BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0100.0003.p (579 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC1556 [R] COG3858 Predicted glycosyl hydrolase 105 2e-22 BS_yvbX [R] COG3858 Predicted glycosyl hydrolase 104 5e-22 BS_ydhD [R] COG3858 Predicted glycosyl hydrolase 103 9e-22 BS_ykvQ [R] COG3858 Predicted glycosyl hydrolase 100 5e-21 BS_yaaH [R] COG3858 Predicted glycosyl hydrolase 92 3e-18 >CAC1556 [R] COG3858 Predicted glycosyl hydrolase Length = 446 Score = 105 bits (263), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 75/282 (26%), Positives = 121/282 (42%), Gaps = 17/282 (6%) Query: 299 VDIKGNVRSKADSAYVQWAHQQGMEVWGLLTNSFDADLTTQALSSYEKRMNAIVQMLEYA 358 D GN+ + V +A+ G++ + N+FD + L + R N I +L Sbjct: 172 TDGVGNISGLVPTNQVTYANNNGIKTLATIENNFDGAVAQTLLENSTNRQNLINNILTSL 231 Query: 359 DLYGLDGINIDFENVYTKDGENVTQFMRELKPMAQAKNLIVSMDV---TPKSNSEMWSLF 415 G G+NID E VY D +T FM EL + V++ V T S + W+ Sbjct: 232 KANGYKGVNIDLEGVYYYDRSYLTTFMNELYSTLNPQGFYVTICVPAKTSDSPTAAWNGA 291 Query: 416 LDRRALGAVTDFLIVMAYDEHW-ASSPTAGSVASLPWVEKSVSRILEEDDVPAEKLILGI 474 D AL D +I+M YDEH+ SP G +AS+ WVE + + +P K++LG Sbjct: 292 FDYAALAKAADQVILMTYDEHYGGGSP--GPIASIGWVENVIKYAITV--IPTTKIMLGA 347 Query: 475 PLYTRIWSETTKDGKTKVSSKAVSMNAVQEILAEKKLKPVLDKDSGQNYVEYTE-DGVLR 533 Y WS +KA ++ + + D S Y Y + G+ Sbjct: 348 AAYGYDWSSN--------PTKAYGISGTYNLASTYNATINWDLTSQSPYFYYVDSSGINH 399 Query: 534 KIWIEDKVSLKARVELAKSFGLGGIGSWNRSFAVPEAWETLQ 575 +W E+ SL +++L S+ L G+ W + W +++ Sbjct: 400 SVWFENGQSLNYKLDLINSYNLSGVAIWRLGLENSDYWTSIR 441 >BS_yvbX [R] COG3858 Predicted glycosyl hydrolase Length = 344 Score = 104 bits (259), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 75/289 (25%), Positives = 140/289 (48%), Gaps = 22/289 (7%) Query: 303 GNVRSKADSAYVQWAHQQGMEVWGLLTN------SFDADLTTQALSSYEKRMNAIVQMLE 356 G + A + + +A ++ ++ W +++N FD DL ++ +S+ + Q++ Sbjct: 66 GQIIGDAPTKQLTYAKKKKIKTWAVISNYNDAIYDFDGDLASRVMSNKTAKKRFTDQLIT 125 Query: 357 YADLYGLDGINIDFENVYTKDGENVTQFMRELKPMAQAKNLIVSMDVTPKS---NSEMWS 413 A + GINIDFE V +D + F++ + K++ + V KS ++ WS Sbjct: 126 LAKKHSYYGINIDFEAVNPEDRAAYSNFIQYVSQALNKKHIKTMVSVPAKSADDKNDDWS 185 Query: 414 LFLDRRALGAVTDFLIVMAYDEH--WASSPTAGSVASLPWVEKSVSRILEEDDVPAEKLI 471 D +G DF+ VM YDEH W GSVAS W++ S+ +++ + A K+I Sbjct: 186 WPYDYAKIGKYADFVQVMTYDEHGIWGE---PGSVASTNWIKSSLQFSVKK--IKANKVI 240 Query: 472 LGIPLYTRIWSETTKDGKTKVSSKAVSMNAVQEILAEKKLKPVLDKDSGQNYVEYTEDGV 531 +GIP Y W KDG T S N ++ ++ ++K KP +K SG Y + Sbjct: 241 MGIPAYGYDWD--VKDGST---STIREWNELKSLIKKQKAKPAFNKKSGSMTFSYVDKKK 295 Query: 532 LRK-IWIEDKVSLKARVELAKSFGLGGIGSWNRSFAVPEAWETLQQISK 579 + +W E++ +++ + LAK + + G+ + W+ +++ +K Sbjct: 296 HKHVVWYENEKTVQTKSHLAKQYKIAGVSVYALGNESESFWKAIRKGTK 344 >BS_ydhD [R] COG3858 Predicted glycosyl hydrolase Length = 115 Score = 103 bits (256), Expect = 9e-22, Method: Compositional matrix adjust. Identities = 0/250 (0%), Positives = 0/250 (0%), Gaps = 10/250 (4%) Query: 324 VWGLLTNSFDADLTTQALSSYEKRMNAIVQMLEYADLYGLDGINIDFENVYTKDGENVTQ 383 Sbjct: 165 224 Query: 384 FMRELKPMAQAKNLIVSMDVTPKSNSEM-WSLFLDRRALGAVTDFLIVMAYDEHWASSPT 442 Sbjct: 225 - 283 Query: 443 AGSVASLPWVEKSVSRILEEDDVPAEKLILGIPLYTRIWSETTKDGKTKVSSKAVSMNAV 502 Sbjct: 284 ---- 339 Query: 503 QEILAEKKLKPVLDKDSGQN-YVEYT-EDGVLRKIWIEDKVSLKARVELAKSFGLGGIGS 560 Sbjct: 340 -- 397 Query: 561 WNRSFAVPEA 570 Sbjct: 398 407 >BS_ykvQ [R] COG3858 Predicted glycosyl hydrolase Length = 232 Score = 100 bits (250), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 69/197 (35%), Positives = 103/197 (52%), Gaps = 9/197 (4%) Query: 288 VNVVSPTWFEIVDIKGNVRSKADSAYVQWAHQQGMEVWGLLTNSFDADLTTQALSSYEKR 347 +NVVSP +F I + A+ ++ H++ ++V L+N +D DL AL + Sbjct: 26 LNVVSPDFFYIRKDGSLLLRSANPDVIKALHRRKLKVVPFLSNHWDQDLGRFALMNRVLL 85 Query: 348 MNAIVQMLEYADLYGLDGINIDFENVYTKDGENVTQFMREL-KPMAQAKNLIVSMDVTPK 406 I +E DL DG+NID EN+ KD T F+R + K + + K + V++ P Sbjct: 86 AKQISHAIEELDL---DGVNIDIENLTEKDRNLFTDFIRLVRKSIPRHKEVSVAVAANPF 142 Query: 407 SNSEMWSLFLDRRALGAVTDFLIVMAYDEHWASSPTAGSVASLPWVEKSVSRILEEDDVP 466 ++ W D L D+L+VMAYDE S AG AS+ WVEKS+ +L + P Sbjct: 143 GWTKGWHGSYDYEYLAKYADYLMVMAYDE----SRLAGPTASVGWVEKSIQYVLTQKISP 198 Query: 467 AEKLILGIPLYTRIWSE 483 +KL+LG+P Y R W E Sbjct: 199 -QKLVLGLPFYGRCWME 214 >BS_yaaH [R] COG3858 Predicted glycosyl hydrolase Length = 188 Score = 91.7 bits (226), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 0/257 (0%), Positives = 0/257 (0%), Gaps = 9/257 (3%) Query: 322 MEVWGLLTNSFDADLTTQALSSYEKRMNAIVQMLEYADLYGLDGINIDFENVYTKDGENV 381 Sbjct: 164 223 Query: 382 TQFMRELKPMAQAKNLIVSMDVTPKSNSEM---WSLFLDRRALGAVTDFLIVMAYDEHWA 438 Sbjct: 224 283 Query: 439 SSPTAGSVASLPWVEKSVSRILEEDDVPAEKLILGIPLYTRIWSETTKDGKTKVSSKAVS 498 Sbjct: 284 ----- 338 Query: 499 MNAVQEILAEKKLKPVLDKDSGQNYVEYTE-DGVLRKIWIEDKVSLKARVELAKSFGLGG 557 Sbjct: 339 398 Query: 558 IGSWNRSFAVPEAWETL 574 Sbjct: 399 415 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.131 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 42,265,410 Number of extensions: 1825399 Number of successful extensions: 2956 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 2934 Number of HSP's successfully gapped: 7 Length of query: 579 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 467 Effective length of database: 25,461,023 Effective search space: 11890297741 Effective search space used: 11890297741 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 119 (50.4 bits)