BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0094.0005.p (308 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGl249 [R] COG3608 Predicted deacylase 177 2e-44 AGl2465 [R] COG0705 Uncharacterized membrane protein (homolog of... 177 2e-44 AGl2460 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase 177 2e-44 AGl2475 [J] COG0008 Glutamyl- and glutaminyl-tRNA synthetases 177 2e-44 AGl2433 [I] COG0204 1-acyl-sn-glycerol-3-phosphate acyltransferase 177 2e-44 >AGl249 [R] COG3608 Predicted deacylase Length = 320 Score = 177 bits (448), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 104/276 (37%), Positives = 154/276 (55%), Gaps = 1/276 (0%) Query: 18 INNYVQSDGTFSQPIPGLHIRRYSRTGVDWEKAFYLPSVLIVAQGVKTVRLEKETFQIGK 77 I + DGTF +PGL + R S T + Y P++ +VAQG K L + +F Sbjct: 26 IARHAPHDGTFETQLPGLKLIRCSETTLPM-PVVYEPTLCLVAQGRKQAVLGQTSFVYDP 84 Query: 78 SRMFMIPVALPVSLRTIQANAEQPFIGIGLTLDPEKIAEFVPKVYPRGLPSVQTRCAGYT 137 S + V LPV I+A+ ++P++ + L LD ++A+ + + Sbjct: 85 SSYLVASVDLPVMGSVIEASKDKPYLSMQLDLDTVELADLAIRYPSEQHAPINGPIGLLL 144 Query: 138 AEADTGIIHAVTRLIECLHHPSDAELLAPIVKDEILMRLLLSPIGIHIAEMGFADTSVRQ 197 + D GII A +RL+ L P D + L+P+ K EIL RL+ G + ++ A V Q Sbjct: 145 NKVDEGIIDAASRLLALLDTPRDIDALSPLAKREILYRLITGVGGAALRQIVQAGAHVNQ 204 Query: 198 VAKAIEWLRSNFDQPMKVSELASLSHMSVSVFHKHFKAVTSISPLKYQKELRLQEARNLM 257 +A++I W+R +F + V A ++ MS S FH HFKAVT++SPL+++ +LRLQEAR LM Sbjct: 205 IARSIVWIRKHFRETCSVEAGAEVAGMSRSTFHAHFKAVTNMSPLEFRTQLRLQEARRLM 264 Query: 258 LSKQMDAITASQLVGYASASQFSRDYRSYFGNPPRR 293 LS+ +DA TA VGYAS SQFSR+Y FG P + Sbjct: 265 LSEGLDAATAGFQVGYASPSQFSREYGRVFGMSPAK 300 >AGl2465 [R] COG0705 Uncharacterized membrane protein (homolog of Drosophila rhomboid) Length = 320 Score = 177 bits (448), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 104/276 (37%), Positives = 154/276 (55%), Gaps = 1/276 (0%) Query: 18 INNYVQSDGTFSQPIPGLHIRRYSRTGVDWEKAFYLPSVLIVAQGVKTVRLEKETFQIGK 77 I + DGTF +PGL + R S T + Y P++ +VAQG K L + +F Sbjct: 26 IARHAPHDGTFETQLPGLKLIRCSETTLPM-PVVYEPTLCLVAQGRKQAVLGQTSFVYDP 84 Query: 78 SRMFMIPVALPVSLRTIQANAEQPFIGIGLTLDPEKIAEFVPKVYPRGLPSVQTRCAGYT 137 S + V LPV I+A+ ++P++ + L LD ++A+ + + Sbjct: 85 SSYLVASVDLPVMGSVIEASKDKPYLSMQLDLDTVELADLAIRYPSEQHAPINGPIGLLL 144 Query: 138 AEADTGIIHAVTRLIECLHHPSDAELLAPIVKDEILMRLLLSPIGIHIAEMGFADTSVRQ 197 + D GII A +RL+ L P D + L+P+ K EIL RL+ G + ++ A V Q Sbjct: 145 NKVDEGIIDAASRLLALLDTPRDIDALSPLAKREILYRLITGVGGAALRQIVQAGAHVNQ 204 Query: 198 VAKAIEWLRSNFDQPMKVSELASLSHMSVSVFHKHFKAVTSISPLKYQKELRLQEARNLM 257 +A++I W+R +F + V A ++ MS S FH HFKAVT++SPL+++ +LRLQEAR LM Sbjct: 205 IARSIVWIRKHFRETCSVEAGAEVAGMSRSTFHAHFKAVTNMSPLEFRTQLRLQEARRLM 264 Query: 258 LSKQMDAITASQLVGYASASQFSRDYRSYFGNPPRR 293 LS+ +DA TA VGYAS SQFSR+Y FG P + Sbjct: 265 LSEGLDAATAGFQVGYASPSQFSREYGRVFGMSPAK 300 >AGl2460 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase Length = 320 Score = 177 bits (448), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 104/276 (37%), Positives = 154/276 (55%), Gaps = 1/276 (0%) Query: 18 INNYVQSDGTFSQPIPGLHIRRYSRTGVDWEKAFYLPSVLIVAQGVKTVRLEKETFQIGK 77 I + DGTF +PGL + R S T + Y P++ +VAQG K L + +F Sbjct: 26 IARHAPHDGTFETQLPGLKLIRCSETTLPM-PVVYEPTLCLVAQGRKQAVLGQTSFVYDP 84 Query: 78 SRMFMIPVALPVSLRTIQANAEQPFIGIGLTLDPEKIAEFVPKVYPRGLPSVQTRCAGYT 137 S + V LPV I+A+ ++P++ + L LD ++A+ + + Sbjct: 85 SSYLVASVDLPVMGSVIEASKDKPYLSMQLDLDTVELADLAIRYPSEQHAPINGPIGLLL 144 Query: 138 AEADTGIIHAVTRLIECLHHPSDAELLAPIVKDEILMRLLLSPIGIHIAEMGFADTSVRQ 197 + D GII A +RL+ L P D + L+P+ K EIL RL+ G + ++ A V Q Sbjct: 145 NKVDEGIIDAASRLLALLDTPRDIDALSPLAKREILYRLITGVGGAALRQIVQAGAHVNQ 204 Query: 198 VAKAIEWLRSNFDQPMKVSELASLSHMSVSVFHKHFKAVTSISPLKYQKELRLQEARNLM 257 +A++I W+R +F + V A ++ MS S FH HFKAVT++SPL+++ +LRLQEAR LM Sbjct: 205 IARSIVWIRKHFRETCSVEAGAEVAGMSRSTFHAHFKAVTNMSPLEFRTQLRLQEARRLM 264 Query: 258 LSKQMDAITASQLVGYASASQFSRDYRSYFGNPPRR 293 LS+ +DA TA VGYAS SQFSR+Y FG P + Sbjct: 265 LSEGLDAATAGFQVGYASPSQFSREYGRVFGMSPAK 300 >AGl2475 [J] COG0008 Glutamyl- and glutaminyl-tRNA synthetases Length = 252 Score = 177 bits (448), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 0/276 (0%), Positives = 0/276 (0%), Gaps = 1/276 (0%) Query: 18 INNYVQSDGTFSQPIPGLHIRRYSRTGVDWEKAFYLPSVLIVAQGVKTVRLEKETFQIGK 77 + + + L F Sbjct: 26 RVPLENVEYVADLGCGPGNSTALLQQRWPA-ARITGIDSSPAMIAEARSALPDCQFVEAD 84 Query: 78 SRMFMIPVALPVSLRTIQANAEQPFIGIGLTLDPEKIAEFVPKVYPRGLPSVQTRCAGYT 137 R + AL + + L + V V T Sbjct: 85 IRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMRE 144 Query: 138 AEADTGIIHAVTRLIECLHHPSDAELLAPIVKDEILMRLLLSPIGIHIAEMGFADTSVRQ 197 + + +H D A D T +R Sbjct: 145 VAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRP 204 Query: 198 VAKAIEWLRSNFDQPMKVSELASLSHMSVSVFHKHFKAVTSISPLKYQKELRLQEARNLM 257 Sbjct: 205 264 Query: 258 LSKQMDAITASQLVGYASASQFSRDYRSYFGNPPRR 293 Sbjct: 265 300 >AGl2433 [I] COG0204 1-acyl-sn-glycerol-3-phosphate acyltransferase Length = 320 Score = 177 bits (448), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 104/276 (37%), Positives = 154/276 (55%), Gaps = 1/276 (0%) Query: 18 INNYVQSDGTFSQPIPGLHIRRYSRTGVDWEKAFYLPSVLIVAQGVKTVRLEKETFQIGK 77 I + DGTF +PGL + R S T + Y P++ +VAQG K L + +F Sbjct: 26 IARHAPHDGTFETQLPGLKLIRCSETTLPM-PVVYEPTLCLVAQGRKQAVLGQTSFVYDP 84 Query: 78 SRMFMIPVALPVSLRTIQANAEQPFIGIGLTLDPEKIAEFVPKVYPRGLPSVQTRCAGYT 137 S + V LPV I+A+ ++P++ + L LD ++A+ + + Sbjct: 85 SSYLVASVDLPVMGSVIEASKDKPYLSMQLDLDTVELADLAIRYPSEQHAPINGPIGLLL 144 Query: 138 AEADTGIIHAVTRLIECLHHPSDAELLAPIVKDEILMRLLLSPIGIHIAEMGFADTSVRQ 197 + D GII A +RL+ L P D + L+P+ K EIL RL+ G + ++ A V Q Sbjct: 145 NKVDEGIIDAASRLLALLDTPRDIDALSPLAKREILYRLITGVGGAALRQIVQAGAHVNQ 204 Query: 198 VAKAIEWLRSNFDQPMKVSELASLSHMSVSVFHKHFKAVTSISPLKYQKELRLQEARNLM 257 +A++I W+R +F + V A ++ MS S FH HFKAVT++SPL+++ +LRLQEAR LM Sbjct: 205 IARSIVWIRKHFRETCSVEAGAEVAGMSRSTFHAHFKAVTNMSPLEFRTQLRLQEARRLM 264 Query: 258 LSKQMDAITASQLVGYASASQFSRDYRSYFGNPPRR 293 LS+ +DA TA VGYAS SQFSR+Y FG P + Sbjct: 265 LSEGLDAATAGFQVGYASPSQFSREYGRVFGMSPAK 300 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.134 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,160,730 Number of extensions: 784061 Number of successful extensions: 3859 Number of sequences better than 1.0e-05: 56 Number of HSP's gapped: 3827 Number of HSP's successfully gapped: 56 Length of query: 308 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 202 Effective length of database: 26,153,027 Effective search space: 5282911454 Effective search space used: 5282911454 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 116 (49.3 bits)