BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0101.0018.p (259 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3849 [R] COG0656 Aldo/keto reductases, related to diketogulona... 341 6e-94 lin0819 [R] COG0656 Aldo/keto reductases, related to diketogulon... 326 2e-89 BH2158 [R] COG0656 Aldo/keto reductases, related to diketogulona... 322 4e-88 BS_ytbE [R] COG0656 Aldo/keto reductases, related to diketogulon... 316 3e-86 BS_yvgN [R] COG0656 Aldo/keto reductases, related to diketogulon... 304 1e-82 >BH3849 [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 341 bits (875), Expect = 6e-94, Method: Compositional matrix adjust. Identities = 18/260 (6%), Positives = 46/260 (17%), Gaps = 1/260 (0%) Query: 1 MPWLGLGVWQAKEGDEVIHAVKTALETGYRSIDTAAGYKNEEGVGQAIRESGVPREELFI 60 Q E D + Q + E+ V Sbjct: 17 GLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKH 76 Query: 61 TTKLWNGDQGYESALKAFEVSRKKLGLDVLDLYLIHWPVAGKYRESWKALVHLQKEGLVK 120 ++ S + + + R L Q + Sbjct: 77 GSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQR 136 Query: 121 SIGVSNFQPHHLQDIIDDTGVVPAVDQVEFHPLLTQRELLKYAKAHDIQVEAWSPLMQGN 180 + D ++ +D + + + + + V + L + Sbjct: 137 PTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKH 196 Query: 181 L-DLPVLKELAGKYGKTPAQIVLRWDLQQGVITIPKSVHAERIVENAGFFDFTLSDEDVK 239 V + ++ L Q + + + + Sbjct: 197 DLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARN 256 Query: 240 VLEDLNQNHRFGPDPDNFNF 259 L + Sbjct: 257 TLFSRFTRYSGEQTQKAVAA 276 >lin0819 [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 326 bits (835), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 15/260 (5%), Positives = 45/260 (17%), Gaps = 1/260 (0%) Query: 1 MPWLGLGVWQAKEGDEVIHAVKTALETGYRSIDTAAGYKNEEGVGQAIRESGVPREELFI 60 LG + + + HA ++ A + + Sbjct: 15 TLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLA 74 Query: 61 TTKLWNGDQGYESALKAFEVSRKKLGLDVLDLYLIHWPVAGKYRESWKALVHLQKEGLVK 120 + K + D + + + Sbjct: 75 KHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWP 134 Query: 121 SIGVSNFQPHHLQDIIDDTGVVPAVDQVEFHPLLTQRELLKYAKAHDIQVEAWSPLMQGN 180 + F V ++ +++ L + Sbjct: 135 QRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLAD 194 Query: 181 -LDLPVLKELAGKYGKTPAQIVLRWDLQQGVITIPKSVHAERIVENAGFFDFTLSDEDVK 239 DLP + + Y + + ++ + Sbjct: 195 KHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGA 254 Query: 240 VLEDLNQNHRFGPDPDNFNF 259 ++ R+ + Sbjct: 255 RNTLFSRFTRYSGEQTQKAV 274 >BH2158 [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 322 bits (825), Expect = 4e-88, Method: Compositional matrix adjust. Identities = 19/260 (7%), Positives = 47/260 (18%), Gaps = 1/260 (0%) Query: 1 MPWLGLGVWQAKEGDEVIHAVKTALETGYRSIDTAAGYKNEEGVGQAIRESGVPREELFI 60 Q E D + Q + E+ V Sbjct: 17 GLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKH 76 Query: 61 TTKLWNGDQGYESALKAFEVSRKKLGLDVLDLYLIHWPVAGKYRESWKALVHLQKEGLVK 120 ++ S + + + R L Q + Sbjct: 77 GSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQR 136 Query: 121 SIGVSNFQPHHLQDIIDDTGVVPAVDQVEFHPLLTQRELLKYAKAHDIQVEAWSPLMQGN 180 + D ++ +D + + + + + V + L + Sbjct: 137 PTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKH 196 Query: 181 -LDLPVLKELAGKYGKTPAQIVLRWDLQQGVITIPKSVHAERIVENAGFFDFTLSDEDVK 239 L V + ++ L Q + + + + Sbjct: 197 DLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARN 256 Query: 240 VLEDLNQNHRFGPDPDNFNF 259 L + Sbjct: 257 TLFSRFTRYSGEQTQKAVAA 276 >BS_ytbE [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 316 bits (809), Expect = 3e-86, Method: Compositional matrix adjust. Identities = 14/264 (5%), Positives = 44/264 (16%), Gaps = 5/264 (1%) Query: 1 MPWLGLGVWQAKEGDEVIHAVKTALETGYRSIDTAAGYKNEEGVGQAIRE----SGVPRE 56 Q E D + Q + E + + + Sbjct: 17 GLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKH 76 Query: 57 ELFITTKLWNGDQGYESALKAFEVSRKKLGLDVLDLYLIHWPVAGKYRESWKALVHLQKE 116 + + G + L + L + VH + Sbjct: 77 GSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQR 136 Query: 117 GLVKSIGVSNFQPHHLQDIIDDTGVVPAVDQVEFHPLLTQRELLKYAKAHDIQVEAWSPL 176 + + + + + + A + Sbjct: 137 PTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKH 196 Query: 177 MQGN-LDLPVLKELAGKYGKTPAQIVLRWDLQQGVITIPKSVHAERIVENAGFFDFTLSD 235 + + L + + V +++ + + G + Sbjct: 197 DLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARN 256 Query: 236 EDVKVLEDLNQNHRFGPDPDNFNF 259 + + Sbjct: 257 TLFSRFTRYSGEQTQKAVAAYVDI 280 >BS_yvgN [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 304 bits (778), Expect = 1e-82, Method: Compositional matrix adjust. Identities = 19/260 (7%), Positives = 47/260 (18%), Gaps = 1/260 (0%) Query: 1 MPWLGLGVWQAKEGDEVIHAVKTALETGYRSIDTAAGYKNEEGVGQAIRESGVPREELFI 60 Q E D + Q + E+ V Sbjct: 17 GLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKH 76 Query: 61 TTKLWNGDQGYESALKAFEVSRKKLGLDVLDLYLIHWPVAGKYRESWKALVHLQKEGLVK 120 ++ S + + + R L Q + Sbjct: 77 GSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQR 136 Query: 121 SIGVSNFQPHHLQDIIDDTGVVPAVDQVEFHPLLTQRELLKYAKAHDIQVEAWSPLMQGN 180 + D ++ +D + + + + + V + L + Sbjct: 137 PTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKH 196 Query: 181 -LDLPVLKELAGKYGKTPAQIVLRWDLQQGVITIPKSVHAERIVENAGFFDFTLSDEDVK 239 L V + ++ L Q + + + + Sbjct: 197 DLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARN 256 Query: 240 VLEDLNQNHRFGPDPDNFNF 259 L + Sbjct: 257 TLFSRFTRYSGEQTQKAVAA 276 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.137 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,701,310 Number of extensions: 947733 Number of successful extensions: 8578 Number of sequences better than 1.0e-05: 148 Number of HSP's gapped: 8322 Number of HSP's successfully gapped: 149 Length of query: 259 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 155 Effective length of database: 26,383,695 Effective search space: 4089472725 Effective search space used: 4089472725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)