BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0069.0009.p (341 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ywcH [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 286 4e-77 BS_yceB [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 202 5e-52 RSc2462 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 189 4e-48 SA0312 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetr... 166 7e-41 VNG0930G [C] COG2141 Coenzyme F420-dependent N5,N10-methylene te... 163 3e-40 >BS_ywcH [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 286 bits (732), Expect = 4e-77, Method: Compositional matrix adjust. Identities = 22/332 (6%), Positives = 41/332 (12%), Gaps = 5/332 (1%) Query: 1 MMKLSILDQSPVSEGSTPAEALAETASLAIEADRLGYHRFWVSEHHAARGLAGSSPEVLM 60 M I S + +A + + P Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 61 AHLAAVTSRIRIGSGAVLLPHYSAYKVAENFRVLEALYPGRIDXXXXXXXXXXXXXXXXX 120 T + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 121 QENRTGG--SAELYELQIQDLLSYLYGAPDQSHRFAGLETSPQISTAPELWXXXXXXXXX 178 + + + + + + E Sbjct: 121 LQ---TDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIG 177 Query: 179 XXXXXXXTGYAYARFINSAGGSMAVXXXXXXXXXXXXXXXXQALVSVFAVCAETSAEADR 238 Y + V A VS + + Sbjct: 178 VSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLG 237 Query: 239 LAASMDLSLVQLEKGHVSTGTPSVETALGYKYTPYDRFRIEDNRRRMVLGSPDEVRRQFT 298 L + S T + T D RR L Sbjct: 238 FGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVR 297 Query: 299 ALMEEYQCEEIMFATLMHRYEDKLRSLRLIAE 330 + + L L + Sbjct: 298 RQPFVASTLLGATTMDQLKTNIESLHLELSED 329 >BS_yceB [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 202 bits (515), Expect = 5e-52, Method: Compositional matrix adjust. Identities = 26/310 (8%), Positives = 44/310 (14%), Gaps = 2/310 (0%) Query: 22 LAETASLAIEADRLGYHRFWVSEHHAARGLAGSSPEVLMAHLAAVTSRIRIGSGAVLLPH 81 S + + ++ A A V + + Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 82 YSAYKVAENFRVLEALYPGRIDXXXXXXXXXXXXXXXXXQENRTGGSAELYELQIQDLLS 141 + V + I Q E L Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIRE-ALHDSLKRLQ 122 Query: 142 YLYGAPDQSHRFAGLETSPQISTAPELWXXXXXXXXXXXXXXXXTGYAYARFINSAGGSM 201 Y Q H A Sbjct: 123 TDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNET 182 Query: 202 AVXXXXXXXXXXXXXXXXQALVSVFAVCAETSAEADRLAASMDLSLVQLEKGHVSTGTPS 261 A + S E S + L Sbjct: 183 AFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYS-CLGFGTL 241 Query: 262 VETALGYKYTPYDRFRIEDNRRRMVLGSPDEVRRQFTALMEEYQCEEIMFATLMHRYEDK 321 L R + R + + + + + A R + Sbjct: 242 TGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPF 301 Query: 322 LRSLRLIAEV 331 + S L A Sbjct: 302 VASTLLGATT 311 >RSc2462 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 189 bits (481), Expect = 4e-48, Method: Compositional matrix adjust. Identities = 15/336 (4%), Positives = 45/336 (13%), Gaps = 9/336 (2%) Query: 2 MKLSILDQSPVSEGSTPAEALAETASLAIEADRLGYHRFWVSEHHAARGLAGSSPEVLMA 61 S+ + T E +E + A + + + L Sbjct: 7 PHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTE 66 Query: 62 HLAAVTSRIRIGSGAVLLPHYSAYKVAENFRVLEALYPGRIDXXXXXXX---XXXXXXXX 118 +++ + N + + Sbjct: 67 TYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYL 126 Query: 119 XXQENRTGGSAELYELQIQDLLSYLYGAPDQSHRFAGLETSPQISTAPELWXXXXXXXXX 178 + L Y + + + Sbjct: 127 DLYQVH----WPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNET 182 Query: 179 XXXXXXXTGYAYARFINSAGGSMAVXXXXXXXXXXXXXXXXQALVSVFAVCAETSAEADR 238 A + V + Sbjct: 183 AFGVMRYLHLADKHDLPRIVTIQ-NPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTL 241 Query: 239 LAASMDLSLVQLEKGHVSTGTPSVETALGYKYTPYDRFRIEDNRRRMVLGSPDEVRRQFT 298 ++ + + + + + I R+ Sbjct: 242 TGKYLNGAKPAGARN-TLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQP 300 Query: 299 ALMEEYQCEEIMFATLMHRYEDKLRSLRLIAEVCGL 334 + M + L + Sbjct: 301 FVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEA 336 >SA0312 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 166 bits (419), Expect = 7e-41, Method: Compositional matrix adjust. Identities = 16/336 (4%), Positives = 43/336 (12%), Gaps = 10/336 (2%) Query: 1 MMKLSILDQSPVSEGSTPAEALAETASLAIEADRLGYHRFWVSEHHAARGLAGSSPEVLM 60 M I S + +A + + P Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 61 AHLAAVTSRIRIGSGAVLLPHYSAYKVAENFRVLEALYPGRIDXXXXXXXXXXXXXXXXX 120 T + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 121 QENRTGGSAELYELQIQDLLSYLYGAPDQSHRFAGLETSPQISTAPELWXXXXXXXXXXX 180 + Y++ + +G S + + + Sbjct: 121 LQTDYLDL---YQVHWPQRPTNCFGKLGYSWTDSAPA--VSLLDTLDALAEYQRAGKIRY 175 Query: 181 XXXXXTGYAYARF----INSAGGSM-AVXXXXXXXXXXXXXXXXQALVSVFAVCAETSAE 235 + + V + Sbjct: 176 IGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSC 235 Query: 236 ADRLAASMDLSLVQLEKGHVSTGTPSVETALGYKYTPYDRFRIEDNRRRMVLGSPDEVRR 295 + G +T G + ++ RR + + + Sbjct: 236 LGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAF 295 Query: 296 QFTALMEEYQCEEIMFATLMHRYEDKLRSLRLIAEV 331 + + L + Sbjct: 296 VRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVL 331 >VNG0930G [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 163 bits (413), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 16/339 (4%), Positives = 43/339 (12%), Gaps = 7/339 (2%) Query: 2 MKLSILDQSPVSEGSTPAEALAETASLAIEADRLGYHRFWVSEHHAARGLAGSSPEVLMA 61 M+ + S + + + + + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 62 HLAAVTSRIRIGSGAVLLPHYSAYKVAENFRVLEALYPGRIDXXXXXXXXXXXXXXXXXQ 121 + + + R D + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 122 ENRTGGSAELYELQIQDLLSYLYGAPDQSHRFAGLETSPQISTAPELWXXXXXXXXXXXX 181 + + + + + E Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 182 XXXXTGYAYARFINSAGGSMAVXX---XXXXXXXXXXXXXXQALVSVFAVCAETSAEADR 238 Y + V + + A + Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGT 240 Query: 239 LAASMDLSLVQLEKGHVSTGTPSVETA---LGYKYTPYDRFRIEDNRRRMVLGSPDEVRR 295 L + + + Y + R+ Sbjct: 241 LTGKYLNGAKPAGARNTLF-SRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQ 299 Query: 296 QFTALMEEYQCEEIMFATLMHRYEDKLRSLRLIAEVCGL 334 F A T + +L L Sbjct: 300 PFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAVH 338 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.132 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,796,454 Number of extensions: 747751 Number of successful extensions: 1343 Number of sequences better than 1.0e-05: 24 Number of HSP's gapped: 1331 Number of HSP's successfully gapped: 24 Length of query: 341 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 234 Effective length of database: 26,037,693 Effective search space: 6092820162 Effective search space used: 6092820162 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)