BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0074.0005.p (749 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3819 [G] COG3866 Pectate lyase 92 3e-18 STM0433 [R] COG0693 Putative intracellular protease/amidase 85 5e-16 BS_pelB [G] COG3866 Pectate lyase 74 7e-13 BS_pel [G] COG3866 Pectate lyase 74 7e-13 BH0698 [G] COG3866 Pectate lyase 62 3e-09 >BH3819 [G] COG3866 Pectate lyase Length = 333 Score = 92.4 bits (228), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 62/210 (29%), Positives = 110/210 (52%), Gaps = 18/210 (8%) Query: 224 PLAIRIIGKVTAADLSGQLNSSGYLEVKGKNNYSQMNMTIEGIGNDAYAYGWGMLLRYVG 283 PL I + G +T ++ S +++K +N S I G+G + G G+ + Sbjct: 75 PLTIYVNGTITPSNTS-----DSKIDIKDVSNVS-----ILGVGTNGRLNGIGIKVWRAN 124 Query: 284 NVEVRNLGVMLF---PDDGISMDTGNVNVWVHNNDIFYGSAGSDADQAKGDGSTDLKKGS 340 N+ +RNL + D IS++ + N+WV +N+++ A + + DG D+K+ + Sbjct: 125 NIIIRNLTIHEVHTGDKDAISIEGPSRNIWVDHNELY---ASLNVHKDHYDGLFDVKRDA 181 Query: 341 TYITISYNHYWDSGKASLVGLSEPAEF--FVTFHHNWFDHSDSRHPRIRVASVHVYNNFY 398 IT S+N+ D KA L+G S+ + +TFHHN+F + +SR P R H+++N++ Sbjct: 182 YNITFSWNYVHDGWKAMLMGNSDSDNYDRNITFHHNYFKNLNSRVPAYRFGKAHLFSNYF 241 Query: 399 DGVSKYGVGVTTGASAFVESNVFRHAKYPM 428 + + + G+ GA VE NVF +A P+ Sbjct: 242 ENILETGINSRMGAEMLVEHNVFENATNPL 271 >STM0433 [R] COG0693 Putative intracellular protease/amidase Length = 196 Score = 84.7 bits (208), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 0/189 (0%), Positives = 0/189 (0%), Gaps = 38/189 (20%) Query: 260 NMTIEGIGNDAYAYGWGMLLRYVGNVEVRNLGVMLF--PDD--GISMDTGNVNV------ 309 + + + A +L+ + G + Sbjct: 96 HRSGRIV-AAICAAAATVLVPHDIFPIGNMTGFPALKDKIPAEQWLDKRVVWDARVKLLT 154 Query: 310 WVHNNDIFYGSAGSDADQAKGDGSTDLKKGSTYITISYNHYWDSGKASLVGLSEPAE--- 366 Sbjct: 155 SQGPGTAIDFG---------- 204 Query: 367 ----FFVTFHHNWFDHSDSRHPRIRVASVHVYNNFYD-----GVSK-----YGVGVTTGA 412 Sbjct: 205 264 Query: 413 SAFVESNVF 421 Sbjct: 265 273 >BS_pelB [G] COG3866 Pectate lyase Length = 420 Score = 74.3 bits (181), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 51/165 (30%), Positives = 83/165 (50%), Gaps = 18/165 (10%) Query: 331 DGSTDLKKGSTYITISYNHYWDSGKASLVG-----LSEPAEFFVTFHHNWFDHSDSRHPR 385 DG TD G+ YIT+SYN+Y D K+S+ G S+ + +T HHN + + R PR Sbjct: 244 DGQTDASNGANYITMSYNYYHDHDKSSIFGSSDSKTSDDGKLKITLHHNRYKNIVQRAPR 303 Query: 386 IRVASVHVYNNFYDGVS-------KYGVGVTTGASAFVESNVFRHAKYPMMIALQGTDAL 438 +R VHVYNN+Y+G + Y G+ + + ++NV P + A + Sbjct: 304 VRFGQVHVYNNYYEGSTSSSSYPFSYAWGIGKSSKIYAQNNVI---DVPGLSAAKTISVF 360 Query: 439 GEGTFSGENGGMIKAYNNTVVDAASLIYANSNAGTAPANTTSFDA 483 GT ++G ++ N T ++A++ +S+ G P+ S DA Sbjct: 361 SGGTALYDSGTLL---NGTQINASAANGLSSSVGWTPSLHGSIDA 402 >BS_pel [G] COG3866 Pectate lyase Length = 420 Score = 74.3 bits (181), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 51/165 (30%), Positives = 83/165 (50%), Gaps = 18/165 (10%) Query: 331 DGSTDLKKGSTYITISYNHYWDSGKASLVG-----LSEPAEFFVTFHHNWFDHSDSRHPR 385 DG TD G+ YIT+SYN+Y D K+S+ G S+ + +T HHN + + R PR Sbjct: 244 DGQTDASNGANYITMSYNYYHDHDKSSIFGSSDSKTSDDGKLKITLHHNRYKNIVQRAPR 303 Query: 386 IRVASVHVYNNFYDGVS-------KYGVGVTTGASAFVESNVFRHAKYPMMIALQGTDAL 438 +R VHVYNN+Y+G + Y G+ + + ++NV P + A + Sbjct: 304 VRFGQVHVYNNYYEGSTSSSSYPFSYAWGIGKSSKIYAQNNVI---DVPGLSAAKTISVF 360 Query: 439 GEGTFSGENGGMIKAYNNTVVDAASLIYANSNAGTAPANTTSFDA 483 GT ++G ++ N T ++A++ +S+ G P+ S DA Sbjct: 361 SGGTALYDSGTLL---NGTQINASAANGLSSSVGWTPSLHGSIDA 402 >BH0698 [G] COG3866 Pectate lyase Length = 345 Score = 62.4 bits (150), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 34/117 (29%), Positives = 58/117 (49%), Gaps = 16/117 (13%) Query: 327 QAKGDGSTDLKKGSTYITISYNHYWDSGKASLVGLSE----PAEFFVTFHHNWFDHSDSR 382 + GDG D+ G+ Y+TIS + + K+ + ++ ++ VT WF + R Sbjct: 166 RKHGDGLIDIVNGANYVTISNSRFEQHNKSITISGNDNDTNTDKYKVTIQDCWFRGTTQR 225 Query: 383 HPRIRVASVHVYNNFYDGVSKYG------------VGVTTGASAFVESNVFRHAKYP 427 +PR+R VH+YNN+Y + KYG +GV A + E+N F + ++P Sbjct: 226 NPRVRFGMVHLYNNYYSDMGKYGRDMGYSSSLGYAIGVGVSAKIYSENNYFENLRHP 282 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.129 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 48,703,550 Number of extensions: 2043400 Number of successful extensions: 2932 Number of sequences better than 1.0e-05: 5 Number of HSP's gapped: 2922 Number of HSP's successfully gapped: 5 Length of query: 749 Length of database: 38,378,431 Length adjustment: 114 Effective length of query: 635 Effective length of database: 25,230,355 Effective search space: 16021275425 Effective search space used: 16021275425 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 120 (50.8 bits)