BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0091.0001.p (415 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA5415 [E] COG0112 Glycine/serine hydroxymethyltransferase 507 e-143 AGc2156 [E] COG0112 Glycine/serine hydroxymethyltransferase 505 e-143 SMc01770 [E] COG0112 Glycine/serine hydroxymethyltransferase 504 e-142 RSc0729 [E] COG0112 Glycine/serine hydroxymethyltransferase 502 e-142 mlr8400 [E] COG0112 Glycine/serine hydroxymethyltransferase 498 e-141 >PA5415 [E] COG0112 Glycine/serine hydroxymethyltransferase Length = 417 Score = 507 bits (1306), Expect = e-143, Method: Compositional matrix adjust. Identities = 250/411 (60%), Positives = 308/411 (74%), Gaps = 2/411 (0%) Query: 2 EQLRKSDPAVLEAMGLELSRQRANIELIASENIVSEAVMEAMGSVLTNKYAEGYPGKRYY 61 +Q+R D +L AM E +RQ ++ELIASEN S+ VM+A GS LTNKYAEGYPGKRYY Sbjct: 6 DQIRGYDDELLAAMDAEEARQEDHLELIASENYTSKRVMQAQGSGLTNKYAEGYPGKRYY 65 Query: 62 GGCEDVDIVENLARDRAKQLFGADHANVQPHSGAQANMAVYLAALNPGDTVLGMNLAHGG 121 GGCE VD VE LA DRA+QLFGAD+ANVQPHSG+ AN AVYLA LN GDT+LGM+LAHGG Sbjct: 66 GGCEHVDKVERLAIDRARQLFGADYANVQPHSGSSANAAVYLALLNAGDTILGMSLAHGG 125 Query: 122 HLTHGSPVNASGLLYNFVAYGVQEDTFLIDYDEVRKAAFKHRPKLIVAGASAYPRTIDFE 181 HLTHG+ V++SG LYN V YG+ T LIDYDEV + A +H+PK+IVAG SAY +T+DF Sbjct: 126 HLTHGAKVSSSGKLYNAVQYGLDTATGLIDYDEVERLAVEHKPKMIVAGFSAYSKTLDFP 185 Query: 182 ALGSIANDVGALFMVDMAHIAGLVAAGLHPSPVPHAHFVTTTTHKTLRGPRGGMILCR-- 239 +IA+ VGAL VDMAH+AGLVAAGL+P+P+P A VTTTTHKTLRGPRGG+IL R Sbjct: 186 RFRAIADKVGALLFVDMAHVAGLVAAGLYPNPIPFADVVTTTTHKTLRGPRGGLILARAN 245 Query: 240 QPWAAAIDKAVFPGSQGGPLMHVIASKAVSFGEALQPSFRTYAENVVKNAKVLAETLIGE 299 + ++ AVFPG+QGGPLMHVIA+KAV F EAL+P F+ Y V++NAK +AE IG Sbjct: 246 EEIEKKLNSAVFPGAQGGPLMHVIAAKAVCFKEALEPGFKDYQAQVIRNAKAMAEVFIGR 305 Query: 300 GVNIVSGGTDNHLMLLDTRNLNITGKDAEKVLDSIGITVNKNAIPFDPTSPFVTSGIRIG 359 G ++VSGGTDNHLML+ +TGK+A+ L +GITVNKNA+P DP SPFVTSGIRIG Sbjct: 306 GYDVVSGGTDNHLMLISLVRQGLTGKEADAALGRVGITVNKNAVPNDPQSPFVTSGIRIG 365 Query: 360 TPAVTSRGMNEQAMVTIGRIIASVLKNPKDEGKLAEAAREVAALTEQYPIY 410 TPA+T+RG+ E + I +L + D A+ A +VA L +P+Y Sbjct: 366 TPAITTRGLQEAQSRELAGWICDILDHLGDADVEAKVATQVAGLCADFPVY 416 >AGc2156 [E] COG0112 Glycine/serine hydroxymethyltransferase Length = 301 Score = 505 bits (1301), Expect = e-143, Method: Compositional matrix adjust. Identities = 0/416 (0%), Positives = 0/416 (0%), Gaps = 8/416 (1%) Query: 4 LRKSDPAVLEAMGLELSRQRANIELIASENIVSEAVMEAMGSVLTNKYAEGYPGKRYYGG 63 + + + ++ +E + + A Sbjct: 74 INRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILAVNKVDNVQEKA 133 Query: 64 CEDVDIVENLARDRAKQLFGADHANVQPHSGAQANMAVYLAALNPGDTVLGMNLAHGGHL 123 + ++ + A + +L + + Sbjct: 134 DLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHFPEDYITDRSQRFM 193 Query: 124 THGSPVNASGLLYNFVAYGVQEDTFLIDYDEVRKAAFKHRPKLIVAGASAYPRTIDFEAL 183 R + L+ + A Sbjct: 194 ASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDINGLILVEREGQKKMVIGNKGAK 253 Query: 184 GSIANDVGALFMVDMAHIAGLVAAGLHPSPVPHAHFVTTTTHKTLRGPRGGMILCR-QPW 242 Sbjct: 254 313 Query: 243 AAAIDKAVFPGSQGGPLMHVIASKAVSFGEALQPSFRTYAENVVKNAKVLAETLIGEGVN 302 Sbjct: 314 373 Query: 303 IVSGGTDNHLMLLDTRNLNITGKDAEKVLDSIGITVNKNAIPFDPTSPFVTSGIRIGTPA 362 Sbjct: 374 433 Query: 363 VTSRGMNEQAMVTIGRIIASVLKNPK----DEG--KLAEAARE-VAALTEQYPIYP 411 Sbjct: 434 489 >SMc01770 [E] COG0112 Glycine/serine hydroxymethyltransferase Length = 431 Score = 504 bits (1297), Expect = e-142, Method: Compositional matrix adjust. Identities = 253/415 (60%), Positives = 302/415 (72%), Gaps = 8/415 (1%) Query: 4 LRKSDPAVLEAMGLELSRQRANIELIASENIVSEAVMEAMGSVLTNKYAEGYPGKRYYGG 63 L SDP + A+ EL RQR IELIASENIVS AV+EA GS++TNKYAEGYPGKRYYGG Sbjct: 14 LADSDPEIFGAIEKELGRQRHEIELIASENIVSRAVLEAQGSIMTNKYAEGYPGKRYYGG 73 Query: 64 CEDVDIVENLARDRAKQLFGADHANVQPHSGAQANMAVYLAALNPGDTVLGMNLAHGGHL 123 C+ VDI E LA +RAK+LFG + ANVQP+SG+Q N AV+LA L PGDT +G++L GGHL Sbjct: 74 CQYVDIAEALAIERAKKLFGVNFANVQPNSGSQMNQAVFLALLQPGDTFMGLDLNSGGHL 133 Query: 124 THGSPVNASGLLYNFVAYGVQEDTFLIDYDEVRKAAFKHRPKLIVAGASAYPRTIDFEAL 183 THGSPVN SG +N V+YGV+ED L+D DEV + A + +PKLI+AG +AY R D++ Sbjct: 134 THGSPVNMSGKWFNVVSYGVREDDHLLDMDEVARKAREQKPKLIIAGGTAYSRIWDWKRF 193 Query: 184 GSIANDVGALFMVDMAHIAGLVAAGLHPSPVPHAHFVTTTTHKTLRGPRGGMILCR-QPW 242 IA++VGA MVDMAHIAGLVA G HPSP PH H TTTTHK+LRGPRGGMIL + Sbjct: 194 REIADEVGAWLMVDMAHIAGLVAGGQHPSPFPHCHVATTTTHKSLRGPRGGMILTNDEEI 253 Query: 243 AAAIDKAVFPGSQGGPLMHVIASKAVSFGEALQPSFRTYAENVVKNAKVLAETLIGEGVN 302 A I+ AVFPG QGGPLMHVIA+KAV+ GEALQPSF+ YA VVKNA+ LAETL G++ Sbjct: 254 AKKINSAVFPGLQGGPLMHVIAAKAVALGEALQPSFKDYAAQVVKNARTLAETLKANGLD 313 Query: 303 IVSGGTDNHLMLLDTRNLNITGKDAEKVLDSIGITVNKNAIPFDPTSPFVTSGIRIGTPA 362 IVSGGTDNHLML+D R N TGK AE L +T NKN IPFDP PFVTSG+R+G PA Sbjct: 314 IVSGGTDNHLMLVDLRKKNATGKRAEAALGRAYVTCNKNGIPFDPEKPFVTSGVRLGAPA 373 Query: 363 VTSRGMNEQAMVTIGRIIASVLKNPK----DEGKLAEAA---REVAALTEQYPIY 410 T+RG E +G +I VL K DEG A A +V LT+++P+Y Sbjct: 374 GTTRGFKEAEFKEVGELIVEVLDGLKAANSDEGNAAVEAGVREKVIKLTDRFPMY 428 >RSc0729 [E] COG0112 Glycine/serine hydroxymethyltransferase Length = 415 Score = 502 bits (1293), Expect = e-142, Method: Compositional matrix adjust. Identities = 249/405 (61%), Positives = 303/405 (74%), Gaps = 5/405 (1%) Query: 8 DPAVLEAMGLELSRQRANIELIASENIVSEAVMEAMGSVLTNKYAEGYPGKRYYGGCEDV 67 DP V A+ E RQ +IELIASEN S AVM A GS LTNKYAEGYPGKRYYGGCE V Sbjct: 13 DPEVFAAIQQENQRQEDHIELIASENYTSPAVMAAQGSQLTNKYAEGYPGKRYYGGCEYV 72 Query: 68 DIVENLARDRAKQLFGADHANVQPHSGAQANMAVYLAALNPGDTVLGMNLAHGGHLTHGS 127 D+VE LA DR KQLFGA+ ANVQP+SG+QAN V+ A L PGDT++GM+LA GGHLTHG Sbjct: 73 DVVEQLAIDRVKQLFGAEAANVQPNSGSQANQGVFFAMLKPGDTIMGMSLAEGGHLTHGM 132 Query: 128 PVNASGLLYNFVAYGV--QEDTFLIDYDEVRKAAFKHRPKLIVAGASAYPRTIDFEALGS 185 +N SG +N V+YG+ QED IDYD + A + +PKLI+AGASA+ IDFE + Sbjct: 133 ALNMSGKWFNVVSYGLNAQED---IDYDALEALAQEKKPKLIIAGASAFALRIDFERIAK 189 Query: 186 IANDVGALFMVDMAHIAGLVAAGLHPSPVPHAHFVTTTTHKTLRGPRGGMILCRQPWAAA 245 +A VGA FMVDMAH AGL+AAG++P+PVPHA FVTTTTHK+LRGPRGG+IL + A Sbjct: 190 VAKAVGAYFMVDMAHYAGLIAAGVYPNPVPHADFVTTTTHKSLRGPRGGVILMKAEHEKA 249 Query: 246 IDKAVFPGSQGGPLMHVIASKAVSFGEALQPSFRTYAENVVKNAKVLAETLIGEGVNIVS 305 I+ A+FPG QGGPLMHVIA KAV+F EA P+F+ Y E VVKNA+ +AETL+ G+ IVS Sbjct: 250 INSAIFPGIQGGPLMHVIAGKAVAFKEAQSPTFKAYQEQVVKNARAMAETLMARGLRIVS 309 Query: 306 GGTDNHLMLLDTRNLNITGKDAEKVLDSIGITVNKNAIPFDPTSPFVTSGIRIGTPAVTS 365 G T++H+ML+D R +ITGK+AEKVL ITVNKNAIP DP PFVTSGIR+G+PA+T+ Sbjct: 310 GRTESHVMLVDLRAKSITGKEAEKVLGDAHITVNKNAIPNDPEKPFVTSGIRLGSPAMTT 369 Query: 366 RGMNEQAMVTIGRIIASVLKNPKDEGKLAEAAREVAALTEQYPIY 410 RG E V + +IA VL NP DE +A +VA LT+Q+P+Y Sbjct: 370 RGFKEGEAVKVAHLIADVLDNPHDEANIAAVRAKVAELTKQFPVY 414 >mlr8400 [E] COG0112 Glycine/serine hydroxymethyltransferase Length = 301 Score = 498 bits (1283), Expect = e-141, Method: Compositional matrix adjust. Identities = 0/418 (0%), Positives = 0/418 (0%), Gaps = 8/418 (1%) Query: 4 LRKSDPAVLEAMGLELSRQRANIELIASENIVSEAVMEAMGSVLTNKYAEGYPGKRYYGG 63 + KS R + V T Sbjct: 19 VGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLM 78 Query: 64 CEDVDIVENLARDRAKQLFGADHANVQPHSGAQANMAVYLAALNPGDTVLGMNLAHGGHL 123 + + G + L A Sbjct: 79 NKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPH 138 Query: 124 THGSPVNASGLLYNFVAYGVQEDTFLIDYDEVRKAAFKHRPKLIVAGASAYPRTIDFEAL 183 + L ++ + I + R + E + Sbjct: 139 LQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEII 198 Query: 184 GSIANDVGALFMVDMAHIAGLVAAGLHPSPVPHAHFVTTTTHKTLRGPRGGMILCR-QPW 242 + + + + G Sbjct: 199 REKLMRFLGAELPYSVTVEIERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIG 258 Query: 243 AAAIDKAVFPGSQGGPLMHVIASKAVSFGEALQPSFRTYAENVVKNAKVLAETLIGEGVN 302 Sbjct: 259 318 Query: 303 IVSGGTDNHLMLLDTRNLNITGKDAEKVLDSIGITVNKNAIPFDPTSPFVTSGIRIGTPA 362 Sbjct: 319 378 Query: 363 VTSRGMNEQAMVTIGRIIASVLKNPK----DEGK---LAEAAREVAALTEQYPIYPGL 413 Sbjct: 379 436 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.134 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,372,914 Number of extensions: 1361473 Number of successful extensions: 7852 Number of sequences better than 1.0e-05: 30 Number of HSP's gapped: 7782 Number of HSP's successfully gapped: 30 Length of query: 415 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 306 Effective length of database: 25,807,025 Effective search space: 7896949650 Effective search space used: 7896949650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)