BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0100.0008.p (472 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin1867 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 600 e-171 L0473 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A sub... 524 e-148 all1053 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 524 e-148 slr0877 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 514 e-146 SPy1771 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 508 e-144 >lin1867 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 301 Score = 600 bits (1546), Expect = e-171, Method: Compositional matrix adjust. Identities = 0/471 (0%), Positives = 0/471 (0%), Gaps = 2/471 (0%) Query: 2 LRSKEISVGELTEESLAAIAERDGKVHAFLTLNEEGARASARALDDKLASGAARGLLFGL 61 R L + I+ K A + Sbjct: 15 GRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIY--VDTPGLHMEEKR 72 Query: 62 PAGIKDNIVTKGLRTTCASQFLSNFQPIYDATVVTKLRQADAVTVGKLNMDEFAMGGSNE 121 N + L + + + Sbjct: 73 AINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILAVNKVDNVQEK 132 Query: 122 NSSFGPVRNPWDVERVPXXXXXXXXXXXXXXEVLFTLGSDTGGSIRQPASYCGVVGLKPT 181 ++ + + + + Sbjct: 133 ADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHFPEDYITDRSQRF 192 Query: 182 YGLVSRYGLVAFASSLDQIGPITRNVEDSAYVLQAIAGYDAQDSTSANVDIPDYLSALTG 241 + + +T +E + + + Sbjct: 193 MASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDINGLILVEREGQKKMVIGNKGA 252 Query: 242 DISGLRIAVPKEYIGEGVDASVRDTVLSALKVLESLGAVWEEVSLPHTEYAVATYYXXXX 301 Sbjct: 253 312 Query: 302 XXXXXXXXRFDGVRYGVRADDGGGLLDLYHNSRSEGFGAEVKRRIMLGTYALSSGYYDAY 361 Sbjct: 313 372 Query: 362 YLKAQKVRTLIKQDFDDVFQKYDVVIGPTAPTTAFKLGSQIEDPLTMYLNDILTIPVSLA 421 Sbjct: 373 432 Query: 422 GIPAVSIPCGFAEGLPVGLQIIGKEFDESTVLRVAHAFEQHTDHHKQLPQL 472 Sbjct: 433 483 >L0473 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 486 Score = 524 bits (1350), Expect = e-148, Method: Compositional matrix adjust. Identities = 257/475 (54%), Positives = 339/475 (71%), Gaps = 12/475 (2%) Query: 1 MLRSKEISVGELTEESLAAIAERDGKVHAFLTLNEEGARASARALDDKLASGAARGL--- 57 +L +KEIS ELT+ +L+ I+ R+ ++ AFL + EE A A A+D ARG+ Sbjct: 14 LLVNKEISALELTKATLSDISAREPQIDAFLKITEEKALQDAAAID-------ARGINPD 66 Query: 58 --LFGLPAGIKDNIVTKGLRTTCASQFLSNFQPIYDATVVTKLRQADAVTVGKLNMDEFA 115 G+ +KDNIVT+G+ TT AS+ L + P Y+ATV KL ++ +T+GKLNMDEFA Sbjct: 67 VVTDGISIAVKDNIVTEGIETTAASKILGGWIPPYNATVANKLSESGLITIGKLNMDEFA 126 Query: 116 MGGSNENSSFGPVRNPWDVERVPXXXXXXXXXXXXXXEVLFTLGSDTGGSIRQPASYCGV 175 MGGS ENSS P +N WD +VP +V +LGSDTGGSIRQPA++ G+ Sbjct: 127 MGGSGENSSVKPTKNAWDQTKVPGGSSSGSAASVASGQVRLSLGSDTGGSIRQPAAFNGI 186 Query: 176 VGLKPTYGLVSRYGLVAFASSLDQIGPITRNVEDSAYVLQAIAGYDAQDSTSANVDIPDY 235 VGLKPTYG VSR+GL+AFASSLDQIGP+ VE++A +L I+G+D DSTS+NV +PD+ Sbjct: 187 VGLKPTYGRVSRFGLIAFASSLDQIGPLAPTVEENAQLLNVISGFDKNDSTSSNVAVPDF 246 Query: 236 LSALTGDISGLRIAVPKEYIGEGVDASVRDTVLSALKVLESLGAVWEEVSLPHTEYAVAT 295 S + DI G++IA+PKEY GEG+D V++ +L+A K LE LGA+ EEVSLPH++Y VA Sbjct: 247 TSKIGQDIKGMKIALPKEYFGEGIDEKVKEQILAAAKHLEKLGAIVEEVSLPHSKYGVAV 306 Query: 296 YYXXXXXXXXXXXXRFDGVRYGVRADDGGGLLDLYHNSRSEGFGAEVKRRIMLGTYALSS 355 YY RFDG+RYG RA D L DLY SRSEGFG EV+RRIMLGT++LS+ Sbjct: 307 YYIIASSEASSNLQRFDGIRYGYRAQDIKNLEDLYVKSRSEGFGPEVQRRIMLGTFSLSA 366 Query: 356 GYYDAYYLKAQKVRTLIKQDFDDVFQKYDVVIGPTAPTTAFKLGSQIEDPLTMYLNDILT 415 G YD ++ KA +VRTLI DF VF+KYD+++GPT PT A+ LG++++DPL+MYL D+LT Sbjct: 367 GSYDKHFKKAGQVRTLIINDFAKVFEKYDLILGPTTPTPAWDLGARVDDPLSMYLADLLT 426 Query: 416 IPVSLAGIPAVSIPCGFAEGLPVGLQIIGKEFDESTVLRVAHAFEQHTDHHKQLP 470 IPV+LAG+P +SIP GFA+GLPVG+Q+IGK +DE T+ +VA AFE TD HK+ P Sbjct: 427 IPVNLAGLPGISIPAGFADGLPVGMQLIGKRYDEETIYKVAAAFEATTDFHKKQP 481 >all1053 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 486 Score = 524 bits (1350), Expect = e-148, Method: Compositional matrix adjust. Identities = 265/475 (55%), Positives = 340/475 (71%), Gaps = 4/475 (0%) Query: 2 LRSKEISVGELTEESLAAIAERDGKVHAFLTLNEEGARASARALDDKLASGAARGLLFGL 61 L KE S E+T+E+L I E + K+H+FL + + A ARA+D K+A+G GLL G+ Sbjct: 11 LVKKERSAVEITQETLDHIQELEPKLHSFLHITAQQALEQARAVDAKIAAGEEIGLLAGI 70 Query: 62 PAGIKDNIVTKGLRTTCASQFLSNFQPIYDATVVTKLRQADAVTVGKLNMDEFAMGGSNE 121 P G+KDN+ TKG+ TTCAS+ L NF P Y++TV KL A AV VGK N+DEFAMG S E Sbjct: 71 PIGVKDNMCTKGIPTTCASRILENFVPPYESTVTQKLLDAGAVVVGKTNLDEFAMGSSTE 130 Query: 122 NSSFGPVRNPWDVERVPXXXXXXXXXXXXXXEVLFTLGSDTGGSIRQPASYCGVVGLKPT 181 NS++ NPWD+ RVP E + LGSDTGGSIRQPAS+CGVVGLKPT Sbjct: 131 NSAYQVTANPWDLSRVPGGSSGGSAAAVAAEECVVALGSDTGGSIRQPASFCGVVGLKPT 190 Query: 182 YGLVSRYGLVAFASSLDQIGPITRNVEDSAYVLQAIAGYDAQDSTSANVDIPDYLSALTG 241 YGLVSRYGLVA+ASSLDQIGP ++VED+A +L+AIAGYD QDSTS V+IPDY+++L Sbjct: 191 YGLVSRYGLVAYASSLDQIGPFGKSVEDTAILLKAIAGYDHQDSTSLKVEIPDYVASLKP 250 Query: 242 DI---SGLRIAVPKEYIGEGVDASVRDTVLSALKVLESLGAVWEEVSLPHTEYAVATYYX 298 D+ S LRI + KE GEG+D+ V V A++ L+ LGA +S P+ Y V +YY Sbjct: 251 DLKARSKLRIGIIKETFGEGLDSVVEQAVTKAIEQLQRLGAEVHVISCPNFRYGVPSYYI 310 Query: 299 XXXXXXXXXXXRFDGVRYGVRADDGGGLLDLYHNSRSEGFGAEVKRRIMLGTYALSSGYY 358 R+DGV+YG RA +G LL +Y +R+ GFGAEVKRRIM+GTYALS+GYY Sbjct: 311 IAPSEASANLARYDGVKYGWRAPEGDNLLSMYKRTRATGFGAEVKRRIMIGTYALSAGYY 370 Query: 359 DAYYLKAQKVRTLIKQDFDDVFQKYDVVIGPTAPTTAFKLGSQIEDPLTMYLNDILTIPV 418 DAYYLKAQKVRTLIKQDF++ F+ DV++ PTAPTTAFK G + DP++MYLND++TIPV Sbjct: 371 DAYYLKAQKVRTLIKQDFENAFKNVDVLVSPTAPTTAFKAGEKTADPISMYLNDLMTIPV 430 Query: 419 SLAGIPAVSIPCGFAE-GLPVGLQIIGKEFDESTVLRVAHAFEQHTDHHKQLPQL 472 +LAG+P +S+PCGF E GLP+GLQ+IGK E +L++A+A+EQ T H P++ Sbjct: 431 NLAGLPGLSVPCGFDEQGLPIGLQLIGKVLREDQLLQIAYAYEQSTSWHLSQPKI 485 >slr0877 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 483 Score = 514 bits (1325), Expect = e-146, Method: Compositional matrix adjust. Identities = 256/472 (54%), Positives = 329/472 (69%), Gaps = 2/472 (0%) Query: 2 LRSKEISVGELTEESLAAIAERDGKVHAFLTLNEEGARASARALDDKLASGAARGLLFGL 61 L +EIS + + SLA I D ++ +FL + A A A +D ++A+G GLL G+ Sbjct: 11 LIDQEISAVAVAQASLARIEAVDDRLKSFLQVTAPQAIAQAEKVDAQIAAGEPIGLLAGI 70 Query: 62 PAGIKDNIVTKGLRTTCASQFLSNFQPIYDATVVTKLRQADAVTVGKLNMDEFAMGGSNE 121 P GIKDN+ TKG+ TTCASQ L F P Y++TV KL++A AV VGK N+DEFAMG S E Sbjct: 71 PIGIKDNLCTKGIVTTCASQILRGFVPPYESTVTEKLQKAGAVMVGKTNLDEFAMGSSTE 130 Query: 122 NSSFGPVRNPWDVERVPXXXXXXXXXXXXXXEVLFTLGSDTGGSIRQPASYCGVVGLKPT 181 NS + NPWD+ RVP E L LGSDTGGSIRQPAS CGVVG+KPT Sbjct: 131 NSGYQVTANPWDLTRVPGGSSGGSAAAVAADECLIALGSDTGGSIRQPASLCGVVGMKPT 190 Query: 182 YGLVSRYGLVAFASSLDQIGPITRNVEDSAYVLQAIAGYDAQDSTSANVDIPDYLSALTG 241 YGLVSR+GLVA+ASSLDQIGP R VED+A +LQAIAG+D++DSTS +V IPDY AL Sbjct: 191 YGLVSRFGLVAYASSLDQIGPFARRVEDAAILLQAIAGHDSRDSTSLDVPIPDYTQALKP 250 Query: 242 DISGLRIAVPKEYIGEGVDASVRDTVLSALKVLESLGAVWEEVSLPHTEYAVATYYXXXX 301 D+ G+++ V + GEG+D +V + V +A+ L LGA EE+ P +A YY Sbjct: 251 DLKGVKVGVIMDAFGEGLDDTVNEAVQTAIAKLRELGATIEEIHCPRFRSGIAAYYVIAP 310 Query: 302 XXXXXXXXRFDGVRYGVRADDGGGLLDLYHNSRSEGFGAEVKRRIMLGTYALSSGYYDAY 361 R+D VRYG+RA + L+++Y ++R++GFGAEVKRRIMLGTYALS+GYYDAY Sbjct: 311 SEASANLARYDAVRYGLRA-EADNLMEMYTHTRAKGFGAEVKRRIMLGTYALSAGYYDAY 369 Query: 362 YLKAQKVRTLIKQDFDDVFQKYDVVIGPTAPTTAFKLGSQIEDPLTMYLNDILTIPVSLA 421 YLKAQKVRTLIKQDFD F K DV++ PTAPTTAFK G + +DPL+MYL+D++TIPV+LA Sbjct: 370 YLKAQKVRTLIKQDFDQAFTKVDVLVCPTAPTTAFKAGEKTDDPLSMYLSDLMTIPVNLA 429 Query: 422 GIPAVSIPCGFAE-GLPVGLQIIGKEFDESTVLRVAHAFEQHTDHHKQLPQL 472 G+PA+S+PCGF + LP+GLQ++G E + RV +A+EQ T H + P L Sbjct: 430 GLPAMSVPCGFDDNNLPIGLQLVGNVLGEEMLFRVGYAYEQATTWHDRQPDL 481 >SPy1771 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 488 Score = 508 bits (1309), Expect = e-144, Method: Compositional matrix adjust. Identities = 252/470 (53%), Positives = 326/470 (69%), Gaps = 2/470 (0%) Query: 1 MLRSKEISVGELTEESLAAIAERDGKVHAFLTLNEEGARASARALDDKLASGAARGLLFG 60 +L +KEIS ELT+ +L I R+ V +F+T++EE A A A+D K A L+ G Sbjct: 14 LLVAKEISATELTQATLEDIKSREEAVGSFITVSEEVALKQAAAIDAKGID--ADNLMSG 71 Query: 61 LPAGIKDNIVTKGLRTTCASQFLSNFQPIYDATVVTKLRQADAVTVGKLNMDEFAMGGSN 120 +P +KDNI TK + TT AS+ L N++PI++AT V D + +GK NMDEFAMGGS Sbjct: 72 IPLAVKDNISTKEILTTAASKMLYNYEPIFNATSVANAYAKDMIVIGKTNMDEFAMGGST 131 Query: 121 ENSSFGPVRNPWDVERVPXXXXXXXXXXXXXXEVLFTLGSDTGGSIRQPASYCGVVGLKP 180 E S F +N WD +VP +V +LGSDTGGSIRQPA++ VVGLKP Sbjct: 132 ETSYFKKTKNAWDHTKVPGGSSGGSATAVASGQVRLSLGSDTGGSIRQPAAFNSVVGLKP 191 Query: 181 TYGLVSRYGLVAFASSLDQIGPITRNVEDSAYVLQAIAGYDAQDSTSANVDIPDYLSALT 240 TYG VSRYGL+AF SSLDQIGP V+++A +L IA D +D+TSA V I DY S + Sbjct: 192 TYGTVSRYGLIAFGSSLDQIGPFAPTVKENAQLLNVIASSDVKDATSAPVRIADYTSKIG 251 Query: 241 GDISGLRIAVPKEYIGEGVDASVRDTVLSALKVLESLGAVWEEVSLPHTEYAVATYYXXX 300 DI G++IA+PKEY+GEG+D +++TVL+++K E+LGA EEVSLPH++Y VA YY Sbjct: 252 RDIKGMKIALPKEYLGEGIDPEIKETVLASVKQFEALGATVEEVSLPHSKYGVAVYYIIA 311 Query: 301 XXXXXXXXXRFDGVRYGVRADDGGGLLDLYHNSRSEGFGAEVKRRIMLGTYALSSGYYDA 360 RFDG+RYG RADD L ++Y N+RS+GFG EVKRRIMLGT++LSSGYYDA Sbjct: 312 SSEASSNLQRFDGIRYGFRADDAKNLDEIYVNTRSQGFGDEVKRRIMLGTFSLSSGYYDA 371 Query: 361 YYLKAQKVRTLIKQDFDDVFQKYDVVIGPTAPTTAFKLGSQIEDPLTMYLNDILTIPVSL 420 Y+ KA +VRTLI QDFD VF YD+++GPT PT AF L + DP+ MYL D+LTIPV+L Sbjct: 372 YFKKAGQVRTLIIQDFDKVFADYDLILGPTTPTVAFGLDTLNHDPVAMYLADLLTIPVNL 431 Query: 421 AGIPAVSIPCGFAEGLPVGLQIIGKEFDESTVLRVAHAFEQHTDHHKQLP 470 AG+P +SIP GF +GLPVGLQ+IG ++ E T+ + A AFE TD+HKQ P Sbjct: 432 AGLPGISIPAGFVDGLPVGLQLIGPKYAEETIYQAAAAFEAVTDYHKQQP 481 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.136 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,045,394 Number of extensions: 1307544 Number of successful extensions: 3133 Number of sequences better than 1.0e-05: 78 Number of HSP's gapped: 2936 Number of HSP's successfully gapped: 89 Length of query: 472 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 362 Effective length of database: 25,691,691 Effective search space: 9300392142 Effective search space used: 9300392142 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 118 (50.1 bits)