BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0063.0005.p (465 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0021 [M] COG1686 D-alanyl-D-alanine carboxypeptidase 288 1e-77 lin2897 [M] COG1686 D-alanyl-D-alanine carboxypeptidase 272 9e-73 BS_dacA [M] COG1686 D-alanyl-D-alanine carboxypeptidase 260 3e-69 BH1535 [M] COG1686 D-alanyl-D-alanine carboxypeptidase 236 9e-62 PA3999 [M] COG1686 D-alanyl-D-alanine carboxypeptidase 205 1e-52 >BH0021 [M] COG1686 D-alanyl-D-alanine carboxypeptidase Length = 444 Score = 288 bits (738), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 165/407 (40%), Positives = 247/407 (60%), Gaps = 27/407 (6%) Query: 71 LKLRSAVLLEPTTGEVLLSLNADVPLPPASMTKMMTEYLVTEAVNKGQLSWDQKVVVQEN 130 L+ SA+L++ +G++L N D LP ASMTKMM+EYL+ EA+N+G++SWDQ+V + + Sbjct: 34 LEAESAILVDAESGKILYGKNIDAVLPTASMTKMMSEYLILEAINEGEISWDQEVPISDY 93 Query: 131 ASK-----QIGSRIFLAQGDEHTVKELYIAMAVGSANDATVALAEEVAGSEQEFVAMMNE 185 + Q+ S + L Q + +TVKELY ++A+ SAN +T+ALAE +AGSE FV MNE Sbjct: 94 VRELSLYVQL-SNVPLRQDETYTVKELYESVAIYSANASTIALAELIAGSESAFVQRMNE 152 Query: 186 TAQKMGMKTAYFINSTGLNRADMPAKFQPDTDR--ETVMSAMDAAILAKHIVTDHPDFTD 243 +++G++ F+NS+GLN D+ TD E VMSA A LA +++ D+P+ + Sbjct: 153 KGEELGLEDFKFVNSSGLNNKDLLGNHPEGTDANAENVMSARATAKLAYNLLKDYPEVLE 212 Query: 244 FTTIQSYKFR--DRDKAPMINYNWMLEANKNVTNFKAYAYPGLDGLKTGHTNDAGNCFTG 301 +I FR + ++ M N+NWML+ + + Y G+DGLKTG+T +AGNCFTG Sbjct: 213 TASIPEKTFRAGEGEEIEMENWNWMLKGIR-----PQHDYEGIDGLKTGYTAEAGNCFTG 267 Query: 302 TAVRDGMRLISVVMGADSEPHRFTETKKVLDFGFNNFEVKQVVAPKAVIAGNETVPVLKG 361 TA R+GMRLISVVM ++ RF ET K+LD+GFNNF +V+ A +T+PV G Sbjct: 268 TAERNGMRLISVVMRTETRDDRFKETAKLLDYGFNNFTHTEVLPAGFQPAEAQTLPVSSG 327 Query: 362 KNKTVPVVTDAGVTFIVPKGTASPQIKTAVVINDPAT------LVAPIAGASKVGKVTYS 415 K + V + T + +V G ++ VV D ++ L AP+ VG++T + Sbjct: 328 KEREVSISTSEPLHAVVRNG--EEELYDPVVTIDESSLDEDGGLEAPVEAGQAVGQITLT 385 Query: 416 YQVEGIT----QVQEKTVNLITAEEAEKAGWFKLLMRAIGEFFGDLF 458 Y +G T + Q+ L+T + E+AGWF LLMR IG FF +++ Sbjct: 386 YSGDGETAYLYEDQQGGTTLVTDADVERAGWFSLLMRGIGGFFSNVW 432 >lin2897 [M] COG1686 D-alanyl-D-alanine carboxypeptidase Length = 445 Score = 272 bits (695), Expect = 9e-73, Method: Compositional matrix adjust. Identities = 165/405 (40%), Positives = 243/405 (60%), Gaps = 31/405 (7%) Query: 75 SAVLLEPTTGEVLLSLNADVPLPPASMTKMMTEYLVTEAVNKGQLSWDQKVVVQENASKQ 134 +A+ +E +TG++L S +AD + ASMTKMM EYL+ EA++KGQL WD KV + E A K Sbjct: 41 AAIAIEESTGKILYSKDADKLMGIASMTKMMDEYLLLEAIDKGQLKWDDKVTISEYAYKV 100 Query: 135 IG----SRIFLAQGDEHTVKELYIAMAVGSANDATVALAEEVAGSEQEFVAMMNETAQKM 190 S + L G+E++V+ELY AMA+ SAN A +A++E++AGSE+EFV +MN+ A+++ Sbjct: 101 SQDTSLSNVPLRLGEEYSVQELYEAMAIYSANGAAIAISEKIAGSEKEFVDLMNKKAEEL 160 Query: 191 GMKTAYFINSTGLNRADMPAKFQPD--TDRETVMSAMDAAILAKHIVTDHPDF--TDFTT 246 + F+NSTGLN D+ Q TD E M+A A LAKH++ D+PD T TT Sbjct: 161 KLGDHQFVNSTGLNNEDLKGGQQVGGPTD-ENKMTARGMAKLAKHLIDDYPDVLKTASTT 219 Query: 247 IQSYKFRDRDKAPMINYNWMLEANKNVTNFKAYAYPGLDGLKTGHTNDAGNCFTGTAVRD 306 + ++ D+ M N+NW+L Y G+DGLKTG T+ AG C T TAV+D Sbjct: 220 KKEFRKGTSDQIDMSNWNWLLPG-------LIYGRKGVDGLKTGTTDYAGMCLTATAVQD 272 Query: 307 GMRLISVVM---GADSEPH---RFTETKKVLDFGFNNFEVKQVVAPKAVIAGNETVPVLK 360 GMR+++VV+ G H RF ET K+LD+ FNNF+VK+V + + T+ V K Sbjct: 273 GMRVVTVVLHANGGGKGEHTSARFDETNKMLDYAFNNFKVKEVQKAGSKVKIPSTIAVDK 332 Query: 361 GKNKTVPVVTDAGVTFIVPKGTASPQIKTAVVINDPATLVAPIAGASKVGKVTYSY---- 416 GK TV ++T V +VPK P++ T V + + TL AP+ ++VG++ S Sbjct: 333 GKEDTVGLITKDAVKLVVPKNDNEPKLSTKVKLKEK-TLEAPVKKNTEVGEMEVSLKDGD 391 Query: 417 QVEGITQVQEKTVNLITAEEAEKAGWFKLLMRAIGEFFGDLFKGI 461 ++ + Q + + ++TA + EKA WF L +A+G F FKGI Sbjct: 392 KLGYLDGTQTENIKVLTAGDVEKANWFALSAQAVGSF----FKGI 432 >BS_dacA [M] COG1686 D-alanyl-D-alanine carboxypeptidase Length = 443 Score = 260 bits (665), Expect = 3e-69, Method: Compositional matrix adjust. Identities = 165/402 (41%), Positives = 242/402 (60%), Gaps = 23/402 (5%) Query: 75 SAVLLEPTTGEVLLSLNADVPLPPASMTKMMTEYLVTEAVNKGQLSWDQKVV----VQEN 130 +A+++E ++G++L S NAD LP ASMTKMMTEYL+ EA+++G++ WDQ V E Sbjct: 42 AAIMIEASSGKILYSKNADKRLPIASMTKMMTEYLLLEAIDQGKVKWDQTYTPDDYVYEI 101 Query: 131 ASKQIGSRIFLAQGDEHTVKELYIAMAVGSANDATVALAEEVAGSEQEFVAMMNETAQKM 190 + S + L + ++TVKELY A A+ SAN A +A+AE VAGSE +FV MN A+++ Sbjct: 102 SQDNSLSNVPLRKDGKYTVKELYQATAIYSANAAAIAIAEIVAGSETKFVEKMNAKAKEL 161 Query: 191 GMKTAYFINSTGLNRADMPAKFQPD---TDRETVMSAMDAAILAKHIVTDHPDFTDFTTI 247 G+ F+N+TGL D+ QP+ + E+ +SA D A+LA H++TD+P+ + ++I Sbjct: 162 GLTDYKFVNATGLENKDLHG-HQPEGTSVNEESEVSAKDMAVLADHLITDYPEILETSSI 220 Query: 248 QSYKFRD--RDKAPMINYNWMLEANKNVTNFKAYAYPGLDGLKTGHTNDAGNCFTGTAVR 305 KFR+ D+ M N+N+ML+ Y +DGLKTG T+ AG+CFTGTA R Sbjct: 221 AKTKFREGTDDEMDMPNWNFMLKG-----LVSEYKKATVDGLKTGSTDSAGSCFTGTAER 275 Query: 306 DGMRLISVVMGADSEPH--RFTETKKVLDFGFNNFEVKQVVAPKAVIAGNETVPVLKGKN 363 +GMR+I+VV+ A H RF ETKK+ D+ F+NF +K++ A + G++T+ V KGK Sbjct: 276 NGMRVITVVLNAKGNLHTGRFDETKKMFDYAFDNFSMKEIYAEGDQVKGHKTISVDKGKE 335 Query: 364 KTVPVVTDAGVTFIVPKGTASPQIKTAVVINDPATLVAPIAGASKVGKVTYSYQVE---- 419 K V +VT+ + V G K V +N L AP+ +KVGK+T Y + Sbjct: 336 KEVGIVTNKAFSLPVKNG-EEKNYKAKVTLN-KDNLTAPVKKGTKVGKLTAEYTGDEKDY 393 Query: 420 GITQVQEKTVNLITAEEAEKAGWFKLLMRAIGEFFGDLFKGI 461 G V+L+T E EKA WF L MR+IG FF ++ I Sbjct: 394 GFLNSDLAGVDLVTKENVEKANWFVLTMRSIGGFFAGIWGSI 435 >BH1535 [M] COG1686 D-alanyl-D-alanine carboxypeptidase Length = 387 Score = 236 bits (601), Expect = 9e-62, Method: Compositional matrix adjust. Identities = 137/382 (35%), Positives = 223/382 (58%), Gaps = 34/382 (8%) Query: 75 SAVLLEPTTGEVLLSLNADVPLPPASMTKMMTEYLVTEAVNKGQLSWDQKVVVQENASKQ 134 SA+++E TG+VL N+D LPPASMTK+MT L+ EA++ G++++D V ENA+ Sbjct: 37 SAIVIERDTGQVLFEKNSDEKLPPASMTKIMTLLLIMEAIDSGKITYDDMVRTSENAASM 96 Query: 135 IGSRIFLAQGDEHTVKELYIAMAVGSANDATVALAEEVAGSEQEFVAMMNETAQKMGMKT 194 GS++FL G+E +V+++ A+A+ S NDA+VA+AE +AG+E+EFV MMNE A+++G+K Sbjct: 97 GGSQVFLEPGEEMSVRDMIKAIAIASGNDASVAMAEHLAGTEEEFVGMMNEKARQLGLKN 156 Query: 195 AYFINSTGLNRADMPAKFQPDTDRETVMSAMDAAILAKHIVTDHPDFTDFTTI-QSYKFR 253 F+N+ GL P+ D T SA D A+++K ++ + D T+FT++ + Y + Sbjct: 157 TNFVNTNGL----------PEKDHYT--SAYDLAMISKELL-KYEDITEFTSVYEDYLRK 203 Query: 254 DRDKAPMINYNWMLEANKNVTNFKAYAYPGLDGLKTGHTNDAGNCFTGTAVRDGMRLISV 313 DK W++ N+ V YPG+DGLKTG T +A C T TAV++GMR+I+V Sbjct: 204 GTDKE-----FWLVNTNRLVK-----FYPGVDGLKTGFTKEAKYCLTATAVKNGMRVITV 253 Query: 314 VMGADSEPHRFTETKKVLDFGFNNFEVKQVVAPKAVIAGNETVPVLKGKNKTVPVVTDAG 373 VMGA + R ++ ++LDF F+ ++ QV + + V KG K+VP+ T Sbjct: 254 VMGAPTPKERNSQITEMLDFAFSQYQTHQVYERDTYMM---DLNVRKGNKKSVPIFTSES 310 Query: 374 VTFIVPKGTASPQIKTAVVINDPATLVAPIAGASKVGKVTYSYQVEGITQVQEKTVNLIT 433 V+ + KG + + V + +L+AP+ VG + E +++ LI Sbjct: 311 VSLVTKKGESIDNVTERVEWKE--SLLAPVHKGDVVGTLYLERDGEILSETP-----LIA 363 Query: 434 AEEAEKAGWFKLLMRAIGEFFG 455 E+ A ++++ R +G+F G Sbjct: 364 GEDVASASFWQMFKRIVGKFGG 385 >PA3999 [M] COG1686 D-alanyl-D-alanine carboxypeptidase Length = 386 Score = 205 bits (521), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 137/390 (35%), Positives = 207/390 (53%), Gaps = 41/390 (10%) Query: 71 LKLRSAVLLEPTTGEVLLSLNADVPLPPASMTKMMTEYLVTEAVNKGQLSWDQKVVVQEN 130 L +S VL++ +G+VL+ N D LPPAS+TK+MT Y+ T+ + G++ + V V E+ Sbjct: 35 LAAKSYVLMDGESGQVLVENNGDQRLPPASLTKLMTAYIATKEIEAGRIGENDLVTVSEH 94 Query: 131 ASKQIGSRIFLAQGDEHTVKELYIAMAVGSANDATVALAEEVAGSEQEFVAMMNETAQKM 190 A + GSR+F+ G + +V +L + + S NDA+VALAE +AGSE F MMN TAQK+ Sbjct: 95 AWRTGGSRMFIKVGSQVSVSDLLHGIIIQSGNDASVALAEHIAGSEDAFADMMNTTAQKL 154 Query: 191 GMKTAYFINSTGLNRADMPAKFQPDTDRETVMSAMDAAILAKHIVTDHPDFTDFTTIQSY 250 G+ ++F+++TGL P+ D + SA D A+LA+ I+ P + + Sbjct: 155 GLTNSHFMDATGL----------PNPDHYS--SARDMAVLARAIIYGEPSHYAIYAQKEF 202 Query: 251 KFRDRDKAPMINYNWMLEANKNVTNFKAYAYPGLDGLKTGHTNDAGNCFTGTAVRDGMRL 310 + + K P N N +L +K V DGLKTGHT++AG C +AVRDG R+ Sbjct: 203 LW-NNIKQP--NRNLLLWRDKTV-----------DGLKTGHTDEAGYCLVASAVRDGQRM 248 Query: 311 ISVVMGADSEPHRFTETKKVLDFGFNNFEVKQVVAPKAVIAGNETVP--VLKGKNKTVPV 368 I+VV G +SE R ET+K+L +GF FE + G E V KG V Sbjct: 249 IAVVFGTNSEQARAAETQKLLTYGFRFFESRNFYKK-----GTELTKGLVWKGSEHEVKA 303 Query: 369 VTDAGVTFIVPKGTASPQIKTAVVINDPATLVAPIAGASKVGKVTYSYQVEGITQVQEKT 428 +T +P+G Q A ++ +P L+API +GKV + I ++ Sbjct: 304 GLAEDLTMTLPRGQM--QKLQASMVLEP-QLMAPIQQGQVIGKVEVKLDDKVI-----RS 355 Query: 429 VNLITAEEAEKAGWFKLLMRAIGEFFGDLF 458 +L+ E+ G+F+ L +I FF LF Sbjct: 356 ADLVALNAVEEGGFFRRLWDSIRLFFYGLF 385 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.130 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,936,503 Number of extensions: 1256395 Number of successful extensions: 3408 Number of sequences better than 1.0e-05: 72 Number of HSP's gapped: 3266 Number of HSP's successfully gapped: 73 Length of query: 465 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 355 Effective length of database: 25,691,691 Effective search space: 9120550305 Effective search space used: 9120550305 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 118 (50.1 bits)