BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0094.0004.p (192 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAP0030 [Q] COG1335 Amidases related to nicotinamidase 202 2e-52 BS_ywoC [Q] COG1335 Amidases related to nicotinamidase 171 6e-43 ZyecD [Q] COG1335 Amidases related to nicotinamidase 146 2e-35 yecD [Q] COG1335 Amidases related to nicotinamidase 146 2e-35 ZyecD [Q] COG1335 Amidases related to nicotinamidase 146 2e-35 >CAP0030 [Q] COG1335 Amidases related to nicotinamidase Length = 187 Score = 202 bits (514), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 101/185 (54%), Positives = 129/185 (69%), Gaps = 2/185 (1%) Query: 1 MEKLFDPQKTALVVIDLQKWL-GNQYAPYSAEQVVSNAAVLADAFREQGSMVALVRVSSX 59 ME L + +KTALVVIDLQK + ++APY+ ++VV A+ L AF ++G+ V LV+VS+ Sbjct: 1 MENL-EIEKTALVVIDLQKGIVAGEHAPYTGKEVVEKASKLVKAFTDKGAFVVLVKVSTI 59 Query: 60 XXXXXXXXXXXTPAPALNLPEGWDEIVPEMRVAATDHIITKKQWGAFYGTELDLQLRRRE 119 + + PEGWD VPE+ + ITK+QWGAFYGT+LDLQLRRR Sbjct: 60 DGKDMLKPKLDSEINQIKRPEGWDSYVPEISNIKNTYAITKRQWGAFYGTDLDLQLRRRG 119 Query: 120 IDTLVLCGIATGLGVDTTAREAFMHGYQQIFAIDAMTGFSQAEHDYVKGHIFPRIGRTRT 179 IDT+VLCGI+TG+GVDTTAREAF HGY QIF DAMT S+ EH YV +IFPR+GR R Sbjct: 120 IDTMVLCGISTGIGVDTTAREAFQHGYNQIFVEDAMTARSREEHAYVCKYIFPRLGRIRN 179 Query: 180 TQEII 184 T+EI+ Sbjct: 180 TEEIL 184 >BS_ywoC [Q] COG1335 Amidases related to nicotinamidase Length = 189 Score = 171 bits (433), Expect = 6e-43, Method: Compositional matrix adjust. Identities = 91/182 (50%), Positives = 117/182 (64%), Gaps = 7/182 (3%) Query: 6 DPQKTALVVIDLQKWLGNQYAPYS-AEQVVSNAAVLADAFREQGSMVALVRVSSXXXXXX 64 D QKTALV+IDLQK + P + QVV NA L D FR+ ++ V V+ Sbjct: 8 DFQKTALVIIDLQKGI----VPIDQSGQVVPNAKKLVDEFRKHNGFISFVNVAFHDGADA 63 Query: 65 XXXXXXTPAPALN--LPEGWDEIVPEMRVAATDHIITKKQWGAFYGTELDLQLRRREIDT 122 PA + +P W E VPE+ V D+ +TK+QWGAF+GT+LDLQLRRR IDT Sbjct: 64 LKPQTDEPAQGGSGEMPADWAEFVPEIGVQDGDYTVTKRQWGAFFGTDLDLQLRRRGIDT 123 Query: 123 LVLCGIATGLGVDTTAREAFMHGYQQIFAIDAMTGFSQAEHDYVKGHIFPRIGRTRTTQE 182 +VLCGIAT +GV++TAREAF GYQ+IF DAM+ FS EH+ IFPRIG++RTT+E Sbjct: 124 IVLCGIATNIGVESTAREAFQLGYQRIFITDAMSTFSDEEHEATLRFIFPRIGKSRTTEE 183 Query: 183 II 184 + Sbjct: 184 FL 185 >ZyecD [Q] COG1335 Amidases related to nicotinamidase Length = 199 Score = 146 bits (369), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 81/185 (43%), Positives = 112/185 (60%), Gaps = 2/185 (1%) Query: 5 FDPQKTALVVIDLQKW-LGNQYAPYSAEQVVSNAAVLADAFREQGSMVALVRVS-SXXXX 62 + + TALVVIDLQ+ L P++A++VV+ A LA FR G V LVRV S Sbjct: 15 LNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKFRASGQPVFLVRVGWSADYA 74 Query: 63 XXXXXXXXTPAPALNLPEGWDEIVPEMRVAATDHIITKKQWGAFYGTELDLQLRRREIDT 122 P+PA LPE W + + +D I K+QWGAFYGT+L+LQLRRR IDT Sbjct: 75 EALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRRRGIDT 134 Query: 123 LVLCGIATGLGVDTTAREAFMHGYQQIFAIDAMTGFSQAEHDYVKGHIFPRIGRTRTTQE 182 +VLCGI+T +GV++TAR A+ G+ + A DA + S +H+ HI+PRI R R+ +E Sbjct: 135 IVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIARVRSVEE 194 Query: 183 IISTF 187 I++ Sbjct: 195 ILNAL 199 >yecD [Q] COG1335 Amidases related to nicotinamidase Length = 199 Score = 146 bits (369), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 81/185 (43%), Positives = 112/185 (60%), Gaps = 2/185 (1%) Query: 5 FDPQKTALVVIDLQKW-LGNQYAPYSAEQVVSNAAVLADAFREQGSMVALVRVS-SXXXX 62 + + TALVVIDLQ+ L P++A++VV+ A LA FR G V LVRV S Sbjct: 15 LNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKFRASGQPVFLVRVGWSADYA 74 Query: 63 XXXXXXXXTPAPALNLPEGWDEIVPEMRVAATDHIITKKQWGAFYGTELDLQLRRREIDT 122 P+PA LPE W + + +D I K+QWGAFYGT+L+LQLRRR IDT Sbjct: 75 EALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRRRGIDT 134 Query: 123 LVLCGIATGLGVDTTAREAFMHGYQQIFAIDAMTGFSQAEHDYVKGHIFPRIGRTRTTQE 182 +VLCGI+T +GV++TAR A+ G+ + A DA + S +H+ HI+PRI R R+ +E Sbjct: 135 IVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIARVRSVEE 194 Query: 183 IISTF 187 I++ Sbjct: 195 ILNAL 199 Score = 146 bits (369), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 81/185 (43%), Positives = 112/185 (60%), Gaps = 2/185 (1%) Query: 5 FDPQKTALVVIDLQKW-LGNQYAPYSAEQVVSNAAVLADAFREQGSMVALVRVS-SXXXX 62 + + TALVVIDLQ+ L P++A++VV+ A LA FR G V LVRV S Sbjct: 15 LNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKFRASGQPVFLVRVGWSADYA 74 Query: 63 XXXXXXXXTPAPALNLPEGWDEIVPEMRVAATDHIITKKQWGAFYGTELDLQLRRREIDT 122 P+PA LPE W + + +D I K+QWGAFYGT+L+LQLRRR IDT Sbjct: 75 EALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRRRGIDT 134 Query: 123 LVLCGIATGLGVDTTAREAFMHGYQQIFAIDAMTGFSQAEHDYVKGHIFPRIGRTRTTQE 182 +VLCGI+T +GV++TAR A+ G+ + A DA + S +H+ HI+PRI R R+ +E Sbjct: 135 IVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIARVRSVEE 194 Query: 183 IISTF 187 I++ Sbjct: 195 ILNAL 199 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.134 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 12,421,565 Number of extensions: 404927 Number of successful extensions: 820 Number of sequences better than 1.0e-05: 29 Number of HSP's gapped: 808 Number of HSP's successfully gapped: 29 Length of query: 192 Length of database: 38,378,431 Length adjustment: 101 Effective length of query: 91 Effective length of database: 26,729,697 Effective search space: 2432402427 Effective search space used: 2432402427 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 113 (48.1 bits)