BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0078.0019.p (543 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ytcI [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid... 635 0.0 lin2868 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid... 628 e-180 SA2402 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ... 621 e-178 BH3201 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ... 616 e-176 Ta1508m [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid... 326 7e-89 >BS_ytcI [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases Length = 346 Score = 635 bits (1637), Expect = 0.0, Method: Compositional matrix adjust. Identities = 0/528 (0%), Positives = 0/528 (0%), Gaps = 9/528 (1%) Query: 14 LDNQQWIAPEFYNLTSEMEHHPA--DRIALKWLQENGNYEEITYGDLMKQANRLAGGLSG 71 M + A L G Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 72 LGLTQGDRVLVMVPRRIIAYVIYLACLKLGLAVIPSSEMLRAKDLAYRLRHSEARAVIVW 131 L T L R + R ++ + Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQT 123 Query: 132 SEVTGEVNKITGDLPALDYRLAVGGKEEELGSGWAGLEGLMEGQPESFPAVASRRDDIAI 191 + +G + A L ++ I Sbjct: 124 DYLDLYQVHWPQRPTNCF--GKLG----YSWTDSAPAVSL-LDTLDALAEYQRAGKIRYI 176 Query: 192 LAYTSGTTGNPKAVVHTHGWGYAHLKIVSSLWLDIRESDTVWATAAPGWQKWIWSPFLTV 251 G + + + + + + + S V ++ + + Sbjct: 177 GVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCL 236 Query: 252 LGNGATGFVYNGPFHPDRFLQLLQDQGIQVLCCTPTEYRLMAKTEGLARYDLSKLRCAVS 311 TG NG L T D +++ A Sbjct: 237 GFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFV 296 Query: 312 AGEPLNQEVINTFREHFDITIRDGYGQTESTLIIAALKDQPIRVGSMGKSIAPGIVEVID 371 Sbjct: 297 356 Query: 372 GEGNPLPAGVVGDIAVHLSMPALFKNYYKDPERKVNCSHGEYFVTGDRARKDEDGYFWFE 431 Sbjct: 357 416 Query: 432 GRGDDIIISSGYTIGPFEVEEALMKHALVKECAVVASPDEIRGSVVKAFVVLKDHSAGTP 491 Sbjct: 417 476 Query: 492 ELVKELQSHVKEWTAPYKYPRKIEFIADLPKTASGKIRRVELRDQEKK 539 Sbjct: 477 524 >lin2868 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases Length = 346 Score = 628 bits (1620), Expect = e-180, Method: Compositional matrix adjust. Identities = 0/524 (0%), Positives = 0/524 (0%), Gaps = 5/524 (0%) Query: 14 LDNQQWIAPEFYNLTSEMEHHPADRIALKWLQENGNYEEITYGDLMKQANRLAGGLSGLG 73 + + T + + + G + + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 74 LTQGDRVLVMVPRRIIAYVIYLACLKLGLAVIPSSEMLRAKDLAYRLRHSEARAVIVWSE 133 V L +++ D A ++ Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 134 VTGEVNKITGDLPALDYRLAVGGKEEELGSGWAGLEGLMEGQPESFPAVASRRDDIAILA 193 + + + + GK ++ A+A + I Sbjct: 121 LQTDYLDLY-QVHWPQRPTNCFGKL----GYSWTDSAPAVSLLDTLDALAEYQRAGKIRY 175 Query: 194 YTSGTTGNPKAVVHTHGWGYAHLKIVSSLWLDIRESDTVWATAAPGWQKWIWSPFLTVLG 253 + + H L + ++ + + ++ L Sbjct: 176 IGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSC 235 Query: 254 NGATGFVYNGPFHPDRFLQLLQDQGIQVLCCTPTEYRLMAKTEGLARYDLSKLRCAVSAG 313 G + +A +AR A Sbjct: 236 LGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAF 295 Query: 314 EPLNQEVINTFREHFDITIRDGYGQTESTLIIAALKDQPIRVGSMGKSIAPGIVEVIDGE 373 Sbjct: 296 355 Query: 374 GNPLPAGVVGDIAVHLSMPALFKNYYKDPERKVNCSHGEYFVTGDRARKDEDGYFWFEGR 433 Sbjct: 356 415 Query: 434 GDDIIISSGYTIGPFEVEEALMKHALVKECAVVASPDEIRGSVVKAFVVLKDHSAGTPEL 493 Sbjct: 416 475 Query: 494 VKELQSHVKEWTAPYKYPRKIEFIADLPKTASGKIRRVELRDQE 537 Sbjct: 476 519 >SA2402 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases Length = 346 Score = 621 bits (1602), Expect = e-178, Method: Compositional matrix adjust. Identities = 0/526 (0%), Positives = 0/526 (0%), Gaps = 8/526 (1%) Query: 20 IAPEFYNLTSEMEH--HPADRIALKWLQENGNYEEITYGDLMKQANRLAGGLSGLGLTQG 77 +T ++ A + + N ++ + G Sbjct: 14 STLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWL 73 Query: 78 DRVLVMVPRRIIAYVIYLACLKLGLAVIPSSEMLRAKDLAYRLRHSEARAVIVWSEVTGE 137 + I + V + + + A + + Sbjct: 74 AKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHW 133 Query: 138 VNKITGDLPALDYRLAVGGKEEELGSGWAGLEGLMEGQPESFPAVASRRDDIAILAYTSG 197 + T + + L+ L A+ Sbjct: 134 PQRPT-NCFGKLGYSWTDSAP-----AVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVM 187 Query: 198 TTGNPKAVVHTHGWGYAHLKIVSSLWLDIRESDTVWATAAPGWQKWIWSPFLTVLGNGAT 257 + V + F T+ G Sbjct: 188 RYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLN 247 Query: 258 GFVYNGPFHPDRFLQLLQDQGIQVLCCTPTEYRLMAKTEGLARYDLSKLRCAVSAGEPLN 317 G G + A+ L+ +R L Sbjct: 248 GAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLL 307 Query: 318 QEVINTFREHFDITIRDGYGQTESTLIIAALKDQPIRVGSMGKSIAPGIVEVIDGEGNPL 377 Sbjct: 308 367 Query: 378 PAGVVGDIAVHLSMPALFKNYYKDPERKVNCSHGEYFVTGDRARKDEDGYFWFEGRGDDI 437 Sbjct: 368 427 Query: 438 IISSGYTIGPFEVEEALMKHALVKECAVVASPDEIRGSVVKAFVVLKDHSAGTPELVKEL 497 Sbjct: 428 487 Query: 498 QSHVKEWTAPYKYPRKIEFIADLPKTASGKIRRVELRDQEKKAAQE 543 Sbjct: 488 533 >BH3201 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases Length = 346 Score = 616 bits (1589), Expect = e-176, Method: Compositional matrix adjust. Identities = 0/526 (0%), Positives = 0/526 (0%), Gaps = 7/526 (1%) Query: 20 IAPEFYNLTSEMEHHPAD--RIALKWLQENGNYEEITYGDLMKQANRLAGGLSGLGLTQG 77 I +++ + + +Y +L+ A Sbjct: 6 IPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLT 65 Query: 78 DRVLVMVPRRIIAYVIYLACLKLGLAVIPSSEMLRAKDLAYRLRHSEARAVIVWSEVTGE 137 + + + + + K+ + + +R R EA + T Sbjct: 66 ETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDY 125 Query: 138 VNKITGDLPALDYRLAVGGKEEELGSGWAGLEGLMEGQPESFPAVASRRDDIAILAYTSG 197 ++ P S + A R I + ++ Sbjct: 126 LDLYQVHWPQRPTNCFGKLGY----SWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNE 181 Query: 198 TTGNPKAVVHTHGWGYAHLKIVSSLWLDIRESDTVWATAAPGWQKWIWSPFLTVLGNGAT 257 T +H + + A + + + LG G Sbjct: 182 TAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTL 241 Query: 258 GFVYNGPFHPDRFLQLLQDQGIQVLCCTPTEYRLMAKTEGLARYDLSKLRCAVSAGEPLN 317 Y P L + + + Sbjct: 242 TGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPF 301 Query: 318 QEVINTFREHFDITIRDGYGQTESTLIIAALKDQPIRVGSMGKSIAPGIVEVIDGEGNPL 377 Sbjct: 302 361 Query: 378 PAGVVGDIAVHLSMPALFKNYYKDPERKVNCSHGEYFVTGDRARKDEDGYFWFEGRGDDI 437 Sbjct: 362 421 Query: 438 IISSGYTIGPFEVEEALMKHALVKECAVVASPDEIRGSVVKAFVVLKDHSAGTPE-LVKE 496 Sbjct: 422 481 Query: 497 LQSHVKEWTAPYKYPRKIEFIADLPKTASGKIRRVELRDQEKKAAQ 542 Sbjct: 482 527 >Ta1508m [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases Length = 211 Score = 326 bits (835), Expect = 7e-89, Method: Compositional matrix adjust. Identities = 0/492 (0%), Positives = 0/492 (0%), Gaps = 22/492 (4%) Query: 49 NYEEITYGDLMKQANRLAGGLSGLGLTQGDRVLVMVPRRIIAYVIYLACLKLGLAVIPSS 108 + E+ L+ + + +GL + Sbjct: 28 SPFELLIAVLLSAQATDVSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAE 87 Query: 109 EMLRAKDLAYRLRHSEARAVIVWSEVTGEVNKITGDLPALDYRLAVGGKEEELGSGWAGL 168 +++ + + E + + V G Sbjct: 88 NIIKTCRILLEQHNGEVPEDRAAL---EALPGVGR------KTANVVLNTA------FGW 132 Query: 169 EGLMEGQPESFPAVASRRDDIAILAYTSGTTGNPKAVVHTHGWGYAHLKIVSSLWLDIRE 228 + ++ + + + + I Sbjct: 133 PTIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLILHGR--YTCIAR 190 Query: 229 SDTVWATAAPGWQKWIWSPFLTVLGNGATGF-VYNGPFHPDRFLQLLQDQGIQVLCCTPT 287 Sbjct: 191 250 Query: 288 EYRLMAKTEGLARYDLSKLRCAVSAGEPLNQEVINTFREHFDITIRDGYGQTESTLIIAA 347 Sbjct: 251 -- 308 Query: 348 LKDQPIRVGSMGKSIAPGIVEVIDGEGNPLPAGVVGDIAVHLSM--PALFKNYYKDPERK 405 Sbjct: 309 368 Query: 406 VNCSHGEYFVTGDRARKDEDGYFWFEGRGDDIIISSGYTIGPFEVEEALMKHALVKECAV 465 Sbjct: 369 428 Query: 466 VASPDEIRGSVVKAFVVLKDHSAGTPELVKELQSHVKEWTAPYKYPRKIEFIADLPKTAS 525 Sbjct: 429 488 Query: 526 GKIRRVELRDQE 537 Sbjct: 489 500 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.137 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 44,368,121 Number of extensions: 1984831 Number of successful extensions: 4942 Number of sequences better than 1.0e-05: 290 Number of HSP's gapped: 4136 Number of HSP's successfully gapped: 318 Length of query: 543 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 432 Effective length of database: 25,576,357 Effective search space: 11048986224 Effective search space used: 11048986224 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)