BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0074.0004.p (461 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SA0132 [GEPR] COG0477 Permeases of the major facilitator superfa... 112 2e-24 MTH104 [GEPR] COG0477 Permeases of the major facilitator superfa... 82 3e-15 MTH1049 [K] COG0085 DNA-directed RNA polymerase, beta subunit/14... 82 3e-15 lin1658 [GEPR] COG0477 Permeases of the major facilitator superf... 78 3e-14 BH0554 [GEPR] COG0477 Permeases of the major facilitator superfa... 72 2e-12 >SA0132 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 450 Score = 112 bits (279), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 92/407 (22%), Positives = 182/407 (44%), Gaps = 2/407 (0%) Query: 8 SVSKSMVWLSILAFFSVFNETVFNVSLPDIARQFDIRPSAANWVNTSFMISFAVGSALYG 67 + K++ L L FS+ + F + IA +I + +W T + +G+ +Y Sbjct: 7 KIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISVTTVSWQATLAGLVIGIGAVVYA 66 Query: 68 KISDXXXXXXXXXXXXXXXXXXXXIGIVFHKWFLVVLIARFIQGAGASAVPALIMVFVAR 127 +SD IG +F F ++L+ R IQ AG +A L +++VA+ Sbjct: 67 SLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLLLVGRIIQTAGLAAAETLYVIYVAK 126 Query: 128 YIEPNNKGKAFGMIGSMVAFSEGIGPAVGGVIADYIHWSYLFILPMMTLLTLPFFLHVLP 187 Y+ ++ G+ S + S IG GG I+ Y+HW+ +F++ ++ + TLPF +LP Sbjct: 127 YLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFISTYLHWTNMFLIALIVVFTLPFLFKLLP 186 Query: 188 DE-PSKKGKFDVLGASLLSVGIVMFMLFTSMYQWGYLAISVLCFFGFLQRIRRAKQPFIE 246 E + K D +G L++ MLF + + W Y+ +++ F I+ A++P + Sbjct: 187 KENNTNKAHLDFVGLILVATIATTVMLFITNFNWLYMIGALIAIIVFALYIKNAQRPLVN 246 Query: 247 PSLLRRKKFXXXXXXXXXXXXXXXXYISTVPYMMKDVYLMPTSLIGSAVLFPGTISVLLF 306 S + K++ YI T P++M+ +Y + S +L PG I ++ Sbjct: 247 KSFFQNKRYASFLFIVFVMYAIQLGYIFTFPFIMEQIYHLQLD-TTSLLLVPGYIVAVIV 305 Query: 307 GFVSGILVDKRGCFFTMLVGLFMLVVSFLSISLFVDRTPWLISTTMVLTFGGLSFIKTVV 366 G +SG + + ++ + ++ +S + + V + +M+ G + + + Sbjct: 306 GALSGKIGEYLNSKQAIITAIILIALSLILPAFAVGNHISIFVISMIFFAGSFALMYAPL 365 Query: 367 STSVAEALSSDESGSGMSFLNFACFLAEGIGVSYAGGLLTMSWLKFP 413 + + + +G + F N +A +G++ A L+ L FP Sbjct: 366 LNEAIKTIDLNMTGVAIGFYNLIINVAVSVGIAIAAALIDFKALNFP 412 >MTH104 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 268 Score = 81.6 bits (200), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 0/400 (0%), Positives = 0/400 (0%), Gaps = 19/400 (4%) Query: 21 FFSVFNETVFNVSLPDIARQFDIRPSAANWVNTSFMISFAVGSALYGKISDXXXXXXXXX 80 + + R + Sbjct: 19 GDLKIKTYKVVLIARAASIFGVKRIVIYHDDADGEARFIRDILTYMDTPQYLRRKVFPIM 78 Query: 81 XXXXXXXXXXXIGIVFHKWFLVVLIARFIQGAGASAVPALIMVFVARYIEPNNKGKAFGM 140 + H V + QG V +V + + K Sbjct: 79 RELKHVGILPPLRTPHHPTGKPVT-GEYRQGLTVKRVKKGTLVDIGADKLALCREKLTVN 137 Query: 141 IGSMVAFSEGIGPAVGGVIADYIHWSYLFI--LPMMTLLTLPFFLHVLPDEPSKKGKFDV 198 + + + L + + + + Sbjct: 138 RIMSFRVVRLGKEILIEPDEPE-DRYWGYEVLDTRRNLAESLKTVGADVVVATSRNASPI 196 Query: 199 LGASLLSVGIVMFMLFTSMYQWGYLAISVL------------CFFGFLQRIRRAK-QPFI 245 + ++ G ++ + Sbjct: 197 TSILDEVKTRMRGAREAAILFGGPYKGLPEIDADIWVNTLPGQCTETVRTEEAVLATLSV 256 Query: 246 EPSLLRRKKFXXXXXXXXXXXXXXXXYISTVPYMMKDVYLMPTSLIGSAVLFPGTISVLL 305 Sbjct: 257 -- 314 Query: 306 FGFVSGILVDKRGCFFTMLVGLFMLVVSFLSISLFVDRTPWLISTTMVLTFGGLSFIKTV 365 Sbjct: 315 374 Query: 366 VSTSVAEALSSDESGSGMSFLNFACFLAEGIGVSYAGGLL 405 Sbjct: 375 414 >MTH1049 [K] COG0085 DNA-directed RNA polymerase, beta subunit/140 kD subunit Length = 268 Score = 81.6 bits (200), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 0/400 (0%), Positives = 0/400 (0%), Gaps = 19/400 (4%) Query: 21 FFSVFNETVFNVSLPDIARQFDIRPSAANWVNTSFMISFAVGSALYGKISDXXXXXXXXX 80 + + R + Sbjct: 19 GDLKIKTYKVVLIARAASIFGVKRIVIYHDDADGEARFIRDILTYMDTPQYLRRKVFPIM 78 Query: 81 XXXXXXXXXXXIGIVFHKWFLVVLIARFIQGAGASAVPALIMVFVARYIEPNNKGKAFGM 140 + H V + QG V +V + + K Sbjct: 79 RELKHVGILPPLRTPHHPTGKPVT-GEYRQGLTVKRVKKGTLVDIGADKLALCREKLTVN 137 Query: 141 IGSMVAFSEGIGPAVGGVIADYIHWSYLFI--LPMMTLLTLPFFLHVLPDEPSKKGKFDV 198 + + + L + + + + Sbjct: 138 RIMSFRVVRLGKEILIEPDEPE-DRYWGYEVLDTRRNLAESLKTVGADVVVATSRNASPI 196 Query: 199 LGASLLSVGIVMFMLFTSMYQWGYLAISVL------------CFFGFLQRIRRAK-QPFI 245 + ++ G ++ + Sbjct: 197 TSILDEVKTRMRGAREAAILFGGPYKGLPEIDADIWVNTLPGQCTETVRTEEAVLATLSV 256 Query: 246 EPSLLRRKKFXXXXXXXXXXXXXXXXYISTVPYMMKDVYLMPTSLIGSAVLFPGTISVLL 305 Sbjct: 257 -- 314 Query: 306 FGFVSGILVDKRGCFFTMLVGLFMLVVSFLSISLFVDRTPWLISTTMVLTFGGLSFIKTV 365 Sbjct: 315 374 Query: 366 VSTSVAEALSSDESGSGMSFLNFACFLAEGIGVSYAGGLL 405 Sbjct: 375 414 >lin1658 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 489 Score = 77.8 bits (190), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 93/412 (22%), Positives = 174/412 (42%), Gaps = 18/412 (4%) Query: 3 KSNSGSVSKSMVWLSIL--AFFSVFNETVFNVSLPDIARQFDIRPSAANWVNTSFMISFA 60 K+ S SV ++ + + +L AF ++ N+T+ NV+LP I + F I S W++T FM+ Sbjct: 2 KAASTSVKRNGILIVMLMGAFVTILNQTLMNVALPSIMKDFGITASQGQWLSTGFMLVNG 61 Query: 61 VGSALYGKISDXXXXXXXXXXXXXXXXXXXXIGIVFHKWFLVVLIARFIQGAGASAVPAL 120 V + + + IG F + +++ R +Q GA V L Sbjct: 62 VMIPMTAFLIERFTTRQLYLFAMITFAIGTAIG-GFATDYTMLIAGRMVQAIGAGIVMPL 120 Query: 121 IMVFVARYIEPNNKGKAFGMIGSMVAFSEGIGPAVGGVIADYIHWSYLF--ILPMMTLLT 178 + V V +G+A G+IG + F+ IGP + G I W LF I+P +L Sbjct: 121 LTVVVLNLFPMERRGRAMGLIGLAMNFAPAIGPTLSGWIVQQYDWRNLFFIIIP-FAILD 179 Query: 179 LPFFLHVLPDEPSKK-GKFDVLGASLLSVGIVMFML-FTSM--YQW------GYLAISVL 228 + + +L + + K D+LG + +VG +L F++ + W G++ + ++ Sbjct: 180 IVVAIFLLKNVGKRTFPKLDILGVIMSTVGFGSLLLGFSNAGDHDWLTWKVAGFIILGLV 239 Query: 229 CFFGFLQRIRRAKQPFIEPSLLRRKKFXXXXXXXXXXXXXXXXYISTVPYMMKDVYLMPT 288 F++ K P + + + F + +P ++ V + Sbjct: 240 VLGIFIRYQSSNKAPLLNFKVFKYPTFALTTAISFFVVMGLFGGMLLLPIFLQTVRGF-S 298 Query: 289 SLIGSAVLFPGTISVLLFGFVSGILVDKRGCFFTMLVGLFMLVVS-FLSISLFVDRTPWL 347 L VL PG + + V+G++ D+ G + LVGL ++ S F+ +L T Sbjct: 299 PLESGLVLLPGALVTAILSPVTGVMFDRFGAKYLSLVGLIIMAGSTFMFTNLDESTTLTF 358 Query: 348 ISTTMVLTFGGLSFIKTVVSTSVAEALSSDESGSGMSFLNFACFLAEGIGVS 399 I + G++ + + T+ +L + G + N +A IG + Sbjct: 359 IIIVQTIRSAGMAMVMMPLQTAALNSLPLKLASHGSAMFNTMRQVAGSIGTA 410 >BH0554 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 474 Score = 72.0 bits (175), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 93/393 (23%), Positives = 159/393 (40%), Gaps = 21/393 (5%) Query: 10 SKSMVWLSIL--AFFSVFNETVFNVSLPDIARQFDIRPSAANWVNTSFMISFAVGSALYG 67 K MV ++L +F + N ++ N ++P + F+ A WV T FM++ + L G Sbjct: 6 KKWMVVCAVLFGSFTMILNNSMLNPAIPQLMNVFEADAVATGWVITIFMVAMGMTMPLTG 65 Query: 68 KISDXXXXXXXXXXXXXXXXXXXXIGIVFHKWFLVVLIA-RFIQGAGASAVPALIMVFVA 126 + D +G + W L LI R +QG G + L M + Sbjct: 66 YLGDKIGKKEAYILGLGIFVLGSLLGAL--SWNLPSLIVFRGLQGIGGGVMMPLSMTLIF 123 Query: 127 RYIEPNNKGKAFGMIGSMVAFSEGIGPAVGGVIADYIHWSYLFI--LPMMTLLTLPFFLH 184 N +G A G+ G + IGP +GG I + +W YLF+ +P L + ++ Sbjct: 124 DAFPRNERGLATGVWGVASMMAPTIGPTLGGFIVETSNWKYLFLVNIPFGLLGIIAAVIY 183 Query: 185 VLPDEPSKKGKFDVLGASLLSVGIVMFML-------FTSMYQW---GYLAISVLCFFGFL 234 + S + K D G ++ G+ +L T + +W L + LC + F+ Sbjct: 184 LPKIARSGQIKLDRWGFLFVTAGVGSILLAFGRMSDLTHLTEWINGVLLLLGALCLYVFV 243 Query: 235 QRIRRAKQPFIEPSLLRRKKFXXXXXXXXXXXXXXXXYISTVPYMMKDVYLMPTSLIGSA 294 Q +RA+QP ++ SL R + I VP +++ VY + G Sbjct: 244 QVEKRAEQPLLDLSLFRIPAYSLSIWVAGISSIGLFAGIFLVPLLLQQVYDYGPIMTG-L 302 Query: 295 VLFPGTISVLLFGFVSGILVDKRGCFFTMLVGLFMLVVSFLSIS-LFVDRTPWLISTTMV 353 V P + L + G ++DKRG M G+ + V ++ L ++ W I M Sbjct: 303 VFLPSALFTGLTMSIGGRMLDKRGPSGIMTAGMMIAAVGTFALGYLHLETGLWYIFAWMA 362 Query: 354 LTFGGLSFIKTVVSTSVAEALSSD--ESGSGMS 384 + G+ +T+ A+ GS M+ Sbjct: 363 IRGVGMGLTTMPATTTGMNAIPEGLISRGSAMN 395 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.329 0.142 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,993,470 Number of extensions: 1153260 Number of successful extensions: 4892 Number of sequences better than 1.0e-05: 44 Number of HSP's gapped: 4866 Number of HSP's successfully gapped: 44 Length of query: 461 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 351 Effective length of database: 25,691,691 Effective search space: 9017783541 Effective search space used: 9017783541 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 118 (50.1 bits)