDHPDIDIQIEAVLNDS-YKDKIKVMLGTTSPPDIYFSWSDEFAFKFIRGNKALDLS 113 Query: 106 LEYLEDLSGEAWAKDVKPLAAEQMTVNGKLYGMPMNLEGYGFIYNKDLFKQAGITQKPXX 165 Y D W+ + T K YG+P ++ F YNKD+F++ + P Sbjct: 114 SYYKNDTD---WSSQLVQSQITPFTYENKQYGVPWQMDAKSFFYNKDIFQKLNL-DPPKT 169 Query: 166 XXXXXXXXXXXXXXGITPFANGYQEWFVLGNHNVNVAFAQQPDPESFIAGLTDGSKHFAG 225 G TP + G + + + +H + + D ++ + F Sbjct: 170 WDELIDVSKKLKEHGYTPISFGTKATWTI-SHYIGTLNQRMVDEKTREKDYNAKTGEFT- 227 Query: 226 DPVFSKWLDLLDLTVEYGNKNPLSTDYNTQVTMVASGEAAMMQQGNWTQGQIDGIDPDLN 285 D + K L+ L + Y NK+ S D+ SG++AM+ +I ++P +N Sbjct: 228 DEGYVKALEKLQELMPYFNKHVNSVDHEYVRQQFKSGKSAMIYAET---AEIKLVEP-VN 283 Query: 286 LGILPMPIDDTPEDNDKLYVGVPSNWVVNKFSPVKEEAKVFLDWLVTSETGKKYITKEFQ 345 LG+ P P + + + G P ++++ + +EA FL +L + G+K + Sbjct: 284 LGMFPFPEISGQKGSSEALTGAPEGFMISSRTKHPKEAMEFLQFLTSKRMGEKLVKD--- 340 Query: 346 FIPALDSIQG--TKEDLGALGSEVLEY-TDKDQALTWNWSRLPNGMPQELSSNIQGYLGG 402 + ++QG T+E+ A+ E +++ D + W + + + +Q LGG Sbjct: 341 -VGKYSAVQGTATEENATAIQREAVQHIVDAKSMVPWFDMDVDVEVADAYLTGVQQMLGG 399 Query: 403 KLTKEGMLKEFQ 414 +T + ++K Q Sbjct: 400 DMTPQQVMKAVQ 411 >PH1214 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 441 Score = 83.6 bits (205), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 74/262 (28%), Positives = 116/262 (44%), Gaps = 26/262 (9%) Query: 48 EALAKLKTEFEKEHPDIRLEIQSV--GGGSDYGASLRAKFSSGDEPDIFTIGGNNERDMW 105 EA L +FEK++P+I+++ V GGG + L++ +G PD F + E + Sbjct: 57 EAFDALAKKFEKKYPNIKIKANPVSGGGGVNLKMVLQSLMLAGKPPDTFQVHAGYEMARY 116 Query: 106 LEY-----LEDLSGEAWAKDVK---PLAAEQMTV-NGKLYGMPMNLEGYGFI-YNKDLFK 155 ++ ++D+ W +D+K P +QM + N Y +P+N+ I YNK +FK Sbjct: 117 IKANQLTPIDDI----WTEDMKKNYPKVIQQMVIFNNHYYAVPINVHRANVIWYNKQIFK 172 Query: 156 QAGITQKPXXXXX-XXXXXXXXXXXGITPFANG-YQEWFVLGNHNVN-VAFAQQPDPESF 212 + GI G+TPFA G +W V VA E F Sbjct: 173 KYGIDPSSIKTIDDLFAVAEKLKENGVTPFALGDRNKWPATQVFEVMLVAVGGVDYYEKF 232 Query: 213 IAGLTDGSKHFAGDPVFSKWLDLLDLTVEYGNKNPLSTDYNTQVTMVASGEAAMMQQGNW 272 I G A DP K L+ VEY N + + ++ +V GEAAM G+W Sbjct: 233 INGEIS-----ANDPTLKKVLEYFVKYVEYSNPDHAAKTWDEACALVYKGEAAMTLMGDW 287 Query: 273 TQG--QIDGIDPDLNLGILPMP 292 G + G P+++ G +P+P Sbjct: 288 ANGYFKAKGWKPNVDYGAIPIP 309 >BS_msmE [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 426 Score = 69.7 bits (169), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 96/385 (24%), Positives = 163/385 (42%), Gaps = 42/385 (10%) Query: 35 KTVHIYQFKVEISE--ALAKLKTEFEKEHPDIRLEIQSVGGGSDYGASLRAKFSSGDEPD 92 KT+ IY SE KL FEKEH DI + + G+DY +R + ++ D PD Sbjct: 31 KTLTIYSTMSTDSERDTFRKLAAAFEKEHSDIHVSLHF--PGNDYENMMRVRMAANDLPD 88 Query: 93 IFTIGGNNERDMWLEYLEDLSGEAWAKDVKP-LAAEQMTVNGKLYGMPMNLEGYGFIYNK 151 +F G + + EY DL W +D+ P L + +GK+Y P+N G YN+ Sbjct: 89 LFDTHGWG-KIRYGEYTADLRDMKWTQDLDPNLNSILKNKSGKVYAYPINQAKDGLAYNR 147 Query: 152 DLFKQAGIT--QKPXXXXXXXXXXXXXXXXGITPFANGYQEWFVLGNHNVNVAFAQQPDP 209 ++ + GI + I PF WF + + +FAQ D Sbjct: 148 NILDRYGIAPPETMDDFIKALRTIKEKSKGSIVPF------WFAGYDKS---SFAQYYDQ 198 Query: 210 ESFIAGLTDGSKH-----FAGDPVFSKWLDLLDLTVEYGNKNPLSTDYNTQ-----VTMV 259 + +TD + + G +SK+ L ++ + + ++ D T + ++ Sbjct: 199 FATPLLITDPAHNEKKQLINGTFQWSKFTYLSEILKQMQKEKLINIDAVTAKKSQLIELM 258 Query: 260 ASGEAAMMQQGNWTQGQIDGIDPDLNLGILPMPIDDTPEDNDKLYVGVPSNWVVN-KFSP 318 A + A QG + I+P++ +GI+P P P D D +++G + K SP Sbjct: 259 AQNKIAFTMQGGTLGQDVAQINPNVKVGIIPTPAIH-PGD-DPIWIGGERYTLAAWKDSP 316 Query: 319 VKEEAKVFLDWLVTSETGKKYITKEFQFIPALDSIQGTKEDLGALGSEVLEYTDKDQALT 378 +EAK F+ ++ K+ E +P+ + K D+ ++ Y D Sbjct: 317 QLKEAKDFIAFMARPANAKQ--MAEATSLPS--GLTNVKADI-FYANDYEYYQDVKVEPY 371 Query: 379 WNWSRLPNGM-------PQELSSNI 396 ++ LPNGM QEL+++I Sbjct: 372 FDRLYLPNGMWDVLGTVGQELAADI 396 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.135 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,883,769 Number of extensions: 1374920 Number of successful extensions: 2181 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 2182 Number of HSP's successfully gapped: 13 Length of query: 423 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 314 Effective length of database: 25,807,025 Effective search space: 8103405850 Effective search space used: 8103405850 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 117 (49.7 bits)