BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0099.0007.p (371 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0838 [E] COG0620 Methionine synthase II (cobalamin-independent) 384 e-106 BS_yxjH [E] COG0620 Methionine synthase II (cobalamin-independent) 301 1e-81 BS_yxjG [E] COG0620 Methionine synthase II (cobalamin-independent) 291 1e-78 L124252 [E] COG0620 Methionine synthase II (cobalamin-independent) 279 6e-75 CPn0143 [E] COG0620 Methionine synthase II (cobalamin-independent) 276 3e-74 >lin0838 [E] COG0620 Methionine synthase II (cobalamin-independent) Length = 367 Score = 384 bits (986), Expect = e-106, Method: Compositional matrix adjust. Identities = 182/360 (50%), Positives = 238/360 (66%), Gaps = 3/360 (0%) Query: 14 PFRYDIVGSFLRPEALKAARSLHLQGEITDGQLHEIENAEISKLLEQEKALGLKAVTDGE 73 PF D VGS LR +A+K AR GEIT +L EIEN EI ++E++K +GLKA+TDGE Sbjct: 6 PFYADHVGSILRTKAIKEAREKFQSGEITTQELREIENEEIKYIVEKQKEVGLKAITDGE 65 Query: 74 FRRSWWHLDFFLGVAGTQKISLDQGRSAKEAVQRAESFRIVGKIAFGDHPMVSQFVELQQ 133 FRR+WWH DF + G + G + ++ S +I G I F HP + F+ L++ Sbjct: 66 FRRAWWHFDFLENLDGVEGYDAAGGIQFSKVQTKSHSVKITGPIDFTTHPFIEDFIFLKE 125 Query: 134 MAGDT-LAKMTIPSPALFHFVESYNGNEVYPDREALFRDIIQVYQDAIRAFYEAGCRYLQ 192 G+ +AK TIPSPA+ H+ D + D+ YQ AI+AFY+AGCRYLQ Sbjct: 126 AVGENHVAKQTIPSPAMLHYRGDMEYQPYIDDADKFVADLAAAYQKAIQAFYDAGCRYLQ 185 Query: 193 LDDTTWGTLCSGRHRAHLRSRGIDPDQLAQDYVRLINESIAGRPADMTIALHVCRGNFRS 252 LDDT+W LCS R +R RG DPD L + Y LINE+I +PADM I +H+CRGNFRS Sbjct: 186 LDDTSWSYLCSDEQREVVRERGFDPDTLQETYKTLINEAIKYKPADMVITMHICRGNFRS 245 Query: 253 TWFAAGGYEPVAEVLFGQAEVDAFFLEYDNERSGDFAPLRFIR--DQQVVLGLVTTKHGG 310 TW A GGY PVAE LFG+ +D FFLEYDNERSGDF+PL+++ D ++VLGL+T+K G Sbjct: 246 TWIAEGGYGPVAETLFGKLNIDGFFLEYDNERSGDFSPLKYVARPDLKIVLGLITSKTGD 305 Query: 311 LESKEQXXXXXXXXXXXVPIDQLCLSAQCGFASTEEGNMLTEEEQWDKLRLIIETADEVW 370 LE + VP++QLCLS QCGFASTEEGN+L+EEEQW+KLR +++ A++VW Sbjct: 306 LEDETAIKARIKEASEIVPLNQLCLSPQCGFASTEEGNILSEEEQWNKLRYVVKIAEDVW 365 >BS_yxjH [E] COG0620 Methionine synthase II (cobalamin-independent) Length = 340 Score = 301 bits (771), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 143/309 (46%), Positives = 202/309 (65%), Gaps = 3/309 (0%) Query: 9 KRNTPPFRYDIVGSFLRPEALKAARSLHLQGEITDGQLHEIENAEISKLLEQEKALGLKA 68 K+ PFR D VGS LR +K AR GE+T QL +IEN EI++++E++K +GL Sbjct: 14 KQRKAPFRADHVGSLLRSVPVKEARQKKAAGEMTAEQLRDIENQEITRIVEKQKEIGLDV 73 Query: 69 VTDGEFRRSWWHLDFFLGVAGTQKISLDQGRSAKEAVQRAESFRIVGKIAFGDHPMVSQF 128 VTDGEFRRSWWH DF G+ G + +G +A S ++ GK+ F HP + + Sbjct: 74 VTDGEFRRSWWHYDFLEGLDGVEPFIPAEGIQFHNTKTKARSIKVTGKLDFTSHPALGDY 133 Query: 129 VELQQMAGDTLAKMTIPSPALFHFVESYNGNEVYPDREALFRDIIQVYQDAIRAFYEAGC 188 L ++AG+ K+TIPSP + F E + +Y D+E F D+ Q Y+ AI+AFY+AGC Sbjct: 134 QFLHEIAGNATPKLTIPSPNMLFFGEKADKG-IYDDQEEYFHDMAQAYKKAIKAFYDAGC 192 Query: 189 RYLQLDDTTWGTLCSGRHRAHLRSRGIDPDQLAQDYVRLINESIAGRPADMTIALHVCRG 248 RYLQLDDT+W + R +R+ G DP+ L + + IN+++A RP D+TI +H+CRG Sbjct: 193 RYLQLDDTSWSLFFEDKGREVVRALGGDPETLPAIFAKTINDAVADRPDDLTITMHICRG 252 Query: 249 NFRSTWFAAGGYEPVAEVLFGQAEVDAFFLEYDNERSGDFAPLRFI--RDQQVVLGLVTT 306 NFRSTW A+GGY+ VAE + +D FLEYD++RSG+F PLRF+ +D Q+VLGL+T+ Sbjct: 253 NFRSTWAASGGYDAVAETILDGLNLDGLFLEYDDDRSGNFDPLRFVKRKDLQIVLGLITS 312 Query: 307 KHGGLESKE 315 K+G LE+ E Sbjct: 313 KYGELENPE 321 >BS_yxjG [E] COG0620 Methionine synthase II (cobalamin-independent) Length = 211 Score = 291 bits (746), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 0/309 (0%), Positives = 0/309 (0%), Gaps = 2/309 (0%) Query: 9 KRNTPPFRYDIVGSFLRPEALKAARSLHLQGEITDGQLHEIENAEISKLLEQEKALGLKA 68 + N P ++ S + S + ++ + E + Sbjct: 14 RENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPAAMLELGVEGVK 73 Query: 69 VTDGEFRRSWWHLDFFLGVAGTQKISLDQGRSAKEAVQRAESFRIVGKIAFGDHPMVSQF 128 + + + A A + Sbjct: 74 TYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRKTANVVLNTAFGWP 133 Query: 129 VELQQMAGDTLAKMTIPSPALFHFVESYNGNEVYPDREALFRDIIQVYQDAIRAFYEAGC 188 + T +P +V P + + Sbjct: 134 TIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLILHGRYTCIARKPR 193 Query: 189 RYLQLDDTTWGTLCSGRHRAHLRSRGIDPDQLAQDYVRLINESIAGRPADMTIALHVCRG 248 Sbjct: 194 253 Query: 249 NFRSTWFAAGGYEPVAEVLFGQAEVDAFFLEYDNERSGDFAPLRFIR--DQQVVLGLVTT 306 Sbjct: 254 313 Query: 307 KHGGLESKE 315 Sbjct: 314 322 >L124252 [E] COG0620 Methionine synthase II (cobalamin-independent) Length = 369 Score = 279 bits (713), Expect = 6e-75, Method: Compositional matrix adjust. Identities = 155/374 (41%), Positives = 219/374 (58%), Gaps = 31/374 (8%) Query: 15 FRYDIVGSFLRPEALKAARSLHLQGEITDGQLHEIENAEISKLLEQEKALGLKAVTDGEF 74 + +D VGSFLRP+ LK AR + +G++ +I+ +EI +L+++E +GLKAVTDGEF Sbjct: 8 YHFDHVGSFLRPQTLKEARLAYSEGQLPLSDFKKIQESEIKRLVKEEVNVGLKAVTDGEF 67 Query: 75 RRSWWHLDFFLGVAGTQKISLDQGRSAKEAVQRAESFRIVGKIAFG-DHPMVSQFVELQQ 133 RSWWHLDF G+ G + A R + + GK+AF +HP + F L+ Sbjct: 68 NRSWWHLDFLWGLNGVGTYEQEDSYKFHGAKTRTTNIELTGKVAFNPEHPFFADFNYLKS 127 Query: 134 MAGDTL-AKMTIPSPALFHFVESYNGN-----EVYPDREALFRDIIQVYQDAIRAFYEAG 187 + + + K+TIPSP+L + + +G E Y E D+ Q Y + + FYE G Sbjct: 128 LLPEGVEPKVTIPSPSL---IPNRDGRSDRWPEFYGSWEEFLDDLAQAYHETLLHFYELG 184 Query: 188 CRYLQLDDTTWGTLCSGRHRAHLRSRGI-DPD------QLAQDYVRLINESIAGRPADMT 240 RY+QLDDTTW A+L +R + DP+ ++ +D V L+N+ + P D+ Sbjct: 185 ARYIQLDDTTW---------AYLIARLLDDPENHEKYVKMCEDIVYLVNKLLKDLPEDLR 235 Query: 241 IALHVCRGNFRSTWFAAGGYEPVAEVLFGQAEVDAFFLEYDNERSGDFAPLRFIRDQQ-- 298 ++ H+CRGNF+ST+ G YEPVA+ L GQ D FFLEYD+ RSG FAPL I +Q+ Sbjct: 236 VSTHICRGNFKSTYLFEGSYEPVAKYL-GQLNYDTFFLEYDDARSGSFAPLAEIWNQRKD 294 Query: 299 --VVLGLVTTKHGGLESKEQXXXXXXXXXXXVPIDQLCLSAQCGFASTEEGNMLTEEEQW 356 +VLGL T+KH LE E +P++ L LS QCGFASTEEGN LTEEE+W Sbjct: 295 VLLVLGLFTSKHPELEKYEDIKARLDEALESIPLENLALSTQCGFASTEEGNDLTEEEEW 354 Query: 357 DKLRLIIETADEVW 370 KL I + D++W Sbjct: 355 AKLAHIKKIVDKIW 368 >CPn0143 [E] COG0620 Methionine synthase II (cobalamin-independent) Length = 374 Score = 276 bits (707), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 155/364 (42%), Positives = 213/364 (58%), Gaps = 13/364 (3%) Query: 17 YDIVGSFLRPEALKAARSLHLQGEITDGQLHEIENAEISKLLEQEKALGLKAVTDGEFRR 76 +D+VGSFLRPE LK R +G I+ QL +IE+ I L++++KA GL +TDGEFRR Sbjct: 13 FDVVGSFLRPEHLKKTRESLKEGSISLDQLMQIEDIAIQDLIKKQKAAGLSFITDGEFRR 72 Query: 77 SWWHLDFFLGVAGTQKISLDQGRSAKEAVQRAESFRIVGKIAFGDHPMVSQFVELQQMAG 136 + WH DF G G +G + + KI+ HP V F ++ + Sbjct: 73 ATWHYDFMWGFHGVGHHRATEGVFFDGERAMIDDTYLTDKISVSHHPFVDHFKFVKALED 132 Query: 137 D-TLAKMTIPSPALFHFVESYNGN-----EVYPDREALFRDIIQVYQDAIRAFYEAGCRY 190 + T AK T+P+PA F + N + YP + L DI+ Y+ IR Y+AGCRY Sbjct: 133 EFTTAKQTLPAPAQFLKQMIFPNNIEVTRKFYPTNQELIEDIVAGYRKVIRDLYDAGCRY 192 Query: 191 LQLDDTTWGTLCSGRHRAHLRSRGIDPD---QLAQDYVRLINESIAGRPADMTIALHVCR 247 LQLDD T G L R + GID L Q Y+ + N IA RP D+ + LHVCR Sbjct: 193 LQLDDCTRGGLVDPRVCSWY---GIDEKGLQDLIQQYLLINNLVIADRPDDLVVNLHVCR 249 Query: 248 GNFRSTWFAAGGYEPVAEVLFGQAEVDAFFLEYDNERSGDFAPLRFIR-DQQVVLGLVTT 306 GN+ S +FA+G Y+ +A+ LF Q VD ++LE+D+ERSGDF+PL FI ++ V LGLVT+ Sbjct: 250 GNYHSKFFASGSYDFIAKPLFEQTNVDGYYLEFDHERSGDFSPLTFISGEKTVCLGLVTS 309 Query: 307 KHGGLESKEQXXXXXXXXXXXVPIDQLCLSAQCGFASTEEGNMLTEEEQWDKLRLIIETA 366 K LE+K++ +P+++L LS QCGFAS E GN LTEEEQW K+ L+ E + Sbjct: 310 KTPTLENKDEVIARIHQAADYLPLERLSLSPQCGFASCEIGNKLTEEEQWAKVALVKEIS 369 Query: 367 DEVW 370 +EVW Sbjct: 370 EEVW 373 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.137 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,339,788 Number of extensions: 1093468 Number of successful extensions: 8921 Number of sequences better than 1.0e-05: 9 Number of HSP's gapped: 8900 Number of HSP's successfully gapped: 9 Length of query: 371 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 263 Effective length of database: 25,922,359 Effective search space: 6817580417 Effective search space used: 6817580417 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)