BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0078.0035.p (220 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BMEII0448 [R] COG1451 Predicted metal-dependent hydrolase 104 8e-23 MA2993 [R] COG1451 Predicted metal-dependent hydrolase 96 3e-20 MA3501 [R] COG1451 Predicted metal-dependent hydrolase 95 7e-20 BMEI2015 [R] COG1451 Predicted metal-dependent hydrolase 81 2e-15 AGc2594 [G] COG1653 ABC-type sugar transport system, periplasmic... 77 3e-14 >BMEII0448 [R] COG1451 Predicted metal-dependent hydrolase Length = 246 Score = 104 bits (260), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 67/239 (28%), Positives = 111/239 (46%), Gaps = 28/239 (11%) Query: 6 IQLGDQTLQLHVQ-YGKQKKISLQIDMVGLIIVKAPTGASEEMIRSAVERHGEWILESXX 64 I G +T ++ ++K +++ + G+ ++ AP+ ++ + V + G W+L Sbjct: 9 IVFGGETFAFQIERTARRKTVAISVGFDGVRVL-APSDLDDDRVLGIVRKKGAWLLRKQA 67 Query: 65 XXXXXXXXXXXKEYQGEDKFLYLGKEYLLHELINTSEQDVXXXXXXXXXFY-------FA 117 KE+ + F YLG++Y L +LI V A Sbjct: 68 AYRELEGQPVAKEFVSGETFHYLGRQYRL-KLIPDETAVVTRIAARGSTLIAPVLPNGHA 126 Query: 118 SLKKIVAGRIPR--YQA---------------VLKVKPKSIEIVESRTKWGSCSADKKLT 160 S+++ R Y+A L + +++V+ +WGSC A ++ Sbjct: 127 SVQRAAVRNGLRHWYRAHAIQHFPIRARVIAKTLGIPTPIVKVVDQSKRWGSCDARGRIR 186 Query: 161 FNYRLAMAPMEVIDYVIIHELCHLLHMNHDRSFWRRLGSVMPDYREKGAFLARQG-QFM 218 N+RLAMAPM ++DYVI HE CH+L NH R FWR L ++MPDY ++ L R G QF+ Sbjct: 187 LNWRLAMAPMPLVDYVIAHEACHILEHNHSRRFWRSLEAIMPDYEDRLRRLDRMGHQFI 245 >MA2993 [R] COG1451 Predicted metal-dependent hydrolase Length = 130 Score = 96.3 bits (238), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 40/107 (37%), Positives = 64/107 (59%) Query: 114 FYFASLKKIVAGRIPRYQAVLKVKPKSIEIVESRTKWGSCSADKKLTFNYRLAMAPMEVI 173 FY +K++ R +Y KP I+I + KWG+CS +L FN+R+ MA M +I Sbjct: 24 FYHEKAEKVLKQRARKYLLYFDDKPSLIKIQTLKNKWGNCSKTNQLRFNWRVIMAKMSII 83 Query: 174 DYVIIHELCHLLHMNHDRSFWRRLGSVMPDYREKGAFLARQGQFMTL 220 DYV++HELCH + +H ++FW + ++PDY E+ A+L G + + Sbjct: 84 DYVVVHELCHTKYKDHSKAFWNEVQKILPDYEERKAWLRVHGDLLKI 130 >MA3501 [R] COG1451 Predicted metal-dependent hydrolase Length = 245 Score = 95.1 bits (235), Expect = 7e-20, Method: Compositional matrix adjust. Identities = 63/234 (26%), Positives = 103/234 (44%), Gaps = 24/234 (10%) Query: 6 IQLGDQTLQLHVQYGKQKKISLQIDMVGLII-VKAPTGASEEMIRSAVERHGEWILESXX 64 I+ +T+ + Y K+++ + + L + +AP G S E IR +++ W+ E Sbjct: 7 IKACGRTIDYEIVYSKRRRKAAIVVRPDLKVEFRAPHGLSLEAIRDMLQKKAGWVQEKLD 66 Query: 65 XXXXXXXXXXXKEYQGEDKFLYLGKEYLLHEL-----------INTSEQDVXXXXXXXXX 113 K Y + +LY+G+EY L L N +E V Sbjct: 67 WFEANRLPGQEKRYTEGEIYLYMGREYPLKILAGKGIKKPLASFNGAELTVLIPEAVPEE 126 Query: 114 ------------FYFASLKKIVAGRIPRYQAVLKVKPKSIEIVESRTKWGSCSADKKLTF 161 FY + V + Y LK+ P ++ + +WGSCSAD + Sbjct: 127 LRPVLVKKAVWDFYRECAESNVERLLKIYSEKLKIAPPIFKVKYQKRRWGSCSADNVIRI 186 Query: 162 NYRLAMAPMEVIDYVIIHELCHLLHMNHDRSFWRRLGSVMPDYREKGAFLARQG 215 N++L MAP E ++YV++HELCH+ NH FW+ +G +MP Y L ++G Sbjct: 187 NFQLMMAPPEQLEYVVVHELCHVKEKNHSFRFWKLVGGLMPGYEIHRKSLKKEG 240 >BMEI2015 [R] COG1451 Predicted metal-dependent hydrolase Length = 253 Score = 80.9 bits (198), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 32/90 (35%), Positives = 55/90 (61%) Query: 129 RYQAVLKVKPKSIEIVESRTKWGSCSADKKLTFNYRLAMAPMEVIDYVIIHELCHLLHMN 188 R+ A + K K++ +++++WGSC++D L+F++R+ MAP VI+Y++ HE HL+ MN Sbjct: 158 RHTAAVGRKAKAVRFKDTKSRWGSCTSDGVLSFSWRIGMAPPPVINYLVAHEAAHLIEMN 217 Query: 189 HDRSFWRRLGSVMPDYREKGAFLARQGQFM 218 H FW+ + PD A+L G + Sbjct: 218 HGPKFWKLCQELCPDTERCKAWLKHNGSAL 247 >AGc2594 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 218 Score = 76.6 bits (187), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 0/78 (0%), Positives = 0/78 (0%) Query: 139 KSIEIVESRTKWGSCSADKKLTFNYRLAMAPMEVIDYVIIHELCHLLHMNHDRSFWRRLG 198 Sbjct: 170 229 Query: 199 SVMPDYREKGAFLARQGQ 216 Sbjct: 230 247 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.137 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 13,461,856 Number of extensions: 478102 Number of successful extensions: 691 Number of sequences better than 1.0e-05: 44 Number of HSP's gapped: 690 Number of HSP's successfully gapped: 44 Length of query: 220 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 118 Effective length of database: 26,614,363 Effective search space: 3140494834 Effective search space used: 3140494834 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 114 (48.5 bits)