BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0069.0008.p (158 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value slr1171 [O] COG0386 Glutathione peroxidase 184 6e-47 ML0198 [K] COG1278 Cold shock proteins 178 3e-45 CAC1570 [O] COG0386 Glutathione peroxidase 178 3e-45 YBR244w [O] COG0386 Glutathione peroxidase 176 1e-44 CAC1549 [O] COG0386 Glutathione peroxidase 174 5e-44 >slr1171 [O] COG0386 Glutathione peroxidase Length = 169 Score = 184 bits (466), Expect = 6e-47, Method: Compositional matrix adjust. Identities = 89/162 (54%), Positives = 109/162 (67%), Gaps = 5/162 (3%) Query: 2 SIYSFAGVKPSGAEVPFKDYEGKVLLIANTASKCGLTPQYGDLQKLYEQYGDKGLVVLGF 61 +IY F+ G+ V +D+EGKVLLI NTAS+CG TPQY LQ LY ++GD+G VLGF Sbjct: 8 TIYGFSANALDGSPVALRDFEGKVLLIVNTASQCGFTPQYQGLQALYNRFGDRGFTVLGF 67 Query: 62 PCNQFAGQEPGTSEEAEEFCQINYGVTFPIFAKVDVNGPDASLLFQYLKGQQPGE----- 116 PCNQF QEPG S E + FC+ YGVTFP+F KV+VNGP+A LF++L PG Sbjct: 68 PCNQFGQQEPGGSGEIKNFCETRYGVTFPLFEKVEVNGPNAHPLFKFLTAASPGMAIPFL 127 Query: 117 GGSSDIQWNFTKFLVDRSGNVVARVEPKESPESMKEQIEALL 158 GG+ DI+WNFTKFLVDR G VV R P+ + IE LL Sbjct: 128 GGAEDIKWNFTKFLVDRQGKVVKRYGSIAKPDEIAADIEKLL 169 >ML0198 [K] COG1278 Cold shock proteins Length = 147 Score = 178 bits (451), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 81/147 (55%), Positives = 107/147 (72%), Gaps = 1/147 (0%) Query: 12 SGAEVPFKDYEGKVLLIANTASKCGLTPQYGDLQKLYEQYGDKGLVVLGFPCNQFAGQEP 71 +G V D++GKV+++ NTASKCG TPQ+ L+KLYE Y D+GL +LGFPCNQF Q+ Sbjct: 2 NGETVSMSDFKGKVVIVVNTASKCGFTPQFEGLEKLYENYKDQGLEILGFPCNQFVNQDA 61 Query: 72 GTSEEAEEFCQINYGVTFPIFAKVDVNGPDASLLFQYLKGQQPGEGGSSDIQWNFTKFLV 131 G + E EFCQ+NYGVTFP+F K+ VNG +A L+Q+LK + G S I+WNFTKFL+ Sbjct: 62 GENSEINEFCQLNYGVTFPMFQKIKVNGKEAHPLYQFLKKEAKG-ALSGTIKWNFTKFLI 120 Query: 132 DRSGNVVARVEPKESPESMKEQIEALL 158 DR GNV+ R PK P+ M+E+I+ LL Sbjct: 121 DREGNVIERFAPKTEPKEMEEEIQKLL 147 >CAC1570 [O] COG0386 Glutathione peroxidase Length = 159 Score = 178 bits (451), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 84/158 (53%), Positives = 110/158 (69%), Gaps = 1/158 (0%) Query: 1 MSIYSFAGVKPSGAEVPFKDYEGKVLLIANTASKCGLTPQYGDLQKLYEQYGDKGLVVLG 60 MS+Y F SG E+ YEGKVLLI NTASKCG TPQY +L+ +Y++ G++ +LG Sbjct: 1 MSVYDFKAKDISGEEISLSKYEGKVLLIVNTASKCGFTPQYKELEDIYKKLGNEKFEILG 60 Query: 61 FPCNQFAGQEPGTSEEAEEFCQINYGVTFPIFAKVDVNGPDASLLFQYLKGQQPGEGGSS 120 FPCNQFA QEPG S + + FC+INYGVTFP+F K+DV G +A LF+YL Q G G Sbjct: 61 FPCNQFANQEPGGSGDIKNFCEINYGVTFPLFEKIDVKGENAHPLFKYLASQAGGILG-K 119 Query: 121 DIQWNFTKFLVDRSGNVVARVEPKESPESMKEQIEALL 158 +I+WNFTKFL+D+ G+VV R P P +K++I L+ Sbjct: 120 EIKWNFTKFLIDKKGDVVDRFAPVTKPSKIKDKIVKLM 157 >YBR244w [O] COG0386 Glutathione peroxidase Length = 162 Score = 176 bits (446), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 82/157 (52%), Positives = 105/157 (66%) Query: 2 SIYSFAGVKPSGAEVPFKDYEGKVLLIANTASKCGLTPQYGDLQKLYEQYGDKGLVVLGF 61 S Y G F +GKV+LI N ASKCG TPQY +L++LY++Y DKG V+LGF Sbjct: 4 SFYDLECKDKKGESFKFDQLKGKVVLIVNVASKCGFTPQYKELEELYKKYQDKGFVILGF 63 Query: 62 PCNQFAGQEPGTSEEAEEFCQINYGVTFPIFAKVDVNGPDASLLFQYLKGQQPGEGGSSD 121 PCNQF QEPG+ E+ EFCQ+NYGVTFPI K+DVNG +A ++ YLK Q+ G G Sbjct: 64 PCNQFGKQEPGSDEQITEFCQLNYGVTFPIMKKIDVNGSNADSVYNYLKSQKAGLLGFKG 123 Query: 122 IQWNFTKFLVDRSGNVVARVEPKESPESMKEQIEALL 158 I+WNF KFLVD +G VV R P S+ ++I++LL Sbjct: 124 IKWNFEKFLVDSNGKVVQRFSSLTKPSSLDQEIQSLL 160 >CAC1549 [O] COG0386 Glutathione peroxidase Length = 181 Score = 174 bits (441), Expect = 5e-44, Method: Compositional matrix adjust. Identities = 90/182 (49%), Positives = 109/182 (59%), Gaps = 25/182 (13%) Query: 1 MSIYSFAGVKPSGAEVPFKDYEGKVLLIANTASKCGLTPQYGDLQKLYEQYGDKGLVVLG 60 MS+Y F G EV Y+GKVL+IANTASKCG TPQY L+KLY++Y DKGL +LG Sbjct: 1 MSVYDFKAKTIEGKEVSLDTYKGKVLIIANTASKCGFTPQYEGLEKLYKEYKDKGLEILG 60 Query: 61 FPCNQFAGQEPGTSEEAEEFCQINYGVTFPIFAKVDVNGPDASLLFQYLKGQQPGEG--- 117 FP NQFA QEPG + E + FC++NYGVTF +F K DV +A LF YL P +G Sbjct: 61 FPSNQFAEQEPGDNNEVKNFCKLNYGVTFQLFEKTDVRDENAHPLFNYLTENAPFKGFDL 120 Query: 118 ---------------------GSSDIQWNFTKFLVDRSGNVVARVEPKESPESMKEQIEA 156 G+S ++WNFTKFL+DR GNVV R EP P M + IE Sbjct: 121 NHPTGKMLSEVLEKNFPKFLEGNS-VKWNFTKFLIDRDGNVVKRFEPTSEPSDMVKDIEK 179 Query: 157 LL 158 LL Sbjct: 180 LL 181 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 12,402,066 Number of extensions: 515441 Number of successful extensions: 604 Number of sequences better than 1.0e-05: 18 Number of HSP's gapped: 591 Number of HSP's successfully gapped: 18 Length of query: 158 Length of database: 38,378,431 Length adjustment: 98 Effective length of query: 60 Effective length of database: 27,075,699 Effective search space: 1624541940 Effective search space used: 1624541940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 111 (47.4 bits)