BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0087.0005.p (490 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin1658 [GEPR] COG0477 Permeases of the major facilitator superf... 346 5e-95 lin0519 [GEPR] COG0477 Permeases of the major facilitator superf... 328 2e-89 BS_lmrB [GEPR] COG0477 Permeases of the major facilitator superf... 290 4e-78 L132251 [GEPR] COG0477 Permeases of the major facilitator superf... 239 1e-62 BH0554 [GEPR] COG0477 Permeases of the major facilitator superfa... 168 2e-41 >lin1658 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 489 Score = 346 bits (888), Expect = 5e-95, Method: Compositional matrix adjust. Identities = 179/466 (38%), Positives = 288/466 (61%), Gaps = 13/466 (2%) Query: 29 IMAILIIGSMVGLLNQSSLNTALPNIMGDFGLSTSTAQWLTTAFALVTGIVVPITAFLIQ 88 I+ ++++G+ V +LNQ+ +N ALP+IM DFG++ S QWL+T F LV G+++P+TAFLI+ Sbjct: 13 ILIVMLMGAFVTILNQTLMNVALPSIMKDFGITASQGQWLSTGFMLVNGVMIPMTAFLIE 72 Query: 89 RFTTKQVFVFAMGMLTAGTLLCAVSPNFGLLLTGRMVQAVGVGIMLPLVQTLTFILIPVA 148 RFTT+Q+++FAM GT + + ++ +L+ GRMVQA+G GI++PL+ + L P+ Sbjct: 73 RFTTRQLYLFAMITFAIGTAIGGFATDYTMLIAGRMVQAIGAGIVMPLLTVVVLNLFPME 132 Query: 149 KRGFTMGMIGLAINFAPAIGPVLNGWLVEDHSWRLLFYALFPCALLITIVGLFFVKNVTP 208 +RG MG+IGLA+NFAPAIGP L+GW+V+ + WR LF+ + P A+L +V +F +KNV Sbjct: 133 RRGRAMGLIGLAMNFAPAIGPTLSGWIVQQYDWRNLFFIIIPFAILDIVVAIFLLKNVGK 192 Query: 209 QVKSKIDALSMTLSSLGFGGVLYGFSVSGSKGWGSTEVLISLVIGFIALGLFVWRQLTME 268 + K+D L + +S++GFG +L GFS +G W + +V +++G + LG+F+ Q + + Sbjct: 193 RTFPKLDILGVIMSTVGFGSLLLGFSNAGDHDWLTWKVAGFIILGLVVLGIFIRYQSSNK 252 Query: 269 NPLLEIRVFTSPAFTMATVXXXXXXXXXXXXXXXXPLYMQTMLGYSSLKSGLMLLPGAIL 328 PLL +VF P F + T P+++QT+ G+S L+SGL+LLPGA++ Sbjct: 253 APLLNFKVFKYPTFALTTAISFFVVMGLFGGMLLLPIFLQTVRGFSPLESGLVLLPGALV 312 Query: 329 ICVMSPVAGKLFDKLGARMXXXXXXXXXXXXXXXXXXXXENTSYAFLMIGYSLRMIGVSL 388 ++SPV G +FD+ GA+ E+T+ F++I ++R G+++ Sbjct: 313 TAILSPVTGVMFDRFGAKYLSLVGLIIMAGSTFMFTNLDESTTLTFIIIVQTIRSAGMAM 372 Query: 389 TLLPVITSGMNSLAPDLIRHGIPVNTTLRTVAGSIGTALLVTIMTHSGGGLGQT---SDV 445 ++P+ T+ +NSL L HG + T+R VAGSIGTA L+T+M+ S +DV Sbjct: 373 VMMPLQTAALNSLPLKLASHGSAMFNTMRQVAGSIGTAALITVMSKSAASFADKLGPADV 432 Query: 446 ----------HGLIHGMNVASMVTAGSAVIALILAFFIKRKEAPVK 481 H LIHG+ A +V +V+A ILA FI++ + + Sbjct: 433 IGKTKTEVANHVLIHGIETAFLVAGILSVVACILALFIQKNRSAMD 478 >lin0519 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 471 Score = 328 bits (840), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 180/469 (38%), Positives = 280/469 (59%), Gaps = 12/469 (2%) Query: 16 GNRMEKIPKGTILIMAILIIGSMVGLLNQSSLNTALPNIMGDFGLSTSTAQWLTTAFALV 75 G ++ IP I+A ++ +GL ++++LN AL +++ F +S++T QWLTT + L Sbjct: 8 GQKIRPIP-----IIASFLMAGFIGLFSETALNMALSDLIQVFDISSATVQWLTTGYLLT 62 Query: 76 TGIVVPITAFLIQRFTTKQVFVFAMGMLTAGTLLCAVSPNFGLLLTGRMVQAVGVGIMLP 135 GI+VPI+ L+Q FTT+ +F A+ AGTL+ A+SP F +L+ GR+VQAVG ++LP Sbjct: 63 LGILVPISGLLLQWFTTRGLFFTAVSFSIAGTLIAALSPTFAMLMIGRVVQAVGTALLLP 122 Query: 136 LVQTLTFILIPVAKRGFTMGMIGLAINFAPAIGPVLNGWLVEDHSWRLLFYALFPCALLI 195 L+ ++ P KRG MGMIGL I FAPA+GP ++G ++E+ +W +F+ P ++ Sbjct: 123 LMFNTILLIFPEHKRGSAMGMIGLVIMFAPAVGPTISGLILENLTWNWIFWISLPFLIIA 182 Query: 196 TIVGLFFVKNVTPQVKSKIDALSMTLSSLGFGGVLYGFSVSGSKGWGSTEVLISLVIGFI 255 + G+ F++NV+ K KID LS+ LS+LGFGGV++ FS +G GWGS VL+S+++G I Sbjct: 183 LLFGMKFMQNVSVVTKPKIDILSIILSTLGFGGVVFAFSSAGENGWGSATVLVSIIVGGI 242 Query: 256 ALGLFVWRQLTMENPLLEIRVFTSPAFTMATVXXXXXXXXXXXXXXXXPLYMQTMLGYSS 315 ALGLFVWRQLTME PL++++VF P FT+ + PLY+Q L ++ Sbjct: 243 ALGLFVWRQLTMEKPLMDLKVFKYPMFTLGLILVFISFMMILSTMILLPLYLQNSLALAA 302 Query: 316 LKSGLMLLPGAILICVMSPVAGKLFDKLGARMXXXXXXXXXXXXXXXXXXXXENTSYAFL 375 +GL+LLPG +L +MSP G+LFD G R TS + Sbjct: 303 FSAGLVLLPGGVLNGLMSPFTGRLFDAYGPRALVIPGFIVAVIALFFLTRIEVGTSALTI 362 Query: 376 MIGYSLRMIGVSLTLLPVITSGMNSLAPDLIRHGIPVNTTLRTVAGSIGTALLVTIMT-- 433 ++ +S+ MIG+S+ ++P T+G+N L P L G + TL+ V+G+IGTA+ +TIM+ Sbjct: 363 IVLHSVLMIGISMVMMPAQTNGLNQLPPKLYPDGTAIMNTLQQVSGAIGTAVAITIMSAG 422 Query: 434 -----HSGGGLGQTSDVHGLIHGMNVASMVTAGSAVIALILAFFIKRKE 477 + G+G + L G+ A + A I L+ + FI++ + Sbjct: 423 QKAYMETAQGVGPEQMIASLTAGIQNAFVFGLIMACIGLLCSLFIRKAK 471 >BS_lmrB [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 479 Score = 290 bits (741), Expect = 4e-78, Method: Compositional matrix adjust. Identities = 167/461 (36%), Positives = 264/461 (57%), Gaps = 14/461 (3%) Query: 29 IMAILIIGSMVGLLNQSSLNTALPNIMGDFGLSTSTAQWLTTAFALVTGIVVPITAFLIQ 88 IM L++ +G+ ++++LN AL ++M + ++ +T QWLTT + LV GI+VP++ L+Q Sbjct: 18 IMISLLLAGFIGMFSETALNIALTDLMKELNITAATVQWLTTGYLLVLGILVPVSGLLLQ 77 Query: 89 RFTTKQVFVFAMGMLTAGTLLCAVSPNFGLLLTGRMVQAVGVGIMLPLVQTLTFILIPVA 148 FTT+Q+F ++ GT + A++P+F LL R+VQA+G G++LPL+ ++ P Sbjct: 78 WFTTRQLFTVSLIFSILGTFIAALAPSFSFLLAARIVQALGTGLLLPLMFNTILVIFPPH 137 Query: 149 KRGFTMGMIGLAINFAPAIGPVLNGWLVEDHSWRLLFYALFPCALLITIVGLFFVKNVTP 208 KRG MG IGL I FAPAIGP +G ++E +W +F+ P +L + G+ +++NV+ Sbjct: 138 KRGAAMGTIGLVIMFAPAIGPTFSGLVLEHLNWHWIFWISLPFLVLALVFGIAYMQNVSE 197 Query: 209 QVKSKIDALSMTLSSLGFGGVLYGFSVS--GSKGWGSTEVLISLVIGFIALGLFVWRQLT 266 K KID LS+ LS++GFGG+++GFS + GS GW S V++SL++G + L LF RQLT Sbjct: 198 TTKPKIDVLSIILSTIGFGGIVFGFSNAGEGSGGWSSPTVIVSLIVGVVGLILFSIRQLT 257 Query: 267 MENPLLEIRVFTSPAFTMATVXXXXXXXXXXXXXXXXPLYMQTMLGYSSLKSGLMLLPGA 326 M+ P++ +R F P F + + P+Y+Q L ++ SGL+LLPG Sbjct: 258 MKQPMMNLRAFKYPMFILGVIMVFICMMVILSSMLLLPMYLQGGLVLTAFASGLVLLPGG 317 Query: 327 ILICVMSPVAGKLFDKLGARMXXXXXXXXXXXXXXXXXXXXENTSYAFLMIGYSLRMIGV 386 IL MSPV G+LFDK G + ++ ++I ++ MIG+ Sbjct: 318 ILNGFMSPVTGRLFDKYGPKWLVIPGFVIVTVVLWFFSNVTTTSTAVLIIILHTCLMIGI 377 Query: 387 SLTLLPVITSGMNSLAPDLIRHGIPVNTTLRTVAGSIGTALLVTIMTHSGGGLGQTSDVH 446 S+ ++P T+G+N L + G + TL+ +AG+IGTA+ V+IM + G S V Sbjct: 378 SMIMMPAQTNGLNQLPREFYPDGTAIMNTLQQMAGAIGTAVAVSIM--AAGQHDYMSTVK 435 Query: 447 GLIHGMNVASMVTAG----------SAVIALILAFFIKRKE 477 + +TAG A+I LI AFF+KR + Sbjct: 436 NPADPAVIPQALTAGVQHAFVFAMIVAIIGLIGAFFMKRVK 476 >L132251 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 514 Score = 239 bits (609), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 139/413 (33%), Positives = 228/413 (55%), Gaps = 13/413 (3%) Query: 32 ILIIGSMVGLLNQSSLNTALPNIMGDFGLSTSTAQWLTTAFALVTGIVVPITAFLIQRFT 91 ++++G+ +L Q+SL TA+P +M DF +S STAQ TT F L GI+VPI+A+L +F Sbjct: 26 LILVGTFGAMLMQTSLGTAIPTLMNDFNISMSTAQQATTWFLLANGIMVPISAYLATKFP 85 Query: 92 TKQVFVFAMGMLTAGTLLCAVSP--NFGLLLTGRMVQAVGVGIMLPLVQTLTFILIPVAK 149 T+ ++V A L G + +P ++ + L GR++QA+ VGI +PL+Q + P + Sbjct: 86 TRWLYVTAYATLFIGMFVAYSAPTSSWWVFLAGRILQAIAVGITMPLMQVALVNMFPAKQ 145 Query: 150 RGFTMGMIGLAINFAPAIGPVLNGWLVEDH----------SWRLLFYALFPCALLITIVG 199 G MG+ G+ + APA+GP GWL+ SWR +F ++ ++ Sbjct: 146 MGAIMGVAGVVLVLAPALGPTFAGWLITHKFNLIFWTFAASWRTIFLIPMIVVGVVFLIS 205 Query: 200 LFFVKNVTPQVKSKIDALSMTLSSLGFGGVLYGFSVSGSKGWGSTEVLISLVI-GFIALG 258 +F +++V P K+D LS+ LS +GFG L+GF+ + GWG +I+ ++ G I + Sbjct: 206 IFAMRDVIPNRPMKLDFLSVLLSVVGFGAFLWGFTNVATDGWGKFNTVIAPIVGGLIIIF 265 Query: 259 LFVWRQLTMENPLLEIRVFTSPAFTMATVXXXXXXXXXXXXXXXXPLYMQTMLGYSSLKS 318 LF RQL M++P L+IRVF FT+ T+ PLY+Q + G S+ S Sbjct: 266 LFGLRQLKMDDPFLDIRVFKIRQFTLVTLALALSTMAMMGVEMMLPLYLQDVHGLSAFNS 325 Query: 319 GLMLLPGAILICVMSPVAGKLFDKLGARMXXXXXXXXXXXXXXXXXXXXENTSYAFLMIG 378 GL+LLPG++++ V++P+AG ++D++G++ T F + Sbjct: 326 GLVLLPGSLMMAVVAPLAGGVYDRIGSKRLAVIGFLVLALGTIPFMFFTATTPDHFDTLL 385 Query: 379 YSLRMIGVSLTLLPVITSGMNSLAPDLIRHGIPVNTTLRTVAGSIGTALLVTI 431 Y LRM+GV + ++P+ TS MN++ + + N T R VA S+ ALL ++ Sbjct: 386 YGLRMVGVGMVMMPLTTSAMNAVPVNEVAQATASNNTARQVASSVVVALLSSV 438 >BH0554 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 474 Score = 168 bits (426), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 123/462 (26%), Positives = 215/462 (46%), Gaps = 6/462 (1%) Query: 19 MEKIPKGTILIMAILIIGSMVGLLNQSSLNTALPNIMGDFGLSTSTAQWLTTAFALVTGI 78 M+ IPK +++ A+L GS +LN S LN A+P +M F W+ T F + G+ Sbjct: 1 MKNIPKKWMVVCAVLF-GSFTMILNNSMLNPAIPQLMNVFEADAVATGWVITIFMVAMGM 59 Query: 79 VVPITAFLIQRFTTKQVFVFAMGMLTAGTLLCAVSPNFGLLLTGRMVQAVGVGIMLPLVQ 138 +P+T +L + K+ ++ +G+ G+LL A+S N L+ R +Q +G G+M+PL Sbjct: 60 TMPLTGYLGDKIGKKEAYILGLGIFVLGSLLGALSWNLPSLIVFRGLQGIGGGVMMPLSM 119 Query: 139 TLTFILIPVAKRGFTMGMIGLAINFAPAIGPVLNGWLVEDHSWRLLFYALFPCALLITIV 198 TL F P +RG G+ G+A AP IGP L G++VE +W+ LF P LL I Sbjct: 120 TLIFDAFPRNERGLATGVWGVASMMAPTIGPTLGGFIVETSNWKYLFLVNIPFGLLGIIA 179 Query: 199 GLFFVKNVTPQVKSKIDALSMTLSSLGFGGVLYGFSVSGSKGWGSTEVLISLVIGFIALG 258 + ++ + + K+D + G G +L F S TE + +++ AL Sbjct: 180 AVIYLPKIARSGQIKLDRWGFLFVTAGVGSILLAFG-RMSDLTHLTEWINGVLLLLGALC 238 Query: 259 LFVWRQLTM--ENPLLEIRVFTSPAFTMATVXXXXXXXXXXXXXXXXPLYMQTMLGYSSL 316 L+V+ Q+ E PLL++ +F PA++++ PL +Q + Y + Sbjct: 239 LYVFVQVEKRAEQPLLDLSLFRIPAYSLSIWVAGISSIGLFAGIFLVPLLLQQVYDYGPI 298 Query: 317 KSGLMLLPGAILICVMSPVAGKLFDKLGARMXXXXXXXXXXXXXXXXXXXXENTSYAFLM 376 +GL+ LP A+ + + G++ DK G T ++ Sbjct: 299 MTGLVFLPSALFTGLTMSIGGRMLDKRGPSGIMTAGMMIAAVGTFALGYLHLETGLWYIF 358 Query: 377 IGYSLRMIGVSLTLLPVITSGMNSLAPDLIRHGIPVNTTLRTVAGSIGTALLVTIMTHSG 436 ++R +G+ LT +P T+GMN++ LI G +N LR ++ + G + Sbjct: 359 AWMAIRGVGMGLTTMPATTTGMNAIPEGLISRGSAMNNVLRQMSSAFGIVFISVFFEVRR 418 Query: 437 GGLG--QTSDVHGLIHGMNVASMVTAGSAVIALILAFFIKRK 476 G L TS + +N + +++ A+++++K Sbjct: 419 GQLALVSTSFEEATLQAINEGFFIVGFLTALSIPAAYWLEKK 460 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.326 0.140 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,872,122 Number of extensions: 1149052 Number of successful extensions: 4732 Number of sequences better than 1.0e-05: 104 Number of HSP's gapped: 4630 Number of HSP's successfully gapped: 104 Length of query: 490 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 380 Effective length of database: 25,691,691 Effective search space: 9762842580 Effective search space used: 9762842580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 118 (50.1 bits)