BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0061.0002.p (489 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yvjB [M] COG0793 Periplasmic protease 397 e-110 BS_ctpA [M] COG0793 Periplasmic protease 333 4e-91 slr0008 [M] COG0793 Periplasmic protease 222 9e-58 PA5134 [M] COG0793 Periplasmic protease 219 7e-57 slr1751 [M] COG0793 Periplasmic protease 218 1e-56 >BS_yvjB [M] COG0793 Periplasmic protease Length = 480 Score = 397 bits (1019), Expect = e-110, Method: Compositional matrix adjust. Identities = 215/472 (45%), Positives = 292/472 (61%), Gaps = 9/472 (1%) Query: 11 IVAALLCGSLLTLGVTGYADIFXXXXXXXXXXXXXXXXXL---QEKESQKLGTALSLIEG 67 I+A + GS+L G YA I ++K K+ A LI Sbjct: 5 IMAVIAAGSMLFGGAGVYAGINLLEMDKPQTAAVPATAQADSERDKAMDKIEKAYELISN 64 Query: 68 YYYEKVDRTKLIDGAVNGMMEALGDPYSNYMGKETAEKFEESIEGSFSGIGAEVSSENGK 127 Y EKVDR KL++GA+ GM+ L DPYS YM K+TA++F +S++ SF GIGAEV E+GK Sbjct: 65 EYVEKVDREKLLEGAIQGMLSTLNDPYSVYMDKQTAKQFSDSLDSSFEGIGAEVGMEDGK 124 Query: 128 VVIVSPIKGSPAEKAGLKAKDVILSVNGETLEGMELNDAVAKIRGPKGSKATLKIQRTGA 187 ++IVSP K SPAEKAGLK D I+S+NGE++ G +LN AV KIRG KGS ++KIQR G Sbjct: 125 IIIVSPFKKSPAEKAGLKPNDEIISINGESMAGKDLNHAVLKIRGKKGSSVSMKIQRPGT 184 Query: 188 AEPLEYVLTRDDVKLETVYA---KLEKDGVGVIEVTQFSMNTAERFKEELGKLEKQGMKG 244 + L + + R ++ LETV+A K++ VG I ++ FS +TAE F + L +LEK+ ++G Sbjct: 185 KKQLSFRIKRAEIPLETVFASEKKVQGHSVGYIAISTFSEHTAEDFAKALRELEKKEIEG 244 Query: 245 LVIDVRNDPGGVLPVVIDMAEQFVPAGKTIVQVEEK-GKKPEVSPSKGSSKKYPVVVLMN 303 LVIDVR +PGG L V ++ + FV + +Q+ E+ G K + K YPV V+ + Sbjct: 245 LVIDVRGNPGGYLQSVEEILKHFVTKDQPYIQIAERNGDKKRYFSTLTHKKAYPVNVITD 304 Query: 304 KGSASASEILAGALQQSAGAKLIGENSFGKGTVQTSFDKQLGDGSLLKITIAKWLTPNGT 363 KGSASASEILAGAL+++ ++G+ SFGKGTVQ + +GDGS +K+T+ KWLTPNG Sbjct: 305 KGSASASEILAGALKEAGHYDVVGDTSFGKGTVQQAV--PMGDGSNIKLTLYKWLTPNGN 362 Query: 364 WIHGKGIKPDIAVAQPDYFSVAPINKSVSLQYNMNNADVKSAQTMLDGLGYKPGRKDGYF 423 WIH KGI+P IA+ QPDYFS P+ L+ +MNN DVK AQ +L GL + PGR+DGYF Sbjct: 363 WIHKKGIEPTIAIKQPDYFSAGPLQLKEPLKVDMNNEDVKHAQVLLKGLSFDPGREDGYF 422 Query: 424 DTATKDAVKKFQSASKLKATGIVDXXXXXXXXXXXXXXXQNPVNDNQLNRGI 475 K AV FQ +KL TG++D + ND QL + Sbjct: 423 SKDMKKAVMAFQDQNKLNKTGVIDTRTAETLNQQIEKKKSDEKNDLQLQTAL 474 >BS_ctpA [M] COG0793 Periplasmic protease Length = 466 Score = 333 bits (853), Expect = 4e-91, Method: Compositional matrix adjust. Identities = 191/475 (40%), Positives = 287/475 (60%), Gaps = 18/475 (3%) Query: 9 FMIVAALLCGSLLTLGVTGYADIFXXXXXXXXXXXXXXXXXLQEKESQKLGTALSLIEGY 68 F+++ + S LTL +TG + I + + KL A I+ Sbjct: 9 FIVLITAVVASALTLFITGNSSILGQKSASTG-----------DSKFDKLNKAYEQIKSD 57 Query: 69 YYEKVDRTKLIDGAVNGMMEALGDPYSNYMGKETAEKFEESIEGSFSGIGAEVSSENGKV 128 YY+K D KL+DGA+ GM+++L DPYS YM +E A+ F+E+I SF GIGA+V ++G++ Sbjct: 58 YYQKTDDDKLVDGAIKGMIQSLDDPYSTYMDQEQAKSFDETISASFEGIGAQVEEKDGEI 117 Query: 129 VIVSPIKGSPAEKAGLKAKDVILSVNGETLEGMELNDAVAKIRGPKGSKATLKIQRTGAA 188 +IVSPIKGSPAEKAG+K +D I+ VNG++++GM +N+AVA IRG KG+K L++ R G Sbjct: 118 LIVSPIKGSPAEKAGIKPRDQIIKVNGKSVKGMNVNEAVALIRGKKGTKVKLELNRAGVG 177 Query: 189 EPLEYVLTRDDVKLETVYAKLEKDGVGVIEVTQFSMNTAERFKEELGKLEKQGMKGLVID 248 ++ + RD + +ETVY++++ + +G I++T FS TA+ + + LEK+G KG ++D Sbjct: 178 N-IDLSIKRDTIPVETVYSEMKDNNIGEIQITSFSETTAKELTDAIDSLEKKGAKGYILD 236 Query: 249 VRNDPGGVLPVVIDMAEQFVPAGKTIVQVEEKGKKPEVSPSKGSSK-KYPVVVLMNKGSA 307 +R +PGG++ I M+ F+ GK I+QVE K EV ++ K P VVL+N G+A Sbjct: 237 LRGNPGGLMEQAITMSNLFIDKGKNIMQVEYKNGSKEVMKAEKERKVTKPTVVLVNDGTA 296 Query: 308 SASEILAGALQQSAGAKLIGENSFGKGTVQTSFDKQLGDGSLLKITIAKWLTPNGTWIHG 367 SA+EI+A AL +S+ LIGE +FGKGTVQT+ K+ DGS +K+T+AKWLT +G WIH Sbjct: 297 SAAEIMAAALHESSNVPLIGETTFGKGTVQTA--KEYDDGSTVKLTVAKWLTADGEWIHK 354 Query: 368 KGIKPDIAVAQPDYFSVAPINKSVSLQYNMNNADVKSAQTMLDGLGYKPGRKDGYFDTAT 427 KGIKP + PDY + ++ + + +VK AQ ML LGYK + + +D Sbjct: 355 KGIKPQVKAELPDYAKLPYLDADKTYKSGDTGTNVKVAQKMLKALGYKV-KVNSMYDQDF 413 Query: 428 KDAVKKFQSASKLKATGIVDXXXXXXXXXXXXXXXQNPVNDNQLNRGIAEVQKEI 482 VK+FQ KL TGI+ + ND Q+ + I ++KE+ Sbjct: 414 VSVVKQFQKKEKLNETGILTGDTTTKLMIELQKKLSD--NDTQMEKAIETLKKEM 466 >slr0008 [M] COG0793 Periplasmic protease Length = 427 Score = 222 bits (566), Expect = 9e-58, Method: Compositional matrix adjust. Identities = 135/401 (33%), Positives = 217/401 (54%), Gaps = 10/401 (2%) Query: 1 MLKKSTAAFMIVAALLCGSLLTLGVTGYADIFXXXXXXXXXXXXXXXXXLQEKESQKLGT 60 M K++ + + +LL G+L+ LG T A F ++ Sbjct: 1 MGKRTRRFWALAFSLLMGALIYLGNTPSALAFTEEQKLLLQSWRLVNQSYLDETFNHQNW 60 Query: 61 ALSLIEGYYYEKVDRTKLIDGAVNGMMEALGDPYSNYMGKETAEKFEESIEGSFSGIGAE 120 L L E Y + + A+ M+ L +P++ + E + + G SG+G + Sbjct: 61 WL-LREKYVKRPLRNREETYTAIEEMLATLDEPFTRLLRPEQYGNLQVTTTGELSGVGLQ 119 Query: 121 VS--SENGKVVIVSPIKGSPAEKAGLKAKDVILSVNGETLEGMELNDAVAKIRGPKGSKA 178 ++ E ++ I++P+ GSPAE+AGL+ D IL+++G + + L++A A++RGPK +K Sbjct: 120 ININPETNQLEIMAPLAGSPAEEAGLQPHDQILAIDGVDTQTLSLDEAAARMRGPKNTKV 179 Query: 179 TLKIQRTGAAEPLEYVLTRDDVKLETVYAKLEKD----GVGVIEVTQFSMNTAERFKEEL 234 +L+I G P E+ LTR + L V A+L+ VG I ++QFS N + L Sbjct: 180 SLEILSAGTEVPQEFTLTRQLISLSPVAAQLDDSRPGQSVGYIRLSQFSANAYKEVAHAL 239 Query: 235 GKLEKQGMKGLVIDVRNDPGGVLPVVIDMAEQFVPAGKTIVQVEEKGKKPEVSPSKGSSK 294 +LE+QG G ++D+RN+PGG+L ID+A ++P + V +G + + + ++ Sbjct: 240 HQLEEQGADGYILDLRNNPGGLLQAGIDIARLWLPESTIVYTVNRQGTQESFTANGEAAT 299 Query: 295 KYPVVVLMNKGSASASEILAGALQQSAGAKLIGENSFGKGTVQTSFDKQLGDGSLLKITI 354 P+VVL+N+G+ASASEILAGALQ + A L+GE +FGKG +Q+ F+ L DG+ + +T+ Sbjct: 300 DRPLVVLVNQGTASASEILAGALQDNQRATLVGEKTFGKGLIQSLFE--LSDGAGIAVTV 357 Query: 355 AKWLTPNGTWIHGKGIKPDIAVAQPDYFSVAPINKSVSLQY 395 AK+ TP IH GI PD V QP S A I +QY Sbjct: 358 AKYETPQHHDIHKLGIMPDEVVEQP-LISFAEITSPADVQY 397 >PA5134 [M] COG0793 Periplasmic protease Length = 436 Score = 219 bits (559), Expect = 7e-57, Method: Compositional matrix adjust. Identities = 131/331 (39%), Positives = 197/331 (59%), Gaps = 11/331 (3%) Query: 54 ESQKLGTALSLIEGYYYEKVDRTKLIDGAVNGMMEALGDPYSNYMGKETAEKFEESIEGS 113 E + L ++ Y E VD L++ A+ GM+ L DP+S Y+G E + +ES G Sbjct: 45 ELRTFAEVLDRVKAAYVEPVDDKTLLENAIKGMLSNL-DPHSAYLGPEDFAELQESTSGE 103 Query: 114 FSGIGAEVSSENGKVVIVSPIKGSPAEKAGLKAKDVILSVNGETLEGMELNDAVAKIRGP 173 F G+G EV SE+G + +VSPI +PA +AG++ D+I+ ++G+ +G + +AV +RG Sbjct: 104 FGGLGIEVGSEDGFIKVVSPIDDTPAARAGIQPGDLIVQIDGKPTKGQSMTEAVDSMRGK 163 Query: 174 KGSKATLKIQRTGAAEPLEYVLTRDDVKLETVYAKLEKDGVGVIEVTQFSMNTAERFKEE 233 GS TL I R G P + L R +K+++V +++ + G + +TQF +NT E + Sbjct: 164 AGSPITLTIVRDG-GRPFDVELKRAIIKVKSVKSQVLEPGYAYLRITQFQVNTGEEVVKA 222 Query: 234 LGKLEKQG---MKGLVIDVRNDPGGVLPVVIDMAEQFVPAGKTIVQVEEKGKKPEVSPS- 289 L +L K +KGLV+D+RN+PGGVL +++A+ F+ G IV + + E+ S Sbjct: 223 LNQLRKDNKGRLKGLVLDLRNNPGGVLQSAVEVADAFLTKG-LIVYTKGRIANSELRFSA 281 Query: 290 --KGSSKKYPVVVLMNKGSASASEILAGALQQSAGAKLIGENSFGKGTVQTSFDKQLGDG 347 S K P+VVL+N GSASA+EI+AGALQ A L+G +SFGKG+VQT L + Sbjct: 282 DPADPSDKVPLVVLINGGSASAAEIVAGALQDQKRAILMGTDSFGKGSVQTVL--PLNND 339 Query: 348 SLLKITIAKWLTPNGTWIHGKGIKPDIAVAQ 378 LK+T A + TPNG I +GI PDI V + Sbjct: 340 RALKLTTALYYTPNGRSIQAQGIVPDIEVGR 370 >slr1751 [M] COG0793 Periplasmic protease Length = 423 Score = 218 bits (556), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 118/304 (38%), Positives = 191/304 (62%), Gaps = 9/304 (2%) Query: 82 AVNGMMEALGDPYSNYMGKETAEKFEESIEGSFSGIGAEVSSENG--KVVIVSPIKGSPA 139 A+ M+E L DPY+ +M + + G +G+G +++ + K+V+V+PI+ +PA Sbjct: 86 AIREMLEKLNDPYTRFMSPDEFQSMRIDTSGELTGVGIQITQDQDTKKIVVVAPIEDTPA 145 Query: 140 EKAGLKAKDVILSVNGETLEGMELNDAVAKIRGPKGSKATLKIQRTGAAEPLEYVLTRDD 199 AG+ +KDVI ++G++ +GME++DAV IRG G+ L I+R G A +EY LTR Sbjct: 146 YNAGILSKDVITKIDGKSTDGMEVDDAVKLIRGKPGTSVVLTIEREGQA--IEYPLTRTL 203 Query: 200 VKLETVYAKLEKDG---VGVIEVTQFSMNTAERFKEELGKLEKQGMKGLVIDVRNDPGGV 256 +++ V A++E VG I + QFS +E ++ + KLEK+ + G + D+R++PGG+ Sbjct: 204 IEIHPVRAQVEDINGARVGYIRLNQFSAQASEEMRQAVQKLEKENVVGYIFDLRSNPGGL 263 Query: 257 LPVVIDMAEQFVPAGKTIVQVEEKGKKPEVSPSKGSSKKYPVVVLMNKGSASASEILAGA 316 L +D+A ++ G + V+ +G+ + S +K P+VVL++ GSASASEI++GA Sbjct: 264 LYSSVDIARIWLDEGGIVSTVDRRGEVEQQSANKRQLSNRPLVVLVDGGSASASEIVSGA 323 Query: 317 LQQSAGAKLIGENSFGKGTVQTSFDKQLGDGSLLKITIAKWLTPNGTWIHGKGIKPDIAV 376 LQ + A ++G +FGKG VQ+ ++LGDGS + +TIAK+LTPNG I+ GI PD+ V Sbjct: 324 LQDNQRAVIVGTKTFGKGLVQSV--RELGDGSGMAVTIAKYLTPNGRDINKHGIDPDVEV 381 Query: 377 AQPD 380 D Sbjct: 382 ELTD 385 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.311 0.131 0.355 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,211,520 Number of extensions: 1441494 Number of successful extensions: 2777 Number of sequences better than 1.0e-05: 21 Number of HSP's gapped: 2723 Number of HSP's successfully gapped: 21 Length of query: 489 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 379 Effective length of database: 25,691,691 Effective search space: 9737150889 Effective search space used: 9737150889 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 118 (50.1 bits)