BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0100.0019.p (204 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yngC [S] COG0586 Uncharacterized membrane-associated protein 249 3e-66 BS_ykoX [S] COG0586 Uncharacterized membrane-associated protein 161 5e-40 lin1535 [S] COG0586 Uncharacterized membrane-associated protein 150 2e-36 L119032 [S] COG0586 Uncharacterized membrane-associated protein 125 4e-29 lin1984 [S] COG0586 Uncharacterized membrane-associated protein 115 4e-26 >BS_yngC [S] COG0586 Uncharacterized membrane-associated protein Length = 198 Score = 249 bits (635), Expect = 3e-66, Method: Compositional matrix adjust. Identities = 115/190 (60%), Positives = 143/190 (75%) Query: 4 ISDLISHLFEWIQSLGYFGIMIGLMIEVIPSEIVLAFGGYLVSQGEINYFGAVLFGTVGG 63 + LIS + W+ ++GY GI IGLMIE+IPSEIVLA+GGY+VS+G I + GA++ G +GG Sbjct: 1 MGSLISEILTWLTNMGYAGIAIGLMIEIIPSEIVLAYGGYMVSEGTIGFIGAIIAGVIGG 60 Query: 64 VIAQIFVYWIGRYGGRPVLEKYGKYIFIKKKHIDHSEEWFNKYGTGVIFTARFIPVVRHA 123 IAQIF+YWIGRYGGRP L+KYGKY+ IKK HID SE WF KYG GV+F+ARFIPVVRHA Sbjct: 61 TIAQIFIYWIGRYGGRPFLDKYGKYLLIKKHHIDMSENWFQKYGAGVVFSARFIPVVRHA 120 Query: 124 ISVPAGISRMPLGKFTLLTTLAVIPWSALFVYLGYTLGDKWETIDEVAAKYTHEXXXXXX 183 IS+PAGI+RMP KF +LT LA+IPWS LFVYLG LG +W+ ++ +A YT Sbjct: 121 ISIPAGIARMPFLKFVVLTVLAIIPWSILFVYLGIQLGSQWDDVENIAGTYTTPIMILAV 180 Query: 184 XXXXXYFLFK 193 YF+ K Sbjct: 181 VVIALYFVIK 190 >BS_ykoX [S] COG0586 Uncharacterized membrane-associated protein Length = 221 Score = 161 bits (408), Expect = 5e-40, Method: Compositional matrix adjust. Identities = 78/174 (44%), Positives = 118/174 (67%) Query: 1 LHVISDLISHLFEWIQSLGYFGIMIGLMIEVIPSEIVLAFGGYLVSQGEINYFGAVLFGT 60 L I +L+ E +SL YFGI + L IE IP+E+VL GY VS+G++ G VL G+ Sbjct: 10 LVAIEELVMSWIEAFKSLSYFGIFLALSIEFIPAEVVLPLAGYWVSKGDMTLAGVVLAGS 69 Query: 61 VGGVIAQIFVYWIGRYGGRPVLEKYGKYIFIKKKHIDHSEEWFNKYGTGVIFTARFIPVV 120 +GGV + +YWIGRYGGRP LE++GKY+FIK + +D S+ +F K+G V F+ RF+P + Sbjct: 70 LGGVAGPLTLYWIGRYGGRPFLERFGKYLFIKPEALDKSDNFFKKHGGFVAFSGRFLPGI 129 Query: 121 RHAISVPAGISRMPLGKFTLLTTLAVIPWSALFVYLGYTLGDKWETIDEVAAKY 174 R IS+P GI++M + F+L T +A++P + ++VYLG LG+ W+ + + +Y Sbjct: 130 RTLISIPCGIAKMNVWVFSLYTFIAMLPITFVYVYLGVKLGENWKAVGSILDQY 183 >lin1535 [S] COG0586 Uncharacterized membrane-associated protein Length = 208 Score = 150 bits (378), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 70/181 (38%), Positives = 114/181 (62%), Gaps = 9/181 (4%) Query: 1 LHVISDLISHLFEW-IQSLGYFGIMIGLMIEVI----PSEIVLAFGGYLVSQGEINYFGA 55 LH + + W + SLG++GI +G++IE + PSE+++ FGG++ G++N++ Sbjct: 2 LHTLIQSLQDFTMWLVDSLGHWGIFLGMLIESVCIPLPSEVIMLFGGFMAEAGKLNFWLV 61 Query: 56 VLFGTVGGVIAQIFVYWIGRYGGRPVLEKYGKYIFIKKKHIDHSEEWFNKYGTGVIFTAR 115 V G G ++ + Y+IG++GGR ++ K+GKYIF+ KH+D +E WF +YG +F R Sbjct: 62 VFAGIAGNLVGSLIAYYIGKFGGRALVLKFGKYIFLNVKHLDKAELWFGRYGARAVFFGR 121 Query: 116 FIPVVRHAISVPAGISRMPLGKFTLLTTLAVIPWSALFVYLGYTLGDKWETIDEVAAKYT 175 +PV+R IS+PAGI++M + KF + T L IPW+ +LGY+LG W ++ KYT Sbjct: 122 VLPVIRTFISLPAGIAKMNVWKFVIYTILGCIPWNIFLTWLGYSLGSNWSVVE----KYT 177 Query: 176 H 176 Sbjct: 178 R 178 >L119032 [S] COG0586 Uncharacterized membrane-associated protein Length = 214 Score = 125 bits (314), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 65/175 (37%), Positives = 100/175 (57%), Gaps = 9/175 (5%) Query: 11 LFEWIQSLGYFGIMIGLMIE----VIPSEIVLAFGGYLVSQGEINYFGAVLFGTVGGVIA 66 + + + GYFG+ +MIE IPSE++L FGG++ + E+ G ++ T+G V+ Sbjct: 5 IIQVMNQFGYFGVAFLIMIENIFPPIPSEVILTFGGFMTTYSELGIIGMIIAATIGSVLG 64 Query: 67 QIFVYWIGRYGGRPVLEKY-----GKYIFIKKKHIDHSEEWFNKYGTGVIFTARFIPVVR 121 + +Y++GR LEK GK + +K + I +E+WF K G IF RFIP++R Sbjct: 65 ALILYFVGRLLSVERLEKLVSGRLGKVLRLKPEDITKAEKWFLKRGYATIFFCRFIPLIR 124 Query: 122 HAISVPAGISRMPLGKFTLLTTLAVIPWSALFVYLGYTLGDKWETIDEVAAKYTH 176 IS+PAG ++M L F +LTTL + W+ + V LG LGD WE I + Y+ Sbjct: 125 SLISIPAGSAKMKLPSFLILTTLGTLIWNIVLVCLGAALGDNWEMIAGILDSYSS 179 >lin1984 [S] COG0586 Uncharacterized membrane-associated protein Length = 207 Score = 115 bits (289), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 59/163 (36%), Positives = 97/163 (59%), Gaps = 12/163 (7%) Query: 14 WIQSL----GYFGIMIGLMIEV----IPSEIVLAFGGYLVSQGEINYFGAVLFGTVGGVI 65 WI S+ GY GI + +MIE IPSEI+L FGG++ + +N ++ T+G V+ Sbjct: 4 WITSIMADFGYIGIFLLIMIENLFPPIPSEIILTFGGFMTTVSSLNVVMVIIVATLGSVV 63 Query: 66 AQIFVYWIGRYGGRPVLE----KYGKYIFIKKKHIDHSEEWFNKYGTGVIFTARFIPVVR 121 I +Y + Y G+ L KYG+ + +K+ I+ +E +F KYG+ +F R IP++R Sbjct: 64 GAILLYKVASYFGKERLTHIVLKYGRVLRLKESDIERAESFFLKYGSWAVFLCRMIPLIR 123 Query: 122 HAISVPAGISRMPLGKFTLLTTLAVIPWSALFVYLGYTLGDKW 164 IS+PAG+++M + +F +LTT + W+ + + LG LG+ W Sbjct: 124 SLISIPAGMTKMKMSRFLILTTAGSLLWNTVLIGLGALLGESW 166 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.145 0.461 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,868,798 Number of extensions: 764400 Number of successful extensions: 2811 Number of sequences better than 1.0e-05: 75 Number of HSP's gapped: 2781 Number of HSP's successfully gapped: 75 Length of query: 204 Length of database: 38,378,431 Length adjustment: 101 Effective length of query: 103 Effective length of database: 26,729,697 Effective search space: 2753158791 Effective search space used: 2753158791 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 113 (48.1 bits)