BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0064.0001.p (460 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_murA [M] COG0766 UDP-N-acetylglucosamine enolpyruvyl transferase 539 e-153 BH3749 [M] COG0766 UDP-N-acetylglucosamine enolpyruvyl transferase 536 e-152 lin2670 [M] COG0766 UDP-N-acetylglucosamine enolpyruvyl transferase 483 e-136 SA1902 [M] COG0766 UDP-N-acetylglucosamine enolpyruvyl transferase 473 e-133 SP1966 [M] COG0766 UDP-N-acetylglucosamine enolpyruvyl transferase 411 e-114 >BS_murA [M] COG0766 UDP-N-acetylglucosamine enolpyruvyl transferase Length = 436 Score = 539 bits (1388), Expect = e-153, Method: Compositional matrix adjust. Identities = 265/422 (62%), Positives = 322/422 (76%) Query: 1 MSKFIVRGGNRLTGSVKVSGAKNSVLPIIAASLLAEEGVSVIVDAPPLDDVMTISKVLES 60 M K IVRGG +L G+VKV GAKN+VLP+IAASLLA E SVI D P L DV TI++VL Sbjct: 1 MEKIIVRGGQKLNGTVKVEGAKNAVLPVIAASLLASEEKSVICDVPTLSDVYTINEVLRH 60 Query: 61 LGAGVTYQNDVIEVDARNITSYEAPYEWVRKMRASFLVMGPLLSRLGHTRISLPGGCAIG 120 LGA V ++N+ + V+A EAP+E+VRKMRAS LVMGPLL+R GH R++LPGGCAIG Sbjct: 61 LGADVHFENNEVTVNASYALQTEAPFEYVRKMRASVLVMGPLLARTGHARVALPGGCAIG 120 Query: 121 TRPIDQHLKGFEALGAEISLGQGYIEAKSNGRLRGAKVYLDVASVGATENIMMAAALAEG 180 +RPIDQHLKGFEA+GAEI +G G+IEA+ GRL+GAK+YLD SVGATEN++MAAALAEG Sbjct: 121 SRPIDQHLKGFEAMGAEIKVGNGFIEAEVKGRLQGAKIYLDFPSVGATENLIMAAALAEG 180 Query: 181 TTVIENAAKEPEIVDLANYLNXXXXXXXXXXXXXIRIEGVERLHGVKHHVIPDRIEAGTY 240 TT +EN AKEPEIVDLANY+N I+IEGVE+LHGVKHH+IPDRIEAGT+ Sbjct: 181 TTTLENVAKEPEIVDLANYINGMGGKIRGAGTGTIKIEGVEKLHGVKHHIIPDRIEAGTF 240 Query: 241 MAAAAITGGDVYVEGAIADHLGPVIAKMEEMGVTIIPDENGVRVISDKPLKAVDLKTLPY 300 M AAAIT G+V V+GA+ +HL +IAKMEEMGVTI + G+RVI K LK +D+KT+P+ Sbjct: 241 MVAAAITEGNVLVKGAVPEHLTSLIAKMEEMGVTIKDEGEGLRVIGPKELKPIDIKTMPH 300 Query: 301 PGFPTDMQSQMMALLLRSEGTSVVTETVFENRFMHVDEFHNMNAEIKIEGRSAIVTGNAA 360 PGFPTDMQSQMMALLLR+ GTS++TETVFENRFMH +EF MN +IKIEGRS I+ G Sbjct: 301 PGFPTDMQSQMMALLLRASGTSMITETVFENRFMHAEEFRRMNGDIKIEGRSVIINGPVQ 360 Query: 361 LVGAKVCATDLRXXXXXXXXXXXXXXTTEVSGTHHIDRGYVNLAEKLSGLGADIWRISME 420 L GA+V ATDLR T V+ H+DRGYV+ +KL+ LGADI R++ E Sbjct: 361 LQGAEVAATDLRAGAALILAGLVAEGHTRVTELKHLDRGYVDFHQKLAALGADIERVNDE 420 Query: 421 ES 422 + Sbjct: 421 SA 422 >BH3749 [M] COG0766 UDP-N-acetylglucosamine enolpyruvyl transferase Length = 440 Score = 536 bits (1380), Expect = e-152, Method: Compositional matrix adjust. Identities = 271/432 (62%), Positives = 322/432 (74%), Gaps = 5/432 (1%) Query: 1 MSKFIVRGGNRLTGSVKVSGAKNSVLPIIAASLLAEEGVSVIVDAPPLDDVMTISKVLES 60 M K IVRGG +L+GSVKV GAKN+VLP+IAAS+LA G S I D P L DV T+ +VL + Sbjct: 1 MEKIIVRGGRQLSGSVKVEGAKNAVLPVIAASILASRGTSKIYDVPKLADVYTMKEVLRN 60 Query: 61 LGAGVTYQNDVIEVDARNITSYEAPYEWVRKMRASFLVMGPLLSRLGHTRISLPGGCAIG 120 L V Y+N V+A N EAP+E+VRKMRASFLVMGPLL+R+GH RI+LPGGCAIG Sbjct: 61 LNINVEYENGEFVVNATNPLKTEAPFEYVRKMRASFLVMGPLLARVGHARIALPGGCAIG 120 Query: 121 TRPIDQHLKGFEALGAEISLGQGYIEAKSNGRLRGAKVYLDVASVGATENIMMAAALAEG 180 +RPIDQHLKGFEA+GA + +G G+IEAK GRL+G K+YLD SVGATENIMMAAA+AEG Sbjct: 121 SRPIDQHLKGFEAMGATVEIGNGFIEAKVEGRLQGTKIYLDFPSVGATENIMMAAAMAEG 180 Query: 181 TTVIENAAKEPEIVDLANYLNXXXXXXXXXXXXXIRIEGVERLHGVKHHVIPDRIEAGTY 240 TT++ENAA+EPEIV LANYLN IRIEGV+ L G +H VI DRIEAGT+ Sbjct: 181 TTILENAAEEPEIVCLANYLNAMGAKVRGAGTGVIRIEGVDELVGAEHTVISDRIEAGTF 240 Query: 241 MAAAAITGGDVYVEGAIADHLGPVIAKMEEMGVTIIPDENGVRVISDKPLKAVDLKTLPY 300 M AAAITGGDV++EGA+A+HL P+IAKM EMGV I ++NG+RVI LK VD+KT+P+ Sbjct: 241 MVAAAITGGDVFIEGAVAEHLRPLIAKMHEMGVKTIEEDNGIRVIGPDELKPVDIKTMPH 300 Query: 301 PGFPTDMQSQMMALLLRSEGTSVVTETVFENRFMHVDEFHNMNAEIKIEGRSAIVTGNAA 360 PGFPTDMQSQMMALLLR+ GTSV+TETVFENRFMHV+EF MN IKIEGRSAI++G Sbjct: 301 PGFPTDMQSQMMALLLRANGTSVITETVFENRFMHVEEFRRMNGNIKIEGRSAIISGPCQ 360 Query: 361 LVGAKVCATDLRXXXXXXXXXXXXXXTTEVSGTHHIDRGYVNLAEKLSGLGADIWRI--- 417 L GA+V ATDLR T V H+DRGYV+LA KL+ LGADI R+ Sbjct: 361 LQGAEVTATDLRAGAALVLAGLVADGHTRVVELKHVDRGYVDLAGKLARLGADIERVVET 420 Query: 418 --SMEESGAAAP 427 +E A AP Sbjct: 421 EHELENLDAPAP 432 >lin2670 [M] COG0766 UDP-N-acetylglucosamine enolpyruvyl transferase Length = 432 Score = 483 bits (1243), Expect = e-136, Method: Compositional matrix adjust. Identities = 33/417 (7%), Positives = 73/417 (17%), Gaps = 1/417 (0%) Query: 1 MSKFIVRGGNRLTGSVKVSGAKNSVLPIIAASLLAEEGVSVIVDAPPLDDVMTISKVLES 60 MSK + G + S + V +A + ++ K Sbjct: 1 MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRFL 60 Query: 61 LGAGVTYQNDVIEVDARNITSYEAPYEWVRKMRASFLVMGPLLSRLGHTRISLPGGCAIG 120 V A+ + +A + L S+ G I Sbjct: 61 GKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTENKSKFGANAILAVSLANAK 120 Query: 121 TRPIDQHLKGFEALGAEISLGQGYIEAKSNGRLRGAKVYLDVASVGATENIMMAAALAEG 180 + + +E + Y +V E ++ Sbjct: 121 AAAAAKGMPLYEHIAELNGTPGKYSMPVPM-MNIINGGEHADNNVDIQEFMIQPVGAKTV 179 Query: 181 TTVIENAAKEPEIVDLANYLNXXXXXXXXXXXXXIRIEGVERLHGVKHHVIPDRIEAGTY 240 I ++ + + V + Sbjct: 180 KEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGSNAEALAVIAEAVKAAGYELGK 239 Query: 241 MAAAAITGGDVYVEGAIADHLGPVIAKMEEMGVTIIPDENGVRVISDKPLKAVDLKTLPY 300 A+ L K E + ++ ++ Sbjct: 240 DITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHFLEELTKQYPIVSIEDGLDESDWD 299 Query: 301 PGFPTDMQSQMMALLLRSEGTSVVTETVFENRFMHVDEFHNMNAEIKIEGRSAIVTGNAA 360 L+ + T+ + E + + + A Sbjct: 300 GFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMA 359 Query: 361 LVGAKVCATDLRXXXXXXXXXXXXXXTTEVSGTHHIDRGYVNLAEKLSGLGADIWRI 417 R T + K + L + Sbjct: 360 KDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRSDRVAKYNQLIRIEEAL 416 >SA1902 [M] COG0766 UDP-N-acetylglucosamine enolpyruvyl transferase Length = 421 Score = 473 bits (1217), Expect = e-133, Method: Compositional matrix adjust. Identities = 244/420 (58%), Positives = 302/420 (71%), Gaps = 2/420 (0%) Query: 1 MSKFIVRGGNRLTGSVKVSGAKNSVLPIIAASLLAEEGVSVIVDAPPLDDVMTISKVLES 60 M K +++GGN+LTG VKV GAKN+VLPI+ ASLLA + S +V+ P L DV TI+ VL + Sbjct: 1 MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVNVPALSDVETINNVLTT 60 Query: 61 LGAGVTYQND--VIEVDARNITSYEAPYEWVRKMRASFLVMGPLLSRLGHTRISLPGGCA 118 L A VTY+ D + VDA + EAPYE+V KMRAS LVMGPLL+RLGH ++LPGGCA Sbjct: 61 LNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMGPLLARLGHAIVALPGGCA 120 Query: 119 IGTRPIDQHLKGFEALGAEISLGQGYIEAKSNGRLRGAKVYLDVASVGATENIMMAAALA 178 IG+RPI+QH+KGFEALGAEI L G I A + L+G ++LD SVGAT+NI+MAA+LA Sbjct: 121 IGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSIHLDFPSVGATQNIIMAASLA 180 Query: 179 EGTTVIENAAKEPEIVDLANYLNXXXXXXXXXXXXXIRIEGVERLHGVKHHVIPDRIEAG 238 +G T+IENAAKEPEIVDLANY+N I I GVE LHGV+H +IPDRIEAG Sbjct: 181 KGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTITINGVESLHGVEHAIIPDRIEAG 240 Query: 239 TYMAAAAITGGDVYVEGAIADHLGPVIAKMEEMGVTIIPDENGVRVISDKPLKAVDLKTL 298 T + A AIT GD++V GAI +H+ ++ K+EEMGV + E+G+RV ++ L+ VD+KTL Sbjct: 241 TLLIAGAITRGDIFVRGAIKEHMASLVYKLEEMGVELDYQEDGIRVRAEGELQPVDIKTL 300 Query: 299 PYPGFPTDMQSQMMALLLRSEGTSVVTETVFENRFMHVDEFHNMNAEIKIEGRSAIVTGN 358 P+PGFPTDMQSQMMALLL + G VVTETVFENRFMHV EF MNA I +EGRSA + G Sbjct: 301 PHPGFPTDMQSQMMALLLTANGHKVVTETVFENRFMHVAEFKRMNANINVEGRSAKLEGK 360 Query: 359 AALVGAKVCATDLRXXXXXXXXXXXXXXTTEVSGTHHIDRGYVNLAEKLSGLGADIWRIS 418 + L GA+V ATDLR T V+ H+DRGYV+L KL LGADI RI+ Sbjct: 361 SQLQGAQVKATDLRAAAALILAGLVADGKTSVTELTHLDRGYVDLHGKLKQLGADIERIN 420 >SP1966 [M] COG0766 UDP-N-acetylglucosamine enolpyruvyl transferase Length = 427 Score = 411 bits (1057), Expect = e-114, Method: Compositional matrix adjust. Identities = 215/421 (51%), Positives = 287/421 (68%), Gaps = 5/421 (1%) Query: 1 MSKFIVRGG-NRLTGSVKVSGAKNSVLPIIAASLLAEEGVSVIVDAPPLDDVMTISKVLE 59 M K +V+GG NRL GSV + GAKN+VLP++AA++LA EG +V+ + P L DV +++V+ Sbjct: 1 MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQNVPILSDVFIMNQVVG 60 Query: 60 SLGAGVTYQND--VIEVDARNITSYEAPYEWVRKMRASFLVMGPLLSRLGHTRISLPGGC 117 L A V + + +++VDA + EAPY++V KMRAS +V+GP+L+R+GH ++S+PGGC Sbjct: 61 GLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVLGPILARVGHAKVSMPGGC 120 Query: 118 AIGTRPIDQHLKGFEALGAEISLGQGYIEAKSNGRLRGAKVYLDVASVGATENIMMAAAL 177 IG+RPID HLKG EA+G +IS GYIEAK+ RL GA +Y+D SVGAT+N+MMAA L Sbjct: 121 TIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAE-RLHGAHIYMDFPSVGATQNLMMAATL 179 Query: 178 AEGTTVIENAAKEPEIVDLANYLNXXXXXXXXXXXXXIRIEGVERLHGVKHHVIPDRIEA 237 A+G TVIENAA+EPEIVDLA LN I I GVE+LHG H+V+ DRIEA Sbjct: 180 ADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETITITGVEKLHGTTHNVVQDRIEA 239 Query: 238 GTYMAAAAITGGDVYVEGAIADHLGPVIAKMEEMGVTIIPDENGVRVISD-KPLKAVDLK 296 GT+M AAA+TGGDV + A+ +H P+IAK+ EMGV +I ++ G+RV S + LKAV +K Sbjct: 240 GTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVEVIEEDEGIRVRSQLENLKAVHVK 299 Query: 297 TLPYPGFPTDMQSQMMALLLRSEGTSVVTETVFENRFMHVDEFHNMNAEIKIEGRSAIVT 356 TLP+PGFPTDMQ+Q AL+ ++G S + ETVFENRF H++E M +I +A + Sbjct: 300 TLPHPGFPTDMQAQFTALMTVAKGESTMVETVFENRFQHLEEMRRMGLHSEIIRDTARIV 359 Query: 357 GNAALVGAKVCATDLRXXXXXXXXXXXXXXTTEVSGTHHIDRGYVNLAEKLSGLGADIWR 416 G L GA+V +TDLR T V H+DRGY EKL+ LGA I R Sbjct: 360 GGQPLQGAEVLSTDLRASAALILTGLVAQGETVVGKLVHLDRGYYGFHEKLAQLGAKIQR 419 Query: 417 I 417 I Sbjct: 420 I 420 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.133 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 32,092,202 Number of extensions: 1386832 Number of successful extensions: 6940 Number of sequences better than 1.0e-05: 21 Number of HSP's gapped: 6881 Number of HSP's successfully gapped: 21 Length of query: 460 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 350 Effective length of database: 25,691,691 Effective search space: 8992091850 Effective search space used: 8992091850 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)