BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0100.0012.p (303 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0290 [V] COG1131 ABC-type multidrug transport system, ATPase c... 263 3e-70 BS_yfiL [V] COG1131 ABC-type multidrug transport system, ATPase ... 178 1e-44 VNG0689G [V] COG1131 ABC-type multidrug transport system, ATPase... 178 1e-44 PH0913 [V] COG1131 ABC-type multidrug transport system, ATPase c... 175 8e-44 DR2316 [V] COG1131 ABC-type multidrug transport system, ATPase c... 171 1e-42 >BH0290 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 301 Score = 263 bits (672), Expect = 3e-70, Method: Compositional matrix adjust. Identities = 121/298 (40%), Positives = 193/298 (64%), Gaps = 2/298 (0%) Query: 4 LTVANLRKVYGGHTPVDGISFDIEAGKCVALLGPNGAGKTTTLRMLAGLLKPTSGQISFE 63 L + ++ K Y T V S D+ +G+CV L+GPNGAGKTT ++M+ +L PT G I Sbjct: 3 LEIKSVTKKYKEKTAVHDFSMDLLSGECVGLIGPNGAGKTTLIKMITNILDPTRGDILLN 62 Query: 64 GGPVGDYRKRIGYLPQSPAFYNWMSGQEYVVFAAKLYGVSSKEAAGLAADVLEHVGLRDA 123 G + + ++ IGYLPQ P FY WM+ E ++F +L G+S K+ ++L VGLR Sbjct: 63 GKRISEMKREIGYLPQYPDFYQWMTASETLLFMGQLSGISKKKLTKSIPEILSKVGLRGE 122 Query: 124 ARRKIGGYSGGMKQRLGLAQALIHRPRLLLLDEPVSALDPIGRREVMELLRGIREDTTVI 183 K+ +SGGMKQRLG+AQAL+H+P +++DEPVSALDPIGRREV+ ++ I++D+T++ Sbjct: 123 ENSKVSTFSGGMKQRLGIAQALLHKPSFIVMDEPVSALDPIGRREVLNIIEEIKKDSTIL 182 Query: 184 FSTHVLHDAEEICDDIILMNHGLIAEHGALSRLRSQYSIPVIRLTTEREEKAQRWLEDLA 243 STH+L DAEEIC+ ++++ +G E + L ++ S IR+ E ++++ W+E + Sbjct: 183 LSTHILADAEEICERLVMIKNGEKIEDTTMMELLNRNSKNKIRV--EITDESKDWIEVVK 240 Query: 244 HRSFVEQSAVSSGSATLNVKNVELARQIILQEAGNLDIPLLQFEAGSSTLEDLFMKVV 301 ++V+ V+ + VKN+EL + ++L A D+ +++FE TLE+LF+K+V Sbjct: 241 KLNYVQDVEVTGNKVNVEVKNIELHKNMLLANALKHDVNIVKFELDHDTLEELFLKLV 298 >BS_yfiL [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 134 Score = 178 bits (451), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 0/308 (0%), Positives = 0/308 (0%), Gaps = 10/308 (3%) Query: 3 LLTVANLRKVYGGHTPVDGISFDIEAGKCVALLGPNGAGKTTTLRMLAGLLKPTSGQISF 62 + T+ K + A T+ ++ + Sbjct: 1 MKTIRYALKKEKEMMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISGSAI 60 Query: 63 EGGPVGD----YRKRIGYLPQSPAFYNWMSGQEYVVFAAKLYGVSSKEAAGLAADVLEHV 118 + R + Y + +S A+ + E Sbjct: 61 KDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQLHEVW 120 Query: 119 GLRDAARRKIGGYSGGMKQRLGLAQALIHRPRLLLLDEPVSALDPIGRREVMELLRGIRE 178 Sbjct: 121 180 Query: 179 D-TTVIFSTHVLHDAEEICDDIILMNHGLIAEHGALSRLRSQY-SIPVIRLTTEREEKAQ 236 Sbjct: 181 - 239 Query: 237 RWLEDLAHRSFVEQSAVSSGSATLNVKNVELARQI--ILQEAGNLDIPLLQFEAGSSTLE 294 Sbjct: 240 - 298 Query: 295 DLFMKVVG 302 Sbjct: 299 306 >VNG0689G [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 287 Score = 178 bits (451), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 106/304 (34%), Positives = 165/304 (54%), Gaps = 20/304 (6%) Query: 3 LLTVANLRKVYGGHTPVDGISFDIEAGKCVALLGPNGAGKTTTLRMLAGLLKPTSGQISF 62 +++V L K YGG T VDG+SFD+ AG+ L+GPNGAGKT+TL+ML GL++P++G I+ Sbjct: 1 MISVQRLEKRYGGVTAVDGVSFDVGAGEVFGLVGPNGAGKTSTLKMLCGLVEPSAGDITV 60 Query: 63 EGGPVGD--YRKRIGYLPQSPAFYNWMSGQEYVVFAAKLYGVSSKEAAGLAADVLEHVGL 120 G GD R+ +G+LP+ Y+ M+ + Y+ F A LY V A L+ + L Sbjct: 61 NGFDAGDTAMRRTLGFLPEESPLYDEMTPRSYLRFFADLYDVDRATANERIEAALDRLDL 120 Query: 121 RDAARRKIGGYSGGMKQRLGLAQALIHRPRLLLLDEPVSALDPIGRREVMELLRGIRED- 179 DA R +G +S GM +++ +A++L++ P +L+ DEP S LDP+ V+E R + D Sbjct: 121 -DARDRPVGDFSKGMTRKVAIARSLVNDPDVLVYDEPASGLDPLTTNTVIEFTRELAADG 179 Query: 180 TTVIFSTHVLHDAEEICDDIILMNHGLIAEHGALSRLRSQYSIPVIRLTTEREEKAQRWL 239 T++FS H LH E +CD + +M G A G L+ +R ++ E + R Sbjct: 180 KTIVFSAHDLHHVESVCDRVAIMRDGQFAATGTLADIRDEHG-----------EVSYRVF 228 Query: 240 EDLAHRSFVEQSAVSSGSATLNVKNVELARQIILQEAGNLDIPLLQFEAGSSTLEDLFMK 299 D+ V+ + S+G V ++ I A ++ STLED+F+ Sbjct: 229 TDVD----VDDARPSNGRYVTTVDSMADVDAIRAAAADAG-GGVVDIRTDESTLEDVFLD 283 Query: 300 VVGA 303 V GA Sbjct: 284 VTGA 287 >PH0913 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 324 Score = 175 bits (443), Expect = 8e-44, Method: Compositional matrix adjust. Identities = 103/306 (33%), Positives = 164/306 (53%), Gaps = 15/306 (4%) Query: 4 LTVANLRKVYGGHTPVDGISFDIEAGKCVALLGPNGAGKTTTLRMLAGLLKPTSGQISFE 63 + V NL K YG V GISF + G+ A LGPNGAGKTTT+ ML LLKPTSG+ Sbjct: 7 IIVENLVKKYGDFEAVRGISFKVRRGEIFAFLGPNGAGKTTTVHMLTTLLKPTSGRAIVA 66 Query: 64 GGPV----GDYRKRIGYLPQSPAFYNWMSGQEYVVFAAKLYGVSSKEAAGLAADVLEHVG 119 G V + RK+IG + Q P+ ++ E + ++YG+ +E ++L+ V Sbjct: 67 GHDVVKESREVRKKIGVVFQDPSVDRELTAWENMYIHGRIYGLKGQELKKKIEELLKFVE 126 Query: 120 LRDAARRKIGGYSGGMKQRLGLAQALIHRPRLLLLDEPVSALDPIGRREVMELLRGIRE- 178 L + R++ +SGGM++RL +A+ALIH P +L LDEP LDP R + E ++ ++E Sbjct: 127 LWEFKDRQVKYFSGGMQRRLEIARALIHEPEVLFLDEPTIGLDPQTRAHIWEYIKTMKEE 186 Query: 179 -DTTVIFSTHVLHDAEEICDDIILMNHGLIAEHGALSRLRSQYSIPVIRLTTEREEKAQR 237 D T+ +TH + +AE++ D I +++HG I G L+ +I + E+ Q Sbjct: 187 HDMTIFLTTHYMDEAEQLADRIAIIDHGKIIAEGTAEELKKLVGNDIIYVKFEKPTCIQ- 245 Query: 238 WLEDLAHRSFVEQSAV-SSGSATLNVKNVELARQIILQEAGNLDIPLLQFEAGSSTLEDL 296 F+ V ++G L VKN A ++ ++A +++ +L+ TL D+ Sbjct: 246 -------AEFIRGCKVLNNGRVALEVKNAAEALPLVFEKAREMELKILEVTYHRPTLNDV 298 Query: 297 FMKVVG 302 F+ + G Sbjct: 299 FLHLTG 304 >DR2316 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 327 Score = 171 bits (433), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 95/231 (41%), Positives = 137/231 (59%), Gaps = 10/231 (4%) Query: 8 NLRKVYGGHTPVDGISFDIEAGKCVALLGPNGAGKTTTLRMLAGLLKPTSGQISFEGGPV 67 LRKVY VDG++ + AG+ LGPNGAGK+TT++ML GL+ P+ G+ G Sbjct: 20 ELRKVYRDRAVVDGLTLTVGAGEVFGFLGPNGAGKSTTVKMLLGLVIPSGGEARVLCGSP 79 Query: 68 GD--YRKRIGYLPQSPAFYNWMSGQEYVVFAAKLYGVSSKEAAGLAADVLEHVGLRDAAR 125 D R R+G+LP+ F WM+ +E++ F +L G+ S E VLE VGL Sbjct: 80 ADPAVRARLGFLPEQFRFQLWMTAEEFLNFHGQLAGMKSNELRTRVPQVLEEVGLGGRGG 139 Query: 126 RKIGGYSGGMKQRLGLAQALIHRPRLLLLDEPVSALDPIGR---REVMELLRGIREDTTV 182 ++ GYS GM QR GLA A++ RP+L+ LDEP SALDPIGR RE++E L+G E V Sbjct: 140 EQLSGYSKGMLQRCGLAGAILARPQLVFLDEPTSALDPIGRVEVREIIERLKG--EGVAV 197 Query: 183 IFSTHVLHDAEEICDDIILMNHGLIAEHGALSRLRS---QYSIPVIRLTTE 230 ++H+L + E++CD + + G + + G++ L S+ V RLT+E Sbjct: 198 FLNSHLLSEVEQVCDRVAFVKAGRVLQQGSMRELMGGVIPVSVRVDRLTSE 248 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,348,650 Number of extensions: 913466 Number of successful extensions: 10074 Number of sequences better than 1.0e-05: 2192 Number of HSP's gapped: 7063 Number of HSP's successfully gapped: 2419 Length of query: 303 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 197 Effective length of database: 26,153,027 Effective search space: 5152146319 Effective search space used: 5152146319 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)