BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0094.0008.p (444 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_licH [G] COG1486 Alpha-galactosidases/6-phospho-beta-glucosid... 506 e-143 lin0526 [G] COG1486 Alpha-galactosidases/6-phospho-beta-glucosid... 505 e-143 BH0183 [G] COG1486 Alpha-galactosidases/6-phospho-beta-glucosida... 498 e-140 lin0540 [G] COG1486 Alpha-galactosidases/6-phospho-beta-glucosid... 459 e-129 BH0912 [G] COG1486 Alpha-galactosidases/6-phospho-beta-glucosida... 449 e-126 >BS_licH [G] COG1486 Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases Length = 442 Score = 506 bits (1303), Expect = e-143, Method: Compositional matrix adjust. Identities = 245/438 (55%), Positives = 327/438 (74%), Gaps = 5/438 (1%) Query: 4 NQGLKIAVIGGGSSYTPELVEGFILHHKVLPVRELWLVDIEPGLRKLNIVGSLAKRMVEK 63 +GLKI IGGGSSYTPELVEGFI + LPVRELWLVDI G KLNIVG+LAKRMVEK Sbjct: 2 TKGLKIVTIGGGSSYTPELVEGFIKRYDELPVRELWLVDIPEGEEKLNIVGTLAKRMVEK 61 Query: 64 SGLPIEVHLTTDRRKAIEGADFVSTQIRVGMLDARGRDESIPLKYGVIGQETTGPGGMLK 123 +G+PI++HLT DRRKA++ ADFV+TQ RVG+L AR +DE IPLKYGVIGQET GPGG+ K Sbjct: 62 AGVPIDIHLTLDRRKALKDADFVTTQFRVGLLQARAKDERIPLKYGVIGQETNGPGGLFK 121 Query: 124 ALRTIPVILDICRDIEELAPNAWLLNFTNPAGMVTEAVLRYSNVKS-IGLCNAPIGLIKQ 182 LRTIPVIL+I +DIEEL PNAWL+NFTNPAGMVTEA+LRYSN+K +GLCN PIG+ Sbjct: 122 GLRTIPVILEIAKDIEELCPNAWLVNFTNPAGMVTEALLRYSNLKKVVGLCNVPIGIKMG 181 Query: 183 VSAKYNAAPDRIYAEFVGLNHLHWITRIDVEG---EDKLDEMLAD-TAGYSAKNVPAREW 238 V+ + DR+ +F GLNH+ + + ++G ++++ E + D + KN+ EW Sbjct: 182 VAKALDVDVDRVEVQFAGLNHMVFGLDVFLDGVSVKEQVIEAMGDPKNAMTMKNISGAEW 241 Query: 239 NPEFLQSLRALPSYYLKYFYMTDAMLAEQLESFKQGGNRAEVVKRVEEELFEIYGNPELN 298 P+FL++L +P Y +Y++ T ML +LE+ + G RAEVV++VE+ELFE+Y +P L Sbjct: 242 EPDFLKALNVIPCGYHRYYFKTKEMLEHELEASQTEGTRAEVVQKVEKELFELYKDPNLA 301 Query: 299 EKPKQLEQRGGAFYSEAAVNLMRSLHNGTNDIQTLNVANRGILDFLPEDASIEVNCVVTK 358 KP QLE+RGGA+YS+AA NL+ S++N +DIQ +N N G + +P+D+++EVNCV+TK Sbjct: 302 IKPPQLEKRGGAYYSDAACNLISSIYNDKHDIQPVNTINNGAIASIPDDSAVEVNCVMTK 361 Query: 359 TGPLPLPLTKIPPMAKGLIHAVKTYEQLAIDAAVTGDRSLAIQALAHHPLVPSVEVAIAM 418 TGP P+ + +P +GL+ +K++E++A +AAVTGD A+ A+ +PLVPS VA + Sbjct: 362 TGPKPIAVGDLPVSVRGLVQQIKSFERVAAEAAVTGDYQTALLAMTINPLVPSDTVAKQI 421 Query: 419 LDEMLEANKEYLPSFFAK 436 LDEMLEA+K YLP FF K Sbjct: 422 LDEMLEAHKAYLPQFFNK 439 >lin0526 [G] COG1486 Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases Length = 438 Score = 505 bits (1301), Expect = e-143, Method: Compositional matrix adjust. Identities = 249/436 (57%), Positives = 316/436 (72%), Gaps = 7/436 (1%) Query: 5 QGLKIAVIGGGSSYTPELVEGFILHHKVLPVRELWLVDIEPGLRKLNIVGSLAKRMVEKS 64 +G+KIA IGGGSSYTPEL+EGFI LPVRELWLVD+E G KL IVG+LAKRMVEK+ Sbjct: 3 KGIKIATIGGGSSYTPELIEGFIKRQDELPVRELWLVDVEAGREKLEIVGNLAKRMVEKA 62 Query: 65 GLPIEVHLTTDRRKAIEGADFVSTQIRVGMLDARGRDESIPLKYGVIGQETTGPGGMLKA 124 G+ +EVHLT DR +A++ ADFV+TQ+RVG+LDAR +DE IP YGV+GQET GPGGM K Sbjct: 63 GVDMEVHLTLDREEALKDADFVTTQLRVGLLDARVKDERIPNSYGVVGQETNGPGGMFKG 122 Query: 125 LRTIPVILDICRDIEELAPNAWLLNFTNPAGMVTEAVLRYSNVKS-IGLCNAPIGLIKQV 183 LRTIPVILDIC+D+E L P+AWL+NF NPAGMVTEAVLRYSN K +GLCN PIG+ + + Sbjct: 123 LRTIPVILDICKDMERLCPDAWLINFANPAGMVTEAVLRYSNQKKVVGLCNGPIGIERNI 182 Query: 184 SAKYNAAPDRIYAEFVGLNHLHWITRIDVEGEDKLDE----MLADTAGYSAKNVPAREWN 239 + IY EFVGLNH+ + + G+D + M D AG S KN+ A W+ Sbjct: 183 AETLGVDVSEIYVEFVGLNHMVFAKTVYHNGKDVTKDVVFKMTEDEAGSSLKNINATGWD 242 Query: 240 PEFLQSLRALPSYYLKYFYMTDAMLAEQLESFKQGGNRAEVVKRVEEELFEIYGNPELNE 299 FL++L +P YL+Y++ T L +Q ++ + G RAEVVK+VE ELFE+Y EL E Sbjct: 243 KTFLRTLNMIPIDYLRYYWQTKQQLEDQARAYAEHGTRAEVVKKVEAELFELYKQEELAE 302 Query: 300 KPKQLEQRGGAFYSEAAVNLMRSLHNGTNDIQTLNVANRG-ILDFLPEDASIEVNCVVTK 358 KPKQLEQRGGA+YSEAA NL+ S++N DIQ +N N G ILD P D+++E NCV+T+ Sbjct: 303 KPKQLEQRGGAYYSEAACNLINSIYNDKRDIQIVNTRNNGAILDIDP-DSAVETNCVITR 361 Query: 359 TGPLPLPLTKIPPMAKGLIHAVKTYEQLAIDAAVTGDRSLAIQALAHHPLVPSVEVAIAM 418 GP+PL ++P G+I +KT+E+L +AAVTGD A+ A+ +PL PS VA M Sbjct: 362 QGPIPLASGRLPIAINGIIQEIKTFERLTAEAAVTGDYDKALLAMTINPLTPSESVAREM 421 Query: 419 LDEMLEANKEYLPSFF 434 LDEMLEA+KEYLP+FF Sbjct: 422 LDEMLEAHKEYLPNFF 437 >BH0183 [G] COG1486 Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases Length = 442 Score = 498 bits (1281), Expect = e-140, Method: Compositional matrix adjust. Identities = 239/436 (54%), Positives = 325/436 (74%), Gaps = 5/436 (1%) Query: 4 NQGLKIAVIGGGSSYTPELVEGFILHHKVLPVRELWLVDIEPGLRKLNIVGSLAKRMVEK 63 +G+KIA IGGGSSYTPELVEGFI + LPVRELWLVDIE G K+ IVG+LAKRMV+K Sbjct: 2 TEGIKIATIGGGSSYTPELVEGFIKRYDELPVRELWLVDIEEGKEKMEIVGNLAKRMVQK 61 Query: 64 SGLPIEVHLTTDRRKAIEGADFVSTQIRVGMLDARGRDESIPLKYGVIGQETTGPGGMLK 123 +GLPI++HLT DRR+A++GADFV+TQ RVG+LDAR +DE IPLKYGV+GQET GPGG+ K Sbjct: 62 AGLPIDIHLTLDRREALKGADFVTTQFRVGLLDARAKDERIPLKYGVLGQETNGPGGLFK 121 Query: 124 ALRTIPVILDICRDIEELAPNAWLLNFTNPAGMVTEAVLRYSNVKS-IGLCNAPIGLIKQ 182 LRTIPVILDICRD+EEL P+AWL+NFTNPAGMVTEAVLRY+N+K +GLCN PIG+ Sbjct: 122 GLRTIPVILDICRDMEELCPDAWLINFTNPAGMVTEAVLRYANIKKVVGLCNVPIGIEMG 181 Query: 183 VSAKYNAAPDRIYAEFVGLNHLHWITRIDVEG---EDKLDEMLADTAGYS-AKNVPAREW 238 V+ + RI +F GLNH+ + + ++G +DK+ EM+ D S KN+ W Sbjct: 182 VAKLLDVDHSRIRIDFAGLNHMVYGLDVYLDGVSVKDKVIEMITDPNNSSFVKNIEGLGW 241 Query: 239 NPEFLQSLRALPSYYLKYFYMTDAMLAEQLESFKQGGNRAEVVKRVEEELFEIYGNPELN 298 P F+++L L Y Y+Y + M+ + L+ K+ G RAEVV+R+E+ELFE+Y +P L+ Sbjct: 242 EPSFVKALGVLTCPYHMYYYKSREMVEKDLKKAKEEGTRAEVVQRLEKELFELYKDPNLD 301 Query: 299 EKPKQLEQRGGAFYSEAAVNLMRSLHNGTNDIQTLNVANRGILDFLPEDASIEVNCVVTK 358 KP QLE+RGGA+YS+AAV L+ S++ DIQ +N NRG + +P+D+++EV+CV+TK Sbjct: 302 IKPPQLEERGGAYYSDAAVRLIHSIYTDRRDIQPVNTMNRGAIASIPDDSAVEVSCVITK 361 Query: 359 TGPLPLPLTKIPPMAKGLIHAVKTYEQLAIDAAVTGDRSLAIQALAHHPLVPSVEVAIAM 418 GP P+ + +P +GL+ +K++E++A +AAVTGD L + A+ +PL PS +A + Sbjct: 362 DGPKPIAMGDLPVPVRGLVQQIKSFERVAAEAAVTGDYDLTVLAMTINPLSPSDTIAKQI 421 Query: 419 LDEMLEANKEYLPSFF 434 +D+MLEA+KE+LP FF Sbjct: 422 VDDMLEAHKEHLPQFF 437 >lin0540 [G] COG1486 Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases Length = 440 Score = 459 bits (1180), Expect = e-129, Method: Compositional matrix adjust. Identities = 227/436 (52%), Positives = 304/436 (69%), Gaps = 7/436 (1%) Query: 5 QGLKIAVIGGGSSYTPELVEGFILHHKVLPVRELWLVDIEPGLRKLNIVGSLAKRMVEKS 64 +G+KI IGGGSSYTPELVEGFI + LP+RELWLVDIE G KL IVG++AKRMV+ + Sbjct: 3 KGVKIVTIGGGSSYTPELVEGFIKRYHELPIRELWLVDIEAGREKLEIVGNMAKRMVKAA 62 Query: 65 GLPIEVHLTTDRRKAIEGADFVSTQIRVGMLDARGRDESIPLKYGVIGQETTGPGGMLKA 124 + EVHLT DRR+A++ ADFV+TQ RVG+LDAR +DE IPL +G+IGQET G GGM KA Sbjct: 63 NIDCEVHLTLDRREALKDADFVTTQFRVGLLDARIKDERIPLSHGIIGQETNGAGGMFKA 122 Query: 125 LRTIPVILDICRDIEELAPNAWLLNFTNPAGMVTEAVLRYSNV-KSIGLCNAPIGLIKQV 183 RTIPVIL I D+ EL P+AWL+NFTNPAGMVTEAVLRY N K IGLCN PIG +K Sbjct: 123 FRTIPVILGIVEDMRELCPDAWLINFTNPAGMVTEAVLRYGNWDKVIGLCNVPIGAVKSA 182 Query: 184 SAKYNAAPDRIYAEFVGLNHLHWITRIDVEG----EDKLDEMLADTA--GYSAKNVPARE 237 S + ++ +F G+NHLHW D +G E +D + A A G +N+ Sbjct: 183 SDVLEKPEEDLFFKFAGINHLHWHRVFDKDGTELTEKVIDGLYAPDANPGKVVENIKNMR 242 Query: 238 WNPEFLQSLRALPSYYLKYFYMTDAMLAEQLESFKQGGNRAEVVKRVEEELFEIYGNPEL 297 + E ++ L+ LP Y +Y+YMTDAML E+L SFK G R EVVK++E+ LFE+Y +P L Sbjct: 243 FLYEQVKHLKMLPCPYHRYYYMTDAMLEEELASFKNEGTRGEVVKKLEDSLFELYKDPNL 302 Query: 298 NEKPKQLEQRGGAFYSEAAVNLMRSLHNGTNDIQTLNVANRGILDFLPEDASIEVNCVVT 357 + KP++L +RGGA YS+AA ++ S++N + ++ N G +D +P D+++E+ V+ Sbjct: 303 DYKPEELSKRGGAHYSDAACEIINSIYNNKGTVMVVSTRNNGAIDDVPYDSAVEITSVIR 362 Query: 358 KTGPLPLPLTKIPPMAKGLIHAVKTYEQLAIDAAVTGDRSLAIQALAHHPLVPSVEVAIA 417 G P+ K PP +GL+ +K+ E+L I+AAVTGD + A+QA +PLVPS ++A Sbjct: 363 AHGAEPINFGKFPPAQRGLLQVMKSMEELTIEAAVTGDYATALQAFTSNPLVPSGDLAKT 422 Query: 418 MLDEMLEANKEYLPSF 433 +LDEMLEA+KE+LP F Sbjct: 423 ILDEMLEAHKEFLPQF 438 >BH0912 [G] COG1486 Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases Length = 441 Score = 449 bits (1155), Expect = e-126, Method: Compositional matrix adjust. Identities = 218/435 (50%), Positives = 309/435 (71%), Gaps = 5/435 (1%) Query: 7 LKIAVIGGGSSYTPELVEGFILHHKVLPVRELWLVDIEPGLRKLNIVGSLAKRMVEKSGL 66 LK+ VIGGGSSYTPE++EG I + +PVRE+ LVDIE G +K+ I+ +L++RM+E SG Sbjct: 3 LKVVVIGGGSSYTPEIIEGLITRYDQIPVREIVLVDIEAGAKKVAIIEALSRRMIENSGY 62 Query: 67 PIEVHLTTDRRKAIEGADFVSTQIRVGMLDARGRDESIPLKYGVIGQETTGPGGMLKALR 126 PI VH + D R ++EGA+FV+TQIRVG L+AR +DE IPLK+G+IGQET G GG+ KA R Sbjct: 63 PIHVHSSFDLRASLEGANFVTTQIRVGGLEAREKDERIPLKHGLIGQETNGAGGVFKAFR 122 Query: 127 TIPVILDICRDIEELAPNAWLLNFTNPAGMVTEAVLRYS-NVKSIGLCNAPIGLIKQVSA 185 TIPV+L+I + I ++ P AW++NFTNPAG+VTEA+L +S + K +G+CN P + ++ Sbjct: 123 TIPVLLEIAQAIHDICPQAWMINFTNPAGIVTEALLHHSPHKKVVGVCNIPFNMRTGIAE 182 Query: 186 KYNAAPDRIYAEFVGLNHLHWITRIDVEGEDKLDEMLA----DTAGYSAKNVPAREWNPE 241 ++ +++ EF+G+NH + R+ + GED+ E LA D YS N+ + W+ Sbjct: 183 MFSCPVEQVEIEFIGMNHFVFGKRVFIHGEDRTTEALAKLTEDKVDYSPANIVSLGWSKA 242 Query: 242 FLQSLRALPSYYLKYFYMTDAMLAEQLESFKQGGNRAEVVKRVEEELFEIYGNPELNEKP 301 F+ +L+ LP+ Y Y++ T+ +L + L++F+ G RAE+VK+VE+ LF Y NP L+EKP Sbjct: 243 FVTALQLLPNPYHLYYFQTEEVLKKDLDAFRNNGTRAEIVKQVEDSLFLTYQNPSLSEKP 302 Query: 302 KQLEQRGGAFYSEAAVNLMRSLHNGTNDIQTLNVANRGILDFLPEDASIEVNCVVTKTGP 361 K+LEQRGGA+YSEAA NLM SL+ DIQT+N N G + LP+DA +E+N V+TK GP Sbjct: 303 KELEQRGGAYYSEAACNLMESLYTNKQDIQTVNTLNNGSIRDLPDDAVVEINAVITKDGP 362 Query: 362 LPLPLTKIPPMAKGLIHAVKTYEQLAIDAAVTGDRSLAIQALAHHPLVPSVEVAIAMLDE 421 PL + KIPP KGLI +KT+EQL I AA++GD A AL +PLV + A +LDE Sbjct: 363 RPLAIGKIPPHIKGLIGHMKTFEQLVIKAALSGDYYDAYNALIMNPLVADEKKAKIVLDE 422 Query: 422 MLEANKEYLPSFFAK 436 +L+A+KE+LP F+ K Sbjct: 423 LLDAHKEHLPQFYRK 437 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 34,190,934 Number of extensions: 1466954 Number of successful extensions: 3995 Number of sequences better than 1.0e-05: 39 Number of HSP's gapped: 3873 Number of HSP's successfully gapped: 42 Length of query: 444 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 334 Effective length of database: 25,691,691 Effective search space: 8581024794 Effective search space used: 8581024794 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)