BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0081.0015.p (354 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yqbT [S] COG3299 Uncharacterized homolog of phage Mu protein ... 253 3e-67 BS_xkdT [S] COG3299 Uncharacterized homolog of phage Mu protein ... 253 3e-67 lin1287 [S] COG3299 Uncharacterized homolog of phage Mu protein ... 198 1e-50 NMA1323 [S] COG3299 Uncharacterized homolog of phage Mu protein ... 82 2e-15 NMA1826 [S] COG3299 Uncharacterized homolog of phage Mu protein ... 67 3e-11 >BS_yqbT [S] COG3299 Uncharacterized homolog of phage Mu protein gp47 Length = 348 Score = 253 bits (647), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 140/350 (40%), Positives = 204/350 (58%), Gaps = 11/350 (3%) Query: 3 VYEDQTFEALLGRMLDRVPSSLDKREGSIIYDXXXXXXXXXXQMYIELDVNSNLFFADTA 62 ++EDQT+E+++ RML+ + + +DKREGS+IY+ + YI LD L F+DTA Sbjct: 1 MFEDQTYESIMDRMLNSISADIDKREGSVIYNALAPVAAELAKSYIWLDTVLELVFSDTA 60 Query: 63 TGEYLERSIAWSGITRRAASKAELKAMFYNSASGLLDVPLGSRFSLDLLHYTAVEKLAPG 122 GE+L+R +GI R AA+KA F + +P+GSRF +D L++ + A G Sbjct: 61 QGEFLDRRATEAGIERTAATKAVRAGEFTEGVT----IPVGSRFYVDNLYF---QYTADG 113 Query: 123 TYRLESETAGEEGNRYFG--SLLPIDYIPVLARGEITALLIPGENAEDDEALRQRYLESV 180 T LE ETAGE GN +LL +D IP L + + +LIPG EDD++LR RY V Sbjct: 114 T--LECETAGEAGNANISGQNLLSLDTIPGLQKAIVKEILIPGREEEDDDSLRARYFTRV 171 Query: 181 RRPSTSGNKYHYMEWALQIDGVGGARVFPLWNGPKTVKVVIVDAAKRPASELLVSQVQQY 240 RR + S NK HY +WA ++DGVG +VFPLWNG TVK+V+ +A PAS++L+S+V+ Y Sbjct: 172 RREAVSANKAHYKQWAEEVDGVGKVKVFPLWNGDGTVKIVVTNANLEPASDILISKVKNY 231 Query: 241 IDPVSGQGEGQXXXXXXXXXXXXXGKSISVSAKVTLASGYSLQEVIDAFKEILEKYRKEK 300 IDP GQGEGQ K + +SA+V S+ +V + + K+ Sbjct: 232 IDPEPGQGEGQAPIGAFVTVESAVWKEVEISAEVLPEVNSSIDQVKQEIESGVLNLFKKI 291 Query: 301 AFTASYISQSVIGSLLLDTEGVVDYSGLKLNGVTGNVTLSETEVPLFSSV 350 AF + I S I +++ ++ V DY+ +K+NGV N+ LS E+P V Sbjct: 292 AFEDNVIRLSQINNIVYNSPSVSDYADIKINGVAENLVLSAVEIPKLGQV 341 >BS_xkdT [S] COG3299 Uncharacterized homolog of phage Mu protein gp47 Length = 348 Score = 253 bits (646), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 140/350 (40%), Positives = 205/350 (58%), Gaps = 11/350 (3%) Query: 3 VYEDQTFEALLGRMLDRVPSSLDKREGSIIYDXXXXXXXXXXQMYIELDVNSNLFFADTA 62 ++EDQTFEA++ RML+ + + +D REGS+IY+ + YI LD L F+DTA Sbjct: 1 MFEDQTFEAIMERMLNSISADIDTREGSVIYNALAPAAAELAKSYIWLDTVLELVFSDTA 60 Query: 63 TGEYLERSIAWSGITRRAASKAELKAMFYNSASGLLDVPLGSRFSLDLLHYTAVEKLAPG 122 GE+L+R A +GI R AA+KA F + + +P+GSRF +D L++ + A G Sbjct: 61 QGEFLDRRAAEAGIERTAATKAVRAGEFTSGVT----IPVGSRFYVDNLYF---QYTADG 113 Query: 123 TYRLESETAGEEGNRYFG--SLLPIDYIPVLARGEITALLIPGENAEDDEALRQRYLESV 180 T L ET GE GN +LL +D IP L + +LIPG E D++LR+RY V Sbjct: 114 T--LICETPGEAGNANLTGRNLLSLDTIPGLETAIVKEILIPGREEEGDDSLRERYFTRV 171 Query: 181 RRPSTSGNKYHYMEWALQIDGVGGARVFPLWNGPKTVKVVIVDAAKRPASELLVSQVQQY 240 RR + S NK HY EWA ++DGVG A++FPLWNG TVK+V+ +A PAS +L+ +V+ Y Sbjct: 172 RREAVSANKMHYKEWAEEVDGVGKAKIFPLWNGEGTVKIVVTNANLEPASPILIQKVKDY 231 Query: 241 IDPVSGQGEGQXXXXXXXXXXXXXGKSISVSAKVTLASGYSLQEVIDAFKEILEKYRKEK 300 IDP GQGEGQ K + +SA+V +S+ EV +E + + K+ Sbjct: 232 IDPEPGQGEGQAPIGAVVTVESAVWKEVEISAEVLPEINHSIDEVKSEIEEGVLNFFKKM 291 Query: 301 AFTASYISQSVIGSLLLDTEGVVDYSGLKLNGVTGNVTLSETEVPLFSSV 350 AF + I S I +++ ++ V DYS +++NG + N+ LS+ E+P V Sbjct: 292 AFEDNVIRLSQINNIVYNSPSVSDYSNIQINGTSENLVLSDVEIPKLGQV 341 >lin1287 [S] COG3299 Uncharacterized homolog of phage Mu protein gp47 Length = 361 Score = 198 bits (503), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 120/357 (33%), Positives = 189/357 (52%), Gaps = 6/357 (1%) Query: 4 YEDQTFEALLGRMLDRVPSSLDKREGSIIYDXXXXXXXXXXQMYIELDVNSNLFFADTAT 63 +E+ TFE ++ MLD VP++LDKREGS+I++ +Y L+ L F DTA Sbjct: 4 FEEMTFENIMESMLDAVPATLDKREGSMIWNSLAPAASQIALLYTWLENAFELVFYDTAP 63 Query: 64 GEYLERSIAWSGITRRAASKAELKAMFYNSASGLLDVPLGSRFSLDLLH-YTAVEKLAPG 122 E+L+R+ A G+ R+ A + + +++P GSRF ++ + Y V Sbjct: 64 DEFLDRAAAPYGVIRKPAVATQRIIKGIDENDNKIEIPEGSRFFVESENIYFKVTSSTIL 123 Query: 123 TYRLESETAGEEGNRYFG--SLLPIDYIPVLARGEITALLIPGENAEDDEALRQRYLESV 180 +E E AG +GN F ++L +D I LA+ EI +LIPG + E+ E R+R+ + Sbjct: 124 EQVVECEVAGTKGNADFSNDTILSLDNIYGLAKVEIGDILIPGSDEEELETFRKRFFTKI 183 Query: 181 RRPSTSGNKYHYMEWALQIDGVGGARVFPLWNGPKTVKVVIVDAAKRPASELLVSQVQQY 240 ++ + SGNK HY WA ++ GVG A+++PL G TVK+VI D PAS LV QV+ Y Sbjct: 184 QKDAFSGNKNHYKLWAEEVLGVGKAKIYPLGYGEGTVKIVITDTNLHPASPTLVEQVRNY 243 Query: 241 IDPVSGQGEGQXXXXXXXXXXXXXGKSISVSAKVTLASGYSLQEVIDAFKEILEKYRKEK 300 IDP+ +GEG K+I++S ++ L G S+ +VI+ + K K Sbjct: 244 IDPLPQKGEGVAPIGAKVFIESAAWKTINISGQLVLKDGKSVNDVIEEITMDINKLFKSI 303 Query: 301 AFTASYISQ---SVIGSLLLDTEGVVDYSGLKLNGVTGNVTLSETEVPLFSSVVLEV 354 F ++ +VI +++ V+D +KLN N+ L+E EVP + E+ Sbjct: 304 TFPEDELTSVKLAVISNIIFSAPSVLDNFNIKLNNEAANIILAENEVPQLGAFSFEL 360 >NMA1323 [S] COG3299 Uncharacterized homolog of phage Mu protein gp47 Length = 71 Score = 81.6 bits (200), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 0/283 (0%), Positives = 0/283 (0%), Gaps = 16/283 (5%) Query: 57 FFADTATGEYLERSIAWSGITRRAASKAELKAMFYNSASGLLDVPLGSRFSLDLLHYTAV 116 Sbjct: 58 117 Query: 117 EKLAPGTYRLESETAGEEG---NRYFGSLLPIDYIPVLARGEITALLIPGENAEDDEALR 173 Sbjct: 118 - 176 Query: 174 QRYLESVRRPSTSGNKYHYMEWALQIDGVGGARVFPLWNGPKTVKVVIVDAAKRPASELL 233 Sbjct: 177 - 235 Query: 234 VSQVQQYIDPVSGQGEGQXXXXXXXXXXXXXGKSISVSAKVTLASGYSLQEVIDAFKEIL 293 Sbjct: 236 ------- 288 Query: 294 EKYRKEKAFTASYISQSVIGSLLLDTEGVVDYSGLKLNGVTGN 336 Sbjct: 289 ---- 327 >NMA1826 [S] COG3299 Uncharacterized homolog of phage Mu protein gp47 Length = 133 Score = 67.4 bits (163), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 7/137 (5%), Positives = 24/137 (17%), Gaps = 6/137 (4%) Query: 160 LIPGENAEDDEALRQRYLESVRRPSTSGNKYHYMEWALQIDGVGGARVFPLWNGPKTVKV 219 + + E + ++S S + + L + + + K Sbjct: 1 MKSLFQFQFQEVTMKPKIDSTSFGSITVEGETFEYDILIRLDGRVEKREKMLSKGKYGTS 60 Query: 220 VIVDAAKRPASELLVSQVQQYIDPVSGQGEGQXXXXXXXXXXXXXGKSISVSAKVTLASG 279 + + + + + K Sbjct: 61 HKISLEEAEHIYEEGT-EKIIVGTGQTGFVELSEEAEDFFM-----KKKCGIELFPTPWA 114 Query: 280 YSLQEVIDAFKEILEKY 296 I+ + Sbjct: 115 IERWNEIEGRVSAMFHV 131 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.134 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,832,294 Number of extensions: 921422 Number of successful extensions: 1278 Number of sequences better than 1.0e-05: 5 Number of HSP's gapped: 1270 Number of HSP's successfully gapped: 5 Length of query: 354 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 247 Effective length of database: 26,037,693 Effective search space: 6431310171 Effective search space used: 6431310171 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 116 (49.3 bits)