BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0099.0003.p (290 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2266 [R] COG0673 Predicted dehydrogenases and related proteins 411 e-115 SA0210 [R] COG0673 Predicted dehydrogenases and related proteins 392 e-109 BH2165 [R] COG0673 Predicted dehydrogenases and related proteins 192 6e-49 ZycjS [R] COG0673 Predicted dehydrogenases and related proteins 154 2e-37 ECs1894 [R] COG0673 Predicted dehydrogenases and related proteins 154 2e-37 >lin2266 [R] COG0673 Predicted dehydrogenases and related proteins Length = 358 Score = 411 bits (1057), Expect = e-115, Method: Compositional matrix adjust. Identities = 193/286 (67%), Positives = 226/286 (79%) Query: 1 VLTPNISHAEISIAALEAGKHVMCXXXXXXXXXXXXXXXXXXXRTGKKLTIGYNNRFRED 60 V TPNISHAEISIAA+EAGKHVMC RTGKKLTIGY NRFR+D Sbjct: 73 VCTPNISHAEISIAAMEAGKHVMCEKPMAKTTAEAKSMIEAANRTGKKLTIGYQNRFRKD 132 Query: 61 SLYLKQLCEAGELGSIYFAKAHAIRRRAVPTWGVFLDEEKQGGGPLIDIGTHALDLTLWM 120 S YL Q+CE GELG IY+AKA AIRRRAVPTWGVFLDEE QGGGPLIDIGTHALDLTLWM Sbjct: 133 SDYLHQVCENGELGDIYYAKAKAIRRRAVPTWGVFLDEEAQGGGPLIDIGTHALDLTLWM 192 Query: 121 MDNYQPKVVLGTTHHELSRRENAANAWGPWDPKQFSVEDSAFGMIVMENGATIMLESSWA 180 MDNY+PK V+G ++H+L+++ENAANAWG WDP +F+VEDSAFG I MENGATI+LE+SWA Sbjct: 193 MDNYKPKYVVGNSYHKLAKKENAANAWGSWDPSKFTVEDSAFGFITMENGATIVLEASWA 252 Query: 181 LNSLDVDEAKCSLSGTEAGADMKQGLRINGEKLGRLYTNEIELSAGGVAFYEGRQESAPD 240 LN+LDV EA+ SLSGTE GADM+ GLRINGE ++Y +I+L AGGV FY+G + Sbjct: 253 LNTLDVGEARTSLSGTEGGADMEDGLRINGEAHSQMYEKKIQLEAGGVDFYDGTGDDPAL 312 Query: 241 TEMRQWIEAIDQDKEPVVTPEQAYVVSRILEALYESSRTGKAIYLN 286 E QW+EAI D +PVV PEQA VV++ILEA+YESS+TG+ +Y N Sbjct: 313 IEAEQWLEAILNDTDPVVKPEQALVVTQILEAIYESSKTGQPVYFN 358 >SA0210 [R] COG0673 Predicted dehydrogenases and related proteins Length = 359 Score = 392 bits (1008), Expect = e-109, Method: Compositional matrix adjust. Identities = 181/285 (63%), Positives = 220/285 (77%) Query: 1 VLTPNISHAEISIAALEAGKHVMCXXXXXXXXXXXXXXXXXXXRTGKKLTIGYNNRFRED 60 V TPN SH EI++A L AGKHVMC TGKKLTIGY NRFR D Sbjct: 74 VCTPNDSHCEITVAGLHAGKHVMCEKPMAKTTAEAQKMIDTAKSTGKKLTIGYQNRFRPD 133 Query: 61 SLYLKQLCEAGELGSIYFAKAHAIRRRAVPTWGVFLDEEKQGGGPLIDIGTHALDLTLWM 120 S +L Q + G+LG IYF KAHAIRRRAVPTWGVFL+EE QGGGPLIDIGTHALDLTLWM Sbjct: 134 SQFLHQAAQRGDLGDIYFGKAHAIRRRAVPTWGVFLNEEAQGGGPLIDIGTHALDLTLWM 193 Query: 121 MDNYQPKVVLGTTHHELSRRENAANAWGPWDPKQFSVEDSAFGMIVMENGATIMLESSWA 180 MDNY+P+ V+G+T H+L+++ +AANAWG W+P +F+VEDSAFG I M+NGATI+LES+WA Sbjct: 194 MDNYEPESVMGSTFHKLNKQHDAANAWGSWNPDEFTVEDSAFGFIKMKNGATIILESAWA 253 Query: 181 LNSLDVDEAKCSLSGTEAGADMKQGLRINGEKLGRLYTNEIELSAGGVAFYEGRQESAPD 240 +NSL+VDEAKCSLSGT+AGADMK GLRI+GE +G LYT +EL GV FYEG + + Sbjct: 254 INSLEVDEAKCSLSGTKAGADMKDGLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAE 313 Query: 241 TEMRQWIEAIDQDKEPVVTPEQAYVVSRILEALYESSRTGKAIYL 285 E + WI+A+ D EPVV PEQA VV++ILEA+Y+S+++GKAIY Sbjct: 314 EEAKAWIDAVVNDTEPVVKPEQAMVVTKILEAIYQSAKSGKAIYF 358 >BH2165 [R] COG0673 Predicted dehydrogenases and related proteins Length = 348 Score = 192 bits (488), Expect = 6e-49, Method: Compositional matrix adjust. Identities = 103/283 (36%), Positives = 158/283 (55%), Gaps = 11/283 (3%) Query: 1 VLTPNISHAEISIAALEAGKHVMCXXXXXXXXXXXXXXXXXXXRTGKKLTIGYNNRFRED 60 + TPN H E++I AL AG HV+C TG LT G++ R + Sbjct: 75 ICTPNAFHRELTIEALHAGCHVLCEKPPAITVEDAKQMEAAAKETGNLLTYGFHYRHSPE 134 Query: 61 SLYLKQLCEAGELGSIYFAKAHAIRRRAVPTWGVFLDEEKQGGGPLIDIGTHALDLTLWM 120 LK+ + GELG IY + AIRRR +P WGVF ++E QGGGPLIDIG H LD L++ Sbjct: 135 VETLKRFVDEGELGEIYHVRVQAIRRRGIPGWGVFTNKELQGGGPLIDIGVHMLDTALYV 194 Query: 121 MDNYQPKVVLGTTHHELSRRENAANAWGPWDPKQFSVEDSAFGMIVMENGATIMLESSWA 180 M +P +LG TH ++ +E G W+ +QFS+ED A GMI +NGA++ +E+++A Sbjct: 195 MGFPEPATILGQTHRKIGTKE-GVGLLGEWNWQQFSIEDMAVGMITFKNGASLTIETAFA 253 Query: 181 LNSLDVDEAKCSLSGTEAGADMKQGLRINGEKLGRLYTNEIELSAGGVAFYEGRQESAPD 240 N + D S G + GAD+ L I EK L ++++ + Y + Sbjct: 254 ANVKEQDHMNVSFFGDKGGADLFP-LSIYQEKHRTL----LDITPVYLPTYNHHER---- 304 Query: 241 TEMRQWIEAIDQDKEPVVTPEQAYVVSRILEALYESSRTGKAI 283 E++ +++ + ++P+ TPEQ V+ R+++ LY+S+ +GKAI Sbjct: 305 -EIKHFVDCCFRLEQPLATPEQGIVIQRLIQGLYQSAESGKAI 346 >ZycjS [R] COG0673 Predicted dehydrogenases and related proteins Length = 351 Score = 154 bits (388), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 95/289 (32%), Positives = 139/289 (48%), Gaps = 19/289 (6%) Query: 1 VLTPNISHAEISIAALEAGKHVMCXXXXXXXXXXXXXXXXXXXRTGKKLTIGYNNRFRED 60 V +PN H E ++ ALEAG HVMC + GK L +++RF D Sbjct: 78 VCSPNRFHYEHTLMALEAGCHVMCEKPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALD 137 Query: 61 SLYLKQLCEAGELGSIYFAKAHAIRRRAVPTWGVFLDEEKQGGGPLIDIGTHALDLTLWM 120 + L++ G LG IY A A+RR VP WGVF ++E QGGGPLIDIG H LD +++ Sbjct: 138 TQQLREQVTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYV 197 Query: 121 MDNYQPKVVLGTTHHELSRRENAANAWGPWDPKQFSVEDSAFGMIVMENGATIMLESSWA 180 + K V + ++ +++ +G WDP +SVEDS FG I NG + LE+S+A Sbjct: 198 LGFPAVKSVNAHSFQKIGTQKSCGQ-FGEWDPATYSVEDSLFGTIEFHNGGILWLETSFA 256 Query: 181 LNSLDVDEAKCSLSGTEAGADMKQGLRINGEKLGRLYTNEIELSAGGVAFYEGRQESAPD 240 LN + S G +AGA L+ I G ++E A D Sbjct: 257 LNIREQSIMNVSFCGDKAGA--------------TLFPAHIYTDNNGELMTLMQREIADD 302 Query: 241 TE----MRQWIEAIDQDKEPVVTPEQAYVVSRILEALYESSRTGKAIYL 285 M +I + + EQ Y++ +++ ALY+S+ TG + L Sbjct: 303 NRHLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL 351 >ECs1894 [R] COG0673 Predicted dehydrogenases and related proteins Length = 351 Score = 154 bits (388), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 95/289 (32%), Positives = 139/289 (48%), Gaps = 19/289 (6%) Query: 1 VLTPNISHAEISIAALEAGKHVMCXXXXXXXXXXXXXXXXXXXRTGKKLTIGYNNRFRED 60 V +PN H E ++ ALEAG HVMC + GK L +++RF D Sbjct: 78 VCSPNRFHYEHTLMALEAGCHVMCEKPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALD 137 Query: 61 SLYLKQLCEAGELGSIYFAKAHAIRRRAVPTWGVFLDEEKQGGGPLIDIGTHALDLTLWM 120 + L++ G LG IY A A+RR VP WGVF ++E QGGGPLIDIG H LD +++ Sbjct: 138 TQQLREQVTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYV 197 Query: 121 MDNYQPKVVLGTTHHELSRRENAANAWGPWDPKQFSVEDSAFGMIVMENGATIMLESSWA 180 + K V + ++ +++ +G WDP +SVEDS FG I NG + LE+S+A Sbjct: 198 LGYPAVKSVNAHSFQKIGTQKSCGQ-FGEWDPATYSVEDSLFGTIEFHNGGILWLETSFA 256 Query: 181 LNSLDVDEAKCSLSGTEAGADMKQGLRINGEKLGRLYTNEIELSAGGVAFYEGRQESAPD 240 LN + S G +AGA L+ I G ++E A D Sbjct: 257 LNIREQSIMNVSFCGDKAGA--------------TLFPAHIYTDNNGELMTLMQREMADD 302 Query: 241 TE----MRQWIEAIDQDKEPVVTPEQAYVVSRILEALYESSRTGKAIYL 285 M +I + + EQ Y++ +++ ALY+S+ TG + L Sbjct: 303 NRHLGSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL 351 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.132 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,746,153 Number of extensions: 834009 Number of successful extensions: 7667 Number of sequences better than 1.0e-05: 37 Number of HSP's gapped: 7652 Number of HSP's successfully gapped: 37 Length of query: 290 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 185 Effective length of database: 26,268,361 Effective search space: 4859646785 Effective search space used: 4859646785 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)