BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0069.0004.p (701 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yoaE [C] COG0243 Anaerobic dehydrogenases, typically selenocy... 755 0.0 RSc2859 [C] COG0243 Anaerobic dehydrogenases, typically selenocy... 514 e-145 BH2853 [C] COG0243 Anaerobic dehydrogenases, typically selenocys... 501 e-141 PAB0532 [C] COG0243 Anaerobic dehydrogenases, typically selenocy... 363 e-100 PH1359 [C] COG0243 Anaerobic dehydrogenases, typically selenocys... 357 3e-98 >BS_yoaE [C] COG0243 Anaerobic dehydrogenases, typically selenocysteine-containing Length = 289 Score = 755 bits (1950), Expect = 0.0, Method: Compositional matrix adjust. Identities = 0/674 (0%), Positives = 0/674 (0%), Gaps = 11/674 (1%) Query: 6 DGVFPAVCPLDCPDTCGLLVHKQNGKIIKVEGNPEHPVTKGAICNKVRHMAERVHHPERL 65 D + LD P + + K ++ + + RL Sbjct: 9 DLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLSKSAVALRL 68 Query: 66 LQPLRRTGPKGSGSFTPISWEEAIGEITGRFKELSADYGPESILPYSFYGNMGVLSVDGM 125 + ++ A + + L S +G ++ Sbjct: 69 TARPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRSPEWSAFGIRPPLRLGEFTMPEH 128 Query: 126 DRRFFNALGASRLEQSICNAAGSAGWKYTMGFNGGTSPEDTVNADLIIVWGGNIVSTSMH 185 + QS + + V G + Sbjct: 129 KFVTLEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFLGNAPTIPPVLYGLNETRPSQ 188 Query: 186 QVVYAEQARKRGAKVVVIDVHRNRTAQWADWFLPLYPGTDTALALGLMHILFRDGMTDDA 245 ++ A T Y + Sbjct: 189 DKDDEQEVFYTTALAQDQADGYVLTGHPVMLQGGEYVMFTYEGLGTGVQEFILTVYGTCM 248 Query: 246 FLERYTIGHEELRSHVLAYPPERVAEITGIAAADIEKLAKLYGEAKVSHIHIGNGLQHHD 305 Sbjct: 249 308 Query: 306 NGGMNVRTIACLPALTGAWLKTXXXXXXXXXXYSSTNDRALTRPDLXXXXXXXXXXXXXX 365 Sbjct: 309 368 Query: 366 GEALELKEQPVMGMFVYCSNPVVVAPDAERVRKGFAREDLFTVVHDLFMTDTALYADIVL 425 Sbjct: 369 428 Query: 426 PATSTFENVDLYTSYWHHYIQLQEPVLDAPGECKSNVEVFSLLGKAMGLDAEAFSESPYD 485 Sbjct: 429 488 Query: 486 MIRQALDYPRNPYLNGVTLERLTAERFVKLDMSPQADYLDNLPTPSGKIELYSKAMELAG 545 Sbjct: 489 - 547 Query: 546 LPPLPTYTPLKEGYDGQQRPQPGTAYPLMFVSPPNHNFLNSTFSNVEKHQRMEKRPTLQI 605 Sbjct: 548 ---------- 597 Query: 606 HPEDAAVRGIGEGDGLTVYNGRGSIELWAMVTDKMLPGTVISQGLWWEGKGHKQRVNVLT 665 Sbjct: 598 657 Query: 666 PDRLADMGNGATFF 679 Sbjct: 658 671 >RSc2859 [C] COG0243 Anaerobic dehydrogenases, typically selenocysteine-containing Length = 289 Score = 514 bits (1324), Expect = e-145, Method: Compositional matrix adjust. Identities = 0/694 (0%), Positives = 0/694 (0%), Gaps = 23/694 (3%) Query: 8 VFPAVCPLDCPDTCGLLVHKQNGKIIKVEGNPEHPVTKGAICNKVRHMAERVHHPERLLQ 67 + + N + R + Sbjct: 5 GIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLSKSAV 64 Query: 68 PLRRTGPKGSGSFTPISWEEAIGEITGRFKELSADYGPESILPYSFYGNMGVLSVDGMDR 127 LR T ++ K+ + + + + Sbjct: 65 ALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQ-TPALYRRSPEWSAFGIRPPLRLGEF 123 Query: 128 RFFNALGASRLEQSICNAAGSAGWKYTMGFNGGTSPEDTVNADLIIVWGGNIVSTSMHQV 187 + + + S + D + Sbjct: 124 TMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFLGNAPTIPPVLYGLNE 183 Query: 188 VYAEQARKRGAKVVVIDVHRNRTAQWADWFLPLYPGTDTALALGLMHILFRDGMTDDAFL 247 Q + +V A + L + Sbjct: 184 TRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVMLQGGEYVMFTYEGLGTGVQEFILTV 243 Query: 248 ERYTIGHEELRSHVLAYPPERVAEITGIAAADIEKLAKLYGEAKVSH-----IHIGNGLQ 302 Sbjct: 244 303 Query: 303 HHDNGGMNVRTIACLPALTGAWLKTXXXXXXXXXXYSSTNDRALTRPDLXXX--XXXXXX 360 Sbjct: 304 363 Query: 361 XXXXXGEALELKEQPVMG-----MFVYCSNPVVVAPDAERVRKGFAREDLFTVVHDLFMT 415 Sbjct: 364 423 Query: 416 DTALYADIVLPATSTFENVDLYTSYWHHYIQLQEPVLDAPGECKSNVEVFSLLGKAMGLD 475 Sbjct: 424 483 Query: 476 AEAFSESPYDMIRQALDY--PRNPYLNGVTLERLTAERFVKLDMSPQADYL-DNLPTPSG 532 Sbjct: 484 ---- 539 Query: 533 KIELYSKAMELAGLPPLPTYTPLKEGYDGQQRPQPGTAYPLMFVSPPNHNFLNSTFSNVE 592 Sbjct: 540 -- 597 Query: 593 KHQRMEKRPTLQIHPEDAAVRGIGEGDGLTVYNGRGSIELWAMVTDKMLPGTVISQGLWW 652 Sbjct: 598 657 Query: 653 EGKG-HKQRVNVLTPDRLADMGNGATFFSTTVEV 685 Sbjct: 658 691 >BH2853 [C] COG0243 Anaerobic dehydrogenases, typically selenocysteine-containing Length = 289 Score = 501 bits (1289), Expect = e-141, Method: Compositional matrix adjust. Identities = 0/631 (0%), Positives = 0/631 (0%), Gaps = 24/631 (3%) Query: 65 LLQPLRRTGPKGSGSFTPISWEEAIGEITGRFKELSADYGPESILPYSFYGNMGVLSVDG 124 + Q + G Sbjct: 1 MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLS 60 Query: 125 MDRRFFNALGASRLEQSICNAAGSAGWKYTMGFNGGTSPEDTVNADLIIVWGGNIVSTSM 184 L+ ++ S G + Sbjct: 61 KSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRSPEWSAFGIRPPLRL 120 Query: 185 HQVVYAEQARKRGAKVVVIDVHRNRTAQWADWFLPLYPGTDTALALGLMHILFRDGMTDD 244 + E +I V ++ + + L + + Sbjct: 121 GEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFLGNAPTIPPVLYG 180 Query: 245 AFLERYTIGHEELRSHVLAYPPERVAEITGIAAADIEKLAKLYGEAKVSHIHIGNGLQHH 304 R + ++ + + + L + Sbjct: 181 LNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVMLQGGEYVMFTYEGLGTGVQEFI 240 Query: 305 DNGGMNVRTIACLPALTGAWLKTXXXXXXXXXXYSSTNDRALTRPDLXXXXXXXXXXXXX 364 + L G ++ N R + Sbjct: 241 LTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRPINLRCELLIPI- 299 Query: 365 XGEALELKEQPVMGMFVYCSNPVVVAPDAERVRKGFAREDLFTVVHDLFMTDTALYADIV 424 Sbjct: 300 359 Query: 425 LPATSTFENVDLYTSYWHHYIQLQEPVLDAPGECKSNVEVFSLLGKAMGLDAEAFSESPY 484 Sbjct: 360 419 Query: 485 DMIRQALDYPRNPYLNGVTLERLTAE--RFVKLDMSPQAD---YLDNLPTPSGKIELYSK 539 Sbjct: 420 - 478 Query: 540 AMELAGLPPLPTYTPLKEGYDGQQRPQPGTAYPLMFVSPPNHNFLNSTFSNVEKHQRMEK 599 Sbjct: 479 ---------------- 522 Query: 600 RPTLQIHPEDAAVRGIGEGDGLTVYNGRGSIELWAMVTDKMLPGTVISQGLWWEGKGHKQ 659 Sbjct: 523 582 Query: 660 -RVNVLTPDRLADMGNGATFFSTTVEVKRRE 689 Sbjct: 583 613 >PAB0532 [C] COG0243 Anaerobic dehydrogenases, typically selenocysteine-containing Length = 289 Score = 363 bits (931), Expect = e-100, Method: Compositional matrix adjust. Identities = 0/678 (0%), Positives = 0/678 (0%), Gaps = 51/678 (7%) Query: 11 AVCPLDCPDTCGLLVHKQNGKIIKVEGNPEHPVTKGAICNKVRHMAERVHHPERLLQPLR 70 + Q + + R + + Sbjct: 3 QAGIIRDLLIWLEGHLDQPLSLD-NVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLSK 61 Query: 71 RTGPKGSGSFTPISWEEAIGEITGRFKELSADYGPESILPYSFYGNMGVLSVDGMDRRFF 130 I + + + + Sbjct: 62 SAVAL----RLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRSPEW-SAFGIRP 116 Query: 131 NALGASRLEQSICNAAGSAGWKYTMGFNGGTSPEDTVNADLIIVWGGNIVSTSMHQVVYA 190 + + S E + + + + Sbjct: 117 PLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFLGNAPTIPP 176 Query: 191 EQARKRGAKVVVIDVHRNRTAQWADWFLPLYPGTDTALALGLMHILFRDGMTDDAFLERY 250 G ++ + D + G +L + Sbjct: 177 ---VLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVMLQGGEYVMFTYEGLG 233 Query: 251 TIGHEELRSHVLAYPPERVAEITGIAAADIEKLAKLYGEAKVSHIHIGNGLQHHDNGGMN 310 T E + + P + A+ Sbjct: 234 TGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGD- 292 Query: 311 VRTIACLPALTGAWLKTXXXXXXXXXXYSSTNDRALTRPDLXXXXXXXXXXXXXXGEALE 370 Sbjct: 293 -------- 344 Query: 371 LKEQPVMGMFVYCSNPVVVAPDAERVRKGFAREDLFTVVHDLFMTDTALYADIVLPATST 430 Sbjct: 345 -- 402 Query: 431 FENVDLYTSYWHHYIQLQEPVLDAPGECKSNVEVFSLLGKAMGLDAEAFSESPYDMIRQA 490 Sbjct: 403 -- 460 Query: 491 LDYPRNPYLNGVTLERLTAERFVKLDMSPQADYLDNLPTPSGKIELYSKAMELAGLPPLP 550 Sbjct: 461 ----------- 509 Query: 551 TYTPLKEGYDGQQRPQPGTAYPLMFVSPPNHNFLNSTFSNVEKHQRMEKRPTLQIHPEDA 610 Sbjct: 510 ---------------- 553 Query: 611 AVRGIGEGDGLTVYNGRGSIELWAMVTDKMLPGTVISQGLWWEGKGHKQRVNVLTPD-RL 669 Sbjct: 554 - 612 Query: 670 ADMGNGATFFSTTVEVKR 687 Sbjct: 613 630 >PH1359 [C] COG0243 Anaerobic dehydrogenases, typically selenocysteine-containing Length = 289 Score = 357 bits (917), Expect = 3e-98, Method: Compositional matrix adjust. Identities = 0/676 (0%), Positives = 0/676 (0%), Gaps = 47/676 (6%) Query: 11 AVCPLDCPDTCGLLVHKQNGKIIKVEGNPEHPVTKGAICNKVRHMAERVHHPERLLQPLR 70 + L L + + + + H RL + Sbjct: 6 IIRDLLIWLEGHLDQPLSLDNVA-AKAGYSKWHLQRMFKDVTGHAIGAYIRARRLSKSAV 64 Query: 71 RTGPKGSGSFTPISWEEAIGEITGRFKELSADYGPESILPYSFYGNMGVLSVDGMDRRFF 130 + T ++ S + + + F Sbjct: 65 ALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRSPEWSAFGIRP-PLRLGEF 123 Query: 131 NALGASRLEQSICNAAGSAGWKYTMGFNGGTSPEDTVNADLIIVWGGNIVSTSMHQVVYA 190 + G + G + + Sbjct: 124 TMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFLGNAPTIPPVLYGLN- 182 Query: 191 EQARKRGAKVVVIDVHRNRTAQWADWFLPLYPGTDTALALGLMHILFRDGMTDDAFLERY 250 + TA D + G +++F E Sbjct: 183 --ETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVMLQGGEYVMFTYEGLGTGVQEFI 240 Query: 251 TIGHEELRSHVLAYPPERVAEITGIAAADIEKLAKLYGEAKVSHIHIGNGLQHHDNGGMN 310 + + + A D + + Sbjct: 241 LTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRPINL- 299 Query: 311 VRTIACLPALTGAWLKTXXXXXXXXXXYSSTNDRALTRPDLXXXXXXXXXXXXXXGEALE 370 Sbjct: 300 ---------- 349 Query: 371 LKEQPVMGMFVYCSNPVVVAPDAERVRKGFAREDLFTVVHDLFMTDTALYADIVLPATST 430 Sbjct: 350 409 Query: 431 FENVDLYTSYWHHYIQLQEPVLDAPGECKSNVEVFSLLGKAMGLDAEAFSESPYDMIRQA 490 Sbjct: 410 -- 467 Query: 491 LDYPRNPYLNGVTLERLTAERFVKLDMSPQADYLDNLPTPSGKIELYSKAMELAGLPPLP 550 Sbjct: 468 ----------- 516 Query: 551 TYTPLKEGYDGQQRPQPGTAYPLMFVSPPNHNFLNSTFSNVEKHQRMEKRPTLQIHPEDA 610 Sbjct: 517 ---------------- 560 Query: 611 AVRGIGEGDGLTVYNGRGSIELWAMVTDKMLPGTVISQGLWWEGKGHKQRVNVLTPD-RL 669 Sbjct: 561 - 619 Query: 670 ADMGNGATFFSTTVEV 685 Sbjct: 620 635 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 54,789,206 Number of extensions: 2529069 Number of successful extensions: 6094 Number of sequences better than 1.0e-05: 131 Number of HSP's gapped: 5745 Number of HSP's successfully gapped: 191 Length of query: 701 Length of database: 38,378,431 Length adjustment: 113 Effective length of query: 588 Effective length of database: 25,345,689 Effective search space: 14903265132 Effective search space used: 14903265132 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 119 (50.4 bits)