BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0069.0007.p (546 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPy2085 [F] COG2759 Formyltetrahydrofolate synthetase 528 e-149 SPy1213 [F] COG2759 Formyltetrahydrofolate synthetase 512 e-145 STM1766 [T] COG3850 Signal transduction histidine kinase, nitrat... 480 e-135 YBR084w_2 [F] COG2759 Formyltetrahydrofolate synthetase 382 e-105 YGR204w_2 [F] COG2759 Formyltetrahydrofolate synthetase 368 e-101 >SPy2085 [F] COG2759 Formyltetrahydrofolate synthetase Length = 86 Score = 528 bits (1359), Expect = e-149, Method: Compositional matrix adjust. Identities = 0/547 (0%), Positives = 0/547 (0%), Gaps = 6/547 (1%) Query: 1 MRLITEVASAAGIHEEHLELYGKYKSKLSPSLWDELKHNQDGKLVLVTAMNPTPAGEGKT 60 E+A + G+ + L Sbjct: 14 DYDNLELACQHHLMLTLELKDGEKLQAKASDLVSRKNVEYLVVEAAGETRELRLDKITSF 73 Query: 61 LTTIGLSQALNAAGVKTVAALREPSLXXXXXXXXXXXXXXXXQIVPADEINLHFTGDIHA 120 Sbjct: 74 133 Query: 121 VTSAHNLLSAMIDNHLFQGNLLGLDPQRIVWKRVMDMNDRSLRSIVTGL-GDGNGTVRES 179 Sbjct: 134 193 Query: 180 GFQITTASEIMAVLCLCDGLADLKKRLSRMLVGYDQSGQPVTAKDIGAVEAMTALLKEAV 239 Sbjct: 194 253 Query: 240 KPNLVQTLEGTPVIVHGGPFANIAHGCSSVIGTRYALKLGDVVVTEAGFGADLGAEKFMD 299 Sbjct: 254 313 Query: 300 IKCRQXXXXXXXXXXXXXXKSLKYNGGVRKDELHQTNQAALLSGISNMERHIENLGK-FG 358 Sbjct: 314 373 Query: 359 VPVLVALNHFEGDAPSEINDVLEACRRLGVPAAVSRVWXXXXXXXXXXXXXXXXXXXQGE 418 Sbjct: 374 433 Query: 419 GQPAAFAPLYEDNLDIPAKIKKIVTEIYRGAEVAFSPAAKRSLGMIEKLGLHQLQVCMAK 478 Sbjct: 434 ---- 489 Query: 479 TPYSFSDQPRLLGAPEGFTMGVRDITLSLGAGFAVVITGNIVTMPGLPAQPAAEGIQVDL 538 Sbjct: 490 549 Query: 539 DGVINGL 545 Sbjct: 550 556 >SPy1213 [F] COG2759 Formyltetrahydrofolate synthetase Length = 86 Score = 512 bits (1319), Expect = e-145, Method: Compositional matrix adjust. Identities = 0/543 (0%), Positives = 0/543 (0%), Gaps = 4/543 (0%) Query: 4 ITEVASAAGIHEEHLELYGKYKSKLSPSLWDELKHNQDGKLVLVTAMNPTPAGEGKTLTT 63 + LEL K + S K+ + + + + + Sbjct: 16 DNLELACQHHLMLTLELKDGEKLQAKASDLVSRKNVEYLVVEAAGETRELRLDKITSFSH 75 Query: 64 IGLSQALNAAGVKTVAALREPSLXXXXXXXXXXXXXXXXQIVPADEINLHFTGDIHAVTS 123 Sbjct: 76 135 Query: 124 AHNLLSAMIDNHLFQGNLLGLDPQRIVWKRVMDMNDRSLRSIVTGLGDG-NGTVRESGFQ 182 Sbjct: 136 195 Query: 183 ITTASEIMAVLCLCDGLADLKKRLSRMLVGYDQSGQPVTAKDIGAVEAMTALLKEAVKPN 242 Sbjct: 196 255 Query: 243 LVQTLEGTPVIVHGGPFANIAHGCSSVIGTRYALKLGDVVVTEAGFGADLGAEKFMDIKC 302 Sbjct: 256 315 Query: 303 RQXXXXXXXXXXXXXXKSLKYNGGVRKDELHQTNQAALLSGISNMERHIENLGKFGVPVL 362 Sbjct: 316 375 Query: 363 VALNHFEGDAPSEINDVLEACRRLGVPAAVSRVWXXXXXXXXXXXXXXXXXXXQGEGQPA 422 Sbjct: 376 --- 432 Query: 423 AFAPLYEDNLDIPAKIKKIVTEIYRGAEVAFSPAAKRSLGMIEKLGLHQLQVCMAKTPYS 482 Sbjct: 433 492 Query: 483 FSDQPRLLGAPEGFTMGVRDITLSLGAGFAVVITGNIVTMPGLPAQPAAEGIQVDLDGVI 542 Sbjct: 493 552 Query: 543 NGL 545 Sbjct: 553 555 >STM1766 [T] COG3850 Signal transduction histidine kinase, nitrate/nitrite-specific Length = 598 Score = 480 bits (1235), Expect = e-135, Method: Compositional matrix adjust. Identities = 35/546 (6%), Positives = 103/546 (18%), Gaps = 6/546 (1%) Query: 1 MRLITEVASAAGIHEEHLELYGKYKSKLSPSLWDELKHNQDGKLVLVTAMNPTPAGEGKT 60 M + + ++ L G + + Sbjct: 1 MFKRCFSPLTLVNQLALIVMLSTAIGVAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRL 60 Query: 61 LTTIGLSQALNAAGVKTVAALREPSLXXXXXXXXXXXXXXXXQIVPADEINLHFTGDIHA 120 L + L + P L Q +E++ +A Sbjct: 61 LAAVPLDAKDQKLLDEMEQTAFSPELTRAAERDGQQKQLKALQDYWHNELSPGLQHAQNA 120 Query: 121 VTSAHNLLSAMIDNHLFQGNLLGLDPQRIVWKRVMDMNDRSLRSIVTGLG-DGNGTVRES 179 A ++ + + RI ++ +++ Sbjct: 121 HAVAEDVTRFVAGLDRLVTSFDHTTELRIERVVLVHRVMAIFMALLLVFTIIWLRVRLLQ 180 Query: 180 GFQITTASEIMAVLCLCDGLADLKKRLSRMLVGYDQSGQPVTAKDIGAVEAMTALLKEAV 239 ++ + A++ R +G + + AV K A Sbjct: 181 PWKQLLSMARAVSQRDFTQRANISGRNEMAALGSALNNMSEELAESYAVLEQRVQEKTAG 240 Query: 240 KPNLVQTLEGTPVIVHGGPFANIAHGCSSVIGTRYALKLGDVVVTEAGFGADLGAEKFMD 299 + Q L S + + + + Sbjct: 241 LEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQNLTQLHDIELRVYDLEDEDNHQEF 300 Query: 300 IKCRQXXXXXXXXXXXXXXKSLKYNGGVRKDELHQTNQAALLSGISNMERHIENLGKFGV 359 NGG + ++ + Sbjct: 301 TCQSDISCDDKGCHLCPRSALPMINGGTTLKWRLTDSHTQYGILLATLPYGRHLSHDQQQ 360 Query: 360 PVLVALNHFEGDAPSEINDVLEACRRLGVPAAVSRVWXXXXXXXXXXXXXXXXXXXQGEG 419 V + + + + + A Q + Sbjct: 361 LVDTLVEQLTATLALDRHQERQQQLIVMEERATIARELHDSIAQSLSCMKMQVSCLQMQ- 419 Query: 420 QPAAFAPLYEDNLDIPAKIKKIVTEIYRGAEVAFSPAAKRSLGMIEKLGLHQLQVCMAKT 479 L E + ++ ++I+ + + + + + L + Sbjct: 420 -GDV---LPESSRELLSQIRNELNCSWAQLRELLTTFRLQLTEPGLRPALEASCQEFSAR 475 Query: 480 PYSFSDQPRLLGAPEGFTMGVRDITLSLGAGFAVVITGNIVTMPGLPAQPAAEGIQVDLD 539 L + + + + + + + +++ + Sbjct: 476 FGFTVKLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSHADDVVVTVTQCGKQVKLKVQ 535 Query: 540 GVINGL 545 G+ Sbjct: 536 DNGCGV 541 >YBR084w_2 [F] COG2759 Formyltetrahydrofolate synthetase Length = 86 Score = 382 bits (980), Expect = e-105, Method: Compositional matrix adjust. Identities = 0/620 (0%), Positives = 0/620 (0%), Gaps = 82/620 (13%) Query: 4 ITEVASAAGIHEEHLELYGKYKSKLSPSLWDELKHNQDGKLVLVTAMNPTPAGEGKTLTT 63 + A H L L K KL D + LV+ A K + Sbjct: 14 DYDNLELACQHHLMLTLELKDGEKLQAKASDLVSRKNVEYLVVEAAGETRELRLDKITSF 73 Query: 64 IGLSQALNAA-GVKTVAALREPSLXXXXXXXXXXXXXXXXQIVPADEINLHFTGDIHAVT 122 Sbjct: 74 SHPEIGTVVVS 133 Query: 123 SAHNLLSAMIDNHLF-----------------------------QGNLLGLD-------- 145 Sbjct: 134 193 Query: 146 ---------------PQRIVWKRVMDMNDRSLRSIVTG-LGDGNGTVRESGFQITTASEI 189 Sbjct: 194 253 Query: 190 MAVLCLCDGLADLKKRLSRMLVGYDQSGQPVTAKDIGAVEAMTALLKEAVKPNLVQTLEG 249 Sbjct: 254 313 Query: 250 TPVIVHGGPFANIAHGCSSVIGTRYALKL---------------GDVVVTEAGFGADLGA 294 Sbjct: 314 373 Query: 295 EKFMDIKCRQXXXXXXXXXXXXXXKSLKYNGGVRK--------DELHQTNQAALLSGISN 346 Sbjct: 374 433 Query: 347 MERHIENLGKFGVPVLVALNHFEGDAPSEINDVLEACRRLGVPAAV-SRVWXXXXXXXXX 405 Sbjct: 434 ---- 489 Query: 406 XXXXXXXXXXQGEGQPAAFAPLYEDNLDIPAKIKKIVTEIYRGAEVAFSPAAKRSLGMIE 465 Sbjct: 490 549 Query: 466 KLGLHQLQVCMAKTPYSFSDQPRLLGAPEGFTMGVRDITLSLGAGFAVVITGNIVTMPGL 525 Sbjct: 550 609 Query: 526 PAQPAAEGIQVDLDGVINGL 545 Sbjct: 610 629 >YGR204w_2 [F] COG2759 Formyltetrahydrofolate synthetase Length = 86 Score = 368 bits (944), Expect = e-101, Method: Compositional matrix adjust. Identities = 0/618 (0%), Positives = 0/618 (0%), Gaps = 79/618 (12%) Query: 2 RLITEVASAAGIHEEHLELYGKYKSKLSPSLWDELKHNQDGKLVLVTAMNPTPAGEGKTL 61 + A H L L K KL D + LV+ A K Sbjct: 12 CDDYDNLELACQHHLMLTLELKDGEKLQAKASDLVSRKNVEYLVVEAAGETRELRLDKIT 71 Query: 62 TTIGLSQALNAA-GVKTVAALREPSLXXXXXXXXXXXXXXXXQIVPADEINLHFTGDIHA 120 + Sbjct: 72 SFSHPEIGTVVVS 131 Query: 121 VTSAHNLLSAMIDNHL-----------FQGNL---------------------------- 141 Sbjct: 132 191 Query: 142 -------------LGLDPQRIVWKRVMDMNDRSLRSIVTGLG--DGNGTVRESGFQITTA 186 Sbjct: 192 - 250 Query: 187 SEIMAVLCLCDGLADLKKRLSRMLVGYDQSGQPVTAKDIGAVEAMTALLKEAVKPNLVQT 246 Sbjct: 251 310 Query: 247 LEGTPVIVHGGPFANIAHGCSSVIGTRYALKL----------GDVVVTEAGFGADLGAEK 296 Sbjct: 311 370 Query: 297 FMDIKCRQXXXXXXXXXXXXXXKSLKYNGGVRK--------DELHQTNQAALLSGISNME 348 Sbjct: 371 430 Query: 349 RHIENLGKFGVPVLVALNHFEGDAPSEINDVLEACRRLGVPAAV-SRVWXXXXXXXXXXX 407 Sbjct: 431 ---- 486 Query: 408 XXXXXXXXQGEGQPAAFAPLYEDNLDIPAKIKKIVTEIYRGAEVAFSPAAKRSLGMIEKL 467 Sbjct: 487 546 Query: 468 GLHQLQVCMAKTPYSFSDQPRLLGAPEGFTMGVRDITLSLGAGFAVVITGNIVTMPGLPA 527 Sbjct: 547 606 Query: 528 QPAAEGIQVDLDGVINGL 545 Sbjct: 607 624 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 37,654,239 Number of extensions: 1522209 Number of successful extensions: 3401 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 3369 Number of HSP's successfully gapped: 11 Length of query: 546 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 435 Effective length of database: 25,576,357 Effective search space: 11125715295 Effective search space used: 11125715295 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)