BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0061.0005.p (306 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YGL031c [J] COG2075 Ribosomal protein L24E 152 8e-37 YKL218c [E] COG1171 Threonine dehydratase 152 8e-37 L24277 [LR] COG0494 NTP pyrophosphohydrolases including oxidativ... 152 8e-37 ML2088 [Q] COG2124 Cytochrome P450 152 8e-37 YNL229c [O] COG0625 Glutathione S-transferase 152 8e-37 >YGL031c [J] COG2075 Ribosomal protein L24E Length = 311 Score = 152 bits (383), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 96/281 (34%), Positives = 145/281 (51%), Gaps = 31/281 (11%) Query: 6 KTFLRHVREGFKNVFRNGWMSVASITXXXXXXXXXXXXXXXXXXXXAIADKADSQVQINV 65 + F +H+ E KN+ RNGWM+VA+++ I++ ++ V+I Sbjct: 3 RNFFKHLLESLKNLKRNGWMTVAAVSSVTITLVLVGLFLSVILNTSKISNGIENNVRIVA 62 Query: 66 HLALNT-DQKMR--------ETLEN--------EIGSMPEVSKVEFVSKEQGLKEFREDM 108 ++ L+ DQ+ + +T+ N I +P V VEF SKE LKE + + Sbjct: 63 YMKLDVHDQETKIQDPKDNKKTINNPDHLKIYDSIKKVPNVKTVEFSSKEAQLKELTKAL 122 Query: 109 GPDAAELLEGFDEDNNPLPDKLLVEVIQPTTVPFVAEKIEALNKTHEEKPIYKVNYGKGS 168 G + F D NPL D +VE +P V V KI L+ I K YG + Sbjct: 123 GNN----WNLFKGDANPLYDAYIVEADKPANVKSVTSKISKLSG------IEKATYGGAN 172 Query: 169 VETLFKVTKAVRNIGFIFVAGLALMSMFLISNTIRVTILARRKEIGIMKLVGATNYFIRW 228 LF++ + +R G L +++FLISNTIR+TIL+R++EI IM+LVGA N +IRW Sbjct: 173 TAKLFQIAQGIRTWGTAAAILLIFVAVFLISNTIRITILSRQREIQIMRLVGAKNGYIRW 232 Query: 229 PFFIEGALIGLIGS----LVTSGALYAGYSSLVSSVQGDPM 265 PFF+EGA +GL+GS L+ + A + SL S+Q + Sbjct: 233 PFFLEGAWVGLLGSIIPGLIVAWAYRLAFVSLTPSLQSQKL 273 >YKL218c [E] COG1171 Threonine dehydratase Length = 346 Score = 152 bits (383), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 16/281 (5%), Positives = 48/281 (17%), Gaps = 31/281 (11%) Query: 6 KTFLRHVREGFKNVFRNGWMSVASITXXXXXXXXXXXXXXXXXXXXAIADKADSQVQINV 65 + H + + Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 66 HLALNT-DQKMR--------ETLEN--------EIGSMPEVSKVEFVSKEQGLKEFREDM 108 L + + G P+ + +E + Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQ 122 Query: 109 GPDAAELLEGFDEDNNPLPDKLLVEVIQPTTVPFVAEKIEALNKTHEEKPIYKVNYGKGS 168 L+ + P ++ T A + G Sbjct: 123 TDY----LDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLD------ALAEYQRAGK 172 Query: 169 VETLFKVTKAVRNIGFIFVAGLALMSMFLISNTIRVTILARRKEIGIMKLVGATNYFIRW 228 + + + + +++ ++L R E+G+ ++ + Sbjct: 173 IRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLA 232 Query: 229 PFFIEGALIGLIGS----LVTSGALYAGYSSLVSSVQGDPM 265 + + + + S Q Sbjct: 233 YSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKA 273 >L24277 [LR] COG0494 NTP pyrophosphohydrolases including oxidative damage repair enzymes Length = 673 Score = 152 bits (383), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 17/281 (6%), Positives = 41/281 (14%), Gaps = 31/281 (11%) Query: 6 KTFLRHVREGFKNVFRNGWMSVASITXXXXXXXXXXXXXXXXXXXXAIADKADSQVQINV 65 + F S IA + Sbjct: 3 LNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQARHIAAVTFTNKAARE 62 Query: 66 HLALNT-DQKMR--------ETLEN--------EIGSMPEVSKVEFVSKEQGLKEFREDM 108 + + + F +Q Sbjct: 63 MKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMKANFSLFDDTDQLALLKELTE 122 Query: 109 GPDAAELLEGFDEDNNPLPDKLLVEVIQPTTVPFVAEKIEALNKTHEEKPIYKVNYGKGS 168 G + + + T A I ++ I+ YG Sbjct: 123 GLI----EDDKVLLQQLISTISNWKNDLKTPSQAAASAIGERDR------IFAHCYGLYD 172 Query: 169 VETLFKVTKAVRNIGFIFVAGLALMSMFLISNTIRVTILARRKEIGIMKLVGATNYFIRW 228 ++ + L ++ L + + Sbjct: 173 AHLKACNVLDFDDLILLPTLLLQANEEVRKRWQNKIRYLLVDEYQDTNTSQYELVKLLVG 232 Query: 229 PFFIEGALIGLIGS----LVTSGALYAGYSSLVSSVQGDPM 265 + S S +++ + Sbjct: 233 SRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKL 273 >ML2088 [Q] COG2124 Cytochrome P450 Length = 311 Score = 152 bits (383), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 96/281 (34%), Positives = 145/281 (51%), Gaps = 31/281 (11%) Query: 6 KTFLRHVREGFKNVFRNGWMSVASITXXXXXXXXXXXXXXXXXXXXAIADKADSQVQINV 65 + F +H+ E KN+ RNGWM+VA+++ I++ ++ V+I Sbjct: 3 RNFFKHLLESLKNLKRNGWMTVAAVSSVTITLVLVGLFLSVILNTSKISNGIENNVRIVA 62 Query: 66 HLALNT-DQKMR--------ETLEN--------EIGSMPEVSKVEFVSKEQGLKEFREDM 108 ++ L+ DQ+ + +T+ N I +P V VEF SKE LKE + + Sbjct: 63 YMKLDVHDQETKIQDPKDNKKTINNPDHLKIYDSIKKVPNVKTVEFSSKEAQLKELTKAL 122 Query: 109 GPDAAELLEGFDEDNNPLPDKLLVEVIQPTTVPFVAEKIEALNKTHEEKPIYKVNYGKGS 168 G + F D NPL D +VE +P V V KI L+ I K YG + Sbjct: 123 GNN----WNLFKGDANPLYDAYIVEADKPANVKSVTSKISKLSG------IEKATYGGAN 172 Query: 169 VETLFKVTKAVRNIGFIFVAGLALMSMFLISNTIRVTILARRKEIGIMKLVGATNYFIRW 228 LF++ + +R G L +++FLISNTIR+TIL+R++EI IM+LVGA N +IRW Sbjct: 173 TAKLFQIAQGIRTWGTAAAILLIFVAVFLISNTIRITILSRQREIQIMRLVGAKNGYIRW 232 Query: 229 PFFIEGALIGLIGS----LVTSGALYAGYSSLVSSVQGDPM 265 PFF+EGA +GL+GS L+ + A + SL S+Q + Sbjct: 233 PFFLEGAWVGLLGSIIPGLIVAWAYRLAFVSLTPSLQSQKL 273 >YNL229c [O] COG0625 Glutathione S-transferase Length = 311 Score = 152 bits (383), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 96/281 (34%), Positives = 145/281 (51%), Gaps = 31/281 (11%) Query: 6 KTFLRHVREGFKNVFRNGWMSVASITXXXXXXXXXXXXXXXXXXXXAIADKADSQVQINV 65 + F +H+ E KN+ RNGWM+VA+++ I++ ++ V+I Sbjct: 3 RNFFKHLLESLKNLKRNGWMTVAAVSSVTITLVLVGLFLSVILNTSKISNGIENNVRIVA 62 Query: 66 HLALNT-DQKMR--------ETLEN--------EIGSMPEVSKVEFVSKEQGLKEFREDM 108 ++ L+ DQ+ + +T+ N I +P V VEF SKE LKE + + Sbjct: 63 YMKLDVHDQETKIQDPKDNKKTINNPDHLKIYDSIKKVPNVKTVEFSSKEAQLKELTKAL 122 Query: 109 GPDAAELLEGFDEDNNPLPDKLLVEVIQPTTVPFVAEKIEALNKTHEEKPIYKVNYGKGS 168 G + F D NPL D +VE +P V V KI L+ I K YG + Sbjct: 123 GNN----WNLFKGDANPLYDAYIVEADKPANVKSVTSKISKLSG------IEKATYGGAN 172 Query: 169 VETLFKVTKAVRNIGFIFVAGLALMSMFLISNTIRVTILARRKEIGIMKLVGATNYFIRW 228 LF++ + +R G L +++FLISNTIR+TIL+R++EI IM+LVGA N +IRW Sbjct: 173 TAKLFQIAQGIRTWGTAAAILLIFVAVFLISNTIRITILSRQREIQIMRLVGAKNGYIRW 232 Query: 229 PFFIEGALIGLIGS----LVTSGALYAGYSSLVSSVQGDPM 265 PFF+EGA +GL+GS L+ + A + SL S+Q + Sbjct: 233 PFFLEGAWVGLLGSIIPGLIVAWAYRLAFVSLTPSLQSQKL 273 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.137 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,153,297 Number of extensions: 719544 Number of successful extensions: 2800 Number of sequences better than 1.0e-05: 329 Number of HSP's gapped: 1853 Number of HSP's successfully gapped: 329 Length of query: 306 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 200 Effective length of database: 26,153,027 Effective search space: 5230605400 Effective search space used: 5230605400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)