BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0078.0024.p (411 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA1161 [GEPR] COG0477 Permeases of the major facilitator superfa... 99 1e-20 NMA1161 [P] COG1629 Outer membrane receptor proteins, mostly Fe ... 99 1e-20 TM0342 [GEPR] COG0477 Permeases of the major facilitator superfa... 86 2e-16 all2673 [GEPR] COG0477 Permeases of the major facilitator superf... 74 3e-13 PAB0724 [GEPR] COG0477 Permeases of the major facilitator superf... 72 2e-12 >MA1161 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 443 Score = 99.0 bits (245), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 85/359 (23%), Positives = 154/359 (42%), Gaps = 15/359 (4%) Query: 16 QTVSLFGSSLVQYAILWYITLNTQSGLMMTISIICGFVPTFLLSPFAGVWADRYSRKLLI 75 Q +S+ GS L +++ Y+ T + ++C F+P FLL P+ G+ ADR+ R+L++ Sbjct: 19 QFISIIGSGLTTFSLGIYVYQQTGTASSYVFILMCAFLPPFLLKPYGGILADRHDRRLMM 78 Query: 76 ILSDSCIXXXXXXXXXXXXMGYDEMWLLFVVSAVRALGTAVQTPAVGAILPQLVPEEHLT 135 IL D G E+W +++ A+ ++ +A Q PA A++ L+PE Sbjct: 79 ILGDLGSTLGLLFIFAMMLKGNIELWQIYLGIAISSVFSAFQEPAYKALITDLLPENQYA 138 Query: 136 KVNGTNGSIQALIMLLSPMLSGALLTVASIEVIFFIDVITXXXXXXXXXXFVKVPLHAKA 195 K +G + L+SP L+G +LT+ I+ +F IDV T K+ L Sbjct: 139 KASGLMQLASSAQYLISPFLAGIILTIMDIKFVFLIDVTTFLIASSIVIWIRKI-LSRTQ 197 Query: 196 AGEQTLSYFNDMRLGF-SYIKQHNYIRXXXXXXXXXXXXXXXXXXXXXXQVTRTFGDDIW 254 +Q + D++ G + K + + T + Sbjct: 198 ITKQEQNNMADLKEGIQEFSKNRGIVNLVITTTLILFFVGLLQSLFIPMMLNLTTVKAVG 257 Query: 255 RLTAIEIAFSXXXXXXXXXXAGWQGFRNR-VYTMTMSSLAIGVFTFALGVIPVFWIYLLF 313 + +I G G +N+ V T+++S G+F LG+ ++F Sbjct: 258 TIQSI----CASGILVGSLFIGVYGSKNKYVKTLSISLFVSGIFFANLGLST----NVIF 309 Query: 314 MGLVGL----AMPFFNTPSTVLLQEKVEPDYLGRVFGVLGMISSSMMPLGMLIFGPLAD 368 + L G +PF NT VL+++ +E GR++ ++ M++ + + G LAD Sbjct: 310 ITLAGFMFFSTLPFINTAIEVLIRKNIENSKQGRIWSIISMVTYLGSIIAFAVAGFLAD 368 >NMA1161 [P] COG1629 Outer membrane receptor proteins, mostly Fe transport Length = 443 Score = 99.0 bits (245), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 85/359 (23%), Positives = 154/359 (42%), Gaps = 15/359 (4%) Query: 16 QTVSLFGSSLVQYAILWYITLNTQSGLMMTISIICGFVPTFLLSPFAGVWADRYSRKLLI 75 Q +S+ GS L +++ Y+ T + ++C F+P FLL P+ G+ ADR+ R+L++ Sbjct: 19 QFISIIGSGLTTFSLGIYVYQQTGTASSYVFILMCAFLPPFLLKPYGGILADRHDRRLMM 78 Query: 76 ILSDSCIXXXXXXXXXXXXMGYDEMWLLFVVSAVRALGTAVQTPAVGAILPQLVPEEHLT 135 IL D G E+W +++ A+ ++ +A Q PA A++ L+PE Sbjct: 79 ILGDLGSTLGLLFIFAMMLKGNIELWQIYLGIAISSVFSAFQEPAYKALITDLLPENQYA 138 Query: 136 KVNGTNGSIQALIMLLSPMLSGALLTVASIEVIFFIDVITXXXXXXXXXXFVKVPLHAKA 195 K +G + L+SP L+G +LT+ I+ +F IDV T K+ L Sbjct: 139 KASGLMQLASSAQYLISPFLAGIILTIMDIKFVFLIDVTTFLIASSIVIWIRKI-LSRTQ 197 Query: 196 AGEQTLSYFNDMRLGF-SYIKQHNYIRXXXXXXXXXXXXXXXXXXXXXXQVTRTFGDDIW 254 +Q + D++ G + K + + T + Sbjct: 198 ITKQEQNNMADLKEGIQEFSKNRGIVNLVITTTLILFFVGLLQSLFIPMMLNLTTVKAVG 257 Query: 255 RLTAIEIAFSXXXXXXXXXXAGWQGFRNR-VYTMTMSSLAIGVFTFALGVIPVFWIYLLF 313 + +I G G +N+ V T+++S G+F LG+ ++F Sbjct: 258 TIQSI----CASGILVGSLFIGVYGSKNKYVKTLSISLFVSGIFFANLGLST----NVIF 309 Query: 314 MGLVGL----AMPFFNTPSTVLLQEKVEPDYLGRVFGVLGMISSSMMPLGMLIFGPLAD 368 + L G +PF NT VL+++ +E GR++ ++ M++ + + G LAD Sbjct: 310 ITLAGFMFFSTLPFINTAIEVLIRKNIENSKQGRIWSIISMVTYLGSIIAFAVAGFLAD 368 >TM0342 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 413 Score = 85.5 bits (210), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 77/396 (19%), Positives = 151/396 (38%), Gaps = 20/396 (5%) Query: 4 NWKKNIVLFLTSQTVSLFGSSLVQYAILWYITLNTQSGLMMTISIICGFVPTFLLSPFAG 63 +WK+N L + VS+ GSS+ AI ++ T SG + + + VP L +P AG Sbjct: 6 HWKRNFFLLALGRFVSILGSSVQAVAIPLFVLDLTHSGSAVALMTVAYMVPRILFTPIAG 65 Query: 64 VWADRYSRKLLIILSDSCIXXXXXXXXXXXXMGYDEMWLLFVVSAVRALGTAVQTPAVGA 123 + DR RK L++ D + +L+V A+ + GA Sbjct: 66 IVGDRGDRKFLMVSMDFLRGFLICFLALLAFRNSITLPVLYVSQIAMAIMDNLFDIPTGA 125 Query: 124 ILPQLVPEEHLTKVNGTNGSIQALIMLLSPMLSGALLTVASIEVIFFIDVITXXXXXXXX 183 + P +VP++ L + N G ++ +L P L G + I ++F ++ ++ Sbjct: 126 MFPDIVPQDFLMRANSIMGMVRIFPQILGPALGGVIYGFYGIAIVFLVNGVS-FVLSAIS 184 Query: 184 XXFVKVPLHAKAAGEQTLSYFNDMRLGFSYIKQHNYIRXXXXXXXXXXXXXXXXXXXXXX 243 F+ + + D++ GF +I H IR Sbjct: 185 EIFIVYSRKVEKKPAKFSQILEDLKEGFLFIWNHELIRTILIFALFLNLLISPLFMVVYP 244 Query: 244 QVTRTFGDDIWRLTAIEIAF------------SXXXXXXXXXXAGWQGFRNRVYTMTMSS 291 R ++ + +A E F + W+ + ++ + Sbjct: 245 YTIR----EVMKFSAQEFGFLETSFTLGALLGNFLIASYLSKKNMWKAMKISIFLFELPI 300 Query: 292 LAIGVFT---FALGVIPVFWIYLLFMGLVGLAMPFFNTPSTVLLQEKVEPDYLGRVFGVL 348 + +G F+L + +F ++ + ++G P N P Q+ R F L Sbjct: 301 VFLGFLVMPDFSLNRLVLFSLFFITFLIMGFLNPIINVPLDTAFQKMTPSHIRARAFSFL 360 Query: 349 GMISSSMMPLGMLIFGPLADVLKIEWMLMLTGLLMF 384 ++++ + PLG +++G L D + + ++ + G L F Sbjct: 361 ILVANGVTPLGSVLYGYLVDRVPVHFLYYVVGALSF 396 >all2673 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 437 Score = 74.3 bits (181), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 73/355 (20%), Positives = 138/355 (38%), Gaps = 3/355 (0%) Query: 16 QTVSLFGSSLVQYAILWYITLNTQSGLMMTISIICGFVPTFLLSPFAGVWADRYSRKLLI 75 Q +S GS + +A+ ++ T S + + +P L++ FAG+ DR++R+ L+ Sbjct: 11 QIISTIGSFMTVFALTIWVWDKTGSATALALVGFFAQMPKILITTFAGIAVDRFNRRFLM 70 Query: 76 ILSDSCIXXXXXXXXXXXXMGYDEMWLLFVVSAVRALGTAVQTPAVGAILPQLVPEEHLT 135 IL++ ++W L+++ + +Q A A + LVP+E T Sbjct: 71 ILAEVVAVLCTLAIAILYLNQQLQVWHLYIIVMLYGGFGQLQVLAYSASISLLVPKEQFT 130 Query: 136 KVNGTNGSIQALIMLLSPMLSGALLTVASIEVIFFIDVITXXXXXXXXXXFVKVPLHAKA 195 + ++ + SP L+G + ++ IF+ID +T VK+P A Sbjct: 131 RAESLGSAVNYSAAIFSPALAGFIYPRLGLQAIFWID-LTTFAAAFITLLIVKIPQPAVE 189 Query: 196 AG--EQTLSYFNDMRLGFSYIKQHNYIRXXXXXXXXXXXXXXXXXXXXXXQVTRTFGDDI 253 + T S + + GF YI + + + G + Sbjct: 190 PNHRDNTESLWQKLTFGFRYIWANPSLTAMAIAFTLFAIPNDISKVLYTPMILARTGGNS 249 Query: 254 WRLTAIEIAFSXXXXXXXXXXAGWQGFRNRVYTMTMSSLAIGVFTFALGVIPVFWIYLLF 313 L + A + W GF R++ M + + G+F +G+ +++ Sbjct: 250 EILGTVTTAAGIGGVIGAIALSIWGGFHRRIHGMLLGFIGHGIFRTLIGLGQTVSVWMGA 309 Query: 314 MGLVGLAMPFFNTPSTVLLQEKVEPDYLGRVFGVLGMISSSMMPLGMLIFGPLAD 368 L +P F + S + + P GRV +I ++ + LI G LAD Sbjct: 310 HFLSSTLIPLFYSSSNAIWYATIPPGLQGRVLAADQLIGVTVAAITPLIAGFLAD 364 >PAB0724 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 418 Score = 71.6 bits (174), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 79/379 (20%), Positives = 147/379 (38%), Gaps = 23/379 (6%) Query: 6 KKNIVLFLTSQTVSLFGSSLVQYAILWYITLNTQSGLMMTISIICGFVPTFLLSPFAGVW 65 +N LF+ + +S G ++ + A+ Y+ T S +MT+ ++ +P+ + +P AGV+ Sbjct: 2 NRNFWLFVAGRFISQLGWAIQEVALPLYVLDVTHSAKIMTLFVLADLIPSIITTPIAGVF 61 Query: 66 ADRYSRKLLIILSDSCIXXXXXXXXXXXXMGYDEMWLLFVVSAVRALGTAVQTPAVGAIL 125 DRY RK +++ D + + L VV + A+ A + GA+ Sbjct: 62 GDRYDRKKIMVGFD---LIRGVLLFLVIAFNFLTIRCLLVVQVIMAIMNAFFSAGTGAMY 118 Query: 126 PQLVPEEHLTKVNGTNGSIQALIMLLSPMLSGALLTVASIEVIFFIDVITXXXXXXXXXX 185 P LV E L + N S + L+ P + G + I++ ++ I+ Sbjct: 119 PDLVKPEELERANSIVMSASIISRLVGPAIGGFVYGFGGIKLAILMNAIS-FFGSGLFEI 177 Query: 186 FVKVPLHAKAAGEQTLSYFNDMRLGFSYIKQHNYIRXXXXXXXXXXXXXXXXXXXXXXQV 245 F+K + E + D++ G +I +++I Sbjct: 178 FIKYEWKTRNI-EGFSDFVRDIKEGLKFIMSNSFISTLIGLVILLNALGAPFASIIYPYA 236 Query: 246 TRTFGDDIWRLTA-----IEIAFSXXXXXXXXXXAGWQGFRNRVYTMTMSSLAIGV-FTF 299 R +I + ++ +E AF + G + Y + + V F Sbjct: 237 MR----EILKFSSQQFGLVESAFMLGALIGNLIVGAFLGAKAGRYIFRLFLVNGAVELIF 292 Query: 300 ALGVIP--------VFWIYLLFMGLVGLAMPFFNTPSTVLLQEKVEPDYLGRVFGVLGMI 351 L + P F + L+ +G+A N P LQ V + GRVF + M+ Sbjct: 293 ILIISPFLALPRNSAFMLLLVLGATMGMAGAMINVPLASKLQRAVPSEIRGRVFSTMEML 352 Query: 352 SSSMMPLGMLIFGPLADVL 370 + PL +++ GPL D + Sbjct: 353 ARIANPLSLVVVGPLVDTI 371 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.141 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,198,152 Number of extensions: 903931 Number of successful extensions: 3749 Number of sequences better than 1.0e-05: 20 Number of HSP's gapped: 3748 Number of HSP's successfully gapped: 20 Length of query: 411 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 302 Effective length of database: 25,807,025 Effective search space: 7793721550 Effective search space used: 7793721550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 117 (49.7 bits)