BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0081.0026.p (207 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0128 [M] COG5632 N-acetylmuramoyl-L-alanine amidase 123 3e-28 >lin0128 [M] COG5632 N-acetylmuramoyl-L-alanine amidase Length = 289 Score = 123 bits (308), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 60/126 (47%), Positives = 80/126 (63%), Gaps = 7/126 (5%) Query: 4 LIERCYACGVPILITQGLRTAAEQDALYAQGRTKSGAIVTNARGGQSYHNYGLAVDFALL 63 +I++ G+ + + QG R+ AEQ+ALYAQGRTK GAIVTNA+GGQS HNYG+AVD L Sbjct: 32 VIKKMAKEGIYLCVAQGYRSTAEQNALYAQGRTKPGAIVTNAKGGQSNHNYGVAVDLCLY 91 Query: 64 LPNGSSVSWDMARDGDNDQAADWLEVVQQAKRLGFEWGGDWTSLKDYPHFQMSFGLTLSK 123 +G V W+ + W +VV K GF+WGGDW S KDYPHF++ ++ K Sbjct: 92 TNDGKDVIWE-------STTSRWKKVVAAMKAEGFKWGGDWKSFKDYPHFELCDAVSGEK 144 Query: 124 LRAGAQ 129 + A Q Sbjct: 145 IPAATQ 150 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.133 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 14,977,825 Number of extensions: 621140 Number of successful extensions: 1227 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 1227 Number of HSP's successfully gapped: 1 Length of query: 207 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 105 Effective length of database: 26,614,363 Effective search space: 2794508115 Effective search space used: 2794508115 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 113 (48.1 bits)