BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0078.0002.p (847 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2123 [G] COG0383 Alpha-mannosidase 269 2e-71 BH0791 [G] COG0383 Alpha-mannosidase 266 2e-70 TM1851 [G] COG0383 Alpha-mannosidase 258 2e-68 lin0424 [G] COG0383 Alpha-mannosidase 64 1e-09 TM1231 [G] COG0383 Alpha-mannosidase 59 3e-08 >lin2123 [G] COG0383 Alpha-mannosidase Length = 1032 Score = 269 bits (687), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 162/446 (36%), Positives = 233/446 (52%), Gaps = 30/446 (6%) Query: 62 FFRRTLAVPAEWAGQRAGLIFE---------SGGEGLLRVNGESRQGLDRNHTFAALEPS 112 + + T+ P+ G R F+ SG E LL VNGE QG+D+NH + Sbjct: 66 WLKTTITFPSAKEGTRLIGYFDFGNTGDGHNSGFESLLFVNGEPFQGVDQNHQEVLFPDN 125 Query: 113 PDGRPLELEIELFDPIPEPVDPLNQQAVIQPPVRAIRTMLVLVNEAVQSLMYSAVIVRDA 172 G+ EL L+ + P Q ++ + +N + L +++ Sbjct: 126 FAGKTTELVFRLWSGLEGGGVPSIQTHQLK------EAFIGYLNLVIDDLYFTSKATLKT 179 Query: 173 AVLLPEADMRRTRMLEALYRVIDGFLNMDETAVREGSAVAALEKK---LRADIAGIGGNA 229 L E + +L+A+ R +L +D ++++E L+A I + N Sbjct: 180 LDQLEENNPTHAPLLQAINR---AYLAIDWGTPGSNQNLSSMENANQILQAAIKEMPKNF 236 Query: 230 EGTIHMVGQSHIDIAWLWPARETVRKTSRTFSTVNALMEEYPEYQFAQSQPQLFQYLKDN 289 + VG +HID+AWLW + T K +R+FSTV LME+YPEY F QSQPQL+ Y+K++ Sbjct: 237 PIKVAAVGHTHIDVAWLWRLKHTREKAARSFSTVLHLMEDYPEYLFLQSQPQLYAYIKED 296 Query: 290 DPVLYAKVKERIREGRWELVGGMWVEPDLNIPSGESLMRQMLYGQRFYLEEFGKTSDIEW 349 P +YAK+K +I EG WE GGMW+E D NIPSGESL+RQMLYGQ+F EEFGK S W Sbjct: 297 YPEIYAKIKAKIEEGNWEADGGMWLEADCNIPSGESLVRQMLYGQKFLREEFGKPSSFLW 356 Query: 350 LPDTFGYCASLPQILRHGGVRYFMTTKLGWNDTNVFPYDLFHWVGIDGTSMLSYLNHGVN 409 LPD FGY +LPQIL G++ FMTTK+ WN N P+D F W GIDGT +L++ Sbjct: 357 LPDVFGYSWALPQILCKSGIKTFMTTKISWNQYNRMPHDTFQWRGIDGTEILTHFITAPE 416 Query: 410 ENT---------LPKDVHDHWQSFREKNIHNEQMLLYXXXXXXXXVTREMLEYIRRAELM 460 +N+ +++ W +++K I+ E +L Y V REMLE RR + M Sbjct: 417 KNSRISTYNGKMTARELVGLWDKYQDKEINQELLLAYGYGDGGGGVNREMLEMRRRYDKM 476 Query: 461 VGQPASTFSNAAGFFSGIGERQPQVD 486 G P +A +F+ + E D Sbjct: 477 PGIPEVKPKSATDYFNNLHETIQSTD 502 >BH0791 [G] COG0383 Alpha-mannosidase Length = 1039 Score = 266 bits (679), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 157/444 (35%), Positives = 236/444 (53%), Gaps = 35/444 (7%) Query: 62 FFRRTLAVPAEWAGQRAGLIFE---------SGGEGLLRVNGESRQGLDRNHTFAALEPS 112 + +L +P W+ +R L + SG E LL VN + QG+D NH L P Sbjct: 65 WLNTSLHLPEAWSDKRCVLFLDVGRTGGGNNSGFEALLFVNKQPFQGVDSNHQ-EVLLPK 123 Query: 113 PDGRPLELEIELFDPIPEPVDPLNQQAVIQPPVRAIRTMLVLVNEAVQSLMYSAVIVRDA 172 G LE+++ ++ + P Q R + +++ V + + A + +A Sbjct: 124 EGGNRLEIDVMMWSGLEGGGTPRTQY------YRFKQAWYGWLDQKVDAFYFQAKAILEA 177 Query: 173 AVLLPEADMRRTRMLEALYRVIDGFLNMDETAVRE--GSAVAALEKKLRADIAGIGGNAE 230 L D R ML L + +N + + S ALE+ L D+ + +++ Sbjct: 178 IGQLDVHDATRVAMLSRLNQAF-ASINWTQPGNEQFYDSVYEALER-LERDMEHLPKHSD 235 Query: 231 GTIHMVGQSHIDIAWLWPARETVRKTSRTFSTVNALMEEYPEYQFAQSQPQLFQYLKDND 290 IH +G SHID+AWLW + T K RTFSTV LM+ YP+Y + QSQPQL+Q++K+ + Sbjct: 236 VHIHAIGHSHIDLAWLWQTKHTKEKAKRTFSTVLRLMDLYPDYVYLQSQPQLYQWMKEEE 295 Query: 291 PVLYAKVKERIREGRWELVGGMWVEPDLNIPSGESLMRQMLYGQRFYLEEFGKTSDIEWL 350 P LY +K+++ +G+WE+ GGMWVE D NIPSGESL+RQ+LYG+RF+ +EF K S I WL Sbjct: 296 PKLYEAIKQKVDDGQWEVEGGMWVEADCNIPSGESLVRQLLYGKRFFKKEFHKESKILWL 355 Query: 351 PDTFGYCASLPQILRHGGVRYFMTTKLGWNDTNVFPYDLFHWVGIDGTSMLSYLNHGVNE 410 PD FGY +LPQIL+ G+ FMTTK+ WN N P D F+W G+DGT +L++ E Sbjct: 356 PDVFGYSWALPQILKKSGIDTFMTTKISWNQYNRMPNDTFYWRGLDGTEILTHFI-TTPE 414 Query: 411 NTLPKD--------------VHDHWQSFREKNIHNEQMLLYXXXXXXXXVTREMLEYIRR 456 PKD + W+ +R+K ++ + +L Y V REMLE R+ Sbjct: 415 PGRPKDSWFYTYNGLMSAETIKGTWEKYRDKAVNQDLLLAYGYGDGGGGVNREMLEMKRQ 474 Query: 457 AELMVGQPASTFSNAAGFFSGIGE 480 + + G P ++ +F + E Sbjct: 475 LDQVPGVPHVKSTHVKEYFHQLHE 498 >TM1851 [G] COG0383 Alpha-mannosidase Length = 1010 Score = 258 bits (660), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 164/459 (35%), Positives = 240/459 (52%), Gaps = 34/459 (7%) Query: 63 FRRTLAVPAEWAG-QRAGLIFESGGEGLLRVNGESRQGLDRNHTFAALEPSPDGRPLELE 121 FR+ L P AG QRA L GGE L+ ++G+ ++ H + P DG+P +E Sbjct: 52 FRKELE-PFSVAGEQRAYLKLWFGGETLVLIDGKPYGEINEYHRMLNITPLADGKPHTIE 110 Query: 122 IELF--DPIPEPVDPLNQQA----VIQPPVRAIRTMLVLVN--EAVQSLMYSAVIVRDAA 173 ++ +P P+ +A V + ++ ++T+ + + E ++ S ++ + Sbjct: 111 AQVMPRGLFGKPEKPVFTEAFFIVVDEALMKVVKTLELTIKTAEVIEDESLSKKLLDISE 170 Query: 174 VLLPEADMRR---TRMLEALYR--VIDGFLNMDET-AVREGSAVAALEK----------K 217 L + + R T ++ AL + D N T +E + V E+ K Sbjct: 171 EFLSKVWIPRDTGTYLMTALEDPGIKDEIKNTWNTPEFKEFTGVKLPEELRNQILEEFEK 230 Query: 218 LRADIAGIGGN--AEGTIHMVGQSHIDIAWLWPARETVRKTSRTFSTVNALMEEYPEYQF 275 + + I N GTIH+VG +HID AWLWP ET RK RTF+ L + YPE+ + Sbjct: 231 FKEKLDRIRKNHPGFGTIHLVGHAHIDYAWLWPVEETKRKILRTFANSVLLSKLYPEFVY 290 Query: 276 AQSQPQLFQYLKDNDPVLYAKVKERIREGRWELVGGMWVEPDLNIPSGESLMRQMLYGQR 335 QS Q+++ LK N P L+ +V++ + EGRWE VGGMWVE D N+PS ESL+RQ YGQ+ Sbjct: 291 TQSSAQMYEDLKQNSPELFEEVRKLVEEGRWEPVGGMWVESDCNVPSIESLVRQFYYGQK 350 Query: 336 FYLEEFGKTSDIEWLPDTFGYCASLPQILRHGGVRYFMTTKLGWNDTNVFPYDLFHWVGI 395 F+ EFGK S + WLPD FG+ LPQIL+ G++YF+TTKL WNDTN FPYDL W GI Sbjct: 351 FFEREFGKKSKVCWLPDVFGFSWVLPQILKEAGIKYFVTTKLNWNDTNEFPYDLCRWRGI 410 Query: 396 DGTSMLSYL----NHGVNENTLPKDVHDHWQSFREKNIHNEQMLLYXXXXXXXXVTREML 451 DG+ ++ + N G N P V+ W++FR+K++ N +L + T EML Sbjct: 411 DGSEVIYFSFKNPNEGYNGKIDPDTVYKTWKNFRQKDLTNRVLLSFGHGDGGGGPTEEML 470 Query: 452 EYIRRAELMVGQPASTFSNAAGFFSG--IGERQPQVDGQ 488 E + G P FF I E P DG+ Sbjct: 471 ENYEVLKDFPGLPHLEMGTVEEFFKKVEIDEELPVWDGE 509 >lin0424 [G] COG0383 Alpha-mannosidase Length = 875 Score = 63.5 bits (153), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 50/177 (28%), Positives = 82/177 (46%), Gaps = 8/177 (4%) Query: 229 AEGTIHMVGQSHIDIAWLWPARETVRKTSRTFSTVNALMEEYPEYQF--AQSQPQLFQYL 286 A+ +H++ SH D W + + + + V +E Y F +Q L + Sbjct: 2 AKTKVHVIPHSHWDREWYFTSSRSTIYLVKHLKEVIETLEAKDNYHFYLMDAQSSLIEDY 61 Query: 287 KDNDPVLYAKVKERIREGRWELVGGMW-VEPDLNIPSGESLMRQMLYGQRFYLEEFGKTS 345 P ++++ I E R L+ G W + D + S ES++R +LYG R E G + Sbjct: 62 LRYCPEDKTRLEKLIAEKR--LITGPWYTQTDQLVISQESIVRNLLYGTRI-AREMGHSM 118 Query: 346 DIEWLPDTFGYCASLPQILRHGGVRYFMTTKLGWNDTNVFPYDLFHWVGIDGTSMLS 402 + ++PD FG ++PQI + G+ F+ + G D N F W G DGT ML+ Sbjct: 119 AVGYVPDAFGQGGNMPQIYKEFGISKFLFWR-GVAD-NRLKQTEFIWRGDDGTEMLA 173 >TM1231 [G] COG0383 Alpha-mannosidase Length = 832 Score = 58.9 bits (141), Expect = 3e-08, Method: Compositional matrix adjust. Identities = 44/170 (25%), Positives = 81/170 (47%), Gaps = 6/170 (3%) Query: 233 IHMVGQSHIDIAWLWPARETVRKTSRTFSTVNALMEEYPEYQFA-QSQPQLFQYLKDNDP 291 + +V +H D W + T + F V L+++ PE+ + Q + L P Sbjct: 3 VKVVVHNHWDREWFTSSEVTSKWLKEVFFRVKELVQKNPEFVYVLDGQTAAVEDLLVYHP 62 Query: 292 VLYAKVKERIREGRWELVGGMWVEPDLNIPSGESLMRQMLYGQRFYLEEFGKTSDIEWLP 351 L ++E +R GR LVG +++ D IP S+++ G++ FG+ + WL Sbjct: 63 DLEEDLRELVRSGRL-LVGPYYIQIDWRIPGEASILKNFEIGEK-DTNRFGRRMNAGWLL 120 Query: 352 DTFGYCASLPQILRHGGV-RYFMTTKLGWNDTNVFPYDLFHWVGIDGTSM 400 D+FG+ + PQ+ R G+ + F+ + + + + F W G DGT++ Sbjct: 121 DSFGHISQEPQLHRIFGIEKVFLWRGISFENDGI--SQEFFWKGSDGTAV 168 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.140 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 65,481,885 Number of extensions: 2887806 Number of successful extensions: 7724 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 7713 Number of HSP's successfully gapped: 8 Length of query: 847 Length of database: 38,378,431 Length adjustment: 115 Effective length of query: 732 Effective length of database: 25,115,021 Effective search space: 18384195372 Effective search space used: 18384195372 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 120 (50.8 bits)