BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0069.0005.p (275 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yrhG [P] COG2116 Formate/nitrite family of transporters 289 4e-78 lin0912 [P] COG2116 Formate/nitrite family of transporters 281 7e-76 VC1695 [P] COG2116 Formate/nitrite family of transporters 122 8e-28 VCA0540_1 [P] COG2116 Formate/nitrite family of transporters 113 4e-25 YPO1384 [P] COG2116 Formate/nitrite family of transporters 111 1e-24 >BS_yrhG [P] COG2116 Formate/nitrite family of transporters Length = 266 Score = 289 bits (739), Expect = 4e-78, Method: Compositional matrix adjust. Identities = 144/255 (56%), Positives = 182/255 (71%) Query: 1 MAYNKPQGIAEVTVENGIKKAHNPLSTVXXXXXXXXXXXXXXXXXDIRVIAGAPKEWGSI 60 MA+ KP IAE +E G+KK PL ++ DIRVI PKEWGS+ Sbjct: 1 MAFRKPDEIAEAAIEAGMKKIKLPLPSLLVLGFLGGAFIALGYLLDIRVIGDLPKEWGSL 60 Query: 61 ANFIGASVFPVGLIMVLLAGGELLTGNMMAVPLAFMSKKISFWETIKNLVLITISNFAGA 120 ++ IGA+VFPVGLI+V+LAG EL+TGNMM+V +A S+KIS E N ++TI N GA Sbjct: 61 SSLIGAAVFPVGLILVVLAGAELITGNMMSVAMALFSRKISVKELAINWGIVTIMNLIGA 120 Query: 121 LFVAYFFGHVVGLTADGVYLDKLVDMAGHKIDATFLQAFVSGIGCNWLVALAVWLSYGAD 180 LFVAYFFGH+VGLT G YL+K + +A K+D +F + +S IGCNWLV LAVWLS+GA Sbjct: 121 LFVAYFFGHLVGLTETGPYLEKTIAVAQGKLDMSFGKVLISAIGCNWLVCLAVWLSFGAQ 180 Query: 181 NFSGKILGIWFPTMAFVAIGFQHVVANMFLIPAAIFEGHFTWSQYFSNFVPVWLGNLTGG 240 + +GKILGIWFP MAFVAIGFQHVVANMF+IPAAIF G FTW Q+ N +P ++GN+ GG Sbjct: 181 DAAGKILGIWFPIMAFVAIGFQHVVANMFVIPAAIFAGSFTWGQFIGNIIPAFIGNVIGG 240 Query: 241 AIFVAFAYWMAYLRK 255 A+FV Y++AY +K Sbjct: 241 AVFVGLIYFIAYHKK 255 >lin0912 [P] COG2116 Formate/nitrite family of transporters Length = 270 Score = 281 bits (719), Expect = 7e-76, Method: Compositional matrix adjust. Identities = 142/269 (52%), Positives = 185/269 (68%), Gaps = 6/269 (2%) Query: 1 MAYNKPQGIAEVTVENGIKKAHNPLSTVXXXXXXXXXXXXXXXXXDIRVIAGAPKEWGSI 60 M Y PQ + E+T+E G +KA++ T+ IR + P EWGS Sbjct: 1 MGYYSPQEVTEITIEKGTQKANSSTLTLVLLGFLGGAFISLGYLLYIRAVGTMPHEWGSF 60 Query: 61 ANFIGASVFPVGLIMVLLAGGELLTGNMMAVPLAFMSKKISFWETIKNLVLITISNFAGA 120 A IGAS+FPVGL+ +LL GGEL+TGNMMAV +A+ KKISF + ++N ++++ N GA Sbjct: 61 ATLIGASLFPVGLVCILLGGGELITGNMMAVAIAWYDKKISFQQLLRNWAIVSVMNLVGA 120 Query: 121 LFVAYFFGHVVGLTADGVYLDKLVDMAGHKIDATFLQAFVSGIGCNWLVALAVWLSYGAD 180 FVAYFFGH VGLT +G +L K + AG KI+ F AFVSGIGCNW V +AVWL Y A Sbjct: 121 FFVAYFFGHFVGLT-EGDFLPKTLATAGAKINDPFWVAFVSGIGCNWFVGIAVWLCYAAK 179 Query: 181 NFSGKILGIWFPTMAFVAIGFQHVVANMFLIPAAIFEGHFTWSQYFSNFVPVWLGNLTGG 240 +F+GKILGIWFP MAFVAIGFQHVVANMF+IPAAIF G+++W+ + N +PV+LGN+ GG Sbjct: 180 DFAGKILGIWFPVMAFVAIGFQHVVANMFIIPAAIFAGYYSWADFIWNVIPVYLGNVVGG 239 Query: 241 AIFVAFAYWMAYLRKAPAAV-----QPVE 264 A+FV+ Y++AY + AP V QP+E Sbjct: 240 AVFVSLFYFLAYKKNAPKKVKEEIHQPIE 268 >VC1695 [P] COG2116 Formate/nitrite family of transporters Length = 280 Score = 122 bits (305), Expect = 8e-28, Method: Compositional matrix adjust. Identities = 76/228 (33%), Positives = 121/228 (53%), Gaps = 22/228 (9%) Query: 49 VIAGAPKEWGSIANFIGASVFPVGLIMVLLAGGELLTGNMMAVPLAFMSKKISFWETIKN 108 V GA + +G F +GLI+V++ GGEL T +++ + +A S KIS+ E ++N Sbjct: 54 VTTGAHDMPYGVTKLLGGLAFSLGLILVVITGGELFTSSVL-ILVAKASGKISWKELVRN 112 Query: 109 LVLITISNFAGALFVAYFFGHVVGLTADGVYLD-KLVDMAGHKIDATFLQAFVSGIGCNW 167 ++ N G++ + + DG L + ++ HK+ TFLQAF G+ CN Sbjct: 113 WTVVYFGNLCGSIILVFIMLATRQFMEDGGQLGLNAMAISQHKLHHTFLQAFALGLMCNI 172 Query: 168 LVALAVWLSYGADNFSGKILGIWFPTMAFVAIGFQHVVANMFLIPAAIFEGHFT----W- 222 LV LAVW+++ A + + K++ + P FV+ GF+H +ANMF +P AI +F W Sbjct: 173 LVCLAVWMTFSARSLTDKVMVLILPVAMFVSSGFEHCIANMFQVPMAIGIKYFAPESFWA 232 Query: 223 ------SQY---------FSNFVPVWLGNLTGGAIFVAFAYWMAYLRK 255 +QY +N +PV LGN+ GG +FV YW+ YL+ Sbjct: 233 MTGANIAQYADLNFVNFIVNNLIPVTLGNIVGGGVFVGMWYWLIYLKD 280 >VCA0540_1 [P] COG2116 Formate/nitrite family of transporters Length = 284 Score = 113 bits (282), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 78/229 (34%), Positives = 122/229 (53%), Gaps = 35/229 (15%) Query: 53 APKEWGSIANFIGASVFPVGLIMVLLAGGELLTGNMMAVPLAFMSKKISFWETIKNLVLI 112 A WG ++ G F +GLI++++ GGEL T ++++ +++ +++ISF + + + Sbjct: 62 ASAGWG-LSRLAGGVAFSMGLILIVICGGELFTSSVLS-SISWANREISFGKMLSIWGKV 119 Query: 113 TISNFAGALFVAYFFGHVVGLTADGVY-LDK------LVDMAGHKIDATFLQAFVSGIGC 165 + NF GA+F+ +TA G+Y LD+ +++A HK+ T +QAF GI C Sbjct: 120 YVGNFIGAIFLLLL------VTAAGLYQLDEGQWGLNALNIAQHKLHHTTVQAFALGILC 173 Query: 166 NWLVALAVWLSYGADNFSGKILGIWFPTMAFVAIGFQHVVANMFLIPAAIFEGHF----- 220 N LV LA+WL++ + N K P FV+ GF+H VANMF++P I +F Sbjct: 174 NLLVCLAIWLTFSSANAMTKAAMTIMPVAMFVSSGFEHCVANMFMVPLGIVIQNFAPDSF 233 Query: 221 ------TWSQY---------FSNFVPVWLGNLTGGAIFVAFAYWMAYLR 254 T SQY +N +PV LGN+ GGA+ V A W Y R Sbjct: 234 WQQVGVTASQYSDLNVTQFITANLIPVTLGNIVGGAVLVGLANWSIYRR 282 >YPO1384 [P] COG2116 Formate/nitrite family of transporters Length = 285 Score = 111 bits (277), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 83/273 (30%), Positives = 129/273 (47%), Gaps = 28/273 (10%) Query: 6 PQGIAEVTVENGIKKA-HNPLSTVXXXXXXXXXXXXXXXXXDIRVIAGAPKEWGSIANFI 64 P +A+V + G+ KA +P++T A +G A + Sbjct: 12 PSAMAKVAEDAGVYKATKHPMTTFYLAITAGVFISIAFVFYITATTGTAGVPFG-FAKLV 70 Query: 65 GASVFPVGLIMVLLAGGELLTGNMMAVPLAFMSKKISFWETIKNLVLITISNFAGALF-- 122 G F +GL++V++ GG+L T ++ +A S +I++ + N V + I N GALF Sbjct: 71 GGICFSLGLMLVVVCGGDLFTSTVLTT-VAKASGRITWRQLATNWVNVYIGNLFGALFFV 129 Query: 123 -VAYFFGHVVGLTADGVYLDKLVDMAGHKIDATFLQAFVSGIGCNWLVALAVWLSYGADN 181 + +F G A+G + ++ A HK+ TF++A GI N +V LAVW+SY Sbjct: 130 GLIWFAGQYT--VANGQWGLNVLQTAEHKLHHTFIEAVCLGILANLMVCLAVWMSYSGRT 187 Query: 182 FSGKILGIWFPTMAFVAIGFQHVVANMFLIPAAIFE-------------------GHFTW 222 + K+L + P FVA GF+H +ANMF+IP I H T Sbjct: 188 ITDKMLAMILPVGMFVASGFEHSIANMFMIPLGIVIKNFASPEFWQAIGSAPEQFAHLTV 247 Query: 223 SQY-FSNFVPVWLGNLTGGAIFVAFAYWMAYLR 254 S + N +PV +GN+ GG + V YW+ YLR Sbjct: 248 SNFIIDNLIPVTIGNIIGGGLLVGLTYWVIYLR 280 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.326 0.140 0.442 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,357,684 Number of extensions: 825008 Number of successful extensions: 3544 Number of sequences better than 1.0e-05: 24 Number of HSP's gapped: 3477 Number of HSP's successfully gapped: 24 Length of query: 275 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 170 Effective length of database: 26,268,361 Effective search space: 4465621370 Effective search space used: 4465621370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 115 (48.9 bits)