BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0078.0033.p (642 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mll5256 [E] COG0367 Asparagine synthase (glutamine-hydrolyzing) 200 5e-51 BS_asnO [E] COG0367 Asparagine synthase (glutamine-hydrolyzing) 85 3e-16 CAC2243 [E] COG0367 Asparagine synthase (glutamine-hydrolyzing) 75 3e-13 PA2084 [E] COG0367 Asparagine synthase (glutamine-hydrolyzing) 72 3e-12 L0095 [E] COG0367 Asparagine synthase (glutamine-hydrolyzing) 71 5e-12 >mll5256 [E] COG0367 Asparagine synthase (glutamine-hydrolyzing) Length = 252 Score = 200 bits (509), Expect = 5e-51, Method: Compositional matrix adjust. Identities = 0/652 (0%), Positives = 0/652 (0%), Gaps = 81/652 (12%) Query: 1 MSAIAGIYHLQHQPLDPKQGPDLMQQLSRY---PADEIRTWQSTDVFLGCLSQWVTPQSL 57 MS +L + + + Sbjct: 1 MSDWNPSLYLHFSAERSRP--AVELLARVPLENVEYVADLGCGPGNSTA--LLQQRWPAA 56 Query: 58 GETLPYYDSMRKLAITADAIIDNREELFERLQVPRGDRVSMPDSLLILLAYEKWGEQAPV 117 T + E R P + ++ Sbjct: 57 RITGIDSSPAMIAEARSALPDCQFVEADIRNWQP--VQ-ALDLIFANASLQWLPDHYELF 113 Query: 118 YLVGDFAFMIWDERKRALFGARDFSGNRTLYFLRSGGLLSFCTVIHPLLSMPGADRTLNE 177 + + + + + + Sbjct: 114 PHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAG 173 Query: 178 RWASEFLAIHTRTDVSDQYSTVYQSIEQLPPSHSISVSGGNILFSRYCTLQAPPELR-LR 236 + T S + + + Sbjct: 174 CEVDIWRT----TYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKR--YHQMLEE 227 Query: 237 SDQEYEEAFREVFGQAVRDRLRTFRAVGANXXXXXXXXXXXXFAAGELRRQGQP-LHTFS 295 Sbjct: 228 QYP-- 285 Query: 296 SYPVDNFVDFNIGKRIADERPYIQETIDYVGNIQPNFLNFPDLSPYSEIDEWLDIMEMPY 355 Sbjct: 286 ----------- 334 Query: 356 KFIENSYWL-KGIFEQAQMKNIGVLLSGQRGNWTISWGPALDYQAKLIREFRWISFYREN 414 Sbjct: 335 -- 392 Query: 415 RKYNESMGANRRKVFQIVGKKAFPSLTGLLPAKHEAPP---------------------- 452 Sbjct: 393 ------- 445 Query: 453 --DLIHPDFARRTDVDERLKALGINSGSSQTIYDIRRKHFEQ----PHVWNVNGTIATKL 506 Sbjct: 446 505 Query: 507 SL--KYRVWDRDPTNDLRVIRFCLSVPESQYVQQGVDRSLIRRAMKGLLPDEVRLNRKRR 564 Sbjct: 506 ------ 559 Query: 565 GVQGADGIYRMLPKWDAFIQELREMIQDP-RTSAYLNNTGLAGCLDRLGSEP 615 Sbjct: 560 --- 608 >BS_asnO [E] COG0367 Asparagine synthase (glutamine-hydrolyzing) Length = 252 Score = 85.1 bits (209), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 0/489 (0%), Positives = 0/489 (0%), Gaps = 47/489 (9%) Query: 80 NREELFERLQVPRGDRVSMPDSLLILLAYEKWGEQAPVYLVGDFAFMIWDERKRALFGAR 139 + + + + + + + + + D + Sbjct: 59 TGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVS 118 Query: 140 DFSGNRTLYFLRSGGLLSFCTVIHPLLSMPGADRTLNERWASEFLAIH-TRTDVSDQYST 198 + L L V+ ++ + A + + + Sbjct: 119 LLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILS----EAGC 174 Query: 199 VYQSIEQLPPSHSISVSGGNILFSRYCTLQAPPELRLRSDQEYEEAFREVFGQAVRDRLR 258 S + L ++ R + L+ Sbjct: 175 EVDIWRTTYYHQMPSHQAIIDWVTATGLRPW--LQDLTESEQQLFLKRYHQMLEEQYPLQ 232 Query: 259 TFRAVGANXXXXXXXXXXXXFAAGELRRQGQ-PLHTFSSYPVDNFVDFNIGK-RIADERP 316 Sbjct: 233 292 Query: 317 YIQETIDYVGNIQPN-FLNFPDLSPYSEIDEWLDIMEMPYKFIENSYWLKGIFEQAQMKN 375 Sbjct: 293 ---- 348 Query: 376 IGVLLSGQRGNWTISWGPALDYQAKLIREFRWISFYRENRKYNESMGANRRKVFQIVGKK 435 Sbjct: 349 - 407 Query: 436 AFPSLTGLLPAKHEAPPDLIHPDFARRTDVDERLKALGINSGSSQTIYDIRRKHFEQPHV 495 Sbjct: 408 ------------------------------ 437 Query: 496 WNVNGTIATK--LSLKYRVWDRDPTNDLRVIRFCLSVPESQYVQQGVDRSLIRRAMKGLL 553 Sbjct: 438 497 Query: 554 PDEVRLNRK 562 Sbjct: 498 506 >CAC2243 [E] COG0367 Asparagine synthase (glutamine-hydrolyzing) Length = 252 Score = 75.1 bits (183), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 0/396 (0%), Positives = 0/396 (0%), Gaps = 17/396 (4%) Query: 1 MSAIAGIYHLQHQPLDPKQG-PDLMQQLSRYPADEIRTWQSTDVFLGCLSQWVT-PQSLG 58 MS +L + L + L Q + G Sbjct: 1 MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITG 60 Query: 59 ETLPYYDSMRKLAITADAIIDNREELFERLQVPRGDRVSMPDSLLILLAYEKWGEQAPVY 118 + D + + + + YE + + Sbjct: 61 IDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLL 120 Query: 119 LVGDFAFMIWDERKRALFGARDFSGNRTLYFLRSGGLLSFCTVIHPLLSMPGADRTLNER 178 + + + G + + R Sbjct: 121 NPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWR 180 Query: 179 WASEFLAIHTRTDVSDQYSTVYQSIEQLPPSHSISVSGGNILFSRYCTLQAPPELRLRSD 238 + + + + S + + P Sbjct: 181 TTYYHQMPSHQAIID---WVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQ- 236 Query: 239 QEYEEAFREVFGQAVRDRLRTFRAVGANXXXXXXXXXXXXFAAGELRRQGQPLHTFSSYP 298 Sbjct: 237 - 295 Query: 299 VDNFVDF-NIGKRIADERPYIQETIDYVGNIQPNFLNFPDLSPYSEIDEWLDIMEMP-YK 356 Sbjct: 296 - 354 Query: 357 FIENSYWL--KGIFEQAQMKNIGVLLSGQRGNWTIS 390 Sbjct: 355 ----- 385 >PA2084 [E] COG0367 Asparagine synthase (glutamine-hydrolyzing) Length = 252 Score = 72.0 bits (175), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 0/500 (0%), Positives = 0/500 (0%), Gaps = 41/500 (8%) Query: 69 KLAITADAIIDNREELFERLQVPRGDRVSMPDSLLILLAYEKWGEQAPVYLVGDFAFMIW 128 + ++ V D + SL L + + L + Sbjct: 70 EARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQ 129 Query: 129 DERKRALFGARDFSGNRTLYFLRSGGLLSFCTVIHPLLSMPGADRTLNERWASEFLAIHT 188 G V + A ++ + + + Sbjct: 130 MPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQM-- 187 Query: 189 RTDVSDQYSTVYQSIEQLPPSHSISVSGGNILFSRYCTLQAPPELRLRSDQEYEEAFREV 248 V + + L + L+ + L+ + + AF + Sbjct: 188 -PSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEE--QYPLQENGQILLAFPRL 244 Query: 249 FGQAVRDRLRTFRAVGANXXXXXXXXXXXXFAAGELRRQGQPLHTFS-SYPVDNFVDFNI 307 Sbjct: 245 304 Query: 308 GKRIADERPYIQETIDYVGNIQPNFLNFPDLSPYSEIDEWLDIMEMP-YKFIENSYWLKG 366 Sbjct: 305 --- 361 Query: 367 IFEQAQMKNIGVLLSGQRGNWTISWGPALDYQAKLIRE-FRWISFYRENRKYNESMGANR 425 Sbjct: 362 --------------- 406 Query: 426 RKVFQIVGKKAFPSLTGLLPAKHEAPPDLIHPDFARRTDVDERLKALGINSGSSQTIYDI 485 Sbjct: 407 --------- 457 Query: 486 RRKHFEQPHVWNVNGTIATKLSLKYRVWDRDPTNDLRVIRFCLSVPESQYVQQGVDRSLI 545 Sbjct: 458 ----- 512 Query: 546 RRAMKGLLPDEVRLNRKRRG 565 Sbjct: 513 - 531 >L0095 [E] COG0367 Asparagine synthase (glutamine-hydrolyzing) Length = 252 Score = 71.2 bits (173), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 5/164 (3%), Positives = 23/164 (14%), Gaps = 12/164 (7%) Query: 58 GETLPYYDSMRKLAITADAIIDNREELFERLQVPRGDRVSMPDSLLILLAYEKWGEQAPV 117 T + E R P + + + + + + Sbjct: 57 RITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHL 116 Query: 118 YLVGDFAFMIWDERKRALFGARDFSGNRTLYFLRSGGLLSFCTVIHPLLSMPGADRTLNE 177 + + ++ + + ++ Sbjct: 117 VSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEV 176 Query: 178 RWASEFLAIHTRTDVSDQYS----TVYQSIEQLPPSHSISVSGG 217 Q + ++ Sbjct: 177 DIWRTTYYH--------QMPSHQAIIDWVTATGLRPWLQDLTES 212 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.139 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 48,226,333 Number of extensions: 2057049 Number of successful extensions: 4557 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 4553 Number of HSP's successfully gapped: 13 Length of query: 642 Length of database: 38,378,431 Length adjustment: 113 Effective length of query: 529 Effective length of database: 25,345,689 Effective search space: 13407869481 Effective search space used: 13407869481 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 119 (50.4 bits)