BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0099.0008.p (308 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPy0898 [K] COG0583 Transcriptional regulator 310 2e-84 SP0676 [K] COG0583 Transcriptional regulator 290 2e-78 L0219 [K] COG0583 Transcriptional regulator 180 3e-45 SP0927 [K] COG0583 Transcriptional regulator 167 1e-41 SPy0824 [K] COG0583 Transcriptional regulator 156 5e-38 >SPy0898 [K] COG0583 Transcriptional regulator Length = 301 Score = 310 bits (794), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 148/297 (49%), Positives = 213/297 (71%) Query: 1 LQQLRYAIEIANSGSMNEAAKRLFVSQPSLSNAIKELENELGITIFERNNRGISISAEGV 60 +QQL Y I+I GSMNEAAK+LF++QPSLSNA+K+LE E+GITIF RN +GI+++ +GV Sbjct: 3 IQQLHYIIKIVECGSMNEAAKQLFITQPSLSNAVKDLEMEMGITIFNRNPKGITLTKDGV 62 Query: 61 EFLGYARQIIEQTEFMENRYTGKKRSPIYFSVSTQHYPFVVDAFVKLMKENKVTEYNFSL 120 EFL YARQIIEQT +E+RY FSVS+QHY FVV+AFV L+K +T Y L Sbjct: 63 EFLSYARQIIEQTSLLEDRYKNHNTGRELFSVSSQHYAFVVNAFVSLLKRTDMTRYELFL 122 Query: 121 RETQTYEIIEDVRTLRSDLGILYINEGNYKVMNKLFSDGNLKFTPLFNTNPHVYVRAGHM 180 RET+T+EII+DV+ RS++G+L+IN+ N V+ KLF D +L +PLF PH++V + Sbjct: 123 RETRTWEIIDDVKNFRSEIGVLFINDYNRDVLTKLFDDNHLTASPLFKAQPHIFVSKSNP 182 Query: 181 LAGKELITLEDILPYPYITFEQGENNSLHFSEEMLSFTQIEKNIQVTDRATLTHLLLGSD 240 LA K L++++D+ +PY++++QG +NS +FSEEM+S K+I V+DRATL +L++G D Sbjct: 183 LATKSLLSMDDLRDFPYLSYDQGIHNSFYFSEEMMSQMPHNKSIVVSDRATLFNLMIGLD 242 Query: 241 SYTVGTGIMDAELNGDGLVTIPFDSPEMFTVGWIAHKDRKPGEMMSAYIDILNDLVT 297 YTV +GI+++ LNGD +V IP D P+ + +I H+ +M +I+ L + VT Sbjct: 243 GYTVASGILNSNLNGDQIVAIPLDVPDEIDIVFIKHEKANLSKMGERFIEYLLEEVT 299 >SP0676 [K] COG0583 Transcriptional regulator Length = 322 Score = 290 bits (743), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 138/292 (47%), Positives = 208/292 (71%) Query: 1 LQQLRYAIEIANSGSMNEAAKRLFVSQPSLSNAIKELENELGITIFERNNRGISISAEGV 60 +QQL Y I+I +GSMNEAAK+LF++QPSLSNA+++LENE+GI IF RN +GI+++ +G+ Sbjct: 23 IQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMGIEIFIRNPKGITLTRDGM 82 Query: 61 EFLGYARQIIEQTEFMENRYTGKKRSPIYFSVSTQHYPFVVDAFVKLMKENKVTEYNFSL 120 EFL YARQ++EQT+ +E RY FSVS+QHY FVV+AFV L+K++ + +Y L Sbjct: 83 EFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFVVNAFVSLLKKSDMEKYELFL 142 Query: 121 RETQTYEIIEDVRTLRSDLGILYINEGNYKVMNKLFSDGNLKFTPLFNTNPHVYVRAGHM 180 RET+T+EII+DV+ RS++G+L++N N V+ K+ D +L LF PH++V + Sbjct: 143 RETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDDNHLLAHHLFTAQPHIFVSKTNP 202 Query: 181 LAGKELITLEDILPYPYITFEQGENNSLHFSEEMLSFTQIEKNIQVTDRATLTHLLLGSD 240 LA K+ + L D+ +PY++++QG +NS +FSEE+LS +K+I V+DRATL +LL+G D Sbjct: 203 LAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILSQEHHKKSIVVSDRATLFNLLIGLD 262 Query: 241 SYTVGTGIMDAELNGDGLVTIPFDSPEMFTVGWIAHKDRKPGEMMSAYIDIL 292 YT+ TGI+++ LNGD +V+IP D + + +I H+ +M +ID L Sbjct: 263 GYTIATGILNSNLNGDNIVSIPLDIDDPIELVYIQHEKTSLSKMGERFIDYL 314 >L0219 [K] COG0583 Transcriptional regulator Length = 301 Score = 180 bits (456), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 97/277 (35%), Positives = 165/277 (59%), Gaps = 2/277 (0%) Query: 1 LQQLRYAIEIANSGSMNEAAKRLFVSQPSLSNAIKELENELGITIFERNNRGISISAEGV 60 ++QLRY + IANSG+ EA+++LFVSQPS+S A+K+LE ELG IFER N G +++ EG Sbjct: 3 IKQLRYVVAIANSGTFREASEQLFVSQPSMSIAVKDLEQELGFQIFERTNTGATLTIEGE 62 Query: 61 EFLGYARQIIEQTEFMENRYTGKKRSPIYFSVSTQHYPFVVDAFVKLMKENKVTEYNFSL 120 F A+ ++ E E++Y+ K S FSV++QHY F+ V+ K+N + NF + Sbjct: 63 RFYEQAQTVLRNFETFESKYSKPKESDKTFSVASQHYDFLAPVAVEFAKKNPEIK-NFRI 121 Query: 121 RETQTYEIIEDVRTLRSDLGILYINEGNYKVMNKLFSDGNLKFTPLFNTNPHVYVRAGHM 180 E+ TY I+++V S+LG++Y+N+ N + ++ + L + +F + H+Y+R GH Sbjct: 122 FESTTYNILQEVAQGHSELGVVYLNKQNRSGILRMLNKLELDYEEIFLSQTHIYIRKGHP 181 Query: 181 LAGKELITLEDILPYPYITFEQGENNSLHFSEEMLSFTQIEKNIQVTDRATLTHLLLGSD 240 LA +E I+ +D+ P + F Q L++SE+++ + VTDRA+L +L +D Sbjct: 182 LAQRETISDDDLKPLDRVRFTQENEQFLYYSEDLIETFENTLIYNVTDRASLNGILERTD 241 Query: 241 SYTVGTGIMDAELNGDGLVTIPFDSPEMFTVGWIAHK 277 +Y G G +D E + + +P + ++ + H+ Sbjct: 242 AYATGLGFIDKE-SVHNVTVVPMEGDNGNSLILVKHR 277 >SP0927 [K] COG0583 Transcriptional regulator Length = 302 Score = 167 bits (424), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 91/259 (35%), Positives = 157/259 (60%), Gaps = 8/259 (3%) Query: 1 LQQLRYAIEIANSGSMNEAAKRLFVSQPSLSNAIKELENELGITIFERNNRGISISAEGV 60 +QQLRY + IANSG+ EAA++++VSQPSLS ++++LE ELG IF R + G ++ G+ Sbjct: 3 IQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELGFKIFRRTSSGTFLTRRGM 62 Query: 61 EFLGYARQIIEQTEFMENRYTGKKRSPIYFSVSTQHYPFV---VDAFVKLMKENKVTEYN 117 EF ++++++ + +N+Y + FSV++QHY F+ + AF + + K N Sbjct: 63 EFYEKSQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFLPPTITAFSERYPDYK----N 118 Query: 118 FSLRETQTYEIIEDVRTLRSDLGILYINEGNYKVMNKLFSDGNLKFTPLFNTNPHVYVRA 177 F + E+ T +I+++V S++GI+Y+N N K + + L+ L + H+Y+R Sbjct: 119 FRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKLGLEVIELIPFHTHIYLRE 178 Query: 178 GHMLAGKELITLEDILPYPYITFEQGENNSLHFSEEMLSFTQIEKNIQVTDRATLTHLLL 237 GH LA KE + +ED+ P + F Q ++ L++SE + + + VTDRATL +L Sbjct: 179 GHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDTSASSQMFNVTDRATLNGILE 238 Query: 238 GSDSYTVGTGIMDAE-LNG 255 +D+Y G+G +D++ +NG Sbjct: 239 RTDAYATGSGFLDSDSVNG 257 >SPy0824 [K] COG0583 Transcriptional regulator Length = 304 Score = 156 bits (394), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 92/297 (30%), Positives = 163/297 (54%), Gaps = 8/297 (2%) Query: 1 LQQLRYAIEIANSGSMNEAAKRLFVSQPSLSNAIKELENELGITIFERNNRGISISAEGV 60 +QQLRY + IAN+G+ EAA +LFVSQPSLS +IK+LE ELG IF R G ++++G+ Sbjct: 3 IQQLRYVVAIANNGTFREAASKLFVSQPSLSVSIKDLEAELGFQIFNRTTSGTVLTSQGL 62 Query: 61 EFLGYARQIIEQTEFMENRYTGKKRSPIYFSVSTQHYPF---VVDAFVKLMKENKVTEYN 117 F A ++++ + E ++ FS+++QHY F ++ AF + ++V Sbjct: 63 VFYEKALEVVKSFDSFEKTFSQADLDQNEFSIASQHYDFLPPLITAFSQQYDGHRV---- 118 Query: 118 FSLRETQTYEIIEDVRTLRSDLGILYINEGNYKVMNKLFSDGNLKFTPLFNTNPHVYVRA 177 F + E+ T +I+++V S++GI+Y+N N K + + L++ L H+Y+ Sbjct: 119 FRIFESTTIQILDEVAQGNSEIGIIYLNVDNQKGLFQRMDKLGLEYVSLIPFTTHIYLSK 178 Query: 178 GHMLAGKELITLEDILPYPYITFEQGENNSLHFSEEMLSFTQIEKNIQVTDRATLTHLLL 237 H LA +E + L DI P + F Q + L++SE + ++ + V+DRATL +L Sbjct: 179 THPLANREALYLNDIQGLPAVRFTQERDEYLYYSENFVDTSECPRIYNVSDRATLNGILE 238 Query: 238 GSDSYTVGTGIMDAELNGDGLVTIPFDSPEMFTVGWIAHKDRKPGEMMSAYIDILND 294 ++++ G+G +D + +G+ IP + ++ KD+ + ++ IL D Sbjct: 239 RTNAFATGSGFLDHR-SVNGIKVIPLADHIDNQMIYVKRKDKNLSVAGATFVTILKD 294 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,839,093 Number of extensions: 943563 Number of successful extensions: 3907 Number of sequences better than 1.0e-05: 1440 Number of HSP's gapped: 3888 Number of HSP's successfully gapped: 1440 Length of query: 308 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 202 Effective length of database: 26,153,027 Effective search space: 5282911454 Effective search space used: 5282911454 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)