BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0063.0002.p (194 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0472 [R] COG0110 Acetyltransferase (isoleucine patch superfam... 157 1e-38 MTH1906 [R] COG1287 Uncharacterized membrane protein, required f... 128 5e-30 YMR095c [H] COG0311 Predicted glutamine amidotransferase involve... 93 3e-19 YNL334c [H] COG0311 Predicted glutamine amidotransferase involve... 90 2e-18 YFL060c [H] COG0311 Predicted glutamine amidotransferase involve... 86 3e-17 >STM0472 [R] COG0110 Acetyltransferase (isoleucine patch superfamily) Length = 183 Score = 157 bits (396), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 0/188 (0%), Positives = 0/188 (0%), Gaps = 1/188 (0%) Query: 1 MKIGVLALQGAVTEHIVSIEKTGAQGLPIKRAXXXXXXXXXXXXXXXSTTIGKLMRKYGF 60 M + + + + L R Sbjct: 1 MSDEKQKMIAGALYCPTDETLCQDRLRARQLIHQYNYTTPDEINKRQAILRDLLGRCEDA 60 Query: 61 IEAIRDFAGRGKPVFGTCAGMIVLAKRIDGGEPAHLELMDITVARNAFGRQRESFECDLD 120 G +F + + P H+ + + Sbjct: 61 YIEPSFRCDYGYNIFLGHSFYANFDCVMLDVCPIHIGDNCMLAPGVHIYTATHPLDAVER 120 Query: 121 VKGI-DEPVRAVFIRAPLITEVGPDVDVLTVYKDEIVTARQGNLLVSSFHPELTDDYRLH 179 G + + + V + + V + R+ Sbjct: 121 NSGRELGKPVTIGNNVWIGGRAVVNPGVTIGDNVVVASGAVVIKNVPPDVVVGGNPARII 180 Query: 180 QYFADMVE 187 Sbjct: 181 188 >MTH1906 [R] COG1287 Uncharacterized membrane protein, required for N-linked glycosylation Length = 192 Score = 128 bits (322), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 79/189 (41%), Positives = 112/189 (59%), Gaps = 14/189 (7%) Query: 1 MKIGVLALQGAVTEHIV----SIEKTG--AQGLPIKRAXXXXXXXXXXXXXXXSTTIGKL 54 ++IG+LALQG V+EH+ ++E+ G A+ + ++ A ST IG+L Sbjct: 2 IRIGILALQGDVSEHLEMTRRTVEEMGIDAEVVRVRTAEEASTVDAIIISGGESTVIGRL 61 Query: 55 MRKYGFIEAIRDFAGR-GKPVFGTCAGMIVLAKRIDGGEPAHLELMDITVARNAFGRQRE 113 M + G I+D R KPV GTCAGM++LA D +P L L+D+ V RNAFGRQR+ Sbjct: 62 MEETG----IKDVIIREKKPVMGTCAGMVLLADETDYEQPL-LGLIDMKVKRNAFGRQRD 116 Query: 114 SFECDLDVKGIDEPVRAVFIRAPLITEVGPDVDVLTVYKDEIVTARQGNLLVSSFHPELT 173 SFE ++D+ G +FIRAP + EVG V+VL+ D I+ + G L +FHPEL Sbjct: 117 SFEDEIDILG--RKFHGIFIRAPAVLEVGEGVEVLSELDDMIIAVKDGCNLALAFHPELG 174 Query: 174 DDYRLHQYF 182 +D LH+YF Sbjct: 175 EDTGLHEYF 183 >YMR095c [H] COG0311 Predicted glutamine amidotransferase involved in pyridoxine biosynthesis Length = 252 Score = 92.8 bits (229), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 16/203 (7%), Positives = 39/203 (19%), Gaps = 24/203 (11%) Query: 3 IGVLALQGAVTEHIVSIEKTGAQ---GLPI-----KRAXXXXXXXXXXXXXXXSTTIGKL 54 A++ + ++E +R + L Sbjct: 16 RSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSPAMIAEARSAL 75 Query: 55 MRKYGFIEAIRDFAGRG-KPVFGTCAGMIVLAKRIDGGEP--AHLELMDITVARNAFGRQ 111 IR++ + A + L + + L + + Sbjct: 76 PDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWL 135 Query: 112 RESF--ECDLD--VKGIDEPVRAVFIRAPLITEVGPDVDVLTVY------KDEIVTARQG 161 + ++ D R ++ + Sbjct: 136 EPTHVLMREVAWEQNYPDR-GREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAII 194 Query: 162 -NLLVSSFHPELTD-DYRLHQYF 182 + + P L D Q F Sbjct: 195 DWVTATGLRPWLQDLTESEQQLF 217 >YNL334c [H] COG0311 Predicted glutamine amidotransferase involved in pyridoxine biosynthesis Length = 252 Score = 90.1 bits (222), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 6/209 (2%), Positives = 22/209 (10%), Gaps = 31/209 (14%) Query: 3 IGVLALQGAVTEHIVSIEKTGAQG----------LPIKRAXXXXXXXXXXXXXXXSTTIG 52 L L + ++E Sbjct: 5 NPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSS 64 Query: 53 KLMRKYGFIEAIRDFAGR-GKPVFGTCAGMIVLAKRIDGGEP--AHLELMDITVARNAFG 109 M + + ++ + + N G Sbjct: 65 PAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQG 124 Query: 110 RQRESFE--CDLDV---KGIDEPVRAVFIRAPLITEVGPDVDVLTVYK---------DEI 155 + + Sbjct: 125 VLAVQMPDNWLEPTHVLMREVAW--EQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTT 182 Query: 156 VTARQ-GNLLVSSFHPELT-DDYRLHQYF 182 + + + + + Sbjct: 183 YYHQMPSHQAIIDWVTATGLRPWLQDLTE 211 >YFL060c [H] COG0311 Predicted glutamine amidotransferase involved in pyridoxine biosynthesis Length = 252 Score = 86.3 bits (212), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 6/209 (2%), Positives = 22/209 (10%), Gaps = 31/209 (14%) Query: 3 IGVLALQGAVTEHIVSIEKTGAQG----------LPIKRAXXXXXXXXXXXXXXXSTTIG 52 L L + ++E Sbjct: 5 NPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSS 64 Query: 53 KLMRKYGFIEAIRDFAGR-GKPVFGTCAGMIVLAKRIDGGEP--AHLELMDITVARNAFG 109 M + + ++ + + N G Sbjct: 65 PAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQG 124 Query: 110 RQRESFE--CDLDV---KGIDEPVRAVFIRAPLITEVGPDVDVLTVYK---------DEI 155 + + Sbjct: 125 VLAVQMPDNWLEPTHVLMREVAW--EQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTT 182 Query: 156 VTARQ-GNLLVSSFHPELT-DDYRLHQYF 182 + + + + + Sbjct: 183 YYHQMPSHQAIIDWVTATGLRPWLQDLTE 211 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.139 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 13,135,152 Number of extensions: 477972 Number of successful extensions: 778 Number of sequences better than 1.0e-05: 5 Number of HSP's gapped: 763 Number of HSP's successfully gapped: 5 Length of query: 194 Length of database: 38,378,431 Length adjustment: 101 Effective length of query: 93 Effective length of database: 26,729,697 Effective search space: 2485861821 Effective search space used: 2485861821 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 113 (48.1 bits)