BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0100.0017.p (209 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2820 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 166 3e-41 BS_yqgX [R] COG0491 Zn-dependent hydrolases, including glyoxylases 150 2e-36 lin2270 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 147 9e-36 CAC2272 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 147 1e-35 SA1375 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 144 1e-34 >BH2820 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 211 Score = 166 bits (419), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 93/204 (45%), Positives = 125/204 (61%), Gaps = 8/204 (3%) Query: 9 LGPLQTNAYLLTGADPGRGVIIDPGANPAALLRAAQEMEIE--AVLLTHAHFDHIAGLDE 66 LGPLQTN Y+ T D G G+I DPG L+ ++ +I A+LLTHAHFDHI +++ Sbjct: 8 LGPLQTNWYIQTN-DQGEGIIFDPGGEVEKLITWLRDRQITPLAILLTHAHFDHIGAVED 66 Query: 67 IRRAKKCPVYLHTLESEWLGSPKLNGS-LMWPDVTPPISTEPAEYDLADGQVLNLLGLTF 125 +R PVY+H E EWL P+ NGS L P + I AE+ + Q L++ ++ Sbjct: 67 VRNTFHIPVYIHENEKEWLIDPQRNGSSLFIPGSS--IKAREAEHLITGEQDLSIGSFSY 124 Query: 126 RVMHTPGHSPGSVSFLCGSD--LFSGDVLFKLGVGRTDLTGGRERDLIDSIRNKLYRLDE 183 +V+ TPGHSPGS+S+ D +FSGD LF +GRTDL GG + L+DSI +KL L E Sbjct: 125 QVLETPGHSPGSLSYYAKEDKIVFSGDALFAGSIGRTDLPGGDHQLLLDSIHDKLLELPE 184 Query: 184 EVRVFPGHGPRTTIGFERQCNPYV 207 + V GHGP TTIG E NP++ Sbjct: 185 DTTVASGHGPTTTIGHEMDGNPFL 208 >BS_yqgX [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 211 Score = 150 bits (378), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 88/210 (41%), Positives = 118/210 (56%), Gaps = 8/210 (3%) Query: 2 LNIRSYNLGPLQTNAYLLTGADPGRGVIIDPGANPAALLRAAQEMEIE--AVLLTHAHFD 59 + R +GP+Q NAY L D + +I DPG + + +E + A+LLTHAHFD Sbjct: 1 MKWRRMPVGPIQANAYFLISDD--QCLIFDPGGEGHKINQYIKEKGLTPLAILLTHAHFD 58 Query: 60 HIAGLDEIRRAKKCPVYLHTLESEWLGSPKLNGSLMWPDVTPPISTEPAEYDLADGQVLN 119 HI LDE+R PVYLH E WL LNGS M + ++ +PA++ + LN Sbjct: 59 HIGALDEVREKWDIPVYLHQNEKNWLADASLNGSGMLRGIE--VTAKPADHLIEGDGELN 116 Query: 120 LLGLTFRVMHTPGHSPGSVSFLCG-SDL-FSGDVLFKLGVGRTDLTGGRERDLIDSIRNK 177 + + TPGHSPGSVS+ +DL SGDVLF+ G+GRTDL GG + L+ SI K Sbjct: 117 IGPFHLETLFTPGHSPGSVSYYVKDADLVISGDVLFQGGIGRTDLIGGNQETLLTSIHEK 176 Query: 178 LYRLDEEVRVFPGHGPRTTIGFERQCNPYV 207 L L E V GHGP T + E+ NP++ Sbjct: 177 LLTLPEHTLVLSGHGPETDVLTEQDQNPFL 206 >lin2270 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 205 Score = 147 bits (372), Expect = 9e-36, Method: Compositional matrix adjust. Identities = 87/208 (41%), Positives = 117/208 (56%), Gaps = 7/208 (3%) Query: 1 MLNIRSYNLGPLQTNAYLLTGADPGRGVIIDPG--ANPAALLRAAQEMEIEAVLLTHAHF 58 M I+ G +Q N Y++ +I+DPG AN A+ ++ AVLLTH H+ Sbjct: 1 MTKIKRIMTGIIQENCYIIY--QDNLALIVDPGDEANKIEAEIASLGVKPIAVLLTHCHY 58 Query: 59 DHIAGLDEIRRAKKCPVYLHTLESEWLGSPKLNGSLMWPDVTPPISTEPAEYDLADGQVL 118 DHI L+EIR + PVY+ LE EWL +P LN S I +PAEY+ G Sbjct: 59 DHIGALEEIRSTYQIPVYVSPLEQEWLSNPDLNLSAHI--AGKAIIAQPAEYEFTLGD-Y 115 Query: 119 NLLGLTFRVMHTPGHSPGSVSFLCGSDLFSGDVLFKLGVGRTDLTGGRERDLIDSIRNKL 178 N+ G F+V+ TPGHS GSVSF+ GD LFK +GRTDL G LI+SI+ +L Sbjct: 116 NIEGFRFKVVPTPGHSIGSVSFIFDDFAVVGDALFKGSIGRTDLYTGDFDTLINSIKTEL 175 Query: 179 YRLDEEVRVFPGHGPRTTIGFERQCNPY 206 + L ++ + GHG TTIG E++ NPY Sbjct: 176 FVLPDDFAAYSGHGDATTIGHEKKTNPY 203 >CAC2272 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 199 Score = 147 bits (371), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 79/208 (37%), Positives = 116/208 (55%), Gaps = 11/208 (5%) Query: 2 LNIRSYNLGPLQTNAYLLTGADPGRGVIIDPGANPAALLRAAQEME--IEAVLLTHAHFD 59 + I G TN Y+L V+IDPG P A+L A ++ E ++ VLLTH H D Sbjct: 1 MKISVIQAGAYMTNCYILVDEKSNESVVIDPGDAPQAILEAFKDTETNLKFVLLTHGHAD 60 Query: 60 HIAGLDEIRRAKKCPVYLHTLESEWLGSPKLNGSLMWPDVTPPISTEPAEYDLADGQVLN 119 H A + E+++ VY++ + + + NG M+ D +E A + DG L Sbjct: 61 HTAAVAELKKEYNVDVYMNKDDCKLIE----NGEYMFGD-----KSENATKYVDDGDELQ 111 Query: 120 LLGLTFRVMHTPGHSPGSVSFLCGSDLFSGDVLFKLGVGRTDLTGGRERDLIDSIRNKLY 179 L + + TPGH+PG + +L + LFSGD LF +GRTD GG LI+SI++KL Sbjct: 112 FGELKIKCIKTPGHTPGGMCYLVDNYLFSGDTLFNSSIGRTDFPGGDFSTLINSIKSKLM 171 Query: 180 RLDEEVRVFPGHGPRTTIGFERQCNPYV 207 +LDE + V+PGHG TTIG+E+ NP++ Sbjct: 172 KLDENIVVYPGHGEGTTIGYEKIHNPFI 199 >SA1375 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 207 Score = 144 bits (363), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 75/208 (36%), Positives = 118/208 (56%), Gaps = 5/208 (2%) Query: 2 LNIRSYNLGPLQTNAYLLTGADPGRGVIIDPGANPAALLRAAQEME--IEAVLLTHAHFD 59 + I S LG + TN Y + + ++IDP +++ ++ ++A+LLTHAHFD Sbjct: 1 MRISSLTLGLVDTNTYFI--ENDKAVILIDPSGESEKIIKKLNQINKPLKAILLTHAHFD 58 Query: 60 HIAGLDEIRRAKKCPVYLHTLESEWLGSPKLNGSLMWPDVTPPISTEPAEYDLADGQVLN 119 HI +D+I PVY+H E ++L P NG+ + PI T + + Sbjct: 59 HIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPIITSKVTPEKLNEGSTE 118 Query: 120 LLGLTFRVMHTPGHSPGSVSFLCGSDLFSGDVLFKLGVGRTDLTGGRERDLIDSIRNKLY 179 + G F V+HTPGHSPGS++++ GD LF G+GRTDL G L+DSI++K++ Sbjct: 119 IEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTDLYKGDYETLVDSIQDKIF 178 Query: 180 RLDEEVRVFPGHGPRTTIGFERQCNPYV 207 L+ ++ +FPGHGP TT+ + Q NP++ Sbjct: 179 ELEGDLPLFPGHGPYTTVD-DEQLNPFL 205 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.141 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,250,989 Number of extensions: 677152 Number of successful extensions: 1869 Number of sequences better than 1.0e-05: 96 Number of HSP's gapped: 1723 Number of HSP's successfully gapped: 96 Length of query: 209 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 107 Effective length of database: 26,614,363 Effective search space: 2847736841 Effective search space used: 2847736841 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 113 (48.1 bits)